rabbit anti vascular endothelial growth factor receptor 3  (Santa Cruz Biotechnology)

 
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    Santa Cruz Biotechnology rabbit anti vascular endothelial growth factor receptor 3
    Effect of pioglitazone on the expression of F4/80 and VEGFR3  in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.
    Rabbit Anti Vascular Endothelial Growth Factor Receptor 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vascular endothelial growth factor receptor 3/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vascular endothelial growth factor receptor 3 - by Bioz Stars, 2020-09
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    1) Product Images from "Pioglitazone increases VEGFR3 expression and promotes activation of M2 macrophages via the peroxisome proliferator-activated receptor γ"

    Article Title: Pioglitazone increases VEGFR3 expression and promotes activation of M2 macrophages via the peroxisome proliferator-activated receptor γ

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2019.9945

    Effect of pioglitazone on the expression of F4/80 and VEGFR3  in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.
    Figure Legend Snippet: Effect of pioglitazone on the expression of F4/80 and VEGFR3 in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.

    Techniques Used: Expressing, In Vivo, Immunofluorescence, Mouse Assay

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    Incubation:

    Article Title: Pioglitazone increases VEGFR3 expression and promotes activation of M2 macrophages via the peroxisome proliferator-activated receptor γ
    Article Snippet: .. Then they were incubated overnight at 4°C with rabbit anti-arginase1 (Arg1; cat. no. sc-20150; 1:400; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit anti-vascular endothelial growth factor receptor 3 (VEGFR3; cat. no. sc-321; 1:400; Santa Cruz Biotechnology, Inc.), mouse anti-inducible nitric oxide synthase (iNOS; cat. no. sc-7271; 1:200; Santa Cruz Biotechnology, Inc.) and rabbit anti-α-tubulin (1:5,000; cat. no. ab18251; Abcam, Cambridge, MA, USA). .. The membranes containing tissue protein were incubated overnight at 4°C with rabbit anti-α-smooth muscle actin (SMA; cat. no. ab5694; 1:1,000; Abcam), rabbit anti-platelet-derived growth factor receptor (PDGFR)-β (cat. no. ab32570; 1:1,000; Abcam), rabbit anti-PPARγ (cat. no. AP20705a; 1:1,000; Abgent, Inc., San Diego, CA, USA), rabbit anti-phosphorylated (p)-PPARγ (cat. no. ab195925; 1:1,000; Abcam) and mouse anti-GAPDH (1:3,000; Abcam; cat. no. ab8245).

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    Santa Cruz Biotechnology rabbit anti vascular endothelial growth factor receptor 3
    Effect of pioglitazone on the expression of F4/80 and VEGFR3  in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.
    Rabbit Anti Vascular Endothelial Growth Factor Receptor 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vascular endothelial growth factor receptor 3/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vascular endothelial growth factor receptor 3 - by Bioz Stars, 2020-09
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    85
    Santa Cruz Biotechnology rabbit anti human flt 4
    <t>VEGFR-3</t> expression on cells in the periphery of the corneal stroma. Confocal micrographs demonstrate VEGFR-3 + dendritic shaped cellular structures in the periphery ( right ) of the cornea, while the central areas ( left ) do not demonstrate such cells ( A ). Higher magnification, confirming the dendritic morphology of VEGFR-3 + cells in the cornea ( B ). Magnification, ( A ) ×200, ( B ) ×1000.
    Rabbit Anti Human Flt 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology vegfr 3
    Lymphatic markers in the adult mouse heart (A–D) Prox-1, <t>VEGFR-3,</t> LYVE-1, and podoplanin were expressed in the lymphatic vessels (arrows) at the atrio-ventricular junction of the adult mouse heart (frontal sections). (E–H) All four lymphatic markers were also expressed in the epicardial lymphatic vessels covering the ventricles (arrows). (I–L) Prox-1 and podoplanin were not expressed in the VEGFR-3-positive/LYVE-1-positive cells and vessels (arrows) found within the ventricular myocardium. V = ventricle. Bars = 100 um.
    Vegfr 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti human vegfr 3 antibody
    Photomicrograph showing immunohistochemical staining of <t>VEGFR-3</t> in cells of prostate cancer tissue. Arrows indicate high intratumoral microlymphatic density (EnVisionTM, magnification 400×).
    Anti Human Vegfr 3 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of pioglitazone on the expression of F4/80 and VEGFR3  in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.

