rabbit anti nav1  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti nav1
    Unaltered internodal length and myelin sheath thickness in Pten fl/+ Dhh cre/+ mice. a G-ratio plotted against axon diameter of wildtype (WT, grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at P18. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ and Pmp22 tg mice compared to WT controls and decreased in Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 3, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 5 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 3 animals. b G-ratio plotted against axon diameter of WT (grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at 16 weeks of age. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ mice compared to WT controls and decreased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 4, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 3 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 4 animals. c The number of slips on the elevated beam is similarly increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls at all time points. Behavioral analysis was done at 12, 16 and 24 weeks of age. WT n = 10-14, Pten fl/+ Dhh cre/+ n = 6-14, Pmp22 tg n = 9-19 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 9-14 mice were analyzed. d Neither the weight of Pmp22 tg nor Pten fl/+ Dhh cre/+ Pmp22 tg mice is altered compared to wildtype controls at 12, 16 and 24 weeks of age. e Sensory nerve action potential amplitudes (SNAP) are decreased in the tail of Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls. For electrophysiology measurements WT n = 10, Pten fl/+ Dhh cre/+ n = 8, Pmp22 tg n = 11 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 8 mice were analyzed. f Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), <t>NaV1.6</t> (magenta) and DAPI (blue) at P18. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) is significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at P18, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Mean fiber diameters are not significantly altered (right panel). Analysis was performed on 100 internodes of n = 3-4 animals per group. g . Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at 16 weeks of age. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) and fiber diameter (right panel) are significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at 16 weeks of age, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Analysis was performed on 100 internodes of n = 3-4 animals per group. Means are displayed ± standard deviation. Statistical analysis was done using one-way ANOVA with Sidak’s multiple comparison test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).
    Rabbit Anti Nav1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nav1/product/Alomone Labs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nav1 - by Bioz Stars, 2022-09
    95/100 stars

    Images

    1) Product Images from "Inversely proportional myelin growth due to altered Pmp22 gene dosage identifies PI3K/Akt/mTOR signaling as a novel therapeutic target in HNPP"

    Article Title: Inversely proportional myelin growth due to altered Pmp22 gene dosage identifies PI3K/Akt/mTOR signaling as a novel therapeutic target in HNPP

    Journal: bioRxiv

    doi: 10.1101/2021.11.08.467756

    Unaltered internodal length and myelin sheath thickness in Pten fl/+ Dhh cre/+ mice. a G-ratio plotted against axon diameter of wildtype (WT, grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at P18. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ and Pmp22 tg mice compared to WT controls and decreased in Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 3, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 5 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 3 animals. b G-ratio plotted against axon diameter of WT (grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at 16 weeks of age. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ mice compared to WT controls and decreased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 4, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 3 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 4 animals. c The number of slips on the elevated beam is similarly increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls at all time points. Behavioral analysis was done at 12, 16 and 24 weeks of age. WT n = 10-14, Pten fl/+ Dhh cre/+ n = 6-14, Pmp22 tg n = 9-19 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 9-14 mice were analyzed. d Neither the weight of Pmp22 tg nor Pten fl/+ Dhh cre/+ Pmp22 tg mice is altered compared to wildtype controls at 12, 16 and 24 weeks of age. e Sensory nerve action potential amplitudes (SNAP) are decreased in the tail of Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls. For electrophysiology measurements WT n = 10, Pten fl/+ Dhh cre/+ n = 8, Pmp22 tg n = 11 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 8 mice were analyzed. f Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at P18. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) is significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at P18, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Mean fiber diameters are not significantly altered (right panel). Analysis was performed on 100 internodes of n = 3-4 animals per group. g . Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at 16 weeks of age. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) and fiber diameter (right panel) are significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at 16 weeks of age, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Analysis was performed on 100 internodes of n = 3-4 animals per group. Means are displayed ± standard deviation. Statistical analysis was done using one-way ANOVA with Sidak’s multiple comparison test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).
    Figure Legend Snippet: Unaltered internodal length and myelin sheath thickness in Pten fl/+ Dhh cre/+ mice. a G-ratio plotted against axon diameter of wildtype (WT, grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at P18. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ and Pmp22 tg mice compared to WT controls and decreased in Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 3, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 5 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 3 animals. b G-ratio plotted against axon diameter of WT (grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at 16 weeks of age. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ mice compared to WT controls and decreased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 4, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 3 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 4 animals. c The number of slips on the elevated beam is similarly increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls at all time points. Behavioral analysis was done at 12, 16 and 24 weeks of age. WT n = 10-14, Pten fl/+ Dhh cre/+ n = 6-14, Pmp22 tg n = 9-19 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 9-14 mice were analyzed. d Neither the weight of Pmp22 tg nor Pten fl/+ Dhh cre/+ Pmp22 tg mice is altered compared to wildtype controls at 12, 16 and 24 weeks of age. e Sensory nerve action potential amplitudes (SNAP) are decreased in the tail of Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls. For electrophysiology measurements WT n = 10, Pten fl/+ Dhh cre/+ n = 8, Pmp22 tg n = 11 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 8 mice were analyzed. f Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at P18. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) is significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at P18, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Mean fiber diameters are not significantly altered (right panel). Analysis was performed on 100 internodes of n = 3-4 animals per group. g . Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at 16 weeks of age. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) and fiber diameter (right panel) are significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at 16 weeks of age, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Analysis was performed on 100 internodes of n = 3-4 animals per group. Means are displayed ± standard deviation. Statistical analysis was done using one-way ANOVA with Sidak’s multiple comparison test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

    Techniques Used: Mouse Assay, Staining, Standard Deviation

    2) Product Images from "TRPV1 Tunes Optic Nerve Axon Excitability in Glaucoma"

    Article Title: TRPV1 Tunes Optic Nerve Axon Excitability in Glaucoma

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00249

    (A–D) Representative confocal micrographs of Caspr1 (green) and NaV1.6 (red) immunostaining of longitudinal optic nerve sections from wild-type (WT) (A,B) and Trpv1 –/– (C,D) mice. Scale bar = 10 μm.
    Figure Legend Snippet: (A–D) Representative confocal micrographs of Caspr1 (green) and NaV1.6 (red) immunostaining of longitudinal optic nerve sections from wild-type (WT) (A,B) and Trpv1 –/– (C,D) mice. Scale bar = 10 μm.

    Techniques Used: Immunostaining, Mouse Assay

    (A) High magnification confocal micrographs of longitudinal sections through Ctrl and 2 Wk wild-type (WT) and Trpv1 –/– optic nerves show Caspr1-labeled paranodes (green) flanking NaV1.6 (red) within nodes of Ranvier. (B) Trpv1 –/– 2 Wk paranodes contain increased Caspr1 than did WT 2 Wk nerves (left, * p = 0.012) and are shorter (right, * p
    Figure Legend Snippet: (A) High magnification confocal micrographs of longitudinal sections through Ctrl and 2 Wk wild-type (WT) and Trpv1 –/– optic nerves show Caspr1-labeled paranodes (green) flanking NaV1.6 (red) within nodes of Ranvier. (B) Trpv1 –/– 2 Wk paranodes contain increased Caspr1 than did WT 2 Wk nerves (left, * p = 0.012) and are shorter (right, * p

