rabbit anti rat  (Bio-Rad)

 
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  • 93
    Name:
    Rabbit anti Rat IgG H L
    Description:

    Catalog Number:
    618501
    Price:
    None
    Applications:
    ELISA
    Format:
    Purified
    Buy from Supplier


    Structured Review

    Bio-Rad rabbit anti rat

    https://www.bioz.com/result/rabbit anti rat/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat - by Bioz Stars, 2020-09
    93/100 stars

    Images

    Related Articles

    Transduction:

    Article Title: Platelet-activating factor increases VE-cadherin tyrosine phosphorylation in mouse endothelial cells and its association with the PtdIns3'-kinase
    Article Snippet: .. Commercially available antibodies used were as follows: for immunoprecipitation, monoclonal antiphosphotyrosine mAb 4G10 (Upstate Biotechnology, Inc., Lake Placid, NY), mouse monoclonal anti-p85 subunit of PtdIns3′-kinase (Transduction Laboratories, Lexington, KY), and for western blotting, monoclonal antiphosphotyrosine mAb 4G10, polyclonal anti-phospho Akt, polyclonal anti-active MAPK (Promega, Madison), and horseradish peroxidase-conjugated goat anti–mouse IgG, goat anti–rabbit IgG, rabbit anti-rat (Bio-Rad Laboratories (Hercules, CA). .. For immunoflorescence, Cy3-conjugated affinipure goat anti-rat IgG and goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, Inc. (Westgrove, PA).

    Electrophoresis:

    Article Title: Effect of Abnormal Savda Munziq, a Traditional Uighur Herbal Medicine, on Pulmonary Function and Aquaporins of COPD Rat Model with Abnormal Savda Syndrome
    Article Snippet: .. The proteins were transferred to a membrane at 100 V for 2 h following electrophoresis at 120 V for 1 h. The membrane was blocked with 5% skim milk in TBST for 1 h, followed by incubation with goat anti-rat monoclonal antibodies against AQP1, AQP4, and AQP5, respectively, at 4°C overnight (all 1 : 200), and detected by rabbit anti-rat IgG secondary antibody (1 : 1000) after incubation at room temperature for 1 h. The membranes were further treated with ECL developer and visualized as well as analyzed by Bio-Rad software for grey scale analysis. .. The relative expression level of AQP1, AQP4, and AQP5 was estimated by normalizing against that of β -actin as the internal control.

    Immunoprecipitation:

    Article Title: Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells
    Article Snippet: .. The lysate was then separated into two portions of same volume and immunoprecipitation was performed with mAb clone 9 (20 μg) and rat serum IgG (20 μg) with 100 μl rabbit anti-rat IgG-conjugated protein A Sepharose bead suspension at 4 °C for 3 h. Immunoprecipitated proteins were resolved on a 12% SDS-PAGE under reducing conditions and electro-transferred onto PVDF membrane (Bio-Rad). .. Western blots were performed using anti-kindlin-3 mAb clone 9 and primary antibodies from different sources following manufacturers’ instructions.

    Article Title: Platelet-activating factor increases VE-cadherin tyrosine phosphorylation in mouse endothelial cells and its association with the PtdIns3'-kinase
    Article Snippet: .. Commercially available antibodies used were as follows: for immunoprecipitation, monoclonal antiphosphotyrosine mAb 4G10 (Upstate Biotechnology, Inc., Lake Placid, NY), mouse monoclonal anti-p85 subunit of PtdIns3′-kinase (Transduction Laboratories, Lexington, KY), and for western blotting, monoclonal antiphosphotyrosine mAb 4G10, polyclonal anti-phospho Akt, polyclonal anti-active MAPK (Promega, Madison), and horseradish peroxidase-conjugated goat anti–mouse IgG, goat anti–rabbit IgG, rabbit anti-rat (Bio-Rad Laboratories (Hercules, CA). .. For immunoflorescence, Cy3-conjugated affinipure goat anti-rat IgG and goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, Inc. (Westgrove, PA).

