rabbit anti potassium channel kv11 1 extracellular antibody  (Millipore)

 
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    Millipore rabbit anti potassium channel kv11 1 extracellular antibody
    Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one <t>Kv11.1</t> channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.
    Rabbit Anti Potassium Channel Kv11 1 Extracellular Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti potassium channel kv11 1 extracellular antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti potassium channel kv11 1 extracellular antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "H1153Y- KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating"

    Article Title: H1153Y- KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22179235

    Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one Kv11.1 channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.
    Figure Legend Snippet: Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one Kv11.1 channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.

    Techniques Used: Variant Assay, Sequencing

    Representative microscopy images of HEK293 cells expressing WT-Kv11.1 and H1153Y-Kv11.1. GFP fluorescence is shown in green. Anti-Kv11.1 extracellular immunostaining (Alexa 647 fluorescence) is shown in red. Overlapping immunostaining of red and green signals of similar intensities is shown as yellow, and the cell nuclei are labeled blue. The scale bar represents 100 µm at 20× magnification and 50 µM at 63× magnification.
    Figure Legend Snippet: Representative microscopy images of HEK293 cells expressing WT-Kv11.1 and H1153Y-Kv11.1. GFP fluorescence is shown in green. Anti-Kv11.1 extracellular immunostaining (Alexa 647 fluorescence) is shown in red. Overlapping immunostaining of red and green signals of similar intensities is shown as yellow, and the cell nuclei are labeled blue. The scale bar represents 100 µm at 20× magnification and 50 µM at 63× magnification.

    Techniques Used: Microscopy, Expressing, Fluorescence, Immunostaining, Labeling

    Flow cytometry qualitative ( A ) and quantitative ( B , C ) assessment of WT-Kv11.1 and H1153-Kv11.1 channel expression. ( A ) Representative histograms for unstained cells (corresponding to non-transfected cells) and cells transiently transfected with WT-Kv11.1 and H153Y-Kv11.1 stained with antiKv11.1 conjugated antibody. ( B ) Quantification of surface Kv11.1 channel (Alexia 647 fluorescence) for WT and H1153 variants in non-permeabilized cells. ( C ) Quantification of total Kv11.1 channel (GFP fused to the N-terminus of Kv11.1) for WT and H1153-Kv11.1 in non-permeabilized cells. The Median Kv11.1 Intensity of Fluorescence (MIF) represents the median of fluorescence intensity of 10,000 cells counted for each experiment ( p > 0.05). Each value represents the median+ S.E.M. of four independent experiments.
    Figure Legend Snippet: Flow cytometry qualitative ( A ) and quantitative ( B , C ) assessment of WT-Kv11.1 and H1153-Kv11.1 channel expression. ( A ) Representative histograms for unstained cells (corresponding to non-transfected cells) and cells transiently transfected with WT-Kv11.1 and H153Y-Kv11.1 stained with antiKv11.1 conjugated antibody. ( B ) Quantification of surface Kv11.1 channel (Alexia 647 fluorescence) for WT and H1153 variants in non-permeabilized cells. ( C ) Quantification of total Kv11.1 channel (GFP fused to the N-terminus of Kv11.1) for WT and H1153-Kv11.1 in non-permeabilized cells. The Median Kv11.1 Intensity of Fluorescence (MIF) represents the median of fluorescence intensity of 10,000 cells counted for each experiment ( p > 0.05). Each value represents the median+ S.E.M. of four independent experiments.

    Techniques Used: Flow Cytometry, Expressing, Transfection, Staining, Fluorescence

