rabbit polyclonal anti phospho synapsin 1 ser9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti phospho synapsin 1 ser9
    Sema5A enhances the density of <t>synapsin</t> <t>1</t> clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also <xref ref-type=Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. " width="250" height="auto" />
    Rabbit Polyclonal Anti Phospho Synapsin 1 Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti phospho synapsin 1 ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti phospho synapsin 1 ser9 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation"

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    Journal: iScience

    doi: 10.1016/j.isci.2024.109638

    Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also <xref ref-type=Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. " title="Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test.

    Techniques Used: Expressing, Transfection, Western Blot, Immunofluorescence

    Sema5A increases enteric neurons synapsin 1 phosphorylation at site Ser-603 Western blot analysis of synapsin 1 phosphorylation at site Ser603, Ser9 and Ser62/67 from enteric neuron cultures exposed for 3 days to control Fc, Sema5A-Fc, or Sema5A-S956G-Fc. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.
    Figure Legend Snippet: Sema5A increases enteric neurons synapsin 1 phosphorylation at site Ser-603 Western blot analysis of synapsin 1 phosphorylation at site Ser603, Ser9 and Ser62/67 from enteric neuron cultures exposed for 3 days to control Fc, Sema5A-Fc, or Sema5A-S956G-Fc. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Techniques Used: Western Blot

    KCl-induced synaptic vesicle exocytosis is reduced by Sema5A in association with a decrease in synapsin 1 phosphorylation at site Ser-603 and Ser-9 (A) From left to right, merged picture of FM1-43 fluorescence and brightfield microscopy at t0s. Representative images of enteric neurons labeled with FM1-43 at t0s and t4 min after 90 mM KCl stimulation. (B) FM1-43 fluorescence decreases over 4 min exposure to 90 mM KCl. (C) Remaining fluorescence was measured as destaining fraction, after 4 min exposure to KCl. Destaining fraction of FM1-43 fluorescence is expressed as 1-(Fluo t4 min/Fluo at t0). Data are mean ± SEM. ( n = 60 clusters per culture well, from 12 culture wells from 4 independent experiments); ∗∗∗ p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test. (D) Neurons were depolarized by 1 min exposure to 90 mM KCl and collected for western blot analysis and assessment of synapsin 1 phosphorylation at site Ser-603, Ser-9 and Ser-62/67. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.
    Figure Legend Snippet: KCl-induced synaptic vesicle exocytosis is reduced by Sema5A in association with a decrease in synapsin 1 phosphorylation at site Ser-603 and Ser-9 (A) From left to right, merged picture of FM1-43 fluorescence and brightfield microscopy at t0s. Representative images of enteric neurons labeled with FM1-43 at t0s and t4 min after 90 mM KCl stimulation. (B) FM1-43 fluorescence decreases over 4 min exposure to 90 mM KCl. (C) Remaining fluorescence was measured as destaining fraction, after 4 min exposure to KCl. Destaining fraction of FM1-43 fluorescence is expressed as 1-(Fluo t4 min/Fluo at t0). Data are mean ± SEM. ( n = 60 clusters per culture well, from 12 culture wells from 4 independent experiments); ∗∗∗ p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test. (D) Neurons were depolarized by 1 min exposure to 90 mM KCl and collected for western blot analysis and assessment of synapsin 1 phosphorylation at site Ser-603, Ser-9 and Ser-62/67. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Techniques Used: Fluorescence, Microscopy, Labeling, Western Blot

    Enteric neurons exposed to Sema5A for 5 min show increased CaMKII phosphorylation and synapsin 1 phosphorylation at site Ser603 Enteric neurons were treated for 5 min or 30 min with Fc fragment or Sema5A-Fc (4.5 μM). Western blot for phosphorylated CaMKII at Tyr286 (A), phosphorylated ERK at Thr202/Tyr204 (B), and synapsin 1 at Ser-603 (C). ∗ p < 0.05 Mann-Whitney test for each time group. Data are mean ± SEM, n = 12 to 16 cultures from 3 to 4 independent experiments.
    Figure Legend Snippet: Enteric neurons exposed to Sema5A for 5 min show increased CaMKII phosphorylation and synapsin 1 phosphorylation at site Ser603 Enteric neurons were treated for 5 min or 30 min with Fc fragment or Sema5A-Fc (4.5 μM). Western blot for phosphorylated CaMKII at Tyr286 (A), phosphorylated ERK at Thr202/Tyr204 (B), and synapsin 1 at Ser-603 (C). ∗ p < 0.05 Mann-Whitney test for each time group. Data are mean ± SEM, n = 12 to 16 cultures from 3 to 4 independent experiments.