    Journal: Molecular Medicine Reports

    Article Title: Pioglitazone increases VEGFR3 expression and promotes activation of M2 macrophages via the peroxisome proliferator-activated receptor γ

    doi: 10.3892/mmr.2019.9945

    Figure Lengend Snippet: Effect of pioglitazone on the expression of F4/80 and VEGFR3 in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.

    Article Snippet: Then they were incubated overnight at 4°C with rabbit anti-arginase1 (Arg1; cat. no. sc-20150; 1:400; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit anti-vascular endothelial growth factor receptor 3 (VEGFR3; cat. no. sc-321; 1:400; Santa Cruz Biotechnology, Inc.), mouse anti-inducible nitric oxide synthase (iNOS; cat. no. sc-7271; 1:200; Santa Cruz Biotechnology, Inc.) and rabbit anti-α-tubulin (1:5,000; cat. no. ab18251; Abcam, Cambridge, MA, USA).

    Techniques: Expressing, In Vivo, Immunofluorescence, Mouse Assay

    VEGFR-3 expression on cells in the periphery of the corneal stroma. Confocal micrographs demonstrate VEGFR-3 + dendritic shaped cellular structures in the periphery ( right ) of the cornea, while the central areas ( left ) do not demonstrate such cells ( A ). Higher magnification, confirming the dendritic morphology of VEGFR-3 + cells in the cornea ( B ). Magnification, ( A ) ×200, ( B ) ×1000.

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: VEGFR-3 expression on cells in the periphery of the corneal stroma. Confocal micrographs demonstrate VEGFR-3 + dendritic shaped cellular structures in the periphery ( right ) of the cornea, while the central areas ( left ) do not demonstrate such cells ( A ). Higher magnification, confirming the dendritic morphology of VEGFR-3 + cells in the cornea ( B ). Magnification, ( A ) ×200, ( B ) ×1000.

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Expressing

    Immature VEGFR-3 + DC are present throughout the inflamed cornea. Stacked optical sections of the anterior stroma show that the majority of VEGFR-3 + DC (red) do not express MHC class II (green) in normal corneas, and are exclusively located in the peripheral cornea ( A ). During inflammation, VEGFR-3 + DC (red) are also present in the corneal center as early as 72 hours after induction of inflammation, but are mostly negative for MHC class II (green) expression ( B ). Corneas double-stained with VEGFR-3 (red) and CD80 (green) show that VEGFR-3 + cells are mostly CD80 − ( C ). After induction of inflammation, large numbers of VEGFR-3 + cells are also present in the corneal center (red), but generally do not co-express the maturation marker CD80 (green) ( D ). Double staining with CD11c (red) and VEGFR-3 (green) in inflamed corneas shows that the VEGFR-3 expression is present both in the periphery ( upper right ) and the center ( lower left ) of the cornea and that most DC now express VEGFR-3 (yellow) ( E ). Double-staining of human eye bank corneal sections with VEGFR-3 (red) and HLA-DR (green) confirms the presence of VEGFR-3 on stromal cells in the human cornea ( F ). Magnification, ( A , B , E ) ×160, ( C , D , F ) ×400.

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: Immature VEGFR-3 + DC are present throughout the inflamed cornea. Stacked optical sections of the anterior stroma show that the majority of VEGFR-3 + DC (red) do not express MHC class II (green) in normal corneas, and are exclusively located in the peripheral cornea ( A ). During inflammation, VEGFR-3 + DC (red) are also present in the corneal center as early as 72 hours after induction of inflammation, but are mostly negative for MHC class II (green) expression ( B ). Corneas double-stained with VEGFR-3 (red) and CD80 (green) show that VEGFR-3 + cells are mostly CD80 − ( C ). After induction of inflammation, large numbers of VEGFR-3 + cells are also present in the corneal center (red), but generally do not co-express the maturation marker CD80 (green) ( D ). Double staining with CD11c (red) and VEGFR-3 (green) in inflamed corneas shows that the VEGFR-3 expression is present both in the periphery ( upper right ) and the center ( lower left ) of the cornea and that most DC now express VEGFR-3 (yellow) ( E ). Double-staining of human eye bank corneal sections with VEGFR-3 (red) and HLA-DR (green) confirms the presence of VEGFR-3 on stromal cells in the human cornea ( F ). Magnification, ( A , B , E ) ×160, ( C , D , F ) ×400.

