rabbit anti pha l antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories rabbit anti pha l antibody
    Electron micrographs of a <t>PHA-L+</t> type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.
    Rabbit Anti Pha L Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti pha l antibody/product/Vector Laboratories
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rabbit anti pha l antibody - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA"

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2011.03.027

    Electron micrographs of a PHA-L+ type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.
    Figure Legend Snippet: Electron micrographs of a PHA-L+ type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.

    Techniques Used:

    PHA-L injections into the basal forebrain mainly label axons in the basolateral amygdalar nucleus. (A) Representative dual injections of PHA-L into the caudal basal forebrain (BF) that involved the ventral pallidum (VP) and substantia innominata (SI)
    Figure Legend Snippet: PHA-L injections into the basal forebrain mainly label axons in the basolateral amygdalar nucleus. (A) Representative dual injections of PHA-L into the caudal basal forebrain (BF) that involved the ventral pallidum (VP) and substantia innominata (SI)

    Techniques Used:

    PHA-L labeled axons in the basolateral nucleus. (A) Photomontage of PHA-L-labeled type 1 axons (black) innervating PV+ interneurons (brown) in the BLa in a field dominated by these axons. Varicosities of type 1 axons are often large and clustered, and
    Figure Legend Snippet: PHA-L labeled axons in the basolateral nucleus. (A) Photomontage of PHA-L-labeled type 1 axons (black) innervating PV+ interneurons (brown) in the BLa in a field dominated by these axons. Varicosities of type 1 axons are often large and clustered, and

    Techniques Used: Labeling

    Triple immunofluorescence labeling for PHA-L, VGAT, and neuronal markers in the BLa. PHA-L+ axons are red, VGAT is green, and neuronal markers (CB in A–E; CaMK in F) are blue. Yellow indicates colocalization of PHA-L and VGAT (i.e. GABAergic axons).
    Figure Legend Snippet: Triple immunofluorescence labeling for PHA-L, VGAT, and neuronal markers in the BLa. PHA-L+ axons are red, VGAT is green, and neuronal markers (CB in A–E; CaMK in F) are blue. Yellow indicates colocalization of PHA-L and VGAT (i.e. GABAergic axons).

    Techniques Used: Immunofluorescence, Labeling

    Synaptic contacts from type 1 PHA-L+ terminals with the perikarya of a PV-immunoreactive interneuron and an unlabeled putative pyramidal neuron (inset in B). (A, B) Schematic drawings, at two levels, of a single parvalbumin-immunoreactive interneuronal
    Figure Legend Snippet: Synaptic contacts from type 1 PHA-L+ terminals with the perikarya of a PV-immunoreactive interneuron and an unlabeled putative pyramidal neuron (inset in B). (A, B) Schematic drawings, at two levels, of a single parvalbumin-immunoreactive interneuronal

    Techniques Used:

    Synaptic contacts from type 1 PHA-L+ terminals onto the proximal dendrites of a PV+ interneuron (inset in B). (A) The proximal end of the lower dendrite of the PV+ interneuron (PVd) receives synaptic input (arrow) from a large PHA-L+ terminal (t1: terminal
    Figure Legend Snippet: Synaptic contacts from type 1 PHA-L+ terminals onto the proximal dendrites of a PV+ interneuron (inset in B). (A) The proximal end of the lower dendrite of the PV+ interneuron (PVd) receives synaptic input (arrow) from a large PHA-L+ terminal (t1: terminal

    Techniques Used:

    (A, B) Electron micrographs of PHA-L+ terminals 2 and 3 (t2, t3) shown in , making synaptic contacts (arrows) onto the PV + interneuron (PVpk) and putative pyramidal neuron (PNpk). Scale bar: 0.5 µm.
    Figure Legend Snippet: (A, B) Electron micrographs of PHA-L+ terminals 2 and 3 (t2, t3) shown in , making synaptic contacts (arrows) onto the PV + interneuron (PVpk) and putative pyramidal neuron (PNpk). Scale bar: 0.5 µm.

    Techniques Used:

    2) Product Images from "Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala"

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2011.12.036

    Photomicrographs of PHA-L-labeled mPFC projections (black) to the left amygdala in cases R37 (A1 and A2), R38 (B1 and B2), and R39 (C1 and C2) at two rostrocaudal (bregma −2.2: A1, B1, C1; bregma −2.6: A2, B2, C2) (lateral is to the right).
    Figure Legend Snippet: Photomicrographs of PHA-L-labeled mPFC projections (black) to the left amygdala in cases R37 (A1 and A2), R38 (B1 and B2), and R39 (C1 and C2) at two rostrocaudal (bregma −2.2: A1, B1, C1; bregma −2.6: A2, B2, C2) (lateral is to the right).

    Techniques Used: Labeling

    Photomicrograph (left panel) and camera lucida drawing (right panel) of PHA-L-labeled infralimbic projections (black) to the ipsilateral amygdala in case P-39 (lateral is to the left). The section in the left panel was counterstained with pyronin Y. Asterisks
    Figure Legend Snippet: Photomicrograph (left panel) and camera lucida drawing (right panel) of PHA-L-labeled infralimbic projections (black) to the ipsilateral amygdala in case P-39 (lateral is to the left). The section in the left panel was counterstained with pyronin Y. Asterisks

    Techniques Used: Labeling

    Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.6. MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense concentration of IL fibers in the amygdalostriatal
    Figure Legend Snippet: Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.6. MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense concentration of IL fibers in the amygdalostriatal

    Techniques Used: Labeling, Concentration Assay

    Gray scale digital micrographs of a PHA-L injection into the infralimbic cortex (IL) in a section that was lightly Nissl-stained with pyronin Y (injection R36Rt; compare with ). (A) Low power photomicrograph. (B) Higher power photomicrograph of
    Figure Legend Snippet: Gray scale digital micrographs of a PHA-L injection into the infralimbic cortex (IL) in a section that was lightly Nissl-stained with pyronin Y (injection R36Rt; compare with ). (A) Low power photomicrograph. (B) Higher power photomicrograph of

    Techniques Used: Injection, Staining

    Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.2 (lateral is to the right). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a moderate innervation
    Figure Legend Snippet: Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.2 (lateral is to the right). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a moderate innervation

    Techniques Used: Labeling

    Photomicrographs illustrating PHA-L-labeled infralimbic/prelimbic projections (black) to the right amygdala in a case with an injection site that involved both IL and PL (R40). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense
    Figure Legend Snippet: Photomicrographs illustrating PHA-L-labeled infralimbic/prelimbic projections (black) to the right amygdala in a case with an injection site that involved both IL and PL (R40). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense

    Techniques Used: Labeling, Injection

    Camera lucida drawings of PHA-L labeled axons in the right amygdala in case R36. (A) Drawing of the PHA-L labeled axons in the section shown in (lateral is to the right). Although almost all of these axons exhibited varicosities, they are not
    Figure Legend Snippet: Camera lucida drawings of PHA-L labeled axons in the right amygdala in case R36. (A) Drawing of the PHA-L labeled axons in the section shown in (lateral is to the right). Although almost all of these axons exhibited varicosities, they are not

    Techniques Used: Labeling

    3) Product Images from "POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA"

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2011.03.027

    Electron micrographs of a PHA-L+ type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.
    Figure Legend Snippet: Electron micrographs of a PHA-L+ type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.

    Techniques Used:

    PHA-L injections into the basal forebrain mainly label axons in the basolateral amygdalar nucleus. (A) Representative dual injections of PHA-L into the caudal basal forebrain (BF) that involved the ventral pallidum (VP) and substantia innominata (SI)
    Figure Legend Snippet: PHA-L injections into the basal forebrain mainly label axons in the basolateral amygdalar nucleus. (A) Representative dual injections of PHA-L into the caudal basal forebrain (BF) that involved the ventral pallidum (VP) and substantia innominata (SI)

    Techniques Used:

    PHA-L labeled axons in the basolateral nucleus. (A) Photomontage of PHA-L-labeled type 1 axons (black) innervating PV+ interneurons (brown) in the BLa in a field dominated by these axons. Varicosities of type 1 axons are often large and clustered, and
    Figure Legend Snippet: PHA-L labeled axons in the basolateral nucleus. (A) Photomontage of PHA-L-labeled type 1 axons (black) innervating PV+ interneurons (brown) in the BLa in a field dominated by these axons. Varicosities of type 1 axons are often large and clustered, and