    Techniques Used: Labeling

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    Alomone Labs anti nav1 6 scn8a antibody
    Both I NaP and hyperexcitability induced by PR 20 are blocked by antibodies targeting Nav1.2, β1, and β4. ( A ) A percentage increase graph for changes in I NaP at −20 mV of cortical motor neurons by PR 20 treatment (100 nM, 20 min) in the presence of the control IgG or Nav antibodies as indicated. I NaP was evoked by a depolarizing voltage ramp (−60 to 0 mV, 60 mV/s). All data are mean ± SEM. IgG: n = 5 and 2 mice. Nav1.2: n = 7 and 4 mice. <t>Nav1.6:</t> n = 5 and 2 mice. β1: n = 5 and 2 mice. β2: n = 3 and 2 mice. β4: n = 6 and 3 mice. Numbers of cells/mice analyzed are shown in parentheses. NS indicates not significantly different. * P
    Anti Nav1 6 Scn8a Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nav1 6 scn8a antibody/product/Alomone Labs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti nav1 6 scn8a antibody - by Bioz Stars, 2022-09
    95/100 stars
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    Both I NaP and hyperexcitability induced by PR 20 are blocked by antibodies targeting Nav1.2, β1, and β4. ( A ) A percentage increase graph for changes in I NaP at −20 mV of cortical motor neurons by PR 20 treatment (100 nM, 20 min) in the presence of the control IgG or Nav antibodies as indicated. I NaP was evoked by a depolarizing voltage ramp (−60 to 0 mV, 60 mV/s). All data are mean ± SEM. IgG: n = 5 and 2 mice. Nav1.2: n = 7 and 4 mice. Nav1.6: n = 5 and 2 mice. β1: n = 5 and 2 mice. β2: n = 3 and 2 mice. β4: n = 6 and 3 mice. Numbers of cells/mice analyzed are shown in parentheses. NS indicates not significantly different. * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Poly-dipeptides produced from C9orf72 hexanucleotide repeats cause selective motor neuron hyperexcitability in ALS

    doi: 10.1073/pnas.2113813119

    Figure Lengend Snippet: Both I NaP and hyperexcitability induced by PR 20 are blocked by antibodies targeting Nav1.2, β1, and β4. ( A ) A percentage increase graph for changes in I NaP at −20 mV of cortical motor neurons by PR 20 treatment (100 nM, 20 min) in the presence of the control IgG or Nav antibodies as indicated. I NaP was evoked by a depolarizing voltage ramp (−60 to 0 mV, 60 mV/s). All data are mean ± SEM. IgG: n = 5 and 2 mice. Nav1.2: n = 7 and 4 mice. Nav1.6: n = 5 and 2 mice. β1: n = 5 and 2 mice. β2: n = 3 and 2 mice. β4: n = 6 and 3 mice. Numbers of cells/mice analyzed are shown in parentheses. NS indicates not significantly different. * P

    Article Snippet: Antibodies used and sources were as follows: anti-Nav1.2 antibody (ASC-002; Alomone); anti-Nav1.6 antibody (ASC-009; Alomone); anti-Nav β1-antibody (13950; Cell Signaling Technology); anti-Nav β2-antibody (ASC-007; Alomone); anti-Nav β4-antibody (Ab80539; Abcam); anti–β-Actin antibody (A5441; Sigma-Aldrich); anti–β-Tubulin antibody (2146; Cell Signaling Technology); HA (SC-7392; Santa Cruz); and HA magnetic beads (88837; Thermo Fisher).

