rabbit anti nestin polyclonal antibody  (Boster Bio)


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    Boster Bio rabbit anti nestin polyclonal antibody
    Rabbit Anti Nestin Polyclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nestin polyclonal antibody/product/Boster Bio
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nestin polyclonal antibody - by Bioz Stars, 2022-07
    90/100 stars

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    Boster Bio rabbit anti rat nestin
    <t>Nestin/microtubule-associated</t> protein-2 <t>(MAP2)-positive</t> cells in the unaffected hemisphere in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. MAP2-positive cells are stained red. In the cytoplasm the Nestin/MAP2-positive cells were mainly yellow (arrows). a P
    Rabbit Anti Rat Nestin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat nestin/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat nestin - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

    93
    Boster Bio anti nestin
    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers <t>SOX2,</t> <t>NESTIN,</t> VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.
    Anti Nestin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nestin/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nestin - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    90
    Boster Bio rabbit anti nestin polyclonal antibody
    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers <t>SOX2,</t> <t>NESTIN,</t> VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.
    Rabbit Anti Nestin Polyclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nestin polyclonal antibody/product/Boster Bio
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nestin polyclonal antibody - by Bioz Stars, 2022-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Nestin/microtubule-associated protein-2 (MAP2)-positive cells in the unaffected hemisphere in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. MAP2-positive cells are stained red. In the cytoplasm the Nestin/MAP2-positive cells were mainly yellow (arrows). a P

    Journal: Neural Regeneration Research

    Article Title: Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats ☆

    doi: 10.3969/j.issn.1673-5374.2012.34.007

    Figure Lengend Snippet: Nestin/microtubule-associated protein-2 (MAP2)-positive cells in the unaffected hemisphere in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. MAP2-positive cells are stained red. In the cytoplasm the Nestin/MAP2-positive cells were mainly yellow (arrows). a P

    Article Snippet: Immunofluorescent double staining of nestin/MAP2 and nestin/GFAP Brain slices were incubated with rabbit anti-rat nestin (1:200; Wuhan Boster, China) at 37°C for 2 hours and goat anti-rabbit IgG/Dylight 488 (1:100; Jackson ImmunoResearch Inc., West Grove, PA, USA) at 4°C overnignt, then with rabbit anti-rat GFAP or rabbit anti-rat MAP2 (1:200; Wuhan Boster) at 37°C for 2 hours followed by 4°C overnight.

    Techniques: Staining

    Nestin/glial fibrillary acidic protein (GFAP)-positive cells in the peripheral infarct zone in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. GFAP-positive cells are stained red. In the cytoplasm, the nestin/GFAP-positive cells were mainly yellow. The number of nestin/GFAP-positive cells in the ipsilateral limb-training group and bilateral limb-training group was significantly higher than that in the untreated group at postoperative day 3, and this effect lasted until postoperative day 14 ( a P

    Journal: Neural Regeneration Research

    Article Title: Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats ☆

    doi: 10.3969/j.issn.1673-5374.2012.34.007

    Figure Lengend Snippet: Nestin/glial fibrillary acidic protein (GFAP)-positive cells in the peripheral infarct zone in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. GFAP-positive cells are stained red. In the cytoplasm, the nestin/GFAP-positive cells were mainly yellow. The number of nestin/GFAP-positive cells in the ipsilateral limb-training group and bilateral limb-training group was significantly higher than that in the untreated group at postoperative day 3, and this effect lasted until postoperative day 14 ( a P

    Article Snippet: Immunofluorescent double staining of nestin/MAP2 and nestin/GFAP Brain slices were incubated with rabbit anti-rat nestin (1:200; Wuhan Boster, China) at 37°C for 2 hours and goat anti-rabbit IgG/Dylight 488 (1:100; Jackson ImmunoResearch Inc., West Grove, PA, USA) at 4°C overnignt, then with rabbit anti-rat GFAP or rabbit anti-rat MAP2 (1:200; Wuhan Boster) at 37°C for 2 hours followed by 4°C overnight.

    Techniques: Staining

    Nestin/microtubule-associated protein-2 (MAP2)-positive cells in the peripheral infarct zone in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. MAP2-positive cells are stained red. In the cytoplasm, the nestin/MAP2-positive cells were mainly yellow. a P

    Journal: Neural Regeneration Research

    Article Title: Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats ☆

    doi: 10.3969/j.issn.1673-5374.2012.34.007

    Figure Lengend Snippet: Nestin/microtubule-associated protein-2 (MAP2)-positive cells in the peripheral infarct zone in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. MAP2-positive cells are stained red. In the cytoplasm, the nestin/MAP2-positive cells were mainly yellow. a P

    Article Snippet: Immunofluorescent double staining of nestin/MAP2 and nestin/GFAP Brain slices were incubated with rabbit anti-rat nestin (1:200; Wuhan Boster, China) at 37°C for 2 hours and goat anti-rabbit IgG/Dylight 488 (1:100; Jackson ImmunoResearch Inc., West Grove, PA, USA) at 4°C overnignt, then with rabbit anti-rat GFAP or rabbit anti-rat MAP2 (1:200; Wuhan Boster) at 37°C for 2 hours followed by 4°C overnight.

