rabbit anti nav 1 5 antibodies  (Alomone Labs)


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    Alomone Labs rabbit anti nav 1 5 antibodies
    Rabbit Anti Nav 1 5 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nav 1 5 antibodies/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nav 1 5 antibodies - by Bioz Stars, 2022-07
    93/100 stars

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    Alomone Labs rabbit polyclonal anti nav 1 5 antibody
    Identification of native NaV 1.5 phosphorylation sites from WT and CaMKIIδc -Tg mouse ventricles
    Rabbit Polyclonal Anti Nav 1 5 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nav 1 5 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti nav 1 5 antibody - by Bioz Stars, 2022-07
    95/100 stars
      Buy from Supplier

    94
    Alomone Labs anti nav1 5 scn5a 1978 2016 antibody
    <t>NaV1.5</t> protein level is significantly reduced in patient-specific DMD iPSC-CMs. (a) Representative Western blot for each antibody used. The bands within the blue rectangles at ∼250 KDa and below 50 KDa correspond to <t>NaV1.5</t> and Kir2.1, respectively. About 50K cells were collected to quantify total NaV1.5, Kir2.1 and actinin levels in control, heterozygous and hemizygous DMD cells. (b) Scatter plots of NaV1.5 and Kir2.1 detected in control, female and DMD iPSC-CMs. NaV1.5 and Kir2.1 protein levels were normalized to actinin (loading control). (c) Representative Western blot after biotinylation and protein precipitation with streptavidin magnetic beads. (d) Scatter plots of biotinylated NaV1.5 and Kir2.1 from control, female , and DMD iPSC-CMs. Fifty μg of biotinylated protein was loaded. Errors bars represent SEM. The n -values are in parentheses. Two-tailed Mann-Whitney test. *** P
    Anti Nav1 5 Scn5a 1978 2016 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nav1 5 scn5a 1978 2016 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nav1 5 scn5a 1978 2016 antibody - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

    93
    Alomone Labs rabbit anti nav 1 9
    Pruritogens influence NaV 1.9 currents.
    Rabbit Anti Nav 1 9, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nav 1 9/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nav 1 9 - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Identification of native NaV 1.5 phosphorylation sites from WT and CaMKIIδc -Tg mouse ventricles

    Journal: The Journal of Biological Chemistry

    Article Title: C-terminal phosphorylation of NaV1.5 impairs FGF13-dependent regulation of channel inactivation

    doi: 10.1074/jbc.M117.787788

    Figure Lengend Snippet: Identification of native NaV 1.5 phosphorylation sites from WT and CaMKIIδc -Tg mouse ventricles

    Article Snippet: The total ventricular lysates were blotted with a rabbit polyclonal anti-NaV 1.5 antibody (1:2000, RbαNaV 1.5, Alomone, ASC-005).

    Techniques:

    Phosphorylation at serines 1933 and 1984 impairs FGF13-dependent regulation of NaV 1.5 channel inactivation

    Journal: The Journal of Biological Chemistry

    Article Title: C-terminal phosphorylation of NaV1.5 impairs FGF13-dependent regulation of channel inactivation

    doi: 10.1074/jbc.M117.787788

    Figure Lengend Snippet: Phosphorylation at serines 1933 and 1984 impairs FGF13-dependent regulation of NaV 1.5 channel inactivation

    Article Snippet: The total ventricular lysates were blotted with a rabbit polyclonal anti-NaV 1.5 antibody (1:2000, RbαNaV 1.5, Alomone, ASC-005).

    Techniques:

    Phosphorylation at serines 1933 and 1984 impairs FGF13-dependent regulation of NaV 1.5 channel inactivation

    Journal: The Journal of Biological Chemistry

    Article Title: C-terminal phosphorylation of NaV1.5 impairs FGF13-dependent regulation of channel inactivation

    doi: 10.1074/jbc.M117.787788

    Figure Lengend Snippet: Phosphorylation at serines 1933 and 1984 impairs FGF13-dependent regulation of NaV 1.5 channel inactivation

    Article Snippet: The total ventricular lysates were blotted with a rabbit polyclonal anti-NaV 1.5 antibody (1:2000, RbαNaV 1.5, Alomone, ASC-005).

