rabbit anti myelin basic protein  (Abcam)

 
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    Name:
    Anti Myelin Basic Protein antibody BDI221
    Description:

    Catalog Number:
    AB66188
    Price:
    None
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    Structured Review

    Abcam rabbit anti myelin basic protein
    <t>Myelin</t> <t>basic</t> <t>protein</t> labeling in cultured nodose ganglia. <t>Antibody</t> labeling for myelin basic protein (A), Hoechst staining of cell nuclei (B), and merged images (C) in nodose ganglia at baseline. Myelin basic protein and cell nuclei were detected after 6 (D-F) and 12 (G-I) months of culturing. Arrows highlight examples of myelin basic proteins. Scale bar = 25 μm. Abbreviations: MBP, myelin basic protein.

    https://www.bioz.com/result/rabbit anti myelin basic protein/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti myelin basic protein - by Bioz Stars, 2021-07
    93/100 stars

    Images

    1) Product Images from "Long-term culturing of porcine nodose ganglia"

    Article Title: Long-term culturing of porcine nodose ganglia

    Journal: Journal of neuroscience methods

    doi: 10.1016/j.jneumeth.2019.108546

    Myelin basic protein labeling in cultured nodose ganglia. Antibody labeling for myelin basic protein (A), Hoechst staining of cell nuclei (B), and merged images (C) in nodose ganglia at baseline. Myelin basic protein and cell nuclei were detected after 6 (D-F) and 12 (G-I) months of culturing. Arrows highlight examples of myelin basic proteins. Scale bar = 25 μm. Abbreviations: MBP, myelin basic protein.
    Figure Legend Snippet: Myelin basic protein labeling in cultured nodose ganglia. Antibody labeling for myelin basic protein (A), Hoechst staining of cell nuclei (B), and merged images (C) in nodose ganglia at baseline. Myelin basic protein and cell nuclei were detected after 6 (D-F) and 12 (G-I) months of culturing. Arrows highlight examples of myelin basic proteins. Scale bar = 25 μm. Abbreviations: MBP, myelin basic protein.

    Techniques Used: Labeling, Cell Culture, Antibody Labeling, Staining

    Related Articles

    Incubation:

    Article Title: Intrinsic mechanical sensitivity of auditory neurons as a contributor to sound-driven neural activity
    Article Snippet: The sections of 10 μm thickness were washed in PBS, and nonspecific binding was blocked with 1% bovine serum albumin (BSA) and 10% goat serum in PBS plus 0.1% Triton X-100 (PBST) for 1 hr. .. The primary antibodies, chicken anti-Tuj1 (Abcam), mouse anti-myelin basic protein (Abcam), and rabbit anti-Myo7a (Proteus Biosciences, Inc.), were incubated overnight at 4°C. .. After the incubation with the primary antibodies, the slides were washed three times with PBST and incubated with secondary antibodies for 1.5 hours at room temperature in the dark.

    Article Title: Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s disease
    Article Snippet: Sections prepared for detection of CNP, IBA-1 and Olig2 were pre-treated with citric acid sodium citrate buffer (pH 6.0) and sections prepared for the detection of Hu C/D were pre-treated with Tris–HCl (pH 8.0), respectively for 20 min at 90 °C. .. After washing, sections were incubated overnight at room temperature in a solution (phosphate-buffered saline with 2% BSA, 0.3% milk powder and 0.5% donkey serum) containing the following primary antibodies: (1) mouse anti-human neuronal protein HuCD (1:400, Thermo Fisher Scientific) for neurons, (2) rabbit anti IBA-1 (1:800, Wako) for microglial cells, (3) rabbit anti-GFAP (1:500, Dako) for astroglial cells, (4) rabbit anti-Olig2 (1:100, Immuno Biological Laboratories) for oligodendroglial cells, (5) mouse anti-CNP (1:300, BioLegend) for oligodendroglial cells, (6) goat anti-ferritin heavy chain Y-16 (1:200, Santa Cruz Biotechnology) for ferritin and (7) rat anti-myelin basic protein (1:400, abcam) for myelin. .. Subsequently, sections were rinsed in PBS-Tween (pH 7.4) and incubated in biotinylated secondary antibody solution (containing a mixture of PBS-T and phosphate-buffered saline with 2% BSA, 0.3% milk powder and 0.5% donkey serum, 2:1, for 1 h at room temperature) using donkey anti-mouse IgG, donkey anti-rat IgG, donkey anti-rabbit IgG and donkey anti-goat IgG (1:1000, Dianova, Germany).

