Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Western blot analysis Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot

Journal: BioMed Research International

Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

doi: 10.1155/2020/9861459

Figure Lengend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

Article Snippet: The primary antibodies were rabbit anti-P2X1-6 (RRID/Cat: AB_2341048, #APR-022; AB_2341051, #APR-025; AB_2341052, #APR-026; AB_2341050, #APR-024; AB_2756757, #APR-027; and AB_2756758, #APR-028, respectively, diluted 1 : 200 with 0.1 M PBS, Alomone Labs, Israel).

Techniques: Expressing, Fluorescence