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Expressing, Staining, Marker, Double Staining

    VEGFR-3 expression is limited to lymphatic endothelium in normal and inflamed skin and absent on skin DC. To determine whether VEGFR-3 is also expressed on DC in the skin, we examined normal skin tissue sections that were double-stained for VEGFR-3 (red) and LYVE-1 (green) ( A ). VEGFR-3 was present and co-stained with LYVE-1 on lymphatic vessels (yellow), but was not expressed by leukocytes, including DC. Similar stainings with VEGFR-3 (red) and LYVE-1 (green) in inflamed skin ( B ) did not induce novel expression of VEGFR-3 on DC. The expression of VEGFR-3 and LYVE-1 was limited to lymphatic endothelium (yellow) that co-stained for both antibodies. Magnification, ( A and B ) ×200 (Epidermis is on the top ).

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: VEGFR-3 expression is limited to lymphatic endothelium in normal and inflamed skin and absent on skin DC. To determine whether VEGFR-3 is also expressed on DC in the skin, we examined normal skin tissue sections that were double-stained for VEGFR-3 (red) and LYVE-1 (green) ( A ). VEGFR-3 was present and co-stained with LYVE-1 on lymphatic vessels (yellow), but was not expressed by leukocytes, including DC. Similar stainings with VEGFR-3 (red) and LYVE-1 (green) in inflamed skin ( B ) did not induce novel expression of VEGFR-3 on DC. The expression of VEGFR-3 and LYVE-1 was limited to lymphatic endothelium (yellow) that co-stained for both antibodies. Magnification, ( A and B ) ×200 (Epidermis is on the top ).

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Expressing, Staining

    Myeloid dendritic cells in the corneal stroma express VEGFR-3. Whole-mounted corneas were double-stained with CD45 (green) and VEGFR-3 (red). Confocal micrograph of the anterior stroma shows VEGFR-3 + dendritic cells being CD45 + (yellow) ( A ). CD11c (red) expression of these VEGFR-3 + (green) cells provides evidence that they are dendritic cells, and that VEGFR-3 expression is limited to the periphery of the cornea ( right ), while corneal DC in central areas (red) are VEGFR-3 − ( lower left ) ( B ). Additionally, CD11b expression (green) of VEGFR-3 + cells indicates the monocytic lineage of these cells ( C ). Magnification, ( A and C ) ×400. ( B ) ×160.

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: Myeloid dendritic cells in the corneal stroma express VEGFR-3. Whole-mounted corneas were double-stained with CD45 (green) and VEGFR-3 (red). Confocal micrograph of the anterior stroma shows VEGFR-3 + dendritic cells being CD45 + (yellow) ( A ). CD11c (red) expression of these VEGFR-3 + (green) cells provides evidence that they are dendritic cells, and that VEGFR-3 expression is limited to the periphery of the cornea ( right ), while corneal DC in central areas (red) are VEGFR-3 − ( lower left ) ( B ). Additionally, CD11b expression (green) of VEGFR-3 + cells indicates the monocytic lineage of these cells ( C ). Magnification, ( A and C ) ×400. ( B ) ×160.

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Staining, Expressing

    VEGFR-3 expression by dendritiform cells in the corneal stroma. Micrograph of double-stained cornea staining VEGFR-3 (red) and YOYO-1 (green) expression. Significant numbers of dendritic VEGFR-3 + cells ( arrows ) are present in the corneal stroma; these cells are nucleated (yellow) and are present amid VEGFR-3 − cells (green). Magnification, ×400.