    Article Title: Long-term culturing of porcine nodose ganglia
    Article Snippet: Tissues were then permeabilized in 0.15% Triton X-100, followed by blocking in PBS Superblock (ThermoFisher Scientific, Cat. #37515) containing 4% normal goat serum (Jackson Laboratories, Cat. # 005–000-121). .. Tissues were incubated with the following antibodies: rabbit anti-neurofilament heavy chain antibody (Novus Biologicals, Cat. # NB300–135); rabbit anti-myelin basic protein (abcam, Cat. # ab218011); rabbit anti-GFAP (Novus Biologicals, Cat. #NB300–141) at 1:1,000 dilution for 2 hours at 37 °C. .. Tissues were washed thoroughly in PBS and incubated with Alexa fluor 568-conjugated goat anti-rabbit secondary antibody (ThermoFisher, Cat. # A11011) at a 1:1,000 dilution for 1 hour at room temperature.

    Staining:

    Article Title: Health effects following subacute exposure to geogenic dust collected from active drainage surfaces (Nellis Dunes Recreation Area, Las Vegas, NV)
    Article Snippet: .. 2.9.7 Brain histology Two sections of cerebellum from each mouse brain, each 10 μm thick, were stained with either anti-CD3+ antibody (abcam, Cambridge, MA) or anti-myelin basic protein (MBP) antibody (abcam, Cambridge, MA). .. In sections stained with anti-CD3+, the number of T cells present throughout both sections was counted at 20X magnification.

    Article Title: Dimethyl Fumarate Suppresses Demyelination and Axonal Loss through Reduction in Pro-Inflammatory Macrophage-Induced Reactive Astrocytes and Complement C3 Deposition
    Article Snippet: .. Frozen spinal cord sections (cervical, thoracic, lumbar, and sacral regions) were cut at a thickness of 20 µm by cryostat and stained with rabbit anti-myelin basic protein (MBP) and rat anti-neurofilament monoclonal antibodies (Abcam, Cambridge, MA, USA) to study demyelination and axonal loss, respectively. .. Rabbit anti-Ym-1 Ab (Abcam, Cambridge, MA, USA) was used to investigate immunomodulatory macrophages, while rabbit anti-iNOS Ab (Abcam) was used as a marker for pro-inflammatory macrophages.

    SDS Page:

    Article Title: Blockage of NLRP3 inflammasome activation ameliorates acute inflammatory injury and long-term cognitive impairment induced by necrotizing enterocolitis in mice
    Article Snippet: Protein concentrations were determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). .. Thirty-five micrograms of total proteins was resolved by SDS-PAGE, transferred to 0.2 μm PVDF membranes, and probed using anti-NLRP3 (Abcam, Cambridge), anti-caspase-1 (Chemicon), anti-IL-1β (Cell Signaling), anti-myelin basic protein (MBP) (Abcam, Cambridge), anti-NeuN (Abcam, Cambridge), or anti-β-actin antibody (Sigma). .. Reactive signals were detected using chemiluminescence (Pierce, ECL Western Blotting Substrate, Thermo Fisher Scientific).

