rabbit anti sheep immunoglobulin g  (Valiant)

 
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    Name:
    Anti sheep IgG whole molecule affinity purified rabbit antibody fluorescein conjugated
    Description:
    Product is fluorescein 5 isothiocyanate FITC Isomer I conjugated rabbit affinity purified antibody to sheep IgG whole molecule and buffer salts
    Catalog Number:
    0857005
    Price:
    198.55
    Category:
    Life Sciences Antibodies Secondary Antibodies
    Applications:
    Immunoassays, Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen), Immunofluorescence assays (IFA), Flow Cytometry (FACS)
    Size:
    2 mg
    Buy from Supplier


    Structured Review

    Valiant rabbit anti sheep immunoglobulin g
    Anti sheep IgG whole molecule affinity purified rabbit antibody fluorescein conjugated
    Product is fluorescein 5 isothiocyanate FITC Isomer I conjugated rabbit affinity purified antibody to sheep IgG whole molecule and buffer salts
    https://www.bioz.com/result/rabbit anti sheep immunoglobulin g/product/Valiant
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti sheep immunoglobulin g - by Bioz Stars, 2021-05
    91/100 stars

    Images

    1) Product Images from "Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy"

    Article Title: Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E16-12-0828

    Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.
    Figure Legend Snippet: Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.

    Techniques Used: Mouse Assay, Staining, Immunofluorescence, Fluorescence

    Related Articles

    other:

    Article Title: Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy
    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse complement C3 antibody (Cappel 55500) and rabbit anti-sheep immunoglobulin G (IgG; 0865205) were purchased from MP Biomedicals (Santa Ana, CA).

    Incubation:

    Article Title: Transmembrane pickets connect cyto-and pericellular-skeletons forming barriers to receptor engagement
    Article Snippet: To visualize the IgG, coverslips were stained with 0.1 μg/mL Cy3-conjugated donkey anti-human antibody (Jackson ImmunoResearch) for 5 min. .. For opsonisation of erythrocytes, 0.5 mg/mL of rabbit anti-sheep IgG (MP Biomedicals) was incubated at with 200 μL of a 1% solution of washed sheep erythrocytes (MP Biomedicals) for 2 h at 37 °C. .. For opsonisation, 200 μL of a suspension (1% solids in PBS) of polystyrene beads containing 2% divinylbenzene (1.5, 5, or 8 μm diameter; Bangs Laboratories) was incubated with human IgG (50, 10, or 5 μg/mL; Sigma) for 2 h at 37°C.

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  • 91
    Valiant rabbit anti sheep immunoglobulin g
    Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune <t>IgG</t> is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.
    Rabbit Anti Sheep Immunoglobulin G, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti sheep immunoglobulin g/product/Valiant
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti sheep immunoglobulin g - by Bioz Stars, 2021-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.

    Journal: Molecular Biology of the Cell

    Article Title: Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy

    doi: 10.1091/mbc.E16-12-0828

    Figure Lengend Snippet: Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.

    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse complement C3 antibody (Cappel 55500) and rabbit anti-sheep immunoglobulin G (IgG; 0865205) were purchased from MP Biomedicals (Santa Ana, CA).

    Techniques: Mouse Assay, Staining, Immunofluorescence, Fluorescence