    Techniques:

    NaV1.5 protein level is significantly reduced in patient-specific DMD iPSC-CMs. (a) Representative Western blot for each antibody used. The bands within the blue rectangles at ∼250 KDa and below 50 KDa correspond to NaV1.5 and Kir2.1, respectively. About 50K cells were collected to quantify total NaV1.5, Kir2.1 and actinin levels in control, heterozygous and hemizygous DMD cells. (b) Scatter plots of NaV1.5 and Kir2.1 detected in control, female and DMD iPSC-CMs. NaV1.5 and Kir2.1 protein levels were normalized to actinin (loading control). (c) Representative Western blot after biotinylation and protein precipitation with streptavidin magnetic beads. (d) Scatter plots of biotinylated NaV1.5 and Kir2.1 from control, female , and DMD iPSC-CMs. Fifty μg of biotinylated protein was loaded. Errors bars represent SEM. The n -values are in parentheses. Two-tailed Mann-Whitney test. *** P

    Journal: bioRxiv

    Article Title: SNTA1 Gene Rescues Ion Channel Function in Cardiomyocytes Derived from Induced Pluripotent Stem Cells Reprogrammed from Muscular Dystrophy Patients with Arrhythmias

    doi: 10.1101/2022.01.25.477696

    Figure Lengend Snippet: NaV1.5 protein level is significantly reduced in patient-specific DMD iPSC-CMs. (a) Representative Western blot for each antibody used. The bands within the blue rectangles at ∼250 KDa and below 50 KDa correspond to NaV1.5 and Kir2.1, respectively. About 50K cells were collected to quantify total NaV1.5, Kir2.1 and actinin levels in control, heterozygous and hemizygous DMD cells. (b) Scatter plots of NaV1.5 and Kir2.1 detected in control, female and DMD iPSC-CMs. NaV1.5 and Kir2.1 protein levels were normalized to actinin (loading control). (c) Representative Western blot after biotinylation and protein precipitation with streptavidin magnetic beads. (d) Scatter plots of biotinylated NaV1.5 and Kir2.1 from control, female , and DMD iPSC-CMs. Fifty μg of biotinylated protein was loaded. Errors bars represent SEM. The n -values are in parentheses. Two-tailed Mann-Whitney test. *** P

    Article Snippet: Rabbit anti-NaV 1.5 antibody (clone ASC-013, Alomone Labs) was used for NaV1.5 protein expression (1:500), mouse anti-Kir2.1 antibody (clone N112B/14, University of California at Davis/Nacional Institutes of Health 105 NeuroMab Facility) was used for Kir2.1 protein expression (1:500), mouse anti-Dystrophin (1:000, #D8043, Sigma) was used to detect the Dp427 dystrophin isoform.

    Techniques: Western Blot, Magnetic Beads, Two Tailed Test, MANN-WHITNEY

    Electrophysiological analysis in Control 2 (human foreskin-derived BJ iPSC-CMs). ( a ) Representative AP trace of ventricular-like control BJ iPSC-CMs obtained at 1 Hz of pacing. Inset . First derivative with respect to time (dV/dt). ( b–f) Action potential properties. Recordings at 1 and 2 Hz were similar to those obtained from the healthy donor patient derived-iPSC-CMs (Control 1). ( g-i) Current traces, I/V curves, and normalized current densities for NaV1.5, CaV1.2, and Kir2.1 ion channels, respectively. Data obtained from the control BJ iPSC-CMs (Control 2) were similar to the other control iPSC-CMs.

    Journal: bioRxiv

    Article Title: SNTA1 Gene Rescues Ion Channel Function in Cardiomyocytes Derived from Induced Pluripotent Stem Cells Reprogrammed from Muscular Dystrophy Patients with Arrhythmias

    doi: 10.1101/2022.01.25.477696

    Figure Lengend Snippet: Electrophysiological analysis in Control 2 (human foreskin-derived BJ iPSC-CMs). ( a ) Representative AP trace of ventricular-like control BJ iPSC-CMs obtained at 1 Hz of pacing. Inset . First derivative with respect to time (dV/dt). ( b–f) Action potential properties. Recordings at 1 and 2 Hz were similar to those obtained from the healthy donor patient derived-iPSC-CMs (Control 1). ( g-i) Current traces, I/V curves, and normalized current densities for NaV1.5, CaV1.2, and Kir2.1 ion channels, respectively. Data obtained from the control BJ iPSC-CMs (Control 2) were similar to the other control iPSC-CMs.

    Article Snippet: Rabbit anti-NaV 1.5 antibody (clone ASC-013, Alomone Labs) was used for NaV1.5 protein expression (1:500), mouse anti-Kir2.1 antibody (clone N112B/14, University of California at Davis/Nacional Institutes of Health 105 NeuroMab Facility) was used for Kir2.1 protein expression (1:500), mouse anti-Dystrophin (1:000, #D8043, Sigma) was used to detect the Dp427 dystrophin isoform.

    Techniques: Derivative Assay

    SCN5A , KCNJ2 and CACNA1C mRNA expression in control, DMD, and female iPSC-CMs. (a) SCN5A mRNA expression was increased in iPSC-CMs from hemizygous and heterozygous DMD individuals (top), as well as in the human left ventricle heart tissue from a Becker MD individual compared to a healthy subject (bottom). (b) KCNJ2 mRNA levels were higher in both hemizygous and heterozygous iPSC-CMs (top), like those found in human left ventricle heart tissue from Becker DM patients (bottom) when compared to the corresponding control. (c) CACNA1C mRNA expression was not significant different among tested groups from either iPSC-CMs (top) or left ventricle tissues (bottom). mRNA levels were determined by qRT-PCR and calculated by the comparative Ct method (2 -ddCt ) normalized to the internal control 18s rRNA. Errors bars represent SEM. The n -values are in parentheses. Two-tailed Mann-Whitney test. ** P

    Journal: bioRxiv

    Article Title: SNTA1 Gene Rescues Ion Channel Function in Cardiomyocytes Derived from Induced Pluripotent Stem Cells Reprogrammed from Muscular Dystrophy Patients with Arrhythmias

    doi: 10.1101/2022.01.25.477696

    Figure Lengend Snippet: SCN5A , KCNJ2 and CACNA1C mRNA expression in control, DMD, and female iPSC-CMs. (a) SCN5A mRNA expression was increased in iPSC-CMs from hemizygous and heterozygous DMD individuals (top), as well as in the human left ventricle heart tissue from a Becker MD individual compared to a healthy subject (bottom). (b) KCNJ2 mRNA levels were higher in both hemizygous and heterozygous iPSC-CMs (top), like those found in human left ventricle heart tissue from Becker DM patients (bottom) when compared to the corresponding control. (c) CACNA1C mRNA expression was not significant different among tested groups from either iPSC-CMs (top) or left ventricle tissues (bottom). mRNA levels were determined by qRT-PCR and calculated by the comparative Ct method (2 -ddCt ) normalized to the internal control 18s rRNA. Errors bars represent SEM. The n -values are in parentheses. Two-tailed Mann-Whitney test. ** P

    Article Snippet: Rabbit anti-NaV 1.5 antibody (clone ASC-013, Alomone Labs) was used for NaV1.5 protein expression (1:500), mouse anti-Kir2.1 antibody (clone N112B/14, University of California at Davis/Nacional Institutes of Health 105 NeuroMab Facility) was used for Kir2.1 protein expression (1:500), mouse anti-Dystrophin (1:000, #D8043, Sigma) was used to detect the Dp427 dystrophin isoform.

    Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test, MANN-WHITNEY

    Pruritogens influence NaV 1.9 currents.

    Journal: The Journal of Clinical Investigation

    Article Title: A disease mutation reveals a role for NaV1.9 in acute itch

    doi: 10.1172/JCI122481

    Figure Lengend Snippet: Pruritogens influence NaV 1.9 currents.

    Article Snippet: Primary antibodies used were rabbit anti-sfGFP (1:2,000; gift from Ramanujan Hegde), rabbit anti-NaV 1.9 (1:5,000; ASC-017, Alomone Labs), and rabbit anti-HSP90 (1:1,000; C45G5, Cell Signaling).

    Techniques:

    Pruritogens influence NaV 1.9 currents.

    Journal: The Journal of Clinical Investigation

    Article Title: A disease mutation reveals a role for NaV1.9 in acute itch

    doi: 10.1172/JCI122481

    Figure Lengend Snippet: Pruritogens influence NaV 1.9 currents.

    Article Snippet: Primary antibodies used were rabbit anti-sfGFP (1:2,000; gift from Ramanujan Hegde), rabbit anti-NaV 1.9 (1:5,000; ASC-017, Alomone Labs), and rabbit anti-HSP90 (1:1,000; C45G5, Cell Signaling).

    Techniques: