rabbit anti p2x3r  (Alomone Labs)


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    Alomone Labs rabbit anti p2x3r
    Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in <t>P2X3R-containing</t> dorsal root ganglia (DRG) neurons. (A) In saline control rats,
    Rabbit Anti P2x3r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti p2x3r/product/Alomone Labs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit anti p2x3r - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation"

    Article Title: Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000547

    Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats,
    Figure Legend Snippet: Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats,

    Techniques Used:

    Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found
    Figure Legend Snippet: Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found

    Techniques Used: Expressing

    2) Product Images from "Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation"

    Article Title: Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000547

    Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats, Go6976 (11.5 pmol = 4.35 ng in 50-μL saline) had no effect on α,β-meATP–elicited flinch activity. In complete Freund adjuvant–treated rats, Go6976 inhibited the enhanced α,β-meATP–induced flinch responses (n = 6; * P
    Figure Legend Snippet: Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats, Go6976 (11.5 pmol = 4.35 ng in 50-μL saline) had no effect on α,β-meATP–elicited flinch activity. In complete Freund adjuvant–treated rats, Go6976 inhibited the enhanced α,β-meATP–induced flinch responses (n = 6; * P

    Techniques Used: Activity Assay

    Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found both at the cell membrane and in the cytoplasm. P2X3Rs were colocalized with pPKCα in small or medium cells. Enlarged views of cells (indicated by arrows) are shown in the lower left corners. Lower: pPKCε was expressed in both small or medium and large cells, most contained punctate labels. pPKCε labels were colocalized with P2X3Rs in small or medium cells (bar scale = 25 μm).
    Figure Legend Snippet: Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found both at the cell membrane and in the cytoplasm. P2X3Rs were colocalized with pPKCα in small or medium cells. Enlarged views of cells (indicated by arrows) are shown in the lower left corners. Lower: pPKCε was expressed in both small or medium and large cells, most contained punctate labels. pPKCε labels were colocalized with P2X3Rs in small or medium cells (bar scale = 25 μm).

    Techniques Used: Expressing

    3) Product Images from "F-actin links Epac-PKC signaling to purinergic P2X3 receptor sensitization in dorsal root ganglia following inflammation"

    Article Title: F-actin links Epac-PKC signaling to purinergic P2X3 receptor sensitization in dorsal root ganglia following inflammation

    Journal: Molecular Pain

    doi: 10.1177/1744806916660557

    CPT increases the membrane expression of P2X3Rs in cultured DRGs. The P2X3R levels in total protein (Total), membrane protein (Mem), and cytoplasmic protein samples (Cytosol) before (Con) and after CPT (1 µM, 10 min) treatment were determined. Total, membrane, and cytosol protein samples were loaded onto gels. The expression of the ubiquitous membrane protein Na+/K+ ATPase was probed to indicate the membrane expressed protein levels in our samples. The expressions of β-tubulin used as sample loading controls were also indicated. CPT significantly increased P2X3Rs expressed in the cell membrane (total protein: CPT/Con = 1.19; membrane protein: CPT/Con = 1.93 and cytoplasmic protein: CPT/Con = 0.75). N = 3. * P
    Figure Legend Snippet: CPT increases the membrane expression of P2X3Rs in cultured DRGs. The P2X3R levels in total protein (Total), membrane protein (Mem), and cytoplasmic protein samples (Cytosol) before (Con) and after CPT (1 µM, 10 min) treatment were determined. Total, membrane, and cytosol protein samples were loaded onto gels. The expression of the ubiquitous membrane protein Na+/K+ ATPase was probed to indicate the membrane expressed protein levels in our samples. The expressions of β-tubulin used as sample loading controls were also indicated. CPT significantly increased P2X3Rs expressed in the cell membrane (total protein: CPT/Con = 1.19; membrane protein: CPT/Con = 1.93 and cytoplasmic protein: CPT/Con = 0.75). N = 3. * P