    Trafficking phenotype of WT-Kv11.1 and H1153-Kv11.1 channel protein. ( A ) Representative Western blot analyses of HEK293 cells transiently transfected with pcDNA3.1-N-eGFP (control cells), WT-Kv11.1, or H1153-Kv11.1, with or without pK treatment (200 μg/mL, 30 min); Kv11.1 channel protein was detected with an anti-Kv11.1 antibody targeting intracellular domain. Kv11.1 WT and H1153 showed two bands corresponding to a mature complex-glycosylated 182 kDA Kv11.1 band (155 kD + 27 kDA of GFP) completely digested by pK and one band at slightly lower molecular weight (162 kD) resistant to pK cleavage corresponding to immature core-glycosylated Kv11.1 protein band. ( B – D ) Semi-quantification of mature ( B ) and immature proteins ( C ) in WT and H1153Y cells with and without pK. Total protein measured with stain-free method was used to normalize, n = 5 independent experiments (** ( p < 0.01) = for difference between WT and pK-treated WT, and for difference between mutant and pK-treated mutant; ns = no significant difference ( p > 0.05) between mutant and WT. ( D ) The relative amount of mature Kv11.1 protein (mature/total Kv11.1) based on immunoblot analyses for each set of experiments is plotted ( p > 0.05). The error bars represent SEM.
    Figure Legend Snippet: Trafficking phenotype of WT-Kv11.1 and H1153-Kv11.1 channel protein. ( A ) Representative Western blot analyses of HEK293 cells transiently transfected with pcDNA3.1-N-eGFP (control cells), WT-Kv11.1, or H1153-Kv11.1, with or without pK treatment (200 μg/mL, 30 min); Kv11.1 channel protein was detected with an anti-Kv11.1 antibody targeting intracellular domain. Kv11.1 WT and H1153 showed two bands corresponding to a mature complex-glycosylated 182 kDA Kv11.1 band (155 kD + 27 kDA of GFP) completely digested by pK and one band at slightly lower molecular weight (162 kD) resistant to pK cleavage corresponding to immature core-glycosylated Kv11.1 protein band. ( B – D ) Semi-quantification of mature ( B ) and immature proteins ( C ) in WT and H1153Y cells with and without pK. Total protein measured with stain-free method was used to normalize, n = 5 independent experiments (** ( p < 0.01) = for difference between WT and pK-treated WT, and for difference between mutant and pK-treated mutant; ns = no significant difference ( p > 0.05) between mutant and WT. ( D ) The relative amount of mature Kv11.1 protein (mature/total Kv11.1) based on immunoblot analyses for each set of experiments is plotted ( p > 0.05). The error bars represent SEM.

    Techniques Used: Western Blot, Transfection, Molecular Weight, Staining, Mutagenesis

    rabbit anti potassium channel kv11 1 extracellular antibody  (Millipore)

     
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    Millipore rabbit anti potassium channel kv11 1 extracellular antibody
    Rabbit Anti Potassium Channel Kv11 1 Extracellular Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti potassium channel kv11 1 extracellular antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti potassium channel kv11 1 extracellular antibody - by Bioz Stars, 2024-07
    86/100 stars

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    rabbit anti potassium channel kv11 1 extracellular antibody  (Millipore)

     
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    Structured Review

    Millipore rabbit anti potassium channel kv11 1 extracellular antibody
    Rabbit Anti Potassium Channel Kv11 1 Extracellular Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti potassium channel kv11 1 extracellular antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti potassium channel kv11 1 extracellular antibody - by Bioz Stars, 2024-07
    86/100 stars

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    rabbit polyclonal anti potassium channel kv11 1 extracellular antibody  (Millipore)

     
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    Structured Review

    Millipore rabbit polyclonal anti potassium channel kv11 1 extracellular antibody
    Rabbit Polyclonal Anti Potassium Channel Kv11 1 Extracellular Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti potassium channel kv11 1 extracellular antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti potassium channel kv11 1 extracellular antibody - by Bioz Stars, 2024-07
    86/100 stars

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    Millipore rabbit anti potassium channel kv11 1 extracellular antibody
    Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one <t>Kv11.1</t> channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.
    Rabbit Anti Potassium Channel Kv11 1 Extracellular Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti potassium channel kv11 1 extracellular antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti potassium channel kv11 1 extracellular antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Millipore rabbit polyclonal anti potassium channel kv11 1 extracellular antibody
    Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one <t>Kv11.1</t> channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.
    Rabbit Polyclonal Anti Potassium Channel Kv11 1 Extracellular Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti potassium channel kv11 1 extracellular antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti potassium channel kv11 1 extracellular antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one Kv11.1 channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.

