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Jackson Immuno rabbit anti rat igg
Rabbit Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti rat igg/product/Jackson Immuno
Average 91 stars, based on 4 article reviews
Price from $9.99 to $1999.99
rabbit anti rat igg - by Bioz Stars, 2020-09
91/100 stars

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Article Title: Origin of Androgen-Insensitive Poorly Differentiated Tumors in the Transgenic Adenocarcinoma of Mouse Prostate Model 1Origin of Androgen-Insensitive Poorly Differentiated Tumors in the Transgenic Adenocarcinoma of Mouse Prostate Model 1 2
Article Snippet: .. All slides were incubated with the appropriate biotinylated secondary antibody: goat anti-rabbit IgG (Vector, Burlingame, CA); rabbit anti-rat IgG (Vector); goat anti-mouse IgG (Vector); or rabbit anti-goat IgG (Jackson ImmunoResearch Laboratories, Inc.,West Grove, PA) at a 1:1000 dilution for 20 minutes at 37°C. .. Immunoreactive antigens were detected using the Vectastain Elite ABC Immunoperoxidase Kit and DAB, Nova Red (Vector), TrueBlue (KPL, Gaithersburg, MD), or ABC Alkaline Phosphatase Kit I or III (Vector).Omission of primary antibody, and tissue from animals not injected with BrdUrd (for BrdUrd staining), served as negative controls, and positive control tissues (small intestine for BrdUrd, Ki67, synaptophysin, and ABCG2 staining, and prostate from intact TRAMP mouse for SV40 and AR staining) were included for each experiment.

Plasmid Preparation:

Article Title: Origin of Androgen-Insensitive Poorly Differentiated Tumors in the Transgenic Adenocarcinoma of Mouse Prostate Model 1Origin of Androgen-Insensitive Poorly Differentiated Tumors in the Transgenic Adenocarcinoma of Mouse Prostate Model 1 2
Article Snippet: .. All slides were incubated with the appropriate biotinylated secondary antibody: goat anti-rabbit IgG (Vector, Burlingame, CA); rabbit anti-rat IgG (Vector); goat anti-mouse IgG (Vector); or rabbit anti-goat IgG (Jackson ImmunoResearch Laboratories, Inc.,West Grove, PA) at a 1:1000 dilution for 20 minutes at 37°C. .. Immunoreactive antigens were detected using the Vectastain Elite ABC Immunoperoxidase Kit and DAB, Nova Red (Vector), TrueBlue (KPL, Gaithersburg, MD), or ABC Alkaline Phosphatase Kit I or III (Vector).Omission of primary antibody, and tissue from animals not injected with BrdUrd (for BrdUrd staining), served as negative controls, and positive control tissues (small intestine for BrdUrd, Ki67, synaptophysin, and ABCG2 staining, and prostate from intact TRAMP mouse for SV40 and AR staining) were included for each experiment.

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    Jackson Immuno fitc conjugated anti rabbit igg antibody
    Inhibition of the PI3K/AKT/mTOR pathway does not affect total GLUT1 protein expression but alters membrane-bound GLUT1 levels in EGFR -mutant LAD cells. A , Western blot analysis showing GLUT1 and β-actin as a loading control in HCC827, PC-9, and H1975 cells treated with the indicated inhibitors. Equivalent amounts of proteins from whole cell lysates were subjected to WB analysis to detect total GLUT1 proteins. For flow cytometric analysis, LAD cells were treated with ERLO (1 μ m ), BEZ235 (1 μ m ) or DMSO as a control for 6 h. After fixation, cells were stained with a rabbit anti-GLUT1 antibody and <t>FITC-conjugated</t> anti-rabbit secondary antibody. B—D , representative flow cytometry plots of GLUT1 expression in HCC827 ( B ), PC-9 ( C ), and H1975 ( D ) cells treated with DMSO or BEZ235. E—G , mean fluorescence intensity for GLUT1 for HCC827 ( E ), PC-9 ( F ), and H1975 ( G ) cells. BEZ , BEZ235. Blue bars show background fluorescence with the <t>IgG</t> isotype control, whereas red bars indicate fluorescence staining results with anti-GLUT1 antibody. Error bars indicate mean ± S.D. ( n = 3). *, p
    Fitc Conjugated Anti Rabbit Igg Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated anti rabbit igg antibody/product/Jackson Immuno
    Average 99 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated anti rabbit igg antibody - by Bioz Stars, 2020-09
    99/100 stars
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    Inhibition of the PI3K/AKT/mTOR pathway does not affect total GLUT1 protein expression but alters membrane-bound GLUT1 levels in EGFR -mutant LAD cells. A , Western blot analysis showing GLUT1 and β-actin as a loading control in HCC827, PC-9, and H1975 cells treated with the indicated inhibitors. Equivalent amounts of proteins from whole cell lysates were subjected to WB analysis to detect total GLUT1 proteins. For flow cytometric analysis, LAD cells were treated with ERLO (1 μ m ), BEZ235 (1 μ m ) or DMSO as a control for 6 h. After fixation, cells were stained with a rabbit anti-GLUT1 antibody and FITC-conjugated anti-rabbit secondary antibody. B—D , representative flow cytometry plots of GLUT1 expression in HCC827 ( B ), PC-9 ( C ), and H1975 ( D ) cells treated with DMSO or BEZ235. E—G , mean fluorescence intensity for GLUT1 for HCC827 ( E ), PC-9 ( F ), and H1975 ( G ) cells. BEZ , BEZ235. Blue bars show background fluorescence with the IgG isotype control, whereas red bars indicate fluorescence staining results with anti-GLUT1 antibody. Error bars indicate mean ± S.D. ( n = 3). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Signaling through the Phosphatidylinositol 3-Kinase (PI3K)/Mammalian Target of Rapamycin (mTOR) Axis Is Responsible for Aerobic Glycolysis mediated by Glucose Transporter in Epidermal Growth Factor Receptor (EGFR)-mutated Lung Adenocarcinoma *

