rabbit anti tpcn2 monoclonal antibodies  (Alomone Labs)


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    Alomone Labs rabbit anti tpcn2 monoclonal antibodies
    The primers used in the quantitative reverse transcription polymerase chain reaction.
    Rabbit Anti Tpcn2 Monoclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tpcn2 monoclonal antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti tpcn2 monoclonal antibodies - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle"

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    Journal: Chinese Medical Journal

    doi: 10.1097/CM9.0000000000001893

    The primers used in the quantitative reverse transcription polymerase chain reaction.
    Figure Legend Snippet: The primers used in the quantitative reverse transcription polymerase chain reaction.

    Techniques Used:

    TPCN2 -knockdown inhibits the proliferation in SLE. (A) Expression level of TPCN2 in PBMCs of SLE cases ( n = 6) and healthy controls ( n = 7). ∗ P < 0.05, Student's t test. Knockdown of TPCN2 with two independent shRNA in Jurkat (B) and THP-1 (C) cells; the left panel represents the relative expression levels of TPCN2 assessed by qRT-PCR; the right panel represents protein expression level of TPCN2 that was detected by Western blotting. The data represent the mean ( n = 3) ± SD. ∗ P < 0.05, Student's t test. Cell viability of silencing TPCN2 in Jurkat (D) and THP-1 (E) cells were determined by CCK-8 assay. The results represent the means ( n = 5) ± SD. Significant differences were evaluated using Student's t test. ∗ P < 0.05. CCK-8: Cell count kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; PBMCs: Peripheral blood mononuclear cells; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: Standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: TPCN2 -knockdown inhibits the proliferation in SLE. (A) Expression level of TPCN2 in PBMCs of SLE cases ( n = 6) and healthy controls ( n = 7). ∗ P < 0.05, Student's t test. Knockdown of TPCN2 with two independent shRNA in Jurkat (B) and THP-1 (C) cells; the left panel represents the relative expression levels of TPCN2 assessed by qRT-PCR; the right panel represents protein expression level of TPCN2 that was detected by Western blotting. The data represent the mean ( n = 3) ± SD. ∗ P < 0.05, Student's t test. Cell viability of silencing TPCN2 in Jurkat (D) and THP-1 (E) cells were determined by CCK-8 assay. The results represent the means ( n = 5) ± SD. Significant differences were evaluated using Student's t test. ∗ P < 0.05. CCK-8: Cell count kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; PBMCs: Peripheral blood mononuclear cells; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: Standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Techniques Used: Expressing, shRNA, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Cell Counting, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Knockdown of TPCN2 induces apoptosis and cell-cycle arrest in SLE. Apoptosis was detected by flow cytometry with Annexin V and PI in jurkat- TPCN2 -knockdown cells (A) and THP-1- TPCN2 -knockdown cells (B). TPCN2 -knockdown induced G2/M cell-cycle arrest. The percentage of G2-M phase cells of Jurkat (C) and THP-1 (D) was assessed by flow cytometry. ∗ P < 0.05. NC: Normal control; PI: Propidium iodide; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: Knockdown of TPCN2 induces apoptosis and cell-cycle arrest in SLE. Apoptosis was detected by flow cytometry with Annexin V and PI in jurkat- TPCN2 -knockdown cells (A) and THP-1- TPCN2 -knockdown cells (B). TPCN2 -knockdown induced G2/M cell-cycle arrest. The percentage of G2-M phase cells of Jurkat (C) and THP-1 (D) was assessed by flow cytometry. ∗ P < 0.05. NC: Normal control; PI: Propidium iodide; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Techniques Used: Flow Cytometry

