rabbit anti n wasp  (Santa Cruz Biotechnology)

 
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    Santa Cruz Biotechnology rabbit anti n wasp
    Rabbit Anti N Wasp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti n wasp antibodies  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit polyclonal anti n wasp antibodies
    Rabbit Polyclonal Anti N Wasp Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti n wasp  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit anti n wasp
    Rabbit Anti N Wasp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti rabbit n wasp antibody  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology anti rabbit n wasp antibody
    Anti Rabbit N Wasp Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    n wasp rabbit  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology n wasp rabbit
    N Wasp Rabbit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti n wasp h100  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit anti n wasp h100
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    rabbit anti n wasp  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit anti n wasp
    Rabbit Anti N Wasp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti n wasp antibody  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit anti n wasp antibody
    Rabbit Anti N Wasp Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti n wasp antibody  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit anti n wasp antibody
    <t>N-WASP</t> is recruited to rEtpE-C-coated-bead entry foci, and it interacts with rEtpE-C and native EtpE. (A) Spinning-disk confocal microscopy images at four different time points from the three-dimensional time-lapse video of RF/6A cells transfected with GFP–N-WASP (cyan) and then incubated with rEtpE-C-coated beads (red). xy planes show the upper surface of an RF/6A cell that had internalized rEtpE-C-coated beads. Each white arrow indicates a cluster of N-WASP. Cell surface extensions surrounding the cluster of beads are highlighted with yellow arrows in the side views ( xz and yz planes). Scale bar, 10 µm. (B) Affinity pulldown of THP-1 cell lysate incubated with rEtpE-C or rECH0825 bound to Ni-silica matrix. Bound proteins were eluted with imidazole and subjected to immunoblot analysis with <t>anti-N-WASP</t> and anti-His antibodies. One lane between rEtpE-C and rECH0825 was deleted to save the space without altering molecular mass or exposure time. (C) THP-1 cells were incubated with E. chaffeensis for 30 min, lysed, and immunoprecipitated (IP) with anti-EtpE-C antibody- or control IgG-bound protein A-agarose. The immunoprecipitates were subjected to immunoblotting with anti-N-WASP antibody. (B and C) Numbers to the left indicate kDa.
    Rabbit Anti N Wasp Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "EtpE Binding to DNase X Induces Ehrlichial Entry via CD147 and hnRNP-K Recruitment, Followed by Mobilization of N-WASP and Actin"

    Article Title: EtpE Binding to DNase X Induces Ehrlichial Entry via CD147 and hnRNP-K Recruitment, Followed by Mobilization of N-WASP and Actin

    Journal: mBio

    doi: 10.1128/mBio.01541-15

    N-WASP is recruited to rEtpE-C-coated-bead entry foci, and it interacts with rEtpE-C and native EtpE. (A) Spinning-disk confocal microscopy images at four different time points from the three-dimensional time-lapse video of RF/6A cells transfected with GFP–N-WASP (cyan) and then incubated with rEtpE-C-coated beads (red). xy planes show the upper surface of an RF/6A cell that had internalized rEtpE-C-coated beads. Each white arrow indicates a cluster of N-WASP. Cell surface extensions surrounding the cluster of beads are highlighted with yellow arrows in the side views ( xz and yz planes). Scale bar, 10 µm. (B) Affinity pulldown of THP-1 cell lysate incubated with rEtpE-C or rECH0825 bound to Ni-silica matrix. Bound proteins were eluted with imidazole and subjected to immunoblot analysis with anti-N-WASP and anti-His antibodies. One lane between rEtpE-C and rECH0825 was deleted to save the space without altering molecular mass or exposure time. (C) THP-1 cells were incubated with E. chaffeensis for 30 min, lysed, and immunoprecipitated (IP) with anti-EtpE-C antibody- or control IgG-bound protein A-agarose. The immunoprecipitates were subjected to immunoblotting with anti-N-WASP antibody. (B and C) Numbers to the left indicate kDa.
    Figure Legend Snippet: N-WASP is recruited to rEtpE-C-coated-bead entry foci, and it interacts with rEtpE-C and native EtpE. (A) Spinning-disk confocal microscopy images at four different time points from the three-dimensional time-lapse video of RF/6A cells transfected with GFP–N-WASP (cyan) and then incubated with rEtpE-C-coated beads (red). xy planes show the upper surface of an RF/6A cell that had internalized rEtpE-C-coated beads. Each white arrow indicates a cluster of N-WASP. Cell surface extensions surrounding the cluster of beads are highlighted with yellow arrows in the side views ( xz and yz planes). Scale bar, 10 µm. (B) Affinity pulldown of THP-1 cell lysate incubated with rEtpE-C or rECH0825 bound to Ni-silica matrix. Bound proteins were eluted with imidazole and subjected to immunoblot analysis with anti-N-WASP and anti-His antibodies. One lane between rEtpE-C and rECH0825 was deleted to save the space without altering molecular mass or exposure time. (C) THP-1 cells were incubated with E. chaffeensis for 30 min, lysed, and immunoprecipitated (IP) with anti-EtpE-C antibody- or control IgG-bound protein A-agarose. The immunoprecipitates were subjected to immunoblotting with anti-N-WASP antibody. (B and C) Numbers to the left indicate kDa.

