Structured Review

Proteintech rabbit anti upf1
Rabbit Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti upf1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti upf1 - by Bioz Stars, 2024-05
86/100 stars

Images


Structured Review

Proteintech rabbit polyclonal anti upf1
BC cell migration and proliferation are regulated by PVT1 and <t>UPF1.</t> A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
Rabbit Polyclonal Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti upf1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti upf1 - by Bioz Stars, 2024-05
93/100 stars

Images

1) Product Images from "Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1"

Article Title: Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1

Journal: Breast Cancer Research : BCR

doi: 10.1186/s13058-021-01491-y

BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
Figure Legend Snippet: BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

Techniques Used: Migration, Western Blot, Transfection


Structured Review

Proteintech rabbit polyclonal anti upf1
Rabbit Polyclonal Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti upf1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti upf1 - by Bioz Stars, 2024-05
93/100 stars

Images


Structured Review

Proteintech rabbit anti upf1
(A) Representative images of Drosophila eyes expressing the noted <t>UPF1</t> expression modifiers in photoreceptors using a GMR driver. Top images are from flies expressing the noted alleles only, and bottom images are from flies expressing the noted alleles in the context of 30 GGGGCC repeats under the same driver. (B) Violin plots of eye degeneration scores of 30R flies expressing the noted UPF1 alleles. See details for details on scoring method. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. ****p < 0.0001. From left to right, n = 108, 54, 101, 15, 60, 15. Data are represented as violin plots with indicated quartiles. (C) Representative fields of view of propidium-iodide-positive iPSNs in the absence (top row) or presence (bottom row) of 10 μM glutamate. Scale bar, 100 μm. (D) Quantification of the relative proportion of propidium-iodide-positive (dead) cells to total cells from images in (C), represented as “% Cell Death.” n = 3 pairs of age- and sex-matched control and C9orf72 iPSNs, 2 replicates per pair. Six fields of view were analyzed for each data point. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. *p < 0.05, ****p < 0.0001. Data are indicated as mean ± SD. See also .
Rabbit Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti upf1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti upf1 - by Bioz Stars, 2024-05
93/100 stars

Images

1) Product Images from "UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction"

Article Title: UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction

Journal: Cell reports

doi: 10.1016/j.celrep.2021.108925

(A) Representative images of Drosophila eyes expressing the noted UPF1 expression modifiers in photoreceptors using a GMR driver. Top images are from flies expressing the noted alleles only, and bottom images are from flies expressing the noted alleles in the context of 30 GGGGCC repeats under the same driver. (B) Violin plots of eye degeneration scores of 30R flies expressing the noted UPF1 alleles. See details for details on scoring method. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. ****p < 0.0001. From left to right, n = 108, 54, 101, 15, 60, 15. Data are represented as violin plots with indicated quartiles. (C) Representative fields of view of propidium-iodide-positive iPSNs in the absence (top row) or presence (bottom row) of 10 μM glutamate. Scale bar, 100 μm. (D) Quantification of the relative proportion of propidium-iodide-positive (dead) cells to total cells from images in (C), represented as “% Cell Death.” n = 3 pairs of age- and sex-matched control and C9orf72 iPSNs, 2 replicates per pair. Six fields of view were analyzed for each data point. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. *p < 0.05, ****p < 0.0001. Data are indicated as mean ± SD. See also .
Figure Legend Snippet: (A) Representative images of Drosophila eyes expressing the noted UPF1 expression modifiers in photoreceptors using a GMR driver. Top images are from flies expressing the noted alleles only, and bottom images are from flies expressing the noted alleles in the context of 30 GGGGCC repeats under the same driver. (B) Violin plots of eye degeneration scores of 30R flies expressing the noted UPF1 alleles. See details for details on scoring method. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. ****p < 0.0001. From left to right, n = 108, 54, 101, 15, 60, 15. Data are represented as violin plots with indicated quartiles. (C) Representative fields of view of propidium-iodide-positive iPSNs in the absence (top row) or presence (bottom row) of 10 μM glutamate. Scale bar, 100 μm. (D) Quantification of the relative proportion of propidium-iodide-positive (dead) cells to total cells from images in (C), represented as “% Cell Death.” n = 3 pairs of age- and sex-matched control and C9orf72 iPSNs, 2 replicates per pair. Six fields of view were analyzed for each data point. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. *p < 0.05, ****p < 0.0001. Data are indicated as mean ± SD. See also .