    Techniques Used: Western Blot, MANN-WHITNEY


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Mutagenesis, Bicinchoninic Acid Protein Assay, Software, Membrane

    rabbit polyclonal anti phospho synapsin 1 ser9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti phospho synapsin 1 ser9
    Sema5A enhances the density of <t>synapsin</t> <t>1</t> clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also <xref ref-type=Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. " width="250" height="auto" />
    Rabbit Polyclonal Anti Phospho Synapsin 1 Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti phospho synapsin 1 ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti phospho synapsin 1 ser9 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation"

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    Journal: iScience

    doi: 10.1016/j.isci.2024.109638

    Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also <xref ref-type=Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. " title="Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test.

    Techniques Used: Expressing, Transfection, Western Blot, Immunofluorescence

    Sema5A increases enteric neurons synapsin 1 phosphorylation at site Ser-603 Western blot analysis of synapsin 1 phosphorylation at site Ser603, Ser9 and Ser62/67 from enteric neuron cultures exposed for 3 days to control Fc, Sema5A-Fc, or Sema5A-S956G-Fc. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.
    Figure Legend Snippet: Sema5A increases enteric neurons synapsin 1 phosphorylation at site Ser-603 Western blot analysis of synapsin 1 phosphorylation at site Ser603, Ser9 and Ser62/67 from enteric neuron cultures exposed for 3 days to control Fc, Sema5A-Fc, or Sema5A-S956G-Fc. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Techniques Used: Western Blot

    KCl-induced synaptic vesicle exocytosis is reduced by Sema5A in association with a decrease in synapsin 1 phosphorylation at site Ser-603 and Ser-9 (A) From left to right, merged picture of FM1-43 fluorescence and brightfield microscopy at t0s. Representative images of enteric neurons labeled with FM1-43 at t0s and t4 min after 90 mM KCl stimulation. (B) FM1-43 fluorescence decreases over 4 min exposure to 90 mM KCl. (C) Remaining fluorescence was measured as destaining fraction, after 4 min exposure to KCl. Destaining fraction of FM1-43 fluorescence is expressed as 1-(Fluo t4 min/Fluo at t0). Data are mean ± SEM. ( n = 60 clusters per culture well, from 12 culture wells from 4 independent experiments); ∗∗∗ p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test. (D) Neurons were depolarized by 1 min exposure to 90 mM KCl and collected for western blot analysis and assessment of synapsin 1 phosphorylation at site Ser-603, Ser-9 and Ser-62/67. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.
    Figure Legend Snippet: KCl-induced synaptic vesicle exocytosis is reduced by Sema5A in association with a decrease in synapsin 1 phosphorylation at site Ser-603 and Ser-9 (A) From left to right, merged picture of FM1-43 fluorescence and brightfield microscopy at t0s. Representative images of enteric neurons labeled with FM1-43 at t0s and t4 min after 90 mM KCl stimulation. (B) FM1-43 fluorescence decreases over 4 min exposure to 90 mM KCl. (C) Remaining fluorescence was measured as destaining fraction, after 4 min exposure to KCl. Destaining fraction of FM1-43 fluorescence is expressed as 1-(Fluo t4 min/Fluo at t0). Data are mean ± SEM. ( n = 60 clusters per culture well, from 12 culture wells from 4 independent experiments); ∗∗∗ p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test. (D) Neurons were depolarized by 1 min exposure to 90 mM KCl and collected for western blot analysis and assessment of synapsin 1 phosphorylation at site Ser-603, Ser-9 and Ser-62/67. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Techniques Used: Fluorescence, Microscopy, Labeling, Western Blot

    Enteric neurons exposed to Sema5A for 5 min show increased CaMKII phosphorylation and synapsin 1 phosphorylation at site Ser603 Enteric neurons were treated for 5 min or 30 min with Fc fragment or Sema5A-Fc (4.5 μM). Western blot for phosphorylated CaMKII at Tyr286 (A), phosphorylated ERK at Thr202/Tyr204 (B), and synapsin 1 at Ser-603 (C). ∗ p < 0.05 Mann-Whitney test for each time group. Data are mean ± SEM, n = 12 to 16 cultures from 3 to 4 independent experiments.
    Figure Legend Snippet: Enteric neurons exposed to Sema5A for 5 min show increased CaMKII phosphorylation and synapsin 1 phosphorylation at site Ser603 Enteric neurons were treated for 5 min or 30 min with Fc fragment or Sema5A-Fc (4.5 μM). Western blot for phosphorylated CaMKII at Tyr286 (A), phosphorylated ERK at Thr202/Tyr204 (B), and synapsin 1 at Ser-603 (C). ∗ p < 0.05 Mann-Whitney test for each time group. Data are mean ± SEM, n = 12 to 16 cultures from 3 to 4 independent experiments.

    Techniques Used: Western Blot, MANN-WHITNEY


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Mutagenesis, Bicinchoninic Acid Protein Assay, Software, Membrane

    rabbit monoclonal anti phospho gsk 3ß ser9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho gsk 3ß ser9
    Reagent resources and list of primers
    Rabbit Monoclonal Anti Phospho Gsk 3ß Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho gsk 3ß ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho gsk 3ß ser9 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Disruption of lysosomal nutrient sensing scaffold contributes to pathogenesis of a fatal neurodegenerative lysosomal storage disease"

    Article Title: Disruption of lysosomal nutrient sensing scaffold contributes to pathogenesis of a fatal neurodegenerative lysosomal storage disease

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2024.105641

    Reagent resources and list of primers
    Figure Legend Snippet: Reagent resources and list of primers

    Techniques Used: Electron Microscopy, Recombinant, Protease Inhibitor, Staining, Isolation, Membrane, Enzyme-linked Immunosorbent Assay, Activation Assay, Mutagenesis

    rabbit anti phospho gsk3β ser9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti phospho gsk3β ser9
    KEY RESOURCES TABLE
    Rabbit Anti Phospho Gsk3β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho gsk3β ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti phospho gsk3β ser9 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration"

    Article Title: Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.112647

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Staining, SYBR Green Assay, Lysis, Protease Inhibitor, Generated, Transgenic Assay, Software

    rabbit anti phospho gsk 3β ser9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti phospho gsk 3β ser9
    Rabbit Anti Phospho Gsk 3β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho gsk 3β ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti phospho gsk 3β ser9 - by Bioz Stars, 2024-06
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    resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology
    Resource Source Identifier Antibodies Phospho Gsk 3b Ser9 Rabbit Pab Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology - by Bioz Stars, 2024-06
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    rabbit monoclonal anti phospho gsk 3β ser9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho gsk 3β ser9
    Antibody information.
    Rabbit Monoclonal Anti Phospho Gsk 3β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho gsk 3β ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho gsk 3β ser9 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "The γ-Adducin 1–357 fragment promotes tau pathology"

    Article Title: The γ-Adducin 1–357 fragment promotes tau pathology

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2023.1241750

    Antibody information.
    Figure Legend Snippet: Antibody information.

    Techniques Used: Western Blot, Immunofluorescence

    GSK-3β mediates tau phosphorylation induced by γ-adducin 1–357. (A,B) Western blot of the levels of CDK5 (Cyclin-dependent kinase 5), Akt (Protein kinase B), and GSK-3β (Glycogen synthase kinase-3β) in primary neurons. (C,D) Western blot analysis of the levels of GSK3β, p-tau Ser396 (phosphorylated tau at Ser396), and total tau in primary neurons with or without TDZD-8 (specific kinase inhibitor TDZD-8). Data were presented as mean ± SD, n = 6 independent experiments. n.s. not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by one-way ANOVA.
    Figure Legend Snippet: GSK-3β mediates tau phosphorylation induced by γ-adducin 1–357. (A,B) Western blot of the levels of CDK5 (Cyclin-dependent kinase 5), Akt (Protein kinase B), and GSK-3β (Glycogen synthase kinase-3β) in primary neurons. (C,D) Western blot analysis of the levels of GSK3β, p-tau Ser396 (phosphorylated tau at Ser396), and total tau in primary neurons with or without TDZD-8 (specific kinase inhibitor TDZD-8). Data were presented as mean ± SD, n = 6 independent experiments. n.s. not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by one-way ANOVA.