    Incubation:

    Article Title: Effect of Abnormal Savda Munziq, a Traditional Uighur Herbal Medicine, on Pulmonary Function and Aquaporins of COPD Rat Model with Abnormal Savda Syndrome
    Article Snippet: .. The proteins were transferred to a membrane at 100 V for 2 h following electrophoresis at 120 V for 1 h. The membrane was blocked with 5% skim milk in TBST for 1 h, followed by incubation with goat anti-rat monoclonal antibodies against AQP1, AQP4, and AQP5, respectively, at 4°C overnight (all 1 : 200), and detected by rabbit anti-rat IgG secondary antibody (1 : 1000) after incubation at room temperature for 1 h. The membranes were further treated with ECL developer and visualized as well as analyzed by Bio-Rad software for grey scale analysis. .. The relative expression level of AQP1, AQP4, and AQP5 was estimated by normalizing against that of β -actin as the internal control.

    Western Blot:

    Article Title: Platelet-activating factor increases VE-cadherin tyrosine phosphorylation in mouse endothelial cells and its association with the PtdIns3'-kinase
    Article Snippet: .. Commercially available antibodies used were as follows: for immunoprecipitation, monoclonal antiphosphotyrosine mAb 4G10 (Upstate Biotechnology, Inc., Lake Placid, NY), mouse monoclonal anti-p85 subunit of PtdIns3′-kinase (Transduction Laboratories, Lexington, KY), and for western blotting, monoclonal antiphosphotyrosine mAb 4G10, polyclonal anti-phospho Akt, polyclonal anti-active MAPK (Promega, Madison), and horseradish peroxidase-conjugated goat anti–mouse IgG, goat anti–rabbit IgG, rabbit anti-rat (Bio-Rad Laboratories (Hercules, CA). .. For immunoflorescence, Cy3-conjugated affinipure goat anti-rat IgG and goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, Inc. (Westgrove, PA).

    SDS Page:

    Article Title: Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells
    Article Snippet: .. The lysate was then separated into two portions of same volume and immunoprecipitation was performed with mAb clone 9 (20 μg) and rat serum IgG (20 μg) with 100 μl rabbit anti-rat IgG-conjugated protein A Sepharose bead suspension at 4 °C for 3 h. Immunoprecipitated proteins were resolved on a 12% SDS-PAGE under reducing conditions and electro-transferred onto PVDF membrane (Bio-Rad). .. Western blots were performed using anti-kindlin-3 mAb clone 9 and primary antibodies from different sources following manufacturers’ instructions.

    Software:

    Article Title: Effect of Abnormal Savda Munziq, a Traditional Uighur Herbal Medicine, on Pulmonary Function and Aquaporins of COPD Rat Model with Abnormal Savda Syndrome
    Article Snippet: .. The proteins were transferred to a membrane at 100 V for 2 h following electrophoresis at 120 V for 1 h. The membrane was blocked with 5% skim milk in TBST for 1 h, followed by incubation with goat anti-rat monoclonal antibodies against AQP1, AQP4, and AQP5, respectively, at 4°C overnight (all 1 : 200), and detected by rabbit anti-rat IgG secondary antibody (1 : 1000) after incubation at room temperature for 1 h. The membranes were further treated with ECL developer and visualized as well as analyzed by Bio-Rad software for grey scale analysis. .. The relative expression level of AQP1, AQP4, and AQP5 was estimated by normalizing against that of β -actin as the internal control.

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  • 99
    Bio-Rad monoclonal rat anti f4 80 antibody
    Effect of SAB treatment on adipose and hepatic tissue inflammation in HFD-fed BDNF M/M mice. A and B , representative image of foam cell staining in adipose ( A ) and hepatic ( B ) tissues. Lipids were stained with Oil Red O. C , SAB-treated obese animals showed less optical density (mean intensity) and smaller lipid droplet size. D and E , SAB intervention decreased macrophage accumulation in adipose ( D ) and hepatic ( E ) tissues. F , SAB-treated animals showed decreased CD36 and <t>F4/80</t> level and lower inflammatory gene expressions in adipose tissue. *, p
    Monoclonal Rat Anti F4 80 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rat anti f4 80 antibody/product/Bio-Rad
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    monoclonal rat anti f4 80 antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    95
    Bio-Rad mouse immunoglobulin g
    Rubulavirus SV5 V gene induces Stat1 ubiquitination but not pY701-Stat1 formation. 2fTGH cells were cotransfected with plasmids expressing GFP and Stat1 along with the SeV C , SeV C Δ10-15 , or SV5 V gene or an empty plasmid. Total cell extracts were prepared at 24 h posttransfection, and equal amounts of extract were Western blotted with either anti-Stat1 (bulk, top panel) or anti-pY701-Stat1 plus antiactin (middle panel). Equal amounts of extract were also precipitated with antiubiquitin (Ub) and then Western blotted with anti-Stat1 (bottom panel). The right-hand lane in the bottom panel has not been immunoprecipitated and shows the electrophoretic position of bulk Stat1. <t>IgG-hc,</t> IgG heavy chain.
    Mouse Immunoglobulin G, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse immunoglobulin g/product/Bio-Rad
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse immunoglobulin g - by Bioz Stars, 2020-09
    95/100 stars
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    Image Search Results


    Effect of SAB treatment on adipose and hepatic tissue inflammation in HFD-fed BDNF M/M mice. A and B , representative image of foam cell staining in adipose ( A ) and hepatic ( B ) tissues. Lipids were stained with Oil Red O. C , SAB-treated obese animals showed less optical density (mean intensity) and smaller lipid droplet size. D and E , SAB intervention decreased macrophage accumulation in adipose ( D ) and hepatic ( E ) tissues. F , SAB-treated animals showed decreased CD36 and F4/80 level and lower inflammatory gene expressions in adipose tissue. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Inhibition of the CD36 receptor reduces visceral fat accumulation and improves insulin resistance in obese mice carrying the BDNF-Val66Met variant

    doi: 10.1074/jbc.RA118.002405

    Figure Lengend Snippet: Effect of SAB treatment on adipose and hepatic tissue inflammation in HFD-fed BDNF M/M mice. A and B , representative image of foam cell staining in adipose ( A ) and hepatic ( B ) tissues. Lipids were stained with Oil Red O. C , SAB-treated obese animals showed less optical density (mean intensity) and smaller lipid droplet size. D and E , SAB intervention decreased macrophage accumulation in adipose ( D ) and hepatic ( E ) tissues. F , SAB-treated animals showed decreased CD36 and F4/80 level and lower inflammatory gene expressions in adipose tissue. *, p

    Article Snippet: Immunohistochemical staining for macrophages was performed with monoclonal rat anti-F4/80 antibody (1:100, MCA497GA, lot 1610, Bio-Rad).

    Techniques: Mouse Assay, Staining

    Macrophage infiltration in the heart tissues and plasma levels of proinflammatory cytokines. a Macrophage infiltration into mouse heart tissues at week 12 visualized by F4/80 staining with 20× magnification of the interventricular septum region shown in the lower right corner. Scale bar equals 2 mm and 60 µm. A single representative section for each cohort is shown. b – e Plasma cytokines were determined by electrochemiluminescence assay at week 3: b CCL2; c CXCL10; d CXCL1; and e TNF-α. WT-chow ( n = 10), DKO-chow ( n = 12), WT-PSRD ( n = 9), DKO-PSRD ( n = 22). Data were analyzed using one-way ANOVA and presented using box-and-whisker plot, where the boxes encompass the first to the third quartiles, inside the box the horizontal line shows the median, and the whiskers are the maximum and minimum observation. * p

    Journal: Communications Biology

    Article Title: Stigmasterol accumulation causes cardiac injury and promotes mortality

    doi: 10.1038/s42003-018-0245-x

    Figure Lengend Snippet: Macrophage infiltration in the heart tissues and plasma levels of proinflammatory cytokines. a Macrophage infiltration into mouse heart tissues at week 12 visualized by F4/80 staining with 20× magnification of the interventricular septum region shown in the lower right corner. Scale bar equals 2 mm and 60 µm. A single representative section for each cohort is shown. b – e Plasma cytokines were determined by electrochemiluminescence assay at week 3: b CCL2; c CXCL10; d CXCL1; and e TNF-α. WT-chow ( n = 10), DKO-chow ( n = 12), WT-PSRD ( n = 9), DKO-PSRD ( n = 22). Data were analyzed using one-way ANOVA and presented using box-and-whisker plot, where the boxes encompass the first to the third quartiles, inside the box the horizontal line shows the median, and the whiskers are the maximum and minimum observation. * p

    Article Snippet: Sections were also stained with rat anti-F4/80 antibody (1:2000, BioRad MCA497R/Lot 1014) for 60 min, washed, followed by incubation with secondary antibody (Rat 1-Step Polymer, Biocare BRR4016L).