    Journal: International Journal of Molecular Sciences

    Article Title: H1153Y- KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating

    doi: 10.3390/ijms22179235

    Figure Lengend Snippet: Molecular position of the KCNH2 H1153Y variant (NM_0002383; rs 199473035) detected using the Ion torrent TM PGM platform and confirmed with Sanger sequencing. ( A ) The reads of NGS sequencing and the DNA sequence chromatogram illustrate the heterozygous c:3457C > T nucleotide substitution that results in the substitution of a Histidine-H (positively charged amino acid) for a Tyrosine-Y (polar uncharged amino acid) at amino acid residue 1153. ( B ) Cartoon of one Kv11.1 channel subunit. The circle denotes the approximate location of the p.H1153Y variant in the C-term region.

    Article Snippet: They were then incubated with rabbit anti-potassium channel Kv11.1 extracellular antibody (1:16; Sigma P0749; Sigma-Aldrich, Saint Louis, MO, USA) for 30 min at 4 °C, rinsed twice with PBS, and incubated with goat anti-rabbit antibody conjugated to Alexa Fluor 647 (1:400; Abcam 150087, Cambridge, UK) for 30 min at 4 °C.

    Techniques: Variant Assay, Sequencing

    Representative microscopy images of HEK293 cells expressing WT-Kv11.1 and H1153Y-Kv11.1. GFP fluorescence is shown in green. Anti-Kv11.1 extracellular immunostaining (Alexa 647 fluorescence) is shown in red. Overlapping immunostaining of red and green signals of similar intensities is shown as yellow, and the cell nuclei are labeled blue. The scale bar represents 100 µm at 20× magnification and 50 µM at 63× magnification.

    Journal: International Journal of Molecular Sciences

    Article Title: H1153Y- KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating

    doi: 10.3390/ijms22179235

    Figure Lengend Snippet: Representative microscopy images of HEK293 cells expressing WT-Kv11.1 and H1153Y-Kv11.1. GFP fluorescence is shown in green. Anti-Kv11.1 extracellular immunostaining (Alexa 647 fluorescence) is shown in red. Overlapping immunostaining of red and green signals of similar intensities is shown as yellow, and the cell nuclei are labeled blue. The scale bar represents 100 µm at 20× magnification and 50 µM at 63× magnification.

    Article Snippet: They were then incubated with rabbit anti-potassium channel Kv11.1 extracellular antibody (1:16; Sigma P0749; Sigma-Aldrich, Saint Louis, MO, USA) for 30 min at 4 °C, rinsed twice with PBS, and incubated with goat anti-rabbit antibody conjugated to Alexa Fluor 647 (1:400; Abcam 150087, Cambridge, UK) for 30 min at 4 °C.

    Techniques: Microscopy, Expressing, Fluorescence, Immunostaining, Labeling

    Flow cytometry qualitative ( A ) and quantitative ( B , C ) assessment of WT-Kv11.1 and H1153-Kv11.1 channel expression. ( A ) Representative histograms for unstained cells (corresponding to non-transfected cells) and cells transiently transfected with WT-Kv11.1 and H153Y-Kv11.1 stained with antiKv11.1 conjugated antibody. ( B ) Quantification of surface Kv11.1 channel (Alexia 647 fluorescence) for WT and H1153 variants in non-permeabilized cells. ( C ) Quantification of total Kv11.1 channel (GFP fused to the N-terminus of Kv11.1) for WT and H1153-Kv11.1 in non-permeabilized cells. The Median Kv11.1 Intensity of Fluorescence (MIF) represents the median of fluorescence intensity of 10,000 cells counted for each experiment ( p > 0.05). Each value represents the median+ S.E.M. of four independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: H1153Y- KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating

    doi: 10.3390/ijms22179235

    Figure Lengend Snippet: Flow cytometry qualitative ( A ) and quantitative ( B , C ) assessment of WT-Kv11.1 and H1153-Kv11.1 channel expression. ( A ) Representative histograms for unstained cells (corresponding to non-transfected cells) and cells transiently transfected with WT-Kv11.1 and H153Y-Kv11.1 stained with antiKv11.1 conjugated antibody. ( B ) Quantification of surface Kv11.1 channel (Alexia 647 fluorescence) for WT and H1153 variants in non-permeabilized cells. ( C ) Quantification of total Kv11.1 channel (GFP fused to the N-terminus of Kv11.1) for WT and H1153-Kv11.1 in non-permeabilized cells. The Median Kv11.1 Intensity of Fluorescence (MIF) represents the median of fluorescence intensity of 10,000 cells counted for each experiment ( p > 0.05). Each value represents the median+ S.E.M. of four independent experiments.