    Techniques Used: Cycling Probe Technology, Expressing, Cell Culture

    Epac control of the membrane expression of P2X3Rs in DRG neurons is F-actin mediated. (a) The membrane expression of P2X3Rs was examined in cultured DRG neurons using anti-ext-P2X3R antibody. The enlarged views of neurons (indicated by red arrows) are shown on the right side of each panel. Bars = 25 µm. (b) In the presence of CPT (1 µM), the percentage of cells expressing ext-P2X3R increased by 1.80 fold and the intensity of ext-P2X3R labels increased by 5.57 fold. The CPT-induced increase in membrane expressed P2X3Rs was significantly reduced by pre-incubating cells with the F-actin disrupter, LaA (1 µM, 60 min). A total of 1192 cells obtained from three experiments were used for the analyses. * P
    Figure Legend Snippet: Epac control of the membrane expression of P2X3Rs in DRG neurons is F-actin mediated. (a) The membrane expression of P2X3Rs was examined in cultured DRG neurons using anti-ext-P2X3R antibody. The enlarged views of neurons (indicated by red arrows) are shown on the right side of each panel. Bars = 25 µm. (b) In the presence of CPT (1 µM), the percentage of cells expressing ext-P2X3R increased by 1.80 fold and the intensity of ext-P2X3R labels increased by 5.57 fold. The CPT-induced increase in membrane expressed P2X3Rs was significantly reduced by pre-incubating cells with the F-actin disrupter, LaA (1 µM, 60 min). A total of 1192 cells obtained from three experiments were used for the analyses. * P

    Techniques Used: Expressing, Cell Culture, Cycling Probe Technology

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    Alomone Labs rabbit anti p2x3r
    Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in <t>P2X3R-containing</t> dorsal root ganglia (DRG) neurons. (A) In saline control rats,
    Rabbit Anti P2x3r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti p2x3r/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti p2x3r - by Bioz Stars, 2022-09
    93/100 stars
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    Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats,

    Journal: Pain

    Article Title: Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation

    doi: 10.1097/j.pain.0000000000000547

    Figure Lengend Snippet: Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats,

    Article Snippet: Antibodies used were mouse anti-Epac1, anti-Epac2 (1:1000; Cell Signaling, Danvers, MA), mouse anti-PKCε (1:1000; Santa Cruz), rabbit anti-pPKCε (1:1000; Santa Cruz), and rabbit anti-P2X3R (1:2000; Alomone Labs). β-Actin, probed with anti-β-actin (1:10,000; Chemicon, Temeculla, CA), or the neuronal marker, β-tubulin, probed with anti β-tubulin antibody (1:2000; Santa Cruz), was used as loading control.

    Techniques:

    Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found

    Journal: Pain

    Article Title: Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation

    doi: 10.1097/j.pain.0000000000000547

    Figure Lengend Snippet: Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found

    Article Snippet: Antibodies used were mouse anti-Epac1, anti-Epac2 (1:1000; Cell Signaling, Danvers, MA), mouse anti-PKCε (1:1000; Santa Cruz), rabbit anti-pPKCε (1:1000; Santa Cruz), and rabbit anti-P2X3R (1:2000; Alomone Labs). β-Actin, probed with anti-β-actin (1:10,000; Chemicon, Temeculla, CA), or the neuronal marker, β-tubulin, probed with anti β-tubulin antibody (1:2000; Santa Cruz), was used as loading control.

    Techniques: Expressing

    Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats, Go6976 (11.5 pmol = 4.35 ng in 50-μL saline) had no effect on α,β-meATP–elicited flinch activity. In complete Freund adjuvant–treated rats, Go6976 inhibited the enhanced α,β-meATP–induced flinch responses (n = 6; * P

    Journal: Pain

    Article Title: Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation

    doi: 10.1097/j.pain.0000000000000547

    Figure Lengend Snippet: Go6976 reduces α,β-meATP–elicited flinch nocifensive responses in complete Freund adjuvant–treated rats, and PKCβ1 is not expressed in P2X3R-containing dorsal root ganglia (DRG) neurons. (A) In saline control rats, Go6976 (11.5 pmol = 4.35 ng in 50-μL saline) had no effect on α,β-meATP–elicited flinch activity. In complete Freund adjuvant–treated rats, Go6976 inhibited the enhanced α,β-meATP–induced flinch responses (n = 6; * P