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: VEGFR-3 expression by dendritiform cells in the corneal stroma. Micrograph of double-stained cornea staining VEGFR-3 (red) and YOYO-1 (green) expression. Significant numbers of dendritic VEGFR-3 + cells ( arrows ) are present in the corneal stroma; these cells are nucleated (yellow) and are present amid VEGFR-3 − cells (green). Magnification, ×400.

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Expressing, Staining

    VEGFR-3 + DC uniformly co-express VEGF-C during inflammation. Staining of inflamed corneal tissue with VEGFR-3 (green) and VEGF-C (red) demonstrates that VEGFR-3 + DC uniformly co-express the ligand VEGF-C both in the periphery ( A ) and the center ( B ) of corneas. Magnification, ( A ) ×160, ( B ) ×400.

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: VEGFR-3 + DC uniformly co-express VEGF-C during inflammation. Staining of inflamed corneal tissue with VEGFR-3 (green) and VEGF-C (red) demonstrates that VEGFR-3 + DC uniformly co-express the ligand VEGF-C both in the periphery ( A ) and the center ( B ) of corneas. Magnification, ( A ) ×160, ( B ) ×400.

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Staining

    VEGFR-3 staining is intracellular in uninflamed corneas, but membranous in inflamed corneas. To localize the cellular expression of VEGFR-3, we stained unfixed whole-mount corneas with VEGFR-3. Unfixed tissue failed to express VEGFR-3 ( A ). Similar staining of acetone-fixed corneal whole-mounts, however, showed granular cytoplasmic staining as shown with serial confocal sections of a single cell ( B ). Staining of unfixed inflamed corneas demonstrated VEGFR-3 expression on the cell surface ( C ). Magnification, ( A–C ) ×1000.

    Journal: The American Journal of Pathology

    Article Title: Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

    doi:

    Figure Lengend Snippet: VEGFR-3 staining is intracellular in uninflamed corneas, but membranous in inflamed corneas. To localize the cellular expression of VEGFR-3, we stained unfixed whole-mount corneas with VEGFR-3. Unfixed tissue failed to express VEGFR-3 ( A ). Similar staining of acetone-fixed corneal whole-mounts, however, showed granular cytoplasmic staining as shown with serial confocal sections of a single cell ( B ). Staining of unfixed inflamed corneas demonstrated VEGFR-3 expression on the cell surface ( C ). Magnification, ( A–C ) ×1000.

    Article Snippet: The following antibodies (Abs) were used: purified rabbit anti-mouse FLT-4 (VEGFR-3), purified rabbit anti-human FLT-4, purified goat anti-mouse VEGF-C and purified rabbit anti-mouse neuropilin-2 (Santa Cruz Biotechnology, Santa Cruz, CA); purified rat anti-mouse VEGFR-3 (a kind gift from Dr. Hajime Kubo, University of Helsinki, Helsinki, Finland); FITC-conjugated rat anti-mouse DEC-205 (Cedarlane Laboratories Limited, Ontario, Canada); purified rabbit anti-mouse LYVE-1 (a kind gift from Dr. David G. Jackson, Institute of Molecular Medicine Oxford, Oxford, UK); FITC-conjugated hamster anti-mouse CD3 (T cell marker), FITC-conjugated rat anti-mouse CD14 (immature myeloid marker), FITC-conjugated rat anti-mouse CD11b (monocyte/macrophage marker); purified (immunohistochemistry) and PE-conjugated (flow cytometry) hamster anti-mouse CD11c (dendritic cell marker); purified rat anti-mouse CD45 (leukocyte common marker); FITC-conjugated hamster anti-mouse CD80 (costimulatory molecule; B7–1); PE-conjugated rat anti-mouse CD86 (costimulatory molecule; B7–2); FITC-conjugated rat anti-mouse GR-1 (neutrophil marker); FITC-conjugated rat anti-mouse CD8α (lymphoid DC marker); FITC-conjugated mouse anti-mouse IAd (major histocompatibility (MHC) class II) and FITC-conjugated mouse anti-human HLA-DR, DP, DQ.