    doi: 10.1074/jbc.M115.660498

    Figure Lengend Snippet: Inhibition of the PI3K/AKT/mTOR pathway does not affect total GLUT1 protein expression but alters membrane-bound GLUT1 levels in EGFR -mutant LAD cells. A , Western blot analysis showing GLUT1 and β-actin as a loading control in HCC827, PC-9, and H1975 cells treated with the indicated inhibitors. Equivalent amounts of proteins from whole cell lysates were subjected to WB analysis to detect total GLUT1 proteins. For flow cytometric analysis, LAD cells were treated with ERLO (1 μ m ), BEZ235 (1 μ m ) or DMSO as a control for 6 h. After fixation, cells were stained with a rabbit anti-GLUT1 antibody and FITC-conjugated anti-rabbit secondary antibody. B—D , representative flow cytometry plots of GLUT1 expression in HCC827 ( B ), PC-9 ( C ), and H1975 ( D ) cells treated with DMSO or BEZ235. E—G , mean fluorescence intensity for GLUT1 for HCC827 ( E ), PC-9 ( F ), and H1975 ( G ) cells. BEZ , BEZ235. Blue bars show background fluorescence with the IgG isotype control, whereas red bars indicate fluorescence staining results with anti-GLUT1 antibody. Error bars indicate mean ± S.D. ( n = 3). *, p

    Article Snippet: Flow Cytometric Analysis To quantitatively detect the expression of membrane-bound GLUT1, cells were fixed with 80% ethanol, incubated with anti-GLUT1 antibody (Abcam), and then stained with the appropriate FITC-conjugated anti-rabbit IgG antibody (Jackson ImmunoResearch Laboratories).

    Techniques: Inhibition, Expressing, Mutagenesis, Western Blot, Flow Cytometry, Staining, Cytometry, Fluorescence

    Collapse of the nucleocytoplasmic Ran gradient causes an equilibration of RanBP1 across the nuclear envelope. The nuclei of BHK-21 cells were microinjected with FITC-dextran (0.2 mg/ml) as a site-of-injection marker (left) along with either wild-type (WT) Ran (1.0 mg/ml; a panels) or RanGAP (0.4 mg/ml; b panels) and incubated for 10 min at 37°C. The intracellular distribution of endogenous RanBP1 was assessed by indirect immunofluorescence (IF) with anti-RanBP1 and Texas red-conjugated to anti-goat IgG (right) as described in Materials and Methods. Arrows are drawn to help distinguish injected cells from uninjected cells.

    Journal: Molecular and Cellular Biology

    Article Title: Facilitated Nucleocytoplasmic Shuttling of the Ran Binding Protein RanBP1

    doi:

    Figure Lengend Snippet: Collapse of the nucleocytoplasmic Ran gradient causes an equilibration of RanBP1 across the nuclear envelope. The nuclei of BHK-21 cells were microinjected with FITC-dextran (0.2 mg/ml) as a site-of-injection marker (left) along with either wild-type (WT) Ran (1.0 mg/ml; a panels) or RanGAP (0.4 mg/ml; b panels) and incubated for 10 min at 37°C. The intracellular distribution of endogenous RanBP1 was assessed by indirect immunofluorescence (IF) with anti-RanBP1 and Texas red-conjugated to anti-goat IgG (right) as described in Materials and Methods. Arrows are drawn to help distinguish injected cells from uninjected cells.