    Techniques Used: Confocal Microscopy, Transfection, Incubation, Western Blot, Immunoprecipitation

    rabbit anti n wasp  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology rabbit anti n wasp
    Role of lumican on the expression and distribution of <t>N-WASP,</t> cortactin and sm α-actin in prostate cancer cells. Coverslips placed in 24 well polystyrene culture dishes were incubated in a solution of either 1 µg/mL collagen I or sequential 1 µg/mL collagen I and 10 µg/mL lumican. After culture dish treatment, 2 × 104 prostate cancer cells, PC3 (A) or DU145 (B), were seeded in RPMI containing L-glutamine/penicillin/streptomycin and 10% FBS, and incubated for 24 h at 37°C in a 5% CO2 humidified environment. A scratch was performed in the cells on the culture dish using a 100 µL pipette tip. Cells were washed three times with EBSS, maintained for a further 4 h (37°C, 5% CO2) and fixed for immunocytochemistry. The cells were labeled with <t>anti-N-WASP,</t> anti-sm α-actin, anti-cortactin and DAPI and analyzed using a Zeiss LSM510 scanning Confocal inverted microscope.
    Rabbit Anti N Wasp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Lumican expression, localization and antitumor activity in prostate cancer"

    Article Title: Lumican expression, localization and antitumor activity in prostate cancer

    Journal: Experimental cell research

    doi: 10.1016/j.yexcr.2013.01.023

    Role of lumican on the expression and distribution of N-WASP, cortactin and sm α-actin in prostate cancer cells. Coverslips placed in 24 well polystyrene culture dishes were incubated in a solution of either 1 µg/mL collagen I or sequential 1 µg/mL collagen I and 10 µg/mL lumican. After culture dish treatment, 2 × 104 prostate cancer cells, PC3 (A) or DU145 (B), were seeded in RPMI containing L-glutamine/penicillin/streptomycin and 10% FBS, and incubated for 24 h at 37°C in a 5% CO2 humidified environment. A scratch was performed in the cells on the culture dish using a 100 µL pipette tip. Cells were washed three times with EBSS, maintained for a further 4 h (37°C, 5% CO2) and fixed for immunocytochemistry. The cells were labeled with anti-N-WASP, anti-sm α-actin, anti-cortactin and DAPI and analyzed using a Zeiss LSM510 scanning Confocal inverted microscope.
    Figure Legend Snippet: Role of lumican on the expression and distribution of N-WASP, cortactin and sm α-actin in prostate cancer cells. Coverslips placed in 24 well polystyrene culture dishes were incubated in a solution of either 1 µg/mL collagen I or sequential 1 µg/mL collagen I and 10 µg/mL lumican. After culture dish treatment, 2 × 104 prostate cancer cells, PC3 (A) or DU145 (B), were seeded in RPMI containing L-glutamine/penicillin/streptomycin and 10% FBS, and incubated for 24 h at 37°C in a 5% CO2 humidified environment. A scratch was performed in the cells on the culture dish using a 100 µL pipette tip. Cells were washed three times with EBSS, maintained for a further 4 h (37°C, 5% CO2) and fixed for immunocytochemistry. The cells were labeled with anti-N-WASP, anti-sm α-actin, anti-cortactin and DAPI and analyzed using a Zeiss LSM510 scanning Confocal inverted microscope.

    Techniques Used: Expressing, Incubation, Transferring, Immunocytochemistry, Labeling, Inverted Microscopy

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    Santa Cruz Biotechnology rabbit anti n wasp
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