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  • 86
    Abcam anti myelin basic protein
    Expression of MBP in the medulla oblongata of the control, melatonin, CYP, CYP/melatonin groups at 7 and 21 PNDs. CYP administration dramatically downregulated the expression of MBP in rat medulla oblongata compared to the control group. Melatonin co-treatment significantly increased the expression of MBP. CYP, cypermethrin; MBP, <t>myelin</t> <t>basic</t> <t>protein;</t> PND, postnatal day. Scale bar=20 µm.
    Anti Myelin Basic Protein, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti myelin basic protein/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti myelin basic protein - by Bioz Stars, 2021-07
    86/100 stars
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    mbp  (Abcam)
    96
    Abcam mbp
    hBMSC-dSCs Myelinated Host Axons by Being Seeded into a Nerve Guide that Bridged a Critical Gap in a Rat Model of Sciatic Nerve Injury Longitudinal sections made in the mid-region of the sciatic nerve guide reveal the following. (A) Uni-axially aligned fibers immunopositive for rat <t>TUJ1,</t> representative of regrowing fibers. (B) Rows of Hoechst-stained nuclei between longitudinal layers immunopositive for human <t>MBP.</t> (C) Myelin-ensheathed axons and rows of peripherally located nuclei reminiscent of those of Schwann cells in the merged images of (A) and (B). (D) The myelin structure was illustrated in the TEM image of the transverse section (enlarged in d ∗ ). Scale bars, 50 μm for (A)–(C) and 200 nm for (D).
    Mbp, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbp/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mbp - by Bioz Stars, 2021-07
    96/100 stars
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    Image Search Results


    Expression of MBP in the medulla oblongata of the control, melatonin, CYP, CYP/melatonin groups at 7 and 21 PNDs. CYP administration dramatically downregulated the expression of MBP in rat medulla oblongata compared to the control group. Melatonin co-treatment significantly increased the expression of MBP. CYP, cypermethrin; MBP, myelin basic protein; PND, postnatal day. Scale bar=20 µm.

    Journal: Anatomy & Cell Biology

    Article Title: Effect of cypermethrin on the postnatal development of the medulla oblongata and the possible protective role of melatonin in albino rats

    doi: 10.5115/acb.20.193

    Figure Lengend Snippet: Expression of MBP in the medulla oblongata of the control, melatonin, CYP, CYP/melatonin groups at 7 and 21 PNDs. CYP administration dramatically downregulated the expression of MBP in rat medulla oblongata compared to the control group. Melatonin co-treatment significantly increased the expression of MBP. CYP, cypermethrin; MBP, myelin basic protein; PND, postnatal day. Scale bar=20 µm.

    Article Snippet: Following the blocking of endogenous peroxidase activity with 3% H2 O2 in methanol and nonspecific binding sites with a protein blocker, the primary antibody anti Mab2 (Mouse monoclonal, 1:500, Abcam, Cambridge, UK), anti-myelin basic protein (MBP; Rabbit polyclonal, 1:300, Abcam), anti-oligodendrocyte transcription factor (anti-olig2; Rabbit monoclonal, 1:100; Abcam) and anti-GFAP (Rabbit polyclonal, 1:300, LabVision, Fermont, CA, USA) added with overnight incubation in a cold room.

    Techniques: Expressing

    Area percentage of (A) MAP-2, (B) MBP and (C) GFAP and (D) Olig2+ve cells in the medulla oblongata of the control, melatonin, CYP, CYP/melatonin groups at 7 and 21 PNDs. CYP, cypermethrin; GFAP, glial fibrillary acidic protein; MAP-2, microtubule-associated protein-2; MBP, myelin basic protein; Olig2, oligodendrocyte transcription factor; PND, postnatal day. ** P

    Journal: Anatomy & Cell Biology

    Article Title: Effect of cypermethrin on the postnatal development of the medulla oblongata and the possible protective role of melatonin in albino rats

    doi: 10.5115/acb.20.193

    Figure Lengend Snippet: Area percentage of (A) MAP-2, (B) MBP and (C) GFAP and (D) Olig2+ve cells in the medulla oblongata of the control, melatonin, CYP, CYP/melatonin groups at 7 and 21 PNDs. CYP, cypermethrin; GFAP, glial fibrillary acidic protein; MAP-2, microtubule-associated protein-2; MBP, myelin basic protein; Olig2, oligodendrocyte transcription factor; PND, postnatal day. ** P

    Article Snippet: Following the blocking of endogenous peroxidase activity with 3% H2 O2 in methanol and nonspecific binding sites with a protein blocker, the primary antibody anti Mab2 (Mouse monoclonal, 1:500, Abcam, Cambridge, UK), anti-myelin basic protein (MBP; Rabbit polyclonal, 1:300, Abcam), anti-oligodendrocyte transcription factor (anti-olig2; Rabbit monoclonal, 1:100; Abcam) and anti-GFAP (Rabbit polyclonal, 1:300, LabVision, Fermont, CA, USA) added with overnight incubation in a cold room.