Techniques Used: Expressing

(A) Relative abundance of sense repeat RNA following treatment with 0.5 μM SMG1i for the indicated periods of time (24 h 0.1% DMSO treatment used for normalization). n = 4 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. **p < 0.01. (B) Western blot against total UPF1 protein in samples following anti-UPF1 IP from C9-ALS iPSN lysates. Short and long exposures are shown on the top and bottom, respectively. (C) Fold enrichment of sense repeat RNA and ATF4 mRNA relative to GAPDH in IP fraction following anti-UPF1 pulldown as measured by qRT-PCR (input used for normalization). n = 5 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. Data are indicated as mean ± SD. See also .
Figure Legend Snippet: (A) Relative abundance of sense repeat RNA following treatment with 0.5 μM SMG1i for the indicated periods of time (24 h 0.1% DMSO treatment used for normalization). n = 4 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. **p < 0.01. (B) Western blot against total UPF1 protein in samples following anti-UPF1 IP from C9-ALS iPSN lysates. Short and long exposures are shown on the top and bottom, respectively. (C) Fold enrichment of sense repeat RNA and ATF4 mRNA relative to GAPDH in IP fraction following anti-UPF1 pulldown as measured by qRT-PCR (input used for normalization). n = 5 C9-ALS iPSN lines. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. Data are indicated as mean ± SD. See also .

Techniques Used: Western Blot, Quantitative RT-PCR

(A) Ratio of Nluc to Fluc from HeLa cells stably expressing dual-luciferase reporters (with reading frame noted) and transfected control or UPF1 siRNA. n = 3 biological replicates. Unpaired t tests were used to calculate statistical significance. **p < 0.01, ***p < 0.001. (B) Poly(GP) response in control (left) and C9-ALS (right) iPSNs following OE of GFP or UPF1 as measured by an ELISA assay. n = 3 age- and sex-matched pairs of control and C9orf72 iPSNs, 2 replicates each line. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. (C) Relative abundance of sense repeat RNA in C9-ALS iPSNs OE GFP or UPF1. n = 3 C9-ALS iPSN lines, 2 replicates for each line. Paired t tests were used to calculate statistical significance. Data are indicated as mean ± SD. See also .
Figure Legend Snippet: (A) Ratio of Nluc to Fluc from HeLa cells stably expressing dual-luciferase reporters (with reading frame noted) and transfected control or UPF1 siRNA. n = 3 biological replicates. Unpaired t tests were used to calculate statistical significance. **p < 0.01, ***p < 0.001. (B) Poly(GP) response in control (left) and C9-ALS (right) iPSNs following OE of GFP or UPF1 as measured by an ELISA assay. n = 3 age- and sex-matched pairs of control and C9orf72 iPSNs, 2 replicates each line. Ordinary one-way ANOVA was used to calculate statistical significance. *p < 0.05. (C) Relative abundance of sense repeat RNA in C9-ALS iPSNs OE GFP or UPF1. n = 3 C9-ALS iPSN lines, 2 replicates for each line. Paired t tests were used to calculate statistical significance. Data are indicated as mean ± SD. See also .

Techniques Used: Stable Transfection, Expressing, Luciferase, Transfection, Enzyme-linked Immunosorbent Assay

KEY RESOURCES TABLE
Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Clone Assay, Luciferase, Sequencing, Plasmid Preparation, Software, Imaging, Real-time Polymerase Chain Reaction

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    Proteintech rabbit anti upf1
    Rabbit Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti upf1/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti upf1 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    93
    Proteintech rabbit polyclonal anti upf1
    BC cell migration and proliferation are regulated by PVT1 and <t>UPF1.</t> A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
    Rabbit Polyclonal Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti upf1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti upf1 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

    Journal: Breast Cancer Research : BCR

    Article Title: Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1

    doi: 10.1186/s13058-021-01491-y

    Figure Lengend Snippet: BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

    Article Snippet: The primary antibodies were: rabbit monoclonal anti-E-cadherin (#3195, 1:1000, Cell Signaling Technology), rabbit monoclonal anti-Vimentin (#5741, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-UPF1 (23379-1-AP, 1:1000, ProteinTech), rabbit polyclonal anti-FOXQ1 (23718-1-AP, 1:1000, ProteinTech), mouse monoclonal anti-β-actin (A5316, 1:5000, Sigma-Aldrich), and mouse monoclonal anti-PCNA (sc-25280, 1:1000, Santa Cruz).

    Techniques: Migration, Western Blot, Transfection