    Techniques: Staining, Expressing

    Lymphatic markers in the adult mouse heart (A–D) Prox-1, VEGFR-3, LYVE-1, and podoplanin were expressed in the lymphatic vessels (arrows) at the atrio-ventricular junction of the adult mouse heart (frontal sections). (E–H) All four lymphatic markers were also expressed in the epicardial lymphatic vessels covering the ventricles (arrows). (I–L) Prox-1 and podoplanin were not expressed in the VEGFR-3-positive/LYVE-1-positive cells and vessels (arrows) found within the ventricular myocardium. V = ventricle. Bars = 100 um.

    Journal: Anatomical record (Hoboken, N.J. : 2007)

    Article Title: Expression of lymphatic markers during avian and mouse cardiogenesis

    doi: 10.1002/ar.21043

    Figure Lengend Snippet: Lymphatic markers in the adult mouse heart (A–D) Prox-1, VEGFR-3, LYVE-1, and podoplanin were expressed in the lymphatic vessels (arrows) at the atrio-ventricular junction of the adult mouse heart (frontal sections). (E–H) All four lymphatic markers were also expressed in the epicardial lymphatic vessels covering the ventricles (arrows). (I–L) Prox-1 and podoplanin were not expressed in the VEGFR-3-positive/LYVE-1-positive cells and vessels (arrows) found within the ventricular myocardium. V = ventricle. Bars = 100 um.

    Article Snippet: The antibodies against Prox-1 (polyclonal rabbit anti-human, 5 ug/ml; Research Diagnostics Inc., Concord, MA), LYVE-1 (polyclonal rabbit anti-mouse, 10 ug/ml; Angio-Proteomie, Boston, MA), VEGFR-3 (polyclonal rabbit anti-mouse, 4 ug/ml; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), podoplanin (monoclonal hamster anti-mouse, 5 ug/ml, eBioscience, San Diego, CA), CD44 (monoclonal mouse anti-chicken, 125 ug/ml; US Biological Inc., Swampscott, MA), and CD31/PECAM (rat anti-mouse, 10 ug/ml; BP Pharmingen, San Jose, CA) were used according to the manufacturer’s protocol.

    Techniques:

    Lymphatic markers in the ED 9.5 mouse heart (A) Prox-1 was found in cardiac myocytes in the ventricle while LYVE-1 (B) and VEGFR-3 (C) were expressed in cells/vessels on the endocardial side of the myocardium at the leading edge of the nascent interventricular septum. (D) is a high magnification view of the Prox-1-positive cardiac myocytes in the boxed region in (A) . Sections in A–C were 7 microns thick and immediately adjacent to each other. The LYVE-1-positive cells/vessels did not co-localize with the cardiac myocyte marker MF20 (E, F) or with the lymphatic marker podoplanin (G, H) , but they did stain for the endothelial cell marker CD31/PECAM (I–K) . The overlay image (K) for the LYVE-1 and CD31 staining revealed that only a subset of the CD31-positive cells were LYVE-1-positive. Double-positive cells are yellow. Bars = 100 um.

    Journal: Anatomical record (Hoboken, N.J. : 2007)

    Article Title: Expression of lymphatic markers during avian and mouse cardiogenesis

    doi: 10.1002/ar.21043

    Figure Lengend Snippet: Lymphatic markers in the ED 9.5 mouse heart (A) Prox-1 was found in cardiac myocytes in the ventricle while LYVE-1 (B) and VEGFR-3 (C) were expressed in cells/vessels on the endocardial side of the myocardium at the leading edge of the nascent interventricular septum. (D) is a high magnification view of the Prox-1-positive cardiac myocytes in the boxed region in (A) . Sections in A–C were 7 microns thick and immediately adjacent to each other. The LYVE-1-positive cells/vessels did not co-localize with the cardiac myocyte marker MF20 (E, F) or with the lymphatic marker podoplanin (G, H) , but they did stain for the endothelial cell marker CD31/PECAM (I–K) . The overlay image (K) for the LYVE-1 and CD31 staining revealed that only a subset of the CD31-positive cells were LYVE-1-positive. Double-positive cells are yellow. Bars = 100 um.

    Article Snippet: The antibodies against Prox-1 (polyclonal rabbit anti-human, 5 ug/ml; Research Diagnostics Inc., Concord, MA), LYVE-1 (polyclonal rabbit anti-mouse, 10 ug/ml; Angio-Proteomie, Boston, MA), VEGFR-3 (polyclonal rabbit anti-mouse, 4 ug/ml; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), podoplanin (monoclonal hamster anti-mouse, 5 ug/ml, eBioscience, San Diego, CA), CD44 (monoclonal mouse anti-chicken, 125 ug/ml; US Biological Inc., Swampscott, MA), and CD31/PECAM (rat anti-mouse, 10 ug/ml; BP Pharmingen, San Jose, CA) were used according to the manufacturer’s protocol.

    Techniques: Marker, Staining

    Lymphatic markers in vitro (A) Prox-1 expression assessed by immunostaining in quail epicardial cells (QECs) which were also labeled for QH-1 (B) and mounted with DAPI for nuclear visualization (C). (A–C) are the same field of view. (D–F) Expression of lymphatic markers (green) in the adult rat epicardial cells (ARECs). Inserts in (E, F) are confocal images of VEGFR-3 and LYVE-1-expression in the ARECs. (G–I) Expression of lymphatic markers (red) in the embryonic mouse epicardial cells (EMECs). DAPI = blue staining. Bar = 100 um.

    Journal: Anatomical record (Hoboken, N.J. : 2007)

    Article Title: Expression of lymphatic markers during avian and mouse cardiogenesis

    doi: 10.1002/ar.21043

    Figure Lengend Snippet: Lymphatic markers in vitro (A) Prox-1 expression assessed by immunostaining in quail epicardial cells (QECs) which were also labeled for QH-1 (B) and mounted with DAPI for nuclear visualization (C). (A–C) are the same field of view. (D–F) Expression of lymphatic markers (green) in the adult rat epicardial cells (ARECs). Inserts in (E, F) are confocal images of VEGFR-3 and LYVE-1-expression in the ARECs. (G–I) Expression of lymphatic markers (red) in the embryonic mouse epicardial cells (EMECs). DAPI = blue staining. Bar = 100 um.

    Article Snippet: The antibodies against Prox-1 (polyclonal rabbit anti-human, 5 ug/ml; Research Diagnostics Inc., Concord, MA), LYVE-1 (polyclonal rabbit anti-mouse, 10 ug/ml; Angio-Proteomie, Boston, MA), VEGFR-3 (polyclonal rabbit anti-mouse, 4 ug/ml; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), podoplanin (monoclonal hamster anti-mouse, 5 ug/ml, eBioscience, San Diego, CA), CD44 (monoclonal mouse anti-chicken, 125 ug/ml; US Biological Inc., Swampscott, MA), and CD31/PECAM (rat anti-mouse, 10 ug/ml; BP Pharmingen, San Jose, CA) were used according to the manufacturer’s protocol.

    Techniques: In Vitro, Expressing, Immunostaining, Labeling, Staining

    Photomicrograph showing immunohistochemical staining of VEGFR-3 in cells of prostate cancer tissue. Arrows indicate high intratumoral microlymphatic density (EnVisionTM, magnification 400×).

    Journal: Molecules

    Article Title: Associations of nm23H1, VEGF-C, and VEGF-3 Receptor in Human Prostate Cancer

    doi: 10.3390/molecules19056851

    Figure Lengend Snippet: Photomicrograph showing immunohistochemical staining of VEGFR-3 in cells of prostate cancer tissue. Arrows indicate high intratumoral microlymphatic density (EnVisionTM, magnification 400×).

    Article Snippet: After blocked with 10% normal sheep serum for 30 min, the sections were first incubated with anti nm23H1 antibody (GA009601, DAKO, Carpinteria, CA, USA) and anti-human VEGFR-3 antibody (rabbit polyclonal, 1:100 diluted, SC-321, Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4 °C, and then incubated in the Chem MateTM Dako EnVisionTM Detection kit (DakoCytomation A/S, Glostrup, Denmark) at room temperature for 1h.

    Techniques: Immunohistochemistry, Staining