Article Title: SNTA1 Gene Rescues Ion Channel Function in Cardiomyocytes Derived from Induced Pluripotent Stem Cells Reprogrammed from Muscular Dystrophy Patients with Arrhythmias
Figure Lengend Snippet: NaV1.5 protein level is significantly reduced in patient-specific DMD iPSC-CMs. (a) Representative Western blot for each antibody used. The bands within the blue rectangles at ∼250 KDa and below 50 KDa correspond to NaV1.5 and Kir2.1, respectively. About 50K cells were collected to quantify total NaV1.5, Kir2.1 and actinin levels in control, heterozygous and hemizygous DMD cells. (b) Scatter plots of NaV1.5 and Kir2.1 detected in control, female and DMD iPSC-CMs. NaV1.5 and Kir2.1 protein levels were normalized to actinin (loading control). (c) Representative Western blot after biotinylation and protein precipitation with streptavidin magnetic beads. (d) Scatter plots of biotinylated NaV1.5 and Kir2.1 from control, female , and DMD iPSC-CMs. Fifty μg of biotinylated protein was loaded. Errors bars represent SEM. The n -values are in parentheses. Two-tailed Mann-Whitney test. *** P
Article Snippet: Rabbit anti-NaV 1.5 antibody (clone ASC-013, Alomone Labs) was used for NaV1.5 protein expression (1:500), mouse anti-Kir2.1 antibody (clone N112B/14, University of California at Davis/Nacional Institutes of Health 105 NeuroMab Facility) was used for Kir2.1 protein expression (1:500), mouse anti-Dystrophin (1:000, #D8043, Sigma) was used to detect the Dp427 dystrophin isoform.
Techniques: Western Blot, Magnetic Beads, Two Tailed Test, MANN-WHITNEY