    Techniques:

    hBMSC-dSCs Myelinated Host Axons by Being Seeded into a Nerve Guide that Bridged a Critical Gap in a Rat Model of Sciatic Nerve Injury Longitudinal sections made in the mid-region of the sciatic nerve guide reveal the following. (A) Uni-axially aligned fibers immunopositive for rat TUJ1, representative of regrowing fibers. (B) Rows of Hoechst-stained nuclei between longitudinal layers immunopositive for human MBP. (C) Myelin-ensheathed axons and rows of peripherally located nuclei reminiscent of those of Schwann cells in the merged images of (A) and (B). (D) The myelin structure was illustrated in the TEM image of the transverse section (enlarged in d ∗ ). Scale bars, 50 μm for (A)–(C) and 200 nm for (D).

    Journal: Stem Cell Reports

    Article Title: Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells

    doi: 10.1016/j.stemcr.2017.08.004

    Figure Lengend Snippet: hBMSC-dSCs Myelinated Host Axons by Being Seeded into a Nerve Guide that Bridged a Critical Gap in a Rat Model of Sciatic Nerve Injury Longitudinal sections made in the mid-region of the sciatic nerve guide reveal the following. (A) Uni-axially aligned fibers immunopositive for rat TUJ1, representative of regrowing fibers. (B) Rows of Hoechst-stained nuclei between longitudinal layers immunopositive for human MBP. (C) Myelin-ensheathed axons and rows of peripherally located nuclei reminiscent of those of Schwann cells in the merged images of (A) and (B). (D) The myelin structure was illustrated in the TEM image of the transverse section (enlarged in d ∗ ). Scale bars, 50 μm for (A)–(C) and 200 nm for (D).

    Article Snippet: Immunofluorescence Cells were incubated (16 hr, 4°C) with one of the primary antibodies against CD 73 (mouse monoclonal, Abcam, cat. no. ab54217, 1:500), CD 90 (mouse monoclonal, Abcam, cat. no. ab181469, 1:200), CD105 (mouse monoclonal, Abcam, cat. no. ab2529, 1:200), nestin (mouse monoclonal, Abcam, cat. no. ab22035, 1:200), GFAP (mouse monoclonal, Abcam, cat. no. ab49874, 1:1,000), S100 (rabbit monoclonal, Abcam, cat. no. ab52642, 1:100), p75NTR (rabbit polyclonal, Abcam, cat. no. ab8874, 1:500), TUJ1 (rabbit polyclonal, Biolegend, cat. no. 802001, 1:1,000) and MBP (rabbit polyclonal, Abcam, cat. no. ab124493, 1:100).

    Techniques: Staining, Transmission Electron Microscopy

    In Vitro Myelination of the DRG Neuritic Network by hBMSC-dSCs (A) Phase-contrast image showing hBMSC-dSCs (arrows) associated with neurons as early as 48 hr in co-culture with the neuritic network of purified DRG neurons in neuron maintenance medium (a). Immunofluorescence for S100 and TUJ1 in a parallel culture showing hBMSC-dSC (arrows) abutting on the neurites (b; right panels, zoom-in views of the boxed areas i–iii). Following 14 days of myelination induction, myelin-like segments (double-headed arrows) were formed by hBMSC-dSCs along the neuritic networks as shown by phase contrast (c) and immunofluorescence for MBP (d). Scale bar, 100 μm. (B) hBMSCs in parallel co-culture with DRG neurons (arrows) showed a fibroblast-like morphology (a) and failed to form MBP-positive segments along neurites (b). Scale bar, 100 μm. (C) SCLCs in parallel co-culture with the neuritic network of DRG neurons (arrows) reverted to the myofibroblast phenotype (a) and failed to form MBP-positive segments along neurites (b). Scale bar, 100 μm. (D) Histogram showing myelinated segment counts in ten fields for hBMSC-dSC versus hardly any for hBMSC ( ∗∗ p

    Journal: Stem Cell Reports

    Article Title: Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells

    doi: 10.1016/j.stemcr.2017.08.004

    Figure Lengend Snippet: In Vitro Myelination of the DRG Neuritic Network by hBMSC-dSCs (A) Phase-contrast image showing hBMSC-dSCs (arrows) associated with neurons as early as 48 hr in co-culture with the neuritic network of purified DRG neurons in neuron maintenance medium (a). Immunofluorescence for S100 and TUJ1 in a parallel culture showing hBMSC-dSC (arrows) abutting on the neurites (b; right panels, zoom-in views of the boxed areas i–iii). Following 14 days of myelination induction, myelin-like segments (double-headed arrows) were formed by hBMSC-dSCs along the neuritic networks as shown by phase contrast (c) and immunofluorescence for MBP (d). Scale bar, 100 μm. (B) hBMSCs in parallel co-culture with DRG neurons (arrows) showed a fibroblast-like morphology (a) and failed to form MBP-positive segments along neurites (b). Scale bar, 100 μm. (C) SCLCs in parallel co-culture with the neuritic network of DRG neurons (arrows) reverted to the myofibroblast phenotype (a) and failed to form MBP-positive segments along neurites (b). Scale bar, 100 μm. (D) Histogram showing myelinated segment counts in ten fields for hBMSC-dSC versus hardly any for hBMSC ( ∗∗ p

    Article Snippet: Immunofluorescence Cells were incubated (16 hr, 4°C) with one of the primary antibodies against CD 73 (mouse monoclonal, Abcam, cat. no. ab54217, 1:500), CD 90 (mouse monoclonal, Abcam, cat. no. ab181469, 1:200), CD105 (mouse monoclonal, Abcam, cat. no. ab2529, 1:200), nestin (mouse monoclonal, Abcam, cat. no. ab22035, 1:200), GFAP (mouse monoclonal, Abcam, cat. no. ab49874, 1:1,000), S100 (rabbit monoclonal, Abcam, cat. no. ab52642, 1:100), p75NTR (rabbit polyclonal, Abcam, cat. no. ab8874, 1:500), TUJ1 (rabbit polyclonal, Biolegend, cat. no. 802001, 1:1,000) and MBP (rabbit polyclonal, Abcam, cat. no. ab124493, 1:100).

    Techniques: In Vitro, Co-Culture Assay, Purification, Immunofluorescence

    SLDS inhibits inflammatory cytokine secretion, increases phagocytosis in primary microglia, and promotes primary oligodendrocyte differentiation via M2 polarization. The M1 phenotype was induced using LPS plus IFN-γ, and the M2 phenotype was induced using IL-4 plus IL-13. Vehicle was added to non-treated microglia. a ELISA results indicated that SLDS inhibited the expression of IL-1β, IL-2, IL-6, IL-8, and TNFα in microglial-conditioned media; n = 9–12 per group. b Quantification of fluorescent microsphere intensity; n = 9 per group. c Representative images of microglial phagocytosis detected by fluorescent microspheres. Left scale bar, 10 μm; right scale bar, 5 μm. Phalloidin staining was used to visualize F-actin. d In vitro experiments using the transwell contact-independent system. Microglia seeded in inserts were incubated with vehicle or different SLDS treatment combinations for 48 h, after which the inserts were placed over oligodendrocyte cultures. After 3 days in culture, inserts were changed and fresh 48-h-treated microglia inserts were added. Oligodendrocytes were collected 5 or 7 days after the initial microglial insert addition. Vehicle was added to oligodendrocytes alone (control group) or oligodendrocyte-microglia cocultures (M0 group). e The expression of NG2 and MBP in the oligodendrocytes cocultured with microglia treated with various concentrations of SLDS (and controls). RT-PCR was performed to detect the expression of NG2 and MBP; n = 3 per group. f Representative NG2 (green) and MBP (red) staining in the different groups. Scale bar, 50 μm. DAPI (blue) was used as a nuclear marker. Data are expressed as mean ± SEM. * P