    Article Snippet: All other cells were blocked in 3% BSA–PBS; incubated with a 1:500 dilution of anti-GST monoclonal antibody (MAb; Santa Cruz catalog SC138), a 1:200 dilution of anti-Ran or 1:200 dilution of anti-RanBP1 polyclonal antibody (Santa Cruz catalog SC1159) as primary antibody, and Texas red-conjugated anti-mouse immunoglobulin G (IgG), Texas red-conjugated anti-goat IgG, or FITC-conjugated anti-rabbit IgG (all from Jackson ImmunoResearch Laboratories, diluted 1:500), with DAPI (10 ng/ml); and mounted and viewed as described above.

    Techniques: Injection, Marker, Incubation, Immunofluorescence

    Effect of transduced of Tat-Atox1 protein on animal brain. Transduction of Tat-Atox1 protein into brain (A). Animals were treated with single i.p. injection of Tat-Atox1 protein and killed after 6 hrs. Transduction of Tat-Atox1 protein into the CA1 region was determined by immunohistochemistry with a rabbit anti-polyhistidine antibody and FITC-conjugated anti-rabbit IgG. Immunohistochemistry for NeuN in the hippocampal CA1 region (B). Control, ischaemia, Tat peptide, Atox1 protein and Tat-Atox1 protein-treated groups 4 days after ischaemia-reperfusion. SP, stratum pyramidale; SO, stratum oriens; SR, stratum radiatum; bar = 50 μm. The relative number of NeuN-immunoreactive neurons versus control group per section in all the groups ( n = 5 per group; * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Tat-antioxidant 1 protects against stress-induced hippocampal HT-22 cells death and attenuate ischaemic insult in animal model

    doi: 10.1111/jcmm.12513

    Figure Lengend Snippet: Effect of transduced of Tat-Atox1 protein on animal brain. Transduction of Tat-Atox1 protein into brain (A). Animals were treated with single i.p. injection of Tat-Atox1 protein and killed after 6 hrs. Transduction of Tat-Atox1 protein into the CA1 region was determined by immunohistochemistry with a rabbit anti-polyhistidine antibody and FITC-conjugated anti-rabbit IgG. Immunohistochemistry for NeuN in the hippocampal CA1 region (B). Control, ischaemia, Tat peptide, Atox1 protein and Tat-Atox1 protein-treated groups 4 days after ischaemia-reperfusion. SP, stratum pyramidale; SO, stratum oriens; SR, stratum radiatum; bar = 50 μm. The relative number of NeuN-immunoreactive neurons versus control group per section in all the groups ( n = 5 per group; * P

    Article Snippet: Protective effects of Tat-Atox1 protein against ischaemic damage To assess the protective effects of Tat-Atox1 on ischaemic injury, the transduction of Tat-Atox1 protein into the hippocampal CA1 region of gerbils, crossing the blood-brain barrier (BBB), was measured by immunohistochemial analysis using rabbit anti-polyhistidine antibody and visualized with an FITC-conjugated anti-rabbit IgG (1:200; Jackson ImmunoResearch, West Grove, PA, USA).

    Techniques: Transduction, Injection, Immunohistochemistry

    Glucose transport efficiency is the most rapid and critically regulated function of glucose metabolism linked to EGFR signaling. A , representative flow cytometry plot of GLUT3 expression in HCC827 cells treated with erlotinib (1 μ m ) or DMSO as a control for 6 h. After fixation, cells were stained with a rabbit anti-GLUT3 antibody and FITC-conjugated anti-rabbit secondary antibody. B , flow cytometric analysis of GLUT3 expression in HCC827 cells. Blue bars show background fluorescence with IgG isotype control while red bars indicate fluorescence staining results with anti-GLUT3 Ab. Error bars indicate S.D. ( n = 4). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Epidermal Growth Factor Receptor (EGFR) Signaling Regulates Global Metabolic Pathways in EGFR-mutated Lung Adenocarcinoma *

    doi: 10.1074/jbc.M114.575464

    Figure Lengend Snippet: Glucose transport efficiency is the most rapid and critically regulated function of glucose metabolism linked to EGFR signaling. A , representative flow cytometry plot of GLUT3 expression in HCC827 cells treated with erlotinib (1 μ m ) or DMSO as a control for 6 h. After fixation, cells were stained with a rabbit anti-GLUT3 antibody and FITC-conjugated anti-rabbit secondary antibody. B , flow cytometric analysis of GLUT3 expression in HCC827 cells. Blue bars show background fluorescence with IgG isotype control while red bars indicate fluorescence staining results with anti-GLUT3 Ab. Error bars indicate S.D. ( n = 4). *, p

    Article Snippet: Expression of Glucose Transporter and Glucose Transport Assay To detect expression of membrane-bound GLUTs, cells were fixed with 80% ethanol and incubated with anti-GLUT3 antibody (Abcam) and stained with the appropriate FITC-conjugated anti-rabbit IgG antibody (Jackson Immuno Research).

    Techniques: Flow Cytometry, Cytometry, Expressing, Staining, Fluorescence