    Article Snippet: They were then incubated with rabbit anti-potassium channel Kv11.1 extracellular antibody (1:16; Sigma P0749; Sigma-Aldrich, Saint Louis, MO, USA) for 30 min at 4 °C, rinsed twice with PBS, and incubated with goat anti-rabbit antibody conjugated to Alexa Fluor 647 (1:400; Abcam 150087, Cambridge, UK) for 30 min at 4 °C.

    Techniques: Flow Cytometry, Expressing, Transfection, Staining, Fluorescence

    Trafficking phenotype of WT-Kv11.1 and H1153-Kv11.1 channel protein. ( A ) Representative Western blot analyses of HEK293 cells transiently transfected with pcDNA3.1-N-eGFP (control cells), WT-Kv11.1, or H1153-Kv11.1, with or without pK treatment (200 μg/mL, 30 min); Kv11.1 channel protein was detected with an anti-Kv11.1 antibody targeting intracellular domain. Kv11.1 WT and H1153 showed two bands corresponding to a mature complex-glycosylated 182 kDA Kv11.1 band (155 kD + 27 kDA of GFP) completely digested by pK and one band at slightly lower molecular weight (162 kD) resistant to pK cleavage corresponding to immature core-glycosylated Kv11.1 protein band. ( B – D ) Semi-quantification of mature ( B ) and immature proteins ( C ) in WT and H1153Y cells with and without pK. Total protein measured with stain-free method was used to normalize, n = 5 independent experiments (** ( p < 0.01) = for difference between WT and pK-treated WT, and for difference between mutant and pK-treated mutant; ns = no significant difference ( p > 0.05) between mutant and WT. ( D ) The relative amount of mature Kv11.1 protein (mature/total Kv11.1) based on immunoblot analyses for each set of experiments is plotted ( p > 0.05). The error bars represent SEM.

    Journal: International Journal of Molecular Sciences

    Article Title: H1153Y- KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating

    doi: 10.3390/ijms22179235

    Figure Lengend Snippet: Trafficking phenotype of WT-Kv11.1 and H1153-Kv11.1 channel protein. ( A ) Representative Western blot analyses of HEK293 cells transiently transfected with pcDNA3.1-N-eGFP (control cells), WT-Kv11.1, or H1153-Kv11.1, with or without pK treatment (200 μg/mL, 30 min); Kv11.1 channel protein was detected with an anti-Kv11.1 antibody targeting intracellular domain. Kv11.1 WT and H1153 showed two bands corresponding to a mature complex-glycosylated 182 kDA Kv11.1 band (155 kD + 27 kDA of GFP) completely digested by pK and one band at slightly lower molecular weight (162 kD) resistant to pK cleavage corresponding to immature core-glycosylated Kv11.1 protein band. ( B – D ) Semi-quantification of mature ( B ) and immature proteins ( C ) in WT and H1153Y cells with and without pK. Total protein measured with stain-free method was used to normalize, n = 5 independent experiments (** ( p < 0.01) = for difference between WT and pK-treated WT, and for difference between mutant and pK-treated mutant; ns = no significant difference ( p > 0.05) between mutant and WT. ( D ) The relative amount of mature Kv11.1 protein (mature/total Kv11.1) based on immunoblot analyses for each set of experiments is plotted ( p > 0.05). The error bars represent SEM.

    Article Snippet: They were then incubated with rabbit anti-potassium channel Kv11.1 extracellular antibody (1:16; Sigma P0749; Sigma-Aldrich, Saint Louis, MO, USA) for 30 min at 4 °C, rinsed twice with PBS, and incubated with goat anti-rabbit antibody conjugated to Alexa Fluor 647 (1:400; Abcam 150087, Cambridge, UK) for 30 min at 4 °C.

    Techniques: Western Blot, Transfection, Molecular Weight, Staining, Mutagenesis