    RNA-seq analyses of the effect of TPCN2 -knockdown on the gene expression profile. (A) The heatmap shows differential expression genes in sh#1 and sh#2 (in comparison with NC group). (B) The differential statistics of DEGs in NC groups vs . sh#1/sh#2. Blue columnar represent up-regulated DEGs. Red column represents down-regulated DEGs. (C) GO classification analysis of DEGs. NC group vs . sh#2. Molecular function – blue; Cellular components – red; Biological process – green. (D) Top 20 enriched KEGG pathways after silencing TPCN2 in Jurkat. The x-axis is the enrichment score, and the y-axis is the enriched pathways. DEGs: Differentially expressed genes; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; NC: Normal control; RNA-seq: RNA sequencing; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: RNA-seq analyses of the effect of TPCN2 -knockdown on the gene expression profile. (A) The heatmap shows differential expression genes in sh#1 and sh#2 (in comparison with NC group). (B) The differential statistics of DEGs in NC groups vs . sh#1/sh#2. Blue columnar represent up-regulated DEGs. Red column represents down-regulated DEGs. (C) GO classification analysis of DEGs. NC group vs . sh#2. Molecular function – blue; Cellular components – red; Biological process – green. (D) Top 20 enriched KEGG pathways after silencing TPCN2 in Jurkat. The x-axis is the enrichment score, and the y-axis is the enriched pathways. DEGs: Differentially expressed genes; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; NC: Normal control; RNA-seq: RNA sequencing; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; TPCN2 : Two-pore segment channel 2.

    Techniques Used: RNA Sequencing Assay, Expressing

    Representative enriched pathways in high-risk shTPCN2#2 through GSEA analysis. GSEA results showed that the G2/M checkpoint (A), inflammatory response (B), complement (C), and PI3K-AKT-mTOR (D) pathways were enriched in the sh#2 expression group. Top panels indicate the enrichment scores for each gene. Bottom panels show the ranking metrics of each gene. Y-axis: ranking metric values; X-axis: ranks for all genes. (E) The mRNA expression of some DEGs in Jurkat cells were detected by qRT-PCR. The RNA was extracted from cells knocked down of TPCN2 with two independent shRNA. The results were shown as the mean ± SD from three independent experiments. ∗ P < 0.05. AKT: Protein kinase B; DEGs: Differentially expressed genes; GSEA: Gene set enrichment analysis; mTOR: Mechanistic target of rapamycin; NC: Normal control; NES: Normalized enrichment score; PI3K: Phosphatidylinositol 3-kinase; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: Representative enriched pathways in high-risk shTPCN2#2 through GSEA analysis. GSEA results showed that the G2/M checkpoint (A), inflammatory response (B), complement (C), and PI3K-AKT-mTOR (D) pathways were enriched in the sh#2 expression group. Top panels indicate the enrichment scores for each gene. Bottom panels show the ranking metrics of each gene. Y-axis: ranking metric values; X-axis: ranks for all genes. (E) The mRNA expression of some DEGs in Jurkat cells were detected by qRT-PCR. The RNA was extracted from cells knocked down of TPCN2 with two independent shRNA. The results were shown as the mean ± SD from three independent experiments. ∗ P < 0.05. AKT: Protein kinase B; DEGs: Differentially expressed genes; GSEA: Gene set enrichment analysis; mTOR: Mechanistic target of rapamycin; NC: Normal control; NES: Normalized enrichment score; PI3K: Phosphatidylinositol 3-kinase; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; TPCN2 : Two-pore segment channel 2.

    Techniques Used: Expressing, Quantitative RT-PCR, shRNA, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

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    Alomone Labs rabbit anti tpcn2 monoclonal antibodies
    The primers used in the quantitative reverse transcription polymerase chain reaction.
    Rabbit Anti Tpcn2 Monoclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tpcn2 monoclonal antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti tpcn2 monoclonal antibodies - by Bioz Stars, 2023-02
    94/100 stars
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    The primers used in the quantitative reverse transcription polymerase chain reaction.

    Journal: Chinese Medical Journal

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    doi: 10.1097/CM9.0000000000001893

    Figure Lengend Snippet: The primers used in the quantitative reverse transcription polymerase chain reaction.

    Article Snippet: The membranes were blocked with 5% nonfat dry milk for 1 h, and then incubated with rabbit anti- TPCN2 monoclonal antibodies (1:200; Alomone Labs, Jerusalem, Israel) overnight at 4°C.