    Techniques: Staining

    Nestin/glial fibrillary acidic protein (GFAP) in the unaffected hemisphere in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. GFAP-positive cells are stained red. In the cytoplasm the nestin/GFAP-positive cells were mainly yellow (arrows). a P

    Journal: Neural Regeneration Research

    Article Title: Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats ☆

    doi: 10.3969/j.issn.1673-5374.2012.34.007

    Figure Lengend Snippet: Nestin/glial fibrillary acidic protein (GFAP) in the unaffected hemisphere in middle cerebral artery occlusion rats following ipsilateral or bilateral limb-training. In the gross specimen, the white area represents the cerebral infarction area. Nestin-positive cells are stained green. GFAP-positive cells are stained red. In the cytoplasm the nestin/GFAP-positive cells were mainly yellow (arrows). a P

    Article Snippet: Immunofluorescent double staining of nestin/MAP2 and nestin/GFAP Brain slices were incubated with rabbit anti-rat nestin (1:200; Wuhan Boster, China) at 37°C for 2 hours and goat anti-rabbit IgG/Dylight 488 (1:100; Jackson ImmunoResearch Inc., West Grove, PA, USA) at 4°C overnignt, then with rabbit anti-rat GFAP or rabbit anti-rat MAP2 (1:200; Wuhan Boster) at 37°C for 2 hours followed by 4°C overnight.

    Techniques: Staining

    Overexpression of GAP-43 promotes the differentiation of BMSCs into neuron-like cells. (A) Western blot analysis demonstrated that the protein expression of the GAP-43 was significantly increased in the LV5-GAP-43 group, compared with the NC and Blank groups. (B) BMSCs were observed using microscopy and it was revealed that BMSCs exhibited a neuronal phenotype following transduction with LV5-GAP-43. (C) BMSCs expressed positive specific neural markers for NSE, NF, nestin and βIII-tubulin in the LV5-GAP-43 group, determined using semi-quantitative polymerase chain reaction detection. Scale bar=50 µ m. GAP-43, growth-associated protein-43; BMSCs, bone marrow mesenchymal stem cells; NSE, neuron-specific enolase; NF, neurofilament; NC, transduced with negative control scramble lentiviral vector; LV5-GAP-43, transduced with LV5-GAP-43; M, marker.

    Journal: Molecular Medicine Reports

    Article Title: Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats

    doi: 10.3892/mmr.2015.4132

    Figure Lengend Snippet: Overexpression of GAP-43 promotes the differentiation of BMSCs into neuron-like cells. (A) Western blot analysis demonstrated that the protein expression of the GAP-43 was significantly increased in the LV5-GAP-43 group, compared with the NC and Blank groups. (B) BMSCs were observed using microscopy and it was revealed that BMSCs exhibited a neuronal phenotype following transduction with LV5-GAP-43. (C) BMSCs expressed positive specific neural markers for NSE, NF, nestin and βIII-tubulin in the LV5-GAP-43 group, determined using semi-quantitative polymerase chain reaction detection. Scale bar=50 µ m. GAP-43, growth-associated protein-43; BMSCs, bone marrow mesenchymal stem cells; NSE, neuron-specific enolase; NF, neurofilament; NC, transduced with negative control scramble lentiviral vector; LV5-GAP-43, transduced with LV5-GAP-43; M, marker.

    Article Snippet: The samples were then incubated with rabbit anti-rat nestin, NeuN and GAP-43 polyclonal antibodies (Wuhan Boster Biological Technology, Ltd.) at a dilution of 1:100 in PBS at 4°C overnight, washed twice with PBS and incubated with FITC- or Cy3-labeled rabbit anti-goat IgG (H+L; Wuhan Boster Biological Technology, Ltd.) at a dilution of 1:200 in PBS at room temperature for 1 h. Finally, images were captured using an inverted fluorescence microscope (Olympus Corp.).

    Techniques: Over Expression, Western Blot, Expressing, Microscopy, Transduction, Real-time Polymerase Chain Reaction, Negative Control, Plasmid Preparation, Marker

    Ability of BMSCs to differentiate into neuron-like cells is significantly weakened when the expression of GAP-43 in BMSCs is inhibited. (A) Western blot analysis demonstrated that LV3-shRNA-GAP-43-619 was the most effective siRNA against GAP-43. 1, transduced with LV5-GAP-43; 2, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-844; 3, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-1215; 4, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-678; 5, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-619. (B) Western blot analysis revealed that the protein expression of the GAP-43 was significantly decreased in the LV3-shRNA-GAP-43-619 group, compared with the NC and Blank groups. (C) BMSCs exhibited a neuronal phenotype in the NC and Blank groups; however, the majority of BMSCs exhibited no marked morphological changes. (D) Western blot analysis demonstrated that the expression levels of NSE, NF, nestin and βIII-tubulin were decreased in the LV3-shRNA-GAP-43-619 group compared with NC and Blank groups. (E) Cell immunofluorescence analysis revealed that the expression of GAP-43, nestin and NeuN were decreased in the LV3-shRNA-GAP-43-619 group, compared with the NC group under induction conditions. Scale bar=50 µ m. * P

    Journal: Molecular Medicine Reports

    Article Title: Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats

    doi: 10.3892/mmr.2015.4132

    Figure Lengend Snippet: Ability of BMSCs to differentiate into neuron-like cells is significantly weakened when the expression of GAP-43 in BMSCs is inhibited. (A) Western blot analysis demonstrated that LV3-shRNA-GAP-43-619 was the most effective siRNA against GAP-43. 1, transduced with LV5-GAP-43; 2, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-844; 3, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-1215; 4, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-678; 5, transduced with LV5-GAP-43 and LV3-shRNA-GAP-43-619. (B) Western blot analysis revealed that the protein expression of the GAP-43 was significantly decreased in the LV3-shRNA-GAP-43-619 group, compared with the NC and Blank groups. (C) BMSCs exhibited a neuronal phenotype in the NC and Blank groups; however, the majority of BMSCs exhibited no marked morphological changes. (D) Western blot analysis demonstrated that the expression levels of NSE, NF, nestin and βIII-tubulin were decreased in the LV3-shRNA-GAP-43-619 group compared with NC and Blank groups. (E) Cell immunofluorescence analysis revealed that the expression of GAP-43, nestin and NeuN were decreased in the LV3-shRNA-GAP-43-619 group, compared with the NC group under induction conditions. Scale bar=50 µ m. * P

    Article Snippet: The samples were then incubated with rabbit anti-rat nestin, NeuN and GAP-43 polyclonal antibodies (Wuhan Boster Biological Technology, Ltd.) at a dilution of 1:100 in PBS at 4°C overnight, washed twice with PBS and incubated with FITC- or Cy3-labeled rabbit anti-goat IgG (H+L; Wuhan Boster Biological Technology, Ltd.) at a dilution of 1:200 in PBS at room temperature for 1 h. Finally, images were captured using an inverted fluorescence microscope (Olympus Corp.).

    Techniques: Expressing, Western Blot, shRNA, Transduction, Immunofluorescence

    Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.

    Journal: American Journal of Translational Research

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    doi:

    Figure Lengend Snippet: Rough distribution of the stem cells in the primary and recurrent pterygium tissue. A. H E staining of the primary and recurrent pterygium tissue. B. Immunohistochemistry staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the primary pterygium tissues. C. Immunohistochemically staining for stem cell markers SOX2, NESTIN, VIMENTIN and CD44 in the recurrent pterygium tissues. The scale bars are 100 μm.

    Article Snippet: For immunohistochemistry staining of stem cells located in pterygium tissues, the sections were incubated with primary antibodies anti-SOX2 (rabbit monoclonal: dilution 1:300: abcam), anti-NESTIN (rabbit polyclonal; dilution 1:100: boster), anti-VIMENTIN (mouse polyclonal; dilution 1:100: boster), and anti-CD44 (rabbit polyclonal; dilution 1:100: boster) at 4°C overnight, followed by exposure to 3% H2 O2 and blocked by 5% BSA.

    Techniques: Staining, Immunohistochemistry

    Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Journal: American Journal of Translational Research

    Article Title: Identification and differentiation therapy strategy of pterygium in vitro

    doi:

    Figure Lengend Snippet: Pterygium stem cells induced spheres retain characteristic of stem cells. The spheres expressed multi-lineage stem cells markers including SOX2, SOX9, SOX10, NESTIN, VIMENTIN and CD44. IgG-Cy3 (red) was used as the secondary antibody. The nuclei were counterstained with Hoechst 33342 (blue). The scale bars are 50 μm.

    Article Snippet: For immunohistochemistry staining of stem cells located in pterygium tissues, the sections were incubated with primary antibodies anti-SOX2 (rabbit monoclonal: dilution 1:300: abcam), anti-NESTIN (rabbit polyclonal; dilution 1:100: boster), anti-VIMENTIN (mouse polyclonal; dilution 1:100: boster), and anti-CD44 (rabbit polyclonal; dilution 1:100: boster) at 4°C overnight, followed by exposure to 3% H2 O2 and blocked by 5% BSA.

    Techniques: