rabbit anti tspan9  (Proteintech)

Journal: Platelets

Article Title: Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation

doi: 10.1080/09537104.2016.1254175

Figure Lengend Snippet: Tspan9-deficient mice have platelets of normal count and size, and normal numbers of other blood cell parameters. Whole blood from wild-type (WT) and Tspan9-deficient (KO) mice was analysed using an ABX Pentra 60 haematology analyser from Block Scientific. Data are presented as means and standard deviations from between 18 and 20 litter-matched pairs of mice. No significant differences were revealed using unpaired t-tests.

Article Snippet: The rabbit anti-Tspan9 polyclonal antibody was as previously described [ ], anti-α-tubulin monoclonal DM1A was from Sigma, anti-phosphotyrosine monoclonal 4G10 was from Millipore, anti-LAT phospho-specific antibodies were from Abcam, anti-human GPVI monoclonal HY101 for biochemical experiments was kindly provided by Dr Peter Smethurst (NHS Blood and Transplant, Cambridge, UK), anti-human GPVI monoclonal 1G5 for microscopy was kindly provided by Dr Elizabeth Gardiner (The John Curtin School of Medical Research, Canberra, Australia) [ ] and mouse IgG1 isotype control monoclonal MOPC-21 was from Sigma.

Techniques: Blocking Assay, Cell Counting, Concentration Assay

Journal: Platelets

Article Title: Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation

doi: 10.1080/09537104.2016.1254175

Figure Lengend Snippet: Tspan9-deficient platelets express normal levels of the major platelet surface glycoproteins. Whole blood from wild-type (WT) and Tspan9-deficient (KO) mice were stained with fluorescent-conjugated antibodies, and platelet-specific staining was determined following gating by size. Isotype negative control staining was subtracted, and geometric mean fluorescence intensities with standard deviations are presented from between 3 and 8 pairs of litter-matched mice. No significant differences were determined using unpaired t-tests.

Article Snippet: The rabbit anti-Tspan9 polyclonal antibody was as previously described [ ], anti-α-tubulin monoclonal DM1A was from Sigma, anti-phosphotyrosine monoclonal 4G10 was from Millipore, anti-LAT phospho-specific antibodies were from Abcam, anti-human GPVI monoclonal HY101 for biochemical experiments was kindly provided by Dr Peter Smethurst (NHS Blood and Transplant, Cambridge, UK), anti-human GPVI monoclonal 1G5 for microscopy was kindly provided by Dr Elizabeth Gardiner (The John Curtin School of Medical Research, Canberra, Australia) [ ] and mouse IgG1 isotype control monoclonal MOPC-21 was from Sigma.

Techniques: Staining, Negative Control, Fluorescence