    Techniques: Staining, Electrochemiluminescence, Whisker Assay

    Macrophage infiltration in the heart tissues and plasma levels of proinflammatory cytokines. a Macrophage infiltration into mouse heart tissues at week 12 visualized by F4/80 staining with 20× magnification of the interventricular septum region shown in the lower right corner. Scale bar equals 2 mm and 60 µm. A single representative section for each cohort is shown. b – e Plasma cytokines were determined by electrochemiluminescence assay at week 3: b CCL2; c CXCL10; d CXCL1; and e TNF-α. WT-chow ( n = 10), DKO-chow ( n = 12), WT-PSRD ( n = 9), DKO-PSRD ( n = 22). Data were analyzed using one-way ANOVA and presented using box-and-whisker plot, where the boxes encompass the first to the third quartiles, inside the box the horizontal line shows the median, and the whiskers are the maximum and minimum observation. * p

    Journal: Communications Biology

    Article Title: Stigmasterol accumulation causes cardiac injury and promotes mortality

    doi: 10.1038/s42003-018-0245-x

    Figure Lengend Snippet: Macrophage infiltration in the heart tissues and plasma levels of proinflammatory cytokines. a Macrophage infiltration into mouse heart tissues at week 12 visualized by F4/80 staining with 20× magnification of the interventricular septum region shown in the lower right corner. Scale bar equals 2 mm and 60 µm. A single representative section for each cohort is shown. b – e Plasma cytokines were determined by electrochemiluminescence assay at week 3: b CCL2; c CXCL10; d CXCL1; and e TNF-α. WT-chow ( n = 10), DKO-chow ( n = 12), WT-PSRD ( n = 9), DKO-PSRD ( n = 22). Data were analyzed using one-way ANOVA and presented using box-and-whisker plot, where the boxes encompass the first to the third quartiles, inside the box the horizontal line shows the median, and the whiskers are the maximum and minimum observation. * p

    Article Snippet: Sections were also stained with rat anti-F4/80 antibody (1:2000, BioRad MCA497R/Lot 1014) for 60 min, washed, followed by incubation with secondary antibody (Rat 1-Step Polymer, Biocare BRR4016L).

    Techniques: Staining, Electrochemiluminescence, Whisker Assay

    Rubulavirus SV5 V gene induces Stat1 ubiquitination but not pY701-Stat1 formation. 2fTGH cells were cotransfected with plasmids expressing GFP and Stat1 along with the SeV C , SeV C Δ10-15 , or SV5 V gene or an empty plasmid. Total cell extracts were prepared at 24 h posttransfection, and equal amounts of extract were Western blotted with either anti-Stat1 (bulk, top panel) or anti-pY701-Stat1 plus antiactin (middle panel). Equal amounts of extract were also precipitated with antiubiquitin (Ub) and then Western blotted with anti-Stat1 (bottom panel). The right-hand lane in the bottom panel has not been immunoprecipitated and shows the electrophoretic position of bulk Stat1. IgG-hc, IgG heavy chain.

    Journal: Journal of Virology

    Article Title: The Amino-Terminal Extensions of the Longer Sendai Virus C Proteins Modulate pY701-Stat1 and Bulk Stat1 Levels Independently of Interferon Signaling

    doi: 10.1128/JVI.77.4.2321-2329.2003

    Figure Lengend Snippet: Rubulavirus SV5 V gene induces Stat1 ubiquitination but not pY701-Stat1 formation. 2fTGH cells were cotransfected with plasmids expressing GFP and Stat1 along with the SeV C , SeV C Δ10-15 , or SV5 V gene or an empty plasmid. Total cell extracts were prepared at 24 h posttransfection, and equal amounts of extract were Western blotted with either anti-Stat1 (bulk, top panel) or anti-pY701-Stat1 plus antiactin (middle panel). Equal amounts of extract were also precipitated with antiubiquitin (Ub) and then Western blotted with anti-Stat1 (bottom panel). The right-hand lane in the bottom panel has not been immunoprecipitated and shows the electrophoretic position of bulk Stat1. IgG-hc, IgG heavy chain.

    Article Snippet: The secondary antibodies used were alkaline phosphatase-conjugated goat antibodies specific for either rabbit or mouse immunoglobulin G (Bio-Rad).

    Techniques: Expressing, Plasmid Preparation, Western Blot, Immunoprecipitation