    Article Snippet: Antibodies used were mouse anti-Epac1, anti-Epac2 (1:1000; Cell Signaling, Danvers, MA), mouse anti-PKCε (1:1000; Santa Cruz), rabbit anti-pPKCε (1:1000; Santa Cruz), and rabbit anti-P2X3R (1:2000; Alomone Labs). β-Actin, probed with anti-β-actin (1:10,000; Chemicon, Temeculla, CA), or the neuronal marker, β-tubulin, probed with anti β-tubulin antibody (1:2000; Santa Cruz), was used as loading control.

    Techniques: Activity Assay

    Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found both at the cell membrane and in the cytoplasm. P2X3Rs were colocalized with pPKCα in small or medium cells. Enlarged views of cells (indicated by arrows) are shown in the lower left corners. Lower: pPKCε was expressed in both small or medium and large cells, most contained punctate labels. pPKCε labels were colocalized with P2X3Rs in small or medium cells (bar scale = 25 μm).

    Journal: Pain

    Article Title: Epac–protein kinase C alpha signaling in purinergic P2X3R-mediated hyperalgesia after inflammation

    doi: 10.1097/j.pain.0000000000000547

    Figure Lengend Snippet: Phosphorylated PKC (pPKC) isoform and P2X3R expression in dorsal root ganglia slices prepared from complete Freund adjuvant–treated rats. Upper: pPKCα and P2X3Rs were expressed only in small or medium cells. pPKCα labels were found both at the cell membrane and in the cytoplasm. P2X3Rs were colocalized with pPKCα in small or medium cells. Enlarged views of cells (indicated by arrows) are shown in the lower left corners. Lower: pPKCε was expressed in both small or medium and large cells, most contained punctate labels. pPKCε labels were colocalized with P2X3Rs in small or medium cells (bar scale = 25 μm).

    Article Snippet: Antibodies used were mouse anti-Epac1, anti-Epac2 (1:1000; Cell Signaling, Danvers, MA), mouse anti-PKCε (1:1000; Santa Cruz), rabbit anti-pPKCε (1:1000; Santa Cruz), and rabbit anti-P2X3R (1:2000; Alomone Labs). β-Actin, probed with anti-β-actin (1:10,000; Chemicon, Temeculla, CA), or the neuronal marker, β-tubulin, probed with anti β-tubulin antibody (1:2000; Santa Cruz), was used as loading control.

    Techniques: Expressing

    Expression of purinergic receptors and pannexin 1 channels is altered in T1D Akita mice bones. (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P

    Journal: PLoS ONE

    Article Title: P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes

    doi: 10.1371/journal.pone.0155107

    Figure Lengend Snippet: Expression of purinergic receptors and pannexin 1 channels is altered in T1D Akita mice bones. (A) Relative protein expression levels of P2R subtypes (P2Y 1 R, P2Y 2 R, P2Y 4 R, P2X3R, P2X4R and P2X7R) and Panx1 in 8-week-old Akita and age-matched wildtype bone tissue (Data are presented as the means ± SEM *P

    Article Snippet: The membranes were probed with primary polyclonal antibodies to P2Y1 R (1:1000; APR-009), P2Y2 R (1:500; APR-010), P2Y4 R (1:500; APR-006), P2X1R (1:1000; APR-001), P2X3R (1:1000; APR-016), P2X4R (1:1000; APR-002), P2X7R (1:1000; APR-004, Alomone Labs, Israel), Panx1 (N [Term], 1:100; Cat 487900, Invitrogen), GRP78 BiP (1:5000; ab21685, Abcam, Cambridge, MA, USA) and β-actin (1:35000; A1978, Sigma-Aldrich) followed by incubation with respective horseradish peroxidase (HRP)-conjugated anti-rabbit IgG and anti-mouse IgG (1:10000; Santa Cruz Biotechnology, TX, USA).

    Techniques: Expressing, Mouse Assay

    CPT increases the membrane expression of P2X3Rs in cultured DRGs. The P2X3R levels in total protein (Total), membrane protein (Mem), and cytoplasmic protein samples (Cytosol) before (Con) and after CPT (1 µM, 10 min) treatment were determined. Total, membrane, and cytosol protein samples were loaded onto gels. The expression of the ubiquitous membrane protein Na+/K+ ATPase was probed to indicate the membrane expressed protein levels in our samples. The expressions of β-tubulin used as sample loading controls were also indicated. CPT significantly increased P2X3Rs expressed in the cell membrane (total protein: CPT/Con = 1.19; membrane protein: CPT/Con = 1.93 and cytoplasmic protein: CPT/Con = 0.75). N = 3. * P

    Journal: Molecular Pain

    Article Title: F-actin links Epac-PKC signaling to purinergic P2X3 receptor sensitization in dorsal root ganglia following inflammation

    doi: 10.1177/1744806916660557

    Figure Lengend Snippet: CPT increases the membrane expression of P2X3Rs in cultured DRGs. The P2X3R levels in total protein (Total), membrane protein (Mem), and cytoplasmic protein samples (Cytosol) before (Con) and after CPT (1 µM, 10 min) treatment were determined. Total, membrane, and cytosol protein samples were loaded onto gels. The expression of the ubiquitous membrane protein Na+/K+ ATPase was probed to indicate the membrane expressed protein levels in our samples. The expressions of β-tubulin used as sample loading controls were also indicated. CPT significantly increased P2X3Rs expressed in the cell membrane (total protein: CPT/Con = 1.19; membrane protein: CPT/Con = 1.93 and cytoplasmic protein: CPT/Con = 0.75). N = 3. * P

    Article Snippet: Antibodies used were rabbit anti-pPKCɛ (1:1000, Santa Cruz, Dallas, TX) and rabbit anti-P2X3R (1:2000, Alomone Labs).

    Techniques: Cycling Probe Technology, Expressing, Cell Culture

    Epac control of the membrane expression of P2X3Rs in DRG neurons is F-actin mediated. (a) The membrane expression of P2X3Rs was examined in cultured DRG neurons using anti-ext-P2X3R antibody. The enlarged views of neurons (indicated by red arrows) are shown on the right side of each panel. Bars = 25 µm. (b) In the presence of CPT (1 µM), the percentage of cells expressing ext-P2X3R increased by 1.80 fold and the intensity of ext-P2X3R labels increased by 5.57 fold. The CPT-induced increase in membrane expressed P2X3Rs was significantly reduced by pre-incubating cells with the F-actin disrupter, LaA (1 µM, 60 min). A total of 1192 cells obtained from three experiments were used for the analyses. * P

    Journal: Molecular Pain

    Article Title: F-actin links Epac-PKC signaling to purinergic P2X3 receptor sensitization in dorsal root ganglia following inflammation

    doi: 10.1177/1744806916660557

    Figure Lengend Snippet: Epac control of the membrane expression of P2X3Rs in DRG neurons is F-actin mediated. (a) The membrane expression of P2X3Rs was examined in cultured DRG neurons using anti-ext-P2X3R antibody. The enlarged views of neurons (indicated by red arrows) are shown on the right side of each panel. Bars = 25 µm. (b) In the presence of CPT (1 µM), the percentage of cells expressing ext-P2X3R increased by 1.80 fold and the intensity of ext-P2X3R labels increased by 5.57 fold. The CPT-induced increase in membrane expressed P2X3Rs was significantly reduced by pre-incubating cells with the F-actin disrupter, LaA (1 µM, 60 min). A total of 1192 cells obtained from three experiments were used for the analyses. * P

    Article Snippet: Antibodies used were rabbit anti-pPKCɛ (1:1000, Santa Cruz, Dallas, TX) and rabbit anti-P2X3R (1:2000, Alomone Labs).

    Techniques: Expressing, Cell Culture, Cycling Probe Technology