    Techniques:

    TPCN2 -knockdown inhibits the proliferation in SLE. (A) Expression level of TPCN2 in PBMCs of SLE cases ( n = 6) and healthy controls ( n = 7). ∗ P < 0.05, Student's t test. Knockdown of TPCN2 with two independent shRNA in Jurkat (B) and THP-1 (C) cells; the left panel represents the relative expression levels of TPCN2 assessed by qRT-PCR; the right panel represents protein expression level of TPCN2 that was detected by Western blotting. The data represent the mean ( n = 3) ± SD. ∗ P < 0.05, Student's t test. Cell viability of silencing TPCN2 in Jurkat (D) and THP-1 (E) cells were determined by CCK-8 assay. The results represent the means ( n = 5) ± SD. Significant differences were evaluated using Student's t test. ∗ P < 0.05. CCK-8: Cell count kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; PBMCs: Peripheral blood mononuclear cells; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: Standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Journal: Chinese Medical Journal

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    doi: 10.1097/CM9.0000000000001893

    Figure Lengend Snippet: TPCN2 -knockdown inhibits the proliferation in SLE. (A) Expression level of TPCN2 in PBMCs of SLE cases ( n = 6) and healthy controls ( n = 7). ∗ P < 0.05, Student's t test. Knockdown of TPCN2 with two independent shRNA in Jurkat (B) and THP-1 (C) cells; the left panel represents the relative expression levels of TPCN2 assessed by qRT-PCR; the right panel represents protein expression level of TPCN2 that was detected by Western blotting. The data represent the mean ( n = 3) ± SD. ∗ P < 0.05, Student's t test. Cell viability of silencing TPCN2 in Jurkat (D) and THP-1 (E) cells were determined by CCK-8 assay. The results represent the means ( n = 5) ± SD. Significant differences were evaluated using Student's t test. ∗ P < 0.05. CCK-8: Cell count kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; PBMCs: Peripheral blood mononuclear cells; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: Standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Article Snippet: The membranes were blocked with 5% nonfat dry milk for 1 h, and then incubated with rabbit anti- TPCN2 monoclonal antibodies (1:200; Alomone Labs, Jerusalem, Israel) overnight at 4°C.

    Techniques: Expressing, shRNA, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Cell Counting, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Knockdown of TPCN2 induces apoptosis and cell-cycle arrest in SLE. Apoptosis was detected by flow cytometry with Annexin V and PI in jurkat- TPCN2 -knockdown cells (A) and THP-1- TPCN2 -knockdown cells (B). TPCN2 -knockdown induced G2/M cell-cycle arrest. The percentage of G2-M phase cells of Jurkat (C) and THP-1 (D) was assessed by flow cytometry. ∗ P < 0.05. NC: Normal control; PI: Propidium iodide; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Journal: Chinese Medical Journal

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    doi: 10.1097/CM9.0000000000001893

    Figure Lengend Snippet: Knockdown of TPCN2 induces apoptosis and cell-cycle arrest in SLE. Apoptosis was detected by flow cytometry with Annexin V and PI in jurkat- TPCN2 -knockdown cells (A) and THP-1- TPCN2 -knockdown cells (B). TPCN2 -knockdown induced G2/M cell-cycle arrest. The percentage of G2-M phase cells of Jurkat (C) and THP-1 (D) was assessed by flow cytometry. ∗ P < 0.05. NC: Normal control; PI: Propidium iodide; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Article Snippet: The membranes were blocked with 5% nonfat dry milk for 1 h, and then incubated with rabbit anti- TPCN2 monoclonal antibodies (1:200; Alomone Labs, Jerusalem, Israel) overnight at 4°C.

    Techniques: Flow Cytometry

    RNA-seq analyses of the effect of TPCN2 -knockdown on the gene expression profile. (A) The heatmap shows differential expression genes in sh#1 and sh#2 (in comparison with NC group). (B) The differential statistics of DEGs in NC groups vs . sh#1/sh#2. Blue columnar represent up-regulated DEGs. Red column represents down-regulated DEGs. (C) GO classification analysis of DEGs. NC group vs . sh#2. Molecular function – blue; Cellular components – red; Biological process – green. (D) Top 20 enriched KEGG pathways after silencing TPCN2 in Jurkat. The x-axis is the enrichment score, and the y-axis is the enriched pathways. DEGs: Differentially expressed genes; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; NC: Normal control; RNA-seq: RNA sequencing; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; TPCN2 : Two-pore segment channel 2.

    Journal: Chinese Medical Journal

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    doi: 10.1097/CM9.0000000000001893

    Figure Lengend Snippet: RNA-seq analyses of the effect of TPCN2 -knockdown on the gene expression profile. (A) The heatmap shows differential expression genes in sh#1 and sh#2 (in comparison with NC group). (B) The differential statistics of DEGs in NC groups vs . sh#1/sh#2. Blue columnar represent up-regulated DEGs. Red column represents down-regulated DEGs. (C) GO classification analysis of DEGs. NC group vs . sh#2. Molecular function – blue; Cellular components – red; Biological process – green. (D) Top 20 enriched KEGG pathways after silencing TPCN2 in Jurkat. The x-axis is the enrichment score, and the y-axis is the enriched pathways. DEGs: Differentially expressed genes; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; NC: Normal control; RNA-seq: RNA sequencing; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; TPCN2 : Two-pore segment channel 2.

    Article Snippet: The membranes were blocked with 5% nonfat dry milk for 1 h, and then incubated with rabbit anti- TPCN2 monoclonal antibodies (1:200; Alomone Labs, Jerusalem, Israel) overnight at 4°C.

    Techniques: RNA Sequencing Assay, Expressing

    Representative enriched pathways in high-risk shTPCN2#2 through GSEA analysis. GSEA results showed that the G2/M checkpoint (A), inflammatory response (B), complement (C), and PI3K-AKT-mTOR (D) pathways were enriched in the sh#2 expression group. Top panels indicate the enrichment scores for each gene. Bottom panels show the ranking metrics of each gene. Y-axis: ranking metric values; X-axis: ranks for all genes. (E) The mRNA expression of some DEGs in Jurkat cells were detected by qRT-PCR. The RNA was extracted from cells knocked down of TPCN2 with two independent shRNA. The results were shown as the mean ± SD from three independent experiments. ∗ P < 0.05. AKT: Protein kinase B; DEGs: Differentially expressed genes; GSEA: Gene set enrichment analysis; mTOR: Mechanistic target of rapamycin; NC: Normal control; NES: Normalized enrichment score; PI3K: Phosphatidylinositol 3-kinase; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; TPCN2 : Two-pore segment channel 2.

    Journal: Chinese Medical Journal

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    doi: 10.1097/CM9.0000000000001893

    Figure Lengend Snippet: Representative enriched pathways in high-risk shTPCN2#2 through GSEA analysis. GSEA results showed that the G2/M checkpoint (A), inflammatory response (B), complement (C), and PI3K-AKT-mTOR (D) pathways were enriched in the sh#2 expression group. Top panels indicate the enrichment scores for each gene. Bottom panels show the ranking metrics of each gene. Y-axis: ranking metric values; X-axis: ranks for all genes. (E) The mRNA expression of some DEGs in Jurkat cells were detected by qRT-PCR. The RNA was extracted from cells knocked down of TPCN2 with two independent shRNA. The results were shown as the mean ± SD from three independent experiments. ∗ P < 0.05. AKT: Protein kinase B; DEGs: Differentially expressed genes; GSEA: Gene set enrichment analysis; mTOR: Mechanistic target of rapamycin; NC: Normal control; NES: Normalized enrichment score; PI3K: Phosphatidylinositol 3-kinase; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; TPCN2 : Two-pore segment channel 2.

    Article Snippet: The membranes were blocked with 5% nonfat dry milk for 1 h, and then incubated with rabbit anti- TPCN2 monoclonal antibodies (1:200; Alomone Labs, Jerusalem, Israel) overnight at 4°C.

    Techniques: Expressing, Quantitative RT-PCR, shRNA, Reverse Transcription Polymerase Chain Reaction, Standard Deviation