    Techniques: Mouse Assay

    Unaltered internodal length and myelin sheath thickness in Pten fl/+ Dhh cre/+ mice. a G-ratio plotted against axon diameter of wildtype (WT, grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at P18. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ and Pmp22 tg mice compared to WT controls and decreased in Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 3, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 5 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 3 animals. b G-ratio plotted against axon diameter of WT (grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at 16 weeks of age. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ mice compared to WT controls and decreased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 4, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 3 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 4 animals. c The number of slips on the elevated beam is similarly increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls at all time points. Behavioral analysis was done at 12, 16 and 24 weeks of age. WT n = 10-14, Pten fl/+ Dhh cre/+ n = 6-14, Pmp22 tg n = 9-19 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 9-14 mice were analyzed. d Neither the weight of Pmp22 tg nor Pten fl/+ Dhh cre/+ Pmp22 tg mice is altered compared to wildtype controls at 12, 16 and 24 weeks of age. e Sensory nerve action potential amplitudes (SNAP) are decreased in the tail of Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls. For electrophysiology measurements WT n = 10, Pten fl/+ Dhh cre/+ n = 8, Pmp22 tg n = 11 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 8 mice were analyzed. f Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at P18. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) is significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at P18, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Mean fiber diameters are not significantly altered (right panel). Analysis was performed on 100 internodes of n = 3-4 animals per group. g . Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at 16 weeks of age. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) and fiber diameter (right panel) are significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at 16 weeks of age, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Analysis was performed on 100 internodes of n = 3-4 animals per group. Means are displayed ± standard deviation. Statistical analysis was done using one-way ANOVA with Sidak’s multiple comparison test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

    Journal: bioRxiv

    Article Title: Inversely proportional myelin growth due to altered Pmp22 gene dosage identifies PI3K/Akt/mTOR signaling as a novel therapeutic target in HNPP

    doi: 10.1101/2021.11.08.467756

    Figure Lengend Snippet: Unaltered internodal length and myelin sheath thickness in Pten fl/+ Dhh cre/+ mice. a G-ratio plotted against axon diameter of wildtype (WT, grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at P18. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ and Pmp22 tg mice compared to WT controls and decreased in Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 3, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 5 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 3 animals. b G-ratio plotted against axon diameter of WT (grey) and Pten fl/+ Dhh cre/+ (turquoise) femoral nerves at 16 weeks of age. Mean g-ratio is unaltered in Pten fl/+ Dhh cre/+ mice compared to WT controls and decreased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (left panel). Mean axon diameters are reduced in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice (middle panel). The number of Schwann cell nuclei per femoral nerve cross section is increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice. WT n = 4, Pten fl/+ Dhh cre/+ n = 4, Pmp22 tg n = 3 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 4 animals. c The number of slips on the elevated beam is similarly increased in Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls at all time points. Behavioral analysis was done at 12, 16 and 24 weeks of age. WT n = 10-14, Pten fl/+ Dhh cre/+ n = 6-14, Pmp22 tg n = 9-19 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 9-14 mice were analyzed. d Neither the weight of Pmp22 tg nor Pten fl/+ Dhh cre/+ Pmp22 tg mice is altered compared to wildtype controls at 12, 16 and 24 weeks of age. e Sensory nerve action potential amplitudes (SNAP) are decreased in the tail of Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg mice compared to wildtype controls. For electrophysiology measurements WT n = 10, Pten fl/+ Dhh cre/+ n = 8, Pmp22 tg n = 11 and Pten fl/+ Dhh cre/+ Pmp22 tg n = 8 mice were analyzed. f Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at P18. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) is significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at P18, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Mean fiber diameters are not significantly altered (right panel). Analysis was performed on 100 internodes of n = 3-4 animals per group. g . Example images of teased fiber preparations of WT, Pten fl/+ Dhh cre/+ , Pmp22 tg and Pten fl/+ Dhh cre/+ Pmp22 tg double mutants stained for MAG (green), NaV1.6 (magenta) and DAPI (blue) at 16 weeks of age. Internodes between two nodes (magenta arrowheads) are underlined in yellow and respective Schwann cell nuclei are marked by white stars. Mean internodal length (left panel) and fiber diameter (right panel) are significantly reduced in Pmp22 tg teased fibers compared to wildtype controls at 16 weeks of age, whereas Pten fl/+ Dhh cre/+ Pmp22 tg mice do not differ in internodal length compared to Pmp22 tg mice. Analysis was performed on 100 internodes of n = 3-4 animals per group. Means are displayed ± standard deviation. Statistical analysis was done using one-way ANOVA with Sidak’s multiple comparison test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

    Article Snippet: After incubation with primary antibodies (mouse anti MAG 1:50 (Chemicon clone 513), rabbit anti NaV1.6 1:250 (Alomone Labs #ASC-009)) in blocking solution, three washing steps in PBS followed.