    Techniques Used: Labeling

    Triple immunofluorescence labeling for PHA-L, VGAT, and neuronal markers in the BLa. PHA-L+ axons are red, VGAT is green, and neuronal markers (CB in A–E; CaMK in F) are blue. Yellow indicates colocalization of PHA-L and VGAT (i.e. GABAergic axons).
    Figure Legend Snippet: Triple immunofluorescence labeling for PHA-L, VGAT, and neuronal markers in the BLa. PHA-L+ axons are red, VGAT is green, and neuronal markers (CB in A–E; CaMK in F) are blue. Yellow indicates colocalization of PHA-L and VGAT (i.e. GABAergic axons).

    Techniques Used: Immunofluorescence, Labeling

    Synaptic contacts from type 1 PHA-L+ terminals with the perikarya of a PV-immunoreactive interneuron and an unlabeled putative pyramidal neuron (inset in B). (A, B) Schematic drawings, at two levels, of a single parvalbumin-immunoreactive interneuronal
    Figure Legend Snippet: Synaptic contacts from type 1 PHA-L+ terminals with the perikarya of a PV-immunoreactive interneuron and an unlabeled putative pyramidal neuron (inset in B). (A, B) Schematic drawings, at two levels, of a single parvalbumin-immunoreactive interneuronal

    Techniques Used:

    Synaptic contacts from type 1 PHA-L+ terminals onto the proximal dendrites of a PV+ interneuron (inset in B). (A) The proximal end of the lower dendrite of the PV+ interneuron (PVd) receives synaptic input (arrow) from a large PHA-L+ terminal (t1: terminal
    Figure Legend Snippet: Synaptic contacts from type 1 PHA-L+ terminals onto the proximal dendrites of a PV+ interneuron (inset in B). (A) The proximal end of the lower dendrite of the PV+ interneuron (PVd) receives synaptic input (arrow) from a large PHA-L+ terminal (t1: terminal

    Techniques Used:

    (A, B) Electron micrographs of PHA-L+ terminals 2 and 3 (t2, t3) shown in , making synaptic contacts (arrows) onto the PV + interneuron (PVpk) and putative pyramidal neuron (PNpk). Scale bar: 0.5 µm.
    Figure Legend Snippet: (A, B) Electron micrographs of PHA-L+ terminals 2 and 3 (t2, t3) shown in , making synaptic contacts (arrows) onto the PV + interneuron (PVpk) and putative pyramidal neuron (PNpk). Scale bar: 0.5 µm.

    Techniques Used:

    4) Product Images from "Dopaminergic and glutamatergic microdomains within a subset of rodent mesoaccumbens axons"

    Article Title: Dopaminergic and glutamatergic microdomains within a subset of rodent mesoaccumbens axons

    Journal: Nature neuroscience

    doi: 10.1038/nn.3945

    Mesoaccumbens neurons establish either VGluT2-asymmetric synapses or TH-symmetric synapses (wild type rats) (a) VTA inputs to nAcc identified by PHA-L- or mCherry-immunoreactivity (IR). VTA neurons were tagged with PHA-L (n = 3) or AAV5-CaMKII-ChR2-mCherry (n = 2). Within the nAcc, PHA-L-IR axon terminals make asymmetric or symmetric synapses on dendrites or dendritic spines. (b) nAcc axon terminal 1 (AT 1 , green outline) co-expressing PHA-L-IR (scattered dark material) and VGluT2-IR (gold particles, green arrowhead) makes an asymmetric synapse (green arrow) with a dendrite (De, orange outline). AT 2 (yellow outline) contains only VGluT2-IR. (c) nAcc AT 1 (blue outline) co-expressing PHA-L-IR (scattered dark material) and TH-IR (gold particles, blue arrowhead) makes a symmetric synapse (blue arrow) on the side of a dendritic spine (sp, orange outline) with spine apparatus (sa). The head of this dendritic spine also makes an asymmetric synapse (green arrow) with the unlabeled AT 2 (green outline). AT 3 (yellow outline) has only TH-IR. (d-f) Triad arrangement in which ATs from VTA neurons are converging on the same dendritic spine and establishing different types of synapses. Serial sections of two ATs from VTA neurons expressing mCherry-IR (scattered dark material) under the control of the CaMKII promoter (d and e). Both ATs are making synapses on the same dendritic spine (sp, orange outline); AT 1 (green outline) makes an asymmetric synapse (green arrow) on the head of the spine and AT 2 (blue outline) makes a symmetric synapse (blue arrow) on the side of the same dendritic spine. Bars: (b, c, d, and e) 200 nm.
    Figure Legend Snippet: Mesoaccumbens neurons establish either VGluT2-asymmetric synapses or TH-symmetric synapses (wild type rats) (a) VTA inputs to nAcc identified by PHA-L- or mCherry-immunoreactivity (IR). VTA neurons were tagged with PHA-L (n = 3) or AAV5-CaMKII-ChR2-mCherry (n = 2). Within the nAcc, PHA-L-IR axon terminals make asymmetric or symmetric synapses on dendrites or dendritic spines. (b) nAcc axon terminal 1 (AT 1 , green outline) co-expressing PHA-L-IR (scattered dark material) and VGluT2-IR (gold particles, green arrowhead) makes an asymmetric synapse (green arrow) with a dendrite (De, orange outline). AT 2 (yellow outline) contains only VGluT2-IR. (c) nAcc AT 1 (blue outline) co-expressing PHA-L-IR (scattered dark material) and TH-IR (gold particles, blue arrowhead) makes a symmetric synapse (blue arrow) on the side of a dendritic spine (sp, orange outline) with spine apparatus (sa). The head of this dendritic spine also makes an asymmetric synapse (green arrow) with the unlabeled AT 2 (green outline). AT 3 (yellow outline) has only TH-IR. (d-f) Triad arrangement in which ATs from VTA neurons are converging on the same dendritic spine and establishing different types of synapses. Serial sections of two ATs from VTA neurons expressing mCherry-IR (scattered dark material) under the control of the CaMKII promoter (d and e). Both ATs are making synapses on the same dendritic spine (sp, orange outline); AT 1 (green outline) makes an asymmetric synapse (green arrow) on the head of the spine and AT 2 (blue outline) makes a symmetric synapse (blue arrow) on the side of the same dendritic spine. Bars: (b, c, d, and e) 200 nm.

    Techniques Used: Expressing

    5) Product Images from "Limited Convergence of Rhinal Cortical and Dopaminergic Inputs in the Rat Basolateral Amygdala: An Ultrastructural Analysis"

    Article Title: Limited Convergence of Rhinal Cortical and Dopaminergic Inputs in the Rat Basolateral Amygdala: An Ultrastructural Analysis

    Journal: Brain research

    doi: 10.1016/j.brainres.2010.03.062

    Photomicrographs depicting the results of PHA-L injections into the rhinal cortices (RCx). A) Photomicrograph of a representative injection into the perirhinal (PR) and dorsolateral entorhinal (DLEnt) cortices in case R33Rt. The effective injection site
    Figure Legend Snippet: Photomicrographs depicting the results of PHA-L injections into the rhinal cortices (RCx). A) Photomicrograph of a representative injection into the perirhinal (PR) and dorsolateral entorhinal (DLEnt) cortices in case R33Rt. The effective injection site

    Techniques Used: Injection

    Electron micrographs of the posterior subdivision of the basolateral amygdalar nucleus (BLp) immunostained for PHA-L (to identify RCx terminals) and TH (to identify DA terminals). TH+ terminals containing diffuse DAB reaction product are frequently found
    Figure Legend Snippet: Electron micrographs of the posterior subdivision of the basolateral amygdalar nucleus (BLp) immunostained for PHA-L (to identify RCx terminals) and TH (to identify DA terminals). TH+ terminals containing diffuse DAB reaction product are frequently found

    Techniques Used:

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    Vector Laboratories rabbit anti pha l antibody
    Electron micrographs of a <t>PHA-L+</t> type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.
    Rabbit Anti Pha L Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti pha l antibody/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti pha l antibody - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

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    Electron micrographs of a PHA-L+ type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: Electron micrographs of a PHA-L+ type 1 terminal (T1t) making synaptic contact (arrowhead) with the base of a proximal dendrite (den) emerging from an unlabeled putative pyramidal cell perikaryon (Pyr, pk). Scale bar: 0.5 µm.

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques:

    PHA-L injections into the basal forebrain mainly label axons in the basolateral amygdalar nucleus. (A) Representative dual injections of PHA-L into the caudal basal forebrain (BF) that involved the ventral pallidum (VP) and substantia innominata (SI)

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: PHA-L injections into the basal forebrain mainly label axons in the basolateral amygdalar nucleus. (A) Representative dual injections of PHA-L into the caudal basal forebrain (BF) that involved the ventral pallidum (VP) and substantia innominata (SI)

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques:

    PHA-L labeled axons in the basolateral nucleus. (A) Photomontage of PHA-L-labeled type 1 axons (black) innervating PV+ interneurons (brown) in the BLa in a field dominated by these axons. Varicosities of type 1 axons are often large and clustered, and

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: PHA-L labeled axons in the basolateral nucleus. (A) Photomontage of PHA-L-labeled type 1 axons (black) innervating PV+ interneurons (brown) in the BLa in a field dominated by these axons. Varicosities of type 1 axons are often large and clustered, and

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques: Labeling

    Triple immunofluorescence labeling for PHA-L, VGAT, and neuronal markers in the BLa. PHA-L+ axons are red, VGAT is green, and neuronal markers (CB in A–E; CaMK in F) are blue. Yellow indicates colocalization of PHA-L and VGAT (i.e. GABAergic axons).

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: Triple immunofluorescence labeling for PHA-L, VGAT, and neuronal markers in the BLa. PHA-L+ axons are red, VGAT is green, and neuronal markers (CB in A–E; CaMK in F) are blue. Yellow indicates colocalization of PHA-L and VGAT (i.e. GABAergic axons).

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques: Immunofluorescence, Labeling

    Synaptic contacts from type 1 PHA-L+ terminals with the perikarya of a PV-immunoreactive interneuron and an unlabeled putative pyramidal neuron (inset in B). (A, B) Schematic drawings, at two levels, of a single parvalbumin-immunoreactive interneuronal

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: Synaptic contacts from type 1 PHA-L+ terminals with the perikarya of a PV-immunoreactive interneuron and an unlabeled putative pyramidal neuron (inset in B). (A, B) Schematic drawings, at two levels, of a single parvalbumin-immunoreactive interneuronal

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques:

    Synaptic contacts from type 1 PHA-L+ terminals onto the proximal dendrites of a PV+ interneuron (inset in B). (A) The proximal end of the lower dendrite of the PV+ interneuron (PVd) receives synaptic input (arrow) from a large PHA-L+ terminal (t1: terminal

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: Synaptic contacts from type 1 PHA-L+ terminals onto the proximal dendrites of a PV+ interneuron (inset in B). (A) The proximal end of the lower dendrite of the PV+ interneuron (PVd) receives synaptic input (arrow) from a large PHA-L+ terminal (t1: terminal

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques:

    (A, B) Electron micrographs of PHA-L+ terminals 2 and 3 (t2, t3) shown in , making synaptic contacts (arrows) onto the PV + interneuron (PVpk) and putative pyramidal neuron (PNpk). Scale bar: 0.5 µm.

    Journal: Neuroscience

    Article Title: POSTSYNAPTIC TARGETS OF GABAERGIC BASAL FOREBRAIN PROJECTIONS TO THE BASOLATERAL AMYGDALA

    doi: 10.1016/j.neuroscience.2011.03.027

    Figure Lengend Snippet: (A, B) Electron micrographs of PHA-L+ terminals 2 and 3 (t2, t3) shown in , making synaptic contacts (arrows) onto the PV + interneuron (PVpk) and putative pyramidal neuron (PNpk). Scale bar: 0.5 µm.

    Article Snippet: Sections through the injection sites and amygdala in PHA-L injected rats were incubated in a rabbit anti-PHA-L antibody (1: 1000; Vector Laboratories Inc.) for 16 h at 4 °C and then processed for the avidin-biotin immunoperoxidase technique using a rabbit Vectastain ABC kit (Vector Laboratories Inc.).

    Techniques:

    Photomicrographs of PHA-L-labeled mPFC projections (black) to the left amygdala in cases R37 (A1 and A2), R38 (B1 and B2), and R39 (C1 and C2) at two rostrocaudal (bregma −2.2: A1, B1, C1; bregma −2.6: A2, B2, C2) (lateral is to the right).

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Photomicrographs of PHA-L-labeled mPFC projections (black) to the left amygdala in cases R37 (A1 and A2), R38 (B1 and B2), and R39 (C1 and C2) at two rostrocaudal (bregma −2.2: A1, B1, C1; bregma −2.6: A2, B2, C2) (lateral is to the right).

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Labeling

    Photomicrograph (left panel) and camera lucida drawing (right panel) of PHA-L-labeled infralimbic projections (black) to the ipsilateral amygdala in case P-39 (lateral is to the left). The section in the left panel was counterstained with pyronin Y. Asterisks

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Photomicrograph (left panel) and camera lucida drawing (right panel) of PHA-L-labeled infralimbic projections (black) to the ipsilateral amygdala in case P-39 (lateral is to the left). The section in the left panel was counterstained with pyronin Y. Asterisks

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Labeling

    Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.6. MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense concentration of IL fibers in the amygdalostriatal

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.6. MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense concentration of IL fibers in the amygdalostriatal

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Labeling, Concentration Assay

    Gray scale digital micrographs of a PHA-L injection into the infralimbic cortex (IL) in a section that was lightly Nissl-stained with pyronin Y (injection R36Rt; compare with ). (A) Low power photomicrograph. (B) Higher power photomicrograph of

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Gray scale digital micrographs of a PHA-L injection into the infralimbic cortex (IL) in a section that was lightly Nissl-stained with pyronin Y (injection R36Rt; compare with ). (A) Low power photomicrograph. (B) Higher power photomicrograph of

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Injection, Staining

    Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.2 (lateral is to the right). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a moderate innervation

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Photomicrographs illustrating PHA-L-labeled infralimbic projections (black) to the right amygdala in case R36 at bregma level −2.2 (lateral is to the right). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a moderate innervation

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Labeling

    Photomicrographs illustrating PHA-L-labeled infralimbic/prelimbic projections (black) to the right amygdala in a case with an injection site that involved both IL and PL (R40). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Photomicrographs illustrating PHA-L-labeled infralimbic/prelimbic projections (black) to the right amygdala in a case with an injection site that involved both IL and PL (R40). MOR-labeled ICNs are brown. (A) Low power photomicrograph showing a dense

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Labeling, Injection

    Camera lucida drawings of PHA-L labeled axons in the right amygdala in case R36. (A) Drawing of the PHA-L labeled axons in the section shown in (lateral is to the right). Although almost all of these axons exhibited varicosities, they are not

    Journal: Neuroscience

    Article Title: Medial Prefrontal Cortical Innervation of the Intercalated Nuclear Region of the Amygdala

    doi: 10.1016/j.neuroscience.2011.12.036

    Figure Lengend Snippet: Camera lucida drawings of PHA-L labeled axons in the right amygdala in case R36. (A) Drawing of the PHA-L labeled axons in the section shown in (lateral is to the right). Although almost all of these axons exhibited varicosities, they are not

    Article Snippet: A one in three series of sections through the amygdala were first incubated overnight at 4°C in a rabbit anti-PHA-L antibody (1:1000; Vector Laboratories, Burlingame, CA).

    Techniques: Labeling

    Mesoaccumbens neurons establish either VGluT2-asymmetric synapses or TH-symmetric synapses (wild type rats) (a) VTA inputs to nAcc identified by PHA-L- or mCherry-immunoreactivity (IR). VTA neurons were tagged with PHA-L (n = 3) or AAV5-CaMKII-ChR2-mCherry (n = 2). Within the nAcc, PHA-L-IR axon terminals make asymmetric or symmetric synapses on dendrites or dendritic spines. (b) nAcc axon terminal 1 (AT 1 , green outline) co-expressing PHA-L-IR (scattered dark material) and VGluT2-IR (gold particles, green arrowhead) makes an asymmetric synapse (green arrow) with a dendrite (De, orange outline). AT 2 (yellow outline) contains only VGluT2-IR. (c) nAcc AT 1 (blue outline) co-expressing PHA-L-IR (scattered dark material) and TH-IR (gold particles, blue arrowhead) makes a symmetric synapse (blue arrow) on the side of a dendritic spine (sp, orange outline) with spine apparatus (sa). The head of this dendritic spine also makes an asymmetric synapse (green arrow) with the unlabeled AT 2 (green outline). AT 3 (yellow outline) has only TH-IR. (d-f) Triad arrangement in which ATs from VTA neurons are converging on the same dendritic spine and establishing different types of synapses. Serial sections of two ATs from VTA neurons expressing mCherry-IR (scattered dark material) under the control of the CaMKII promoter (d and e). Both ATs are making synapses on the same dendritic spine (sp, orange outline); AT 1 (green outline) makes an asymmetric synapse (green arrow) on the head of the spine and AT 2 (blue outline) makes a symmetric synapse (blue arrow) on the side of the same dendritic spine. Bars: (b, c, d, and e) 200 nm.

    Journal: Nature neuroscience

    Article Title: Dopaminergic and glutamatergic microdomains within a subset of rodent mesoaccumbens axons

    doi: 10.1038/nn.3945

    Figure Lengend Snippet: Mesoaccumbens neurons establish either VGluT2-asymmetric synapses or TH-symmetric synapses (wild type rats) (a) VTA inputs to nAcc identified by PHA-L- or mCherry-immunoreactivity (IR). VTA neurons were tagged with PHA-L (n = 3) or AAV5-CaMKII-ChR2-mCherry (n = 2). Within the nAcc, PHA-L-IR axon terminals make asymmetric or symmetric synapses on dendrites or dendritic spines. (b) nAcc axon terminal 1 (AT 1 , green outline) co-expressing PHA-L-IR (scattered dark material) and VGluT2-IR (gold particles, green arrowhead) makes an asymmetric synapse (green arrow) with a dendrite (De, orange outline). AT 2 (yellow outline) contains only VGluT2-IR. (c) nAcc AT 1 (blue outline) co-expressing PHA-L-IR (scattered dark material) and TH-IR (gold particles, blue arrowhead) makes a symmetric synapse (blue arrow) on the side of a dendritic spine (sp, orange outline) with spine apparatus (sa). The head of this dendritic spine also makes an asymmetric synapse (green arrow) with the unlabeled AT 2 (green outline). AT 3 (yellow outline) has only TH-IR. (d-f) Triad arrangement in which ATs from VTA neurons are converging on the same dendritic spine and establishing different types of synapses. Serial sections of two ATs from VTA neurons expressing mCherry-IR (scattered dark material) under the control of the CaMKII promoter (d and e). Both ATs are making synapses on the same dendritic spine (sp, orange outline); AT 1 (green outline) makes an asymmetric synapse (green arrow) on the head of the spine and AT 2 (blue outline) makes a symmetric synapse (blue arrow) on the side of the same dendritic spine. Bars: (b, c, d, and e) 200 nm.

    Article Snippet: Sections were rinsed with 0.1 M PB (pH 7.3) and incubated with a blocking solution [4% bovine serum albumin (BSA) in PB supplemented with 0.3% Triton-X-100] for 1 h. Sections were then incubated with either rabbit anti-PHA-L antibody (AS-2300; Vector Labs, 1:1000 dilution), mouse anti-mCherry antibody (632543; Clontech Laboratories Inc., Mountain View, CA, 1:500 dilution), or mouse anti-Myc antibody (05-724; EMD Millipore, Billerica, MA, 1:500 dilution); in the blocking solution overnight at 4°C.

    Techniques: Expressing