Journal: Cell reports

Article Title: Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes

doi: 10.1016/j.celrep.2021.109490

Figure Lengend Snippet:

Article Snippet: Membranes were blocked at room temperature using mixture of 2.5% milk (Cell Signaling Technology (CST), MA, USA) and 2.5% BSA for 1h and incubated overnight at 4°C with rabbit anti-cleaved caspase-3 (#9664), rabbit anti-cleaved PARP (rat specific; #9545), rabbit anti-tubulin (#2146), rabbit anti-GAPDH (#2118), rabbit anti-β-actin (#4967), rabbit anti-GFP (#2956), rabbit anti-HA (#2367), rabbit anti-p4EBP1 (#2855), rabbit anti-pS6 (#4858), rabbit anti-pS6K (#9234), rabbit anti-Raptor (#2280), rabbit anti-MST1 (#3682), rabbit anti-AKT (#9272), rabbit anti-pAKT (#4058), rabbit anti-GST (#2625), rabbit anti-pGSK3 (#9336) (all CST), rabbit anti-PHLPP1 (RRID: AB_2750897 #22789-I-AP, Proteintech, IL, USA), rabbit anti-PHLPP2 (#A300–661A; Bethyl, TX, USA), rabbit anti-pMST1(T183) (#ab79199, Abcam, UK) and rabbit anti-GLUT2 antibody (#07–1402; Chemicon, CA, USA).

Techniques: Plasmid Preparation, Recombinant, Western Blot, Transfection, In Vivo, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, In Situ, Isolation, Knock-Out, Expressing, Mutagenesis, Software, Real-time Polymerase Chain Reaction

Journal: Cancer cell

Article Title: Synthetic Lethality of Combined Bcl-2 Inhibition and p53 Activation in AML: Mechanisms and Superior Antileukemic Efficacy

doi: 10.1016/j.ccell.2017.11.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies for immunoblotting were purchased from the following sources: p53 (sc-126), MDM2 (sc-5304), Bcl-2 (sc-7382), Mcl-1 (sc-819), Puma (sc-28226), ERK (sc-1647), N-Ras (sc-31) and GSK3 (sc-7291) from Santa Cruz Biotechnology (Dallas, TX, USA); pERK (T202/Y204, 4370), pMEK (S217/S221, 9121), MEK (4694), AKT (2920), pAKT(S473, 9271), pGSK3 (S21/S9, 8566), PARP-1 (9542), Bim (2819), Bak (12105), caspase-3 (9664), caspase-9 (9508), pMcl-1 (T163, 14765), B-Raf (9433), pB-Raf (S445, 2696), JNK (9252) and pJNK (T183Y185, 9255) from Cell Signaling Technology; Bax (B8554) and β-Actin (A2228) from Sigma-Aldrich; Noxa (ab13654) and pMcl-1 (T163S159, ab111574) from Abcam; p21 (OP64) from EMD Millipore (Darmstadt, Germany).

Techniques: Recombinant, Imaging, Staining, Mutagenesis, Transfection, shRNA, Negative Control, Plasmid Preparation, Software, In Vivo Imaging, Microscopy, Flow Cytometry

Journal: Cancer cell

Article Title: Synthetic Lethality of Combined Bcl-2 Inhibition and p53 Activation in AML: Mechanisms and Superior Antileukemic Efficacy

doi: 10.1016/j.ccell.2017.11.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-pGSK3 (S21/S9) (clone D17D2) , Cell Signaling Technology , Cat#8566; RRID: AB_10860069.

Techniques: Recombinant, Imaging, Staining, Mutagenesis, Transfection, shRNA, Negative Control, Plasmid Preparation, Software, In Vivo Imaging, Microscopy, Flow Cytometry