    Techniques Used: Western Blot

    rabbit anti phospho gsk3β ser9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti phospho gsk3β ser9
    Rabbit Anti Phospho Gsk3β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho gsk3β ser9/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti phospho gsk3β ser9 - by Bioz Stars, 2024-06
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    phospho gsk 3β ser9 d85e12 xp rabbit mab antibodies  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc phospho gsk 3β ser9 d85e12 xp rabbit mab antibodies
    LGS inhibits LPS-induced neutrophil inflammatory infiltration by suppressing GSK-3β expression in zebrafish. 3dpf larvae yolk-microinjected with 0.5 mg/mL LPS or PBS, and then treated with DEX (5 μg/mL) or LGS (25, 50, 100 μg/mL) or TWS119 (30 μM) or Wortmannin (0.125 μg/mL). After 12 hpi, larvae were dehydrated and embedded in paraffin for H&E staining assay (A) , ×100); Representative fluorescence images (B) , red circle), Statistical analysis (C) and inflammatory grading scatter diagrams (D) of neutrophils in yolk were shown; The mRNA expression level of GSK-3β was determined by q-PCR (E) The protein expressions of p-GSK-3β <t>(Ser9)</t> and GSK-3β were measured by Western blot (F) . Data are presented as mean ± SEM, n = 3, LPS group vs. blank group, * p < 0.05; vs. LPS group, ### p < 0.0001, # p < 0.05; vs. LPS + LGS group, + p < 0.05.
    Phospho Gsk 3β Ser9 D85e12 Xp Rabbit Mab Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho gsk 3β ser9 d85e12 xp rabbit mab antibodies/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    phospho gsk 3β ser9 d85e12 xp rabbit mab antibodies - by Bioz Stars, 2024-06
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    1) Product Images from "Liang-Ge-San: a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β"

    Article Title: Liang-Ge-San: a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2023.1181319

    LGS inhibits LPS-induced neutrophil inflammatory infiltration by suppressing GSK-3β expression in zebrafish. 3dpf larvae yolk-microinjected with 0.5 mg/mL LPS or PBS, and then treated with DEX (5 μg/mL) or LGS (25, 50, 100 μg/mL) or TWS119 (30 μM) or Wortmannin (0.125 μg/mL). After 12 hpi, larvae were dehydrated and embedded in paraffin for H&E staining assay (A) , ×100); Representative fluorescence images (B) , red circle), Statistical analysis (C) and inflammatory grading scatter diagrams (D) of neutrophils in yolk were shown; The mRNA expression level of GSK-3β was determined by q-PCR (E) The protein expressions of p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (F) . Data are presented as mean ± SEM, n = 3, LPS group vs. blank group, * p < 0.05; vs. LPS group, ### p < 0.0001, # p < 0.05; vs. LPS + LGS group, + p < 0.05.
    Figure Legend Snippet: LGS inhibits LPS-induced neutrophil inflammatory infiltration by suppressing GSK-3β expression in zebrafish. 3dpf larvae yolk-microinjected with 0.5 mg/mL LPS or PBS, and then treated with DEX (5 μg/mL) or LGS (25, 50, 100 μg/mL) or TWS119 (30 μM) or Wortmannin (0.125 μg/mL). After 12 hpi, larvae were dehydrated and embedded in paraffin for H&E staining assay (A) , ×100); Representative fluorescence images (B) , red circle), Statistical analysis (C) and inflammatory grading scatter diagrams (D) of neutrophils in yolk were shown; The mRNA expression level of GSK-3β was determined by q-PCR (E) The protein expressions of p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (F) . Data are presented as mean ± SEM, n = 3, LPS group vs. blank group, * p < 0.05; vs. LPS group, ### p < 0.0001, # p < 0.05; vs. LPS + LGS group, + p < 0.05.

    Techniques Used: Expressing, Staining, Fluorescence, Western Blot

    Effect of LGS on LPS-induced macrophage cell polarization. RAW264.7 cells (M0) were pre-stimulated with 100 ng/mL LPS for 48 h to obtain M1 macrophages. LGS (62.5, 125, 250 μg/mL) or TWS119 (5 μM) was co-stimulated with LPS (1 μg/mL) for 48 h. TWS119 (2.5–12.5 μg/mL) showed no significant cytotoxicity in RAW264.7. Cell survival was detected by MTT assay (A) , * p < 0.05, vs. blank group). The mRNA expression level of GSK-3β, iNOS and MR were determined by q-PCR (B–D) . The protein expressions of iNOS, MR, p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (E) . Data are presented as mean ± SEM, n = 3, * p < 0.05, *** p < 0.0001, LGS group vs. blank group; # p < 0.05, ## p < 0.001, ### p < 0.0001, vs. LGS group.
    Figure Legend Snippet: Effect of LGS on LPS-induced macrophage cell polarization. RAW264.7 cells (M0) were pre-stimulated with 100 ng/mL LPS for 48 h to obtain M1 macrophages. LGS (62.5, 125, 250 μg/mL) or TWS119 (5 μM) was co-stimulated with LPS (1 μg/mL) for 48 h. TWS119 (2.5–12.5 μg/mL) showed no significant cytotoxicity in RAW264.7. Cell survival was detected by MTT assay (A) , * p < 0.05, vs. blank group). The mRNA expression level of GSK-3β, iNOS and MR were determined by q-PCR (B–D) . The protein expressions of iNOS, MR, p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (E) . Data are presented as mean ± SEM, n = 3, * p < 0.05, *** p < 0.0001, LGS group vs. blank group; # p < 0.05, ## p < 0.001, ### p < 0.0001, vs. LGS group.

    Techniques Used: MTT Assay, Expressing, Western Blot

    resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology
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    Cell Signaling Technology Inc rabbit polyclonal anti phospho synapsin 1 ser9
    Sema5A enhances the density of <t>synapsin</t> <t>1</t> clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also <xref ref-type=Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. " width="250" height="auto" />
    Rabbit Polyclonal Anti Phospho Synapsin 1 Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho gsk 3ß ser9
    Reagent resources and list of primers
    Rabbit Monoclonal Anti Phospho Gsk 3ß Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit anti phospho gsk3β ser9
    KEY RESOURCES TABLE
    Rabbit Anti Phospho Gsk3β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc resource source identifier antibodies phospho gsk 3b ser9 rabbit pab cell signaling technology
    KEY RESOURCES TABLE
    Resource Source Identifier Antibodies Phospho Gsk 3b Ser9 Rabbit Pab Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho gsk 3β ser9
    Antibody information.
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    Cell Signaling Technology Inc phospho gsk 3β ser9 d85e12 xp rabbit mab antibodies
    LGS inhibits LPS-induced neutrophil inflammatory infiltration by suppressing GSK-3β expression in zebrafish. 3dpf larvae yolk-microinjected with 0.5 mg/mL LPS or PBS, and then treated with DEX (5 μg/mL) or LGS (25, 50, 100 μg/mL) or TWS119 (30 μM) or Wortmannin (0.125 μg/mL). After 12 hpi, larvae were dehydrated and embedded in paraffin for H&E staining assay (A) , ×100); Representative fluorescence images (B) , red circle), Statistical analysis (C) and inflammatory grading scatter diagrams (D) of neutrophils in yolk were shown; The mRNA expression level of GSK-3β was determined by q-PCR (E) The protein expressions of p-GSK-3β <t>(Ser9)</t> and GSK-3β were measured by Western blot (F) . Data are presented as mean ± SEM, n = 3, LPS group vs. blank group, * p < 0.05; vs. LPS group, ### p < 0.0001, # p < 0.05; vs. LPS + LGS group, + p < 0.05.
    Phospho Gsk 3β Ser9 D85e12 Xp Rabbit Mab Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also <xref ref-type=Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. " width="100%" height="100%">

    Journal: iScience

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    doi: 10.1016/j.isci.2024.109638

    Figure Lengend Snippet: Sema5A enhances the density of synapsin 1 clusters without affecting synapsin 1 protein expression (A) Schematic illustrating gut explants cocultured with COS cells expressing Fc, Sema5A-Fc, Sema5A-S951C-Fc, or Sema5A-S956G-Fc. Transfected COS cells suspended in Matrigel were deposited in the culture well next to the explant and this coculture was maintained for 3 days. Protein expression was analyzed by western blotting for synapsin 1 (syn1) (B) and the axonal protein β3-tubulin (C). The distribution of synapsin 1 was assessed by immunofluorescence (F) and the number and size of synapsin 1 clusters were measured (D and E). In (F), synapsin 1 clusters are shown by white arrows. (See also Figure S3 ). Data are mean ± SEM. (western-blot analysis (B and C): n = 22 explants from 4 independent experiments, IHC (D and E) n = 18–23 explants from 6 independent experiments); ∗ p < 0.05,∗∗ p < 0.01, Kruskal-Wallis test, supplemented by Dunn’s post-hoc test.

    Article Snippet: Rabbit polyclonal anti-phospho-synapsin 1 (Ser9) , Cell Signaling , Cat: CST2311.

    Techniques: Expressing, Transfection, Western Blot, Immunofluorescence

    Sema5A increases enteric neurons synapsin 1 phosphorylation at site Ser-603 Western blot analysis of synapsin 1 phosphorylation at site Ser603, Ser9 and Ser62/67 from enteric neuron cultures exposed for 3 days to control Fc, Sema5A-Fc, or Sema5A-S956G-Fc. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Journal: iScience

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    doi: 10.1016/j.isci.2024.109638

    Figure Lengend Snippet: Sema5A increases enteric neurons synapsin 1 phosphorylation at site Ser-603 Western blot analysis of synapsin 1 phosphorylation at site Ser603, Ser9 and Ser62/67 from enteric neuron cultures exposed for 3 days to control Fc, Sema5A-Fc, or Sema5A-S956G-Fc. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Article Snippet: Rabbit polyclonal anti-phospho-synapsin 1 (Ser9) , Cell Signaling , Cat: CST2311.

    Techniques: Western Blot

    KCl-induced synaptic vesicle exocytosis is reduced by Sema5A in association with a decrease in synapsin 1 phosphorylation at site Ser-603 and Ser-9 (A) From left to right, merged picture of FM1-43 fluorescence and brightfield microscopy at t0s. Representative images of enteric neurons labeled with FM1-43 at t0s and t4 min after 90 mM KCl stimulation. (B) FM1-43 fluorescence decreases over 4 min exposure to 90 mM KCl. (C) Remaining fluorescence was measured as destaining fraction, after 4 min exposure to KCl. Destaining fraction of FM1-43 fluorescence is expressed as 1-(Fluo t4 min/Fluo at t0). Data are mean ± SEM. ( n = 60 clusters per culture well, from 12 culture wells from 4 independent experiments); ∗∗∗ p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test. (D) Neurons were depolarized by 1 min exposure to 90 mM KCl and collected for western blot analysis and assessment of synapsin 1 phosphorylation at site Ser-603, Ser-9 and Ser-62/67. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Journal: iScience

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    doi: 10.1016/j.isci.2024.109638

    Figure Lengend Snippet: KCl-induced synaptic vesicle exocytosis is reduced by Sema5A in association with a decrease in synapsin 1 phosphorylation at site Ser-603 and Ser-9 (A) From left to right, merged picture of FM1-43 fluorescence and brightfield microscopy at t0s. Representative images of enteric neurons labeled with FM1-43 at t0s and t4 min after 90 mM KCl stimulation. (B) FM1-43 fluorescence decreases over 4 min exposure to 90 mM KCl. (C) Remaining fluorescence was measured as destaining fraction, after 4 min exposure to KCl. Destaining fraction of FM1-43 fluorescence is expressed as 1-(Fluo t4 min/Fluo at t0). Data are mean ± SEM. ( n = 60 clusters per culture well, from 12 culture wells from 4 independent experiments); ∗∗∗ p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test. (D) Neurons were depolarized by 1 min exposure to 90 mM KCl and collected for western blot analysis and assessment of synapsin 1 phosphorylation at site Ser-603, Ser-9 and Ser-62/67. ∗ p < 0.05 Kruskal-Wallis test, supplemented by Dunn’s post-hoc test. Data are mean ± SEM, n = 8 cultures from 2 independent experiments.

    Article Snippet: Rabbit polyclonal anti-phospho-synapsin 1 (Ser9) , Cell Signaling , Cat: CST2311.

    Techniques: Fluorescence, Microscopy, Labeling, Western Blot

    Enteric neurons exposed to Sema5A for 5 min show increased CaMKII phosphorylation and synapsin 1 phosphorylation at site Ser603 Enteric neurons were treated for 5 min or 30 min with Fc fragment or Sema5A-Fc (4.5 μM). Western blot for phosphorylated CaMKII at Tyr286 (A), phosphorylated ERK at Thr202/Tyr204 (B), and synapsin 1 at Ser-603 (C). ∗ p < 0.05 Mann-Whitney test for each time group. Data are mean ± SEM, n = 12 to 16 cultures from 3 to 4 independent experiments.

    Journal: iScience

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    doi: 10.1016/j.isci.2024.109638

    Figure Lengend Snippet: Enteric neurons exposed to Sema5A for 5 min show increased CaMKII phosphorylation and synapsin 1 phosphorylation at site Ser603 Enteric neurons were treated for 5 min or 30 min with Fc fragment or Sema5A-Fc (4.5 μM). Western blot for phosphorylated CaMKII at Tyr286 (A), phosphorylated ERK at Thr202/Tyr204 (B), and synapsin 1 at Ser-603 (C). ∗ p < 0.05 Mann-Whitney test for each time group. Data are mean ± SEM, n = 12 to 16 cultures from 3 to 4 independent experiments.

    Article Snippet: Rabbit polyclonal anti-phospho-synapsin 1 (Ser9) , Cell Signaling , Cat: CST2311.

    Techniques: Western Blot, MANN-WHITNEY

    Journal: iScience

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    doi: 10.1016/j.isci.2024.109638

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-phospho-synapsin 1 (Ser9) , Cell Signaling , Cat: CST2311.

    Techniques: Recombinant, Western Blot, Mutagenesis, Bicinchoninic Acid Protein Assay, Software, Membrane

    Reagent resources and list of primers

    Journal: The Journal of Biological Chemistry

    Article Title: Disruption of lysosomal nutrient sensing scaffold contributes to pathogenesis of a fatal neurodegenerative lysosomal storage disease

    doi: 10.1016/j.jbc.2024.105641

    Figure Lengend Snippet: Reagent resources and list of primers

    Article Snippet: Rabbit monoclonal anti-phospho-GSK-3ß (Ser9) (D85E12) , Cell signaling , Cat#5558; RRID: AB_.

    Techniques: Electron Microscopy, Recombinant, Protease Inhibitor, Staining, Isolation, Membrane, Enzyme-linked Immunosorbent Assay, Activation Assay, Mutagenesis

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration

    doi: 10.1016/j.celrep.2023.112647

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit anti-phospho-GSK3β(Ser9) , Cell Signaling Technology , Cat# 5558; RRID: AB_10013750.

    Techniques: Recombinant, Staining, SYBR Green Assay, Lysis, Protease Inhibitor, Generated, Transgenic Assay, Software

    Antibody information.

    Journal: Frontiers in Aging Neuroscience

    Article Title: The γ-Adducin 1–357 fragment promotes tau pathology

    doi: 10.3389/fnagi.2023.1241750

    Figure Lengend Snippet: Antibody information.

    Article Snippet: Rabbit monoclonal anti-phospho-GSK-3β (Ser9) , Cell Signaling Technology , 9322S , 1:1000 for Western blot.

    Techniques: Western Blot, Immunofluorescence

    GSK-3β mediates tau phosphorylation induced by γ-adducin 1–357. (A,B) Western blot of the levels of CDK5 (Cyclin-dependent kinase 5), Akt (Protein kinase B), and GSK-3β (Glycogen synthase kinase-3β) in primary neurons. (C,D) Western blot analysis of the levels of GSK3β, p-tau Ser396 (phosphorylated tau at Ser396), and total tau in primary neurons with or without TDZD-8 (specific kinase inhibitor TDZD-8). Data were presented as mean ± SD, n = 6 independent experiments. n.s. not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by one-way ANOVA.

    Journal: Frontiers in Aging Neuroscience

    Article Title: The γ-Adducin 1–357 fragment promotes tau pathology

    doi: 10.3389/fnagi.2023.1241750

    Figure Lengend Snippet: GSK-3β mediates tau phosphorylation induced by γ-adducin 1–357. (A,B) Western blot of the levels of CDK5 (Cyclin-dependent kinase 5), Akt (Protein kinase B), and GSK-3β (Glycogen synthase kinase-3β) in primary neurons. (C,D) Western blot analysis of the levels of GSK3β, p-tau Ser396 (phosphorylated tau at Ser396), and total tau in primary neurons with or without TDZD-8 (specific kinase inhibitor TDZD-8). Data were presented as mean ± SD, n = 6 independent experiments. n.s. not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by one-way ANOVA.

    Article Snippet: Rabbit monoclonal anti-phospho-GSK-3β (Ser9) , Cell Signaling Technology , 9322S , 1:1000 for Western blot.

    Techniques: Western Blot

    LGS inhibits LPS-induced neutrophil inflammatory infiltration by suppressing GSK-3β expression in zebrafish. 3dpf larvae yolk-microinjected with 0.5 mg/mL LPS or PBS, and then treated with DEX (5 μg/mL) or LGS (25, 50, 100 μg/mL) or TWS119 (30 μM) or Wortmannin (0.125 μg/mL). After 12 hpi, larvae were dehydrated and embedded in paraffin for H&E staining assay (A) , ×100); Representative fluorescence images (B) , red circle), Statistical analysis (C) and inflammatory grading scatter diagrams (D) of neutrophils in yolk were shown; The mRNA expression level of GSK-3β was determined by q-PCR (E) The protein expressions of p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (F) . Data are presented as mean ± SEM, n = 3, LPS group vs. blank group, * p < 0.05; vs. LPS group, ### p < 0.0001, # p < 0.05; vs. LPS + LGS group, + p < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Liang-Ge-San: a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β

    doi: 10.3389/fphar.2023.1181319

    Figure Lengend Snippet: LGS inhibits LPS-induced neutrophil inflammatory infiltration by suppressing GSK-3β expression in zebrafish. 3dpf larvae yolk-microinjected with 0.5 mg/mL LPS or PBS, and then treated with DEX (5 μg/mL) or LGS (25, 50, 100 μg/mL) or TWS119 (30 μM) or Wortmannin (0.125 μg/mL). After 12 hpi, larvae were dehydrated and embedded in paraffin for H&E staining assay (A) , ×100); Representative fluorescence images (B) , red circle), Statistical analysis (C) and inflammatory grading scatter diagrams (D) of neutrophils in yolk were shown; The mRNA expression level of GSK-3β was determined by q-PCR (E) The protein expressions of p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (F) . Data are presented as mean ± SEM, n = 3, LPS group vs. blank group, * p < 0.05; vs. LPS group, ### p < 0.0001, # p < 0.05; vs. LPS + LGS group, + p < 0.05.

    Article Snippet: Anti-Mannose Receptor antibodies were purchased from Abcam. iNOS (D6B6S) Rabbit mAb, GSK-3β (D5C5Z) XP ® Rabbit mAb and Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb antibodies were purchased from Cell Signaling Technology.

    Techniques: Expressing, Staining, Fluorescence, Western Blot

    Effect of LGS on LPS-induced macrophage cell polarization. RAW264.7 cells (M0) were pre-stimulated with 100 ng/mL LPS for 48 h to obtain M1 macrophages. LGS (62.5, 125, 250 μg/mL) or TWS119 (5 μM) was co-stimulated with LPS (1 μg/mL) for 48 h. TWS119 (2.5–12.5 μg/mL) showed no significant cytotoxicity in RAW264.7. Cell survival was detected by MTT assay (A) , * p < 0.05, vs. blank group). The mRNA expression level of GSK-3β, iNOS and MR were determined by q-PCR (B–D) . The protein expressions of iNOS, MR, p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (E) . Data are presented as mean ± SEM, n = 3, * p < 0.05, *** p < 0.0001, LGS group vs. blank group; # p < 0.05, ## p < 0.001, ### p < 0.0001, vs. LGS group.

    Journal: Frontiers in Pharmacology

    Article Title: Liang-Ge-San: a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β

    doi: 10.3389/fphar.2023.1181319

    Figure Lengend Snippet: Effect of LGS on LPS-induced macrophage cell polarization. RAW264.7 cells (M0) were pre-stimulated with 100 ng/mL LPS for 48 h to obtain M1 macrophages. LGS (62.5, 125, 250 μg/mL) or TWS119 (5 μM) was co-stimulated with LPS (1 μg/mL) for 48 h. TWS119 (2.5–12.5 μg/mL) showed no significant cytotoxicity in RAW264.7. Cell survival was detected by MTT assay (A) , * p < 0.05, vs. blank group). The mRNA expression level of GSK-3β, iNOS and MR were determined by q-PCR (B–D) . The protein expressions of iNOS, MR, p-GSK-3β (Ser9) and GSK-3β were measured by Western blot (E) . Data are presented as mean ± SEM, n = 3, * p < 0.05, *** p < 0.0001, LGS group vs. blank group; # p < 0.05, ## p < 0.001, ### p < 0.0001, vs. LGS group.

    Article Snippet: Anti-Mannose Receptor antibodies were purchased from Abcam. iNOS (D6B6S) Rabbit mAb, GSK-3β (D5C5Z) XP ® Rabbit mAb and Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb antibodies were purchased from Cell Signaling Technology.

    Techniques: MTT Assay, Expressing, Western Blot