    Techniques: Mouse Assay, Staining, Standard Deviation

    Myelin internode length following partial ON transection. Representative images of a single slice from the z stacks show ventral axons of control animals (A) and at 3 months following injury (B) anterogradely traced with CTB (green); paranodes are immunohistochemically labelled with Caspr and nodes with Nav1.6. C: The length of myelin internodes (indicated by

    Journal: PLoS ONE

    Article Title: Early Proliferation Does Not Prevent the Loss of Oligodendrocyte Progenitor Cells during the Chronic Phase of Secondary Degeneration in a CNS White Matter Tract

    doi: 10.1371/journal.pone.0065710

    Figure Lengend Snippet: Myelin internode length following partial ON transection. Representative images of a single slice from the z stacks show ventral axons of control animals (A) and at 3 months following injury (B) anterogradely traced with CTB (green); paranodes are immunohistochemically labelled with Caspr and nodes with Nav1.6. C: The length of myelin internodes (indicated by

    Article Snippet: Animals were transcardially perfused 3 days following CTB injection, ONs were post-fixed and longitudinal sections (14 µm) incubated overnight at 4°C with primary antibodies mouse anti-Caspr (1∶750, Abcam) and rabbit anti-Nav1.6 (1∶125, Alamone Laboratories) secondary antibody AlexaFluor® 546 or 647 and sections mounted with ProLong® Gold.

    Techniques: CtB Assay

    (A–D) Representative confocal micrographs of Caspr1 (green) and NaV1.6 (red) immunostaining of longitudinal optic nerve sections from wild-type (WT) (A,B) and Trpv1 –/– (C,D) mice. Scale bar = 10 μm.

    Journal: Frontiers in Physiology

    Article Title: TRPV1 Tunes Optic Nerve Axon Excitability in Glaucoma

    doi: 10.3389/fphys.2020.00249

    Figure Lengend Snippet: (A–D) Representative confocal micrographs of Caspr1 (green) and NaV1.6 (red) immunostaining of longitudinal optic nerve sections from wild-type (WT) (A,B) and Trpv1 –/– (C,D) mice. Scale bar = 10 μm.

    Article Snippet: Primary antibodies used for optic nerve sections were mouse-contactin-associated protein 1 (Caspr1, 1:300, Millipore) and rabbit-NaV1.6 (1:200, Alomone).

    Techniques: Immunostaining, Mouse Assay

    (A) High magnification confocal micrographs of longitudinal sections through Ctrl and 2 Wk wild-type (WT) and Trpv1 –/– optic nerves show Caspr1-labeled paranodes (green) flanking NaV1.6 (red) within nodes of Ranvier. (B) Trpv1 –/– 2 Wk paranodes contain increased Caspr1 than did WT 2 Wk nerves (left, * p = 0.012) and are shorter (right, * p

    Journal: Frontiers in Physiology

    Article Title: TRPV1 Tunes Optic Nerve Axon Excitability in Glaucoma

    doi: 10.3389/fphys.2020.00249

    Figure Lengend Snippet: (A) High magnification confocal micrographs of longitudinal sections through Ctrl and 2 Wk wild-type (WT) and Trpv1 –/– optic nerves show Caspr1-labeled paranodes (green) flanking NaV1.6 (red) within nodes of Ranvier. (B) Trpv1 –/– 2 Wk paranodes contain increased Caspr1 than did WT 2 Wk nerves (left, * p = 0.012) and are shorter (right, * p

    Article Snippet: Primary antibodies used for optic nerve sections were mouse-contactin-associated protein 1 (Caspr1, 1:300, Millipore) and rabbit-NaV1.6 (1:200, Alomone).

    Techniques: Labeling