    Journal: Journal of Neuroinflammation

    Article Title: Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia

    doi: 10.1186/s12974-018-1081-0

    Figure Lengend Snippet: SLDS inhibits inflammatory cytokine secretion, increases phagocytosis in primary microglia, and promotes primary oligodendrocyte differentiation via M2 polarization. The M1 phenotype was induced using LPS plus IFN-γ, and the M2 phenotype was induced using IL-4 plus IL-13. Vehicle was added to non-treated microglia. a ELISA results indicated that SLDS inhibited the expression of IL-1β, IL-2, IL-6, IL-8, and TNFα in microglial-conditioned media; n = 9–12 per group. b Quantification of fluorescent microsphere intensity; n = 9 per group. c Representative images of microglial phagocytosis detected by fluorescent microspheres. Left scale bar, 10 μm; right scale bar, 5 μm. Phalloidin staining was used to visualize F-actin. d In vitro experiments using the transwell contact-independent system. Microglia seeded in inserts were incubated with vehicle or different SLDS treatment combinations for 48 h, after which the inserts were placed over oligodendrocyte cultures. After 3 days in culture, inserts were changed and fresh 48-h-treated microglia inserts were added. Oligodendrocytes were collected 5 or 7 days after the initial microglial insert addition. Vehicle was added to oligodendrocytes alone (control group) or oligodendrocyte-microglia cocultures (M0 group). e The expression of NG2 and MBP in the oligodendrocytes cocultured with microglia treated with various concentrations of SLDS (and controls). RT-PCR was performed to detect the expression of NG2 and MBP; n = 3 per group. f Representative NG2 (green) and MBP (red) staining in the different groups. Scale bar, 50 μm. DAPI (blue) was used as a nuclear marker. Data are expressed as mean ± SEM. * P

    Article Snippet: Slides or glass coverslips were washed in PBS and immersed in monkey serum (Jackson Immuno Research Laboratories Inc., West Grove, PA, USA) for 30 min. Primary antibodies included the following: rabbit anti-MAP2 (sc-20172, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rat anti-CD16/32 (553142, BD, Franklin Lakes, NJ, USA), goat anti-CD206 (AF2535, R & D Systems, Minneapolis, USA), rabbit anti-inducible nitric oxide synthase (iNOS, ab15323, Abcam, San Francisco, CA, USA), goat anti-Arg1 (sc-18351, Santa Cruz Biotechnology), rabbit anti-Iba1 (019-19741, Wako, Osaka, Japan), mouse anti-NG2 (MAB5384, Millipore, Billerica, MA, USA), and rabbit anti-MBP (ab40390, Abcam).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Staining, In Vitro, Incubation, Reverse Transcription Polymerase Chain Reaction, Marker

    SLDS inhibits inflammatory cytokine secretion, increases phagocytosis in primary microglia, and promotes primary oligodendrocyte differentiation via M2 polarization. The M1 phenotype was induced using LPS plus IFN-γ, and the M2 phenotype was induced using IL-4 plus IL-13. Vehicle was added to non-treated microglia. a ELISA results indicated that SLDS inhibited the expression of IL-1β, IL-2, IL-6, IL-8, and TNFα in microglial-conditioned media; n = 9–12 per group. b Quantification of fluorescent microsphere intensity; n = 9 per group. c Representative images of microglial phagocytosis detected by fluorescent microspheres. Left scale bar, 10 μm; right scale bar, 5 μm. Phalloidin staining was used to visualize F-actin. d In vitro experiments using the transwell contact-independent system. Microglia seeded in inserts were incubated with vehicle or different SLDS treatment combinations for 48 h, after which the inserts were placed over oligodendrocyte cultures. After 3 days in culture, inserts were changed and fresh 48-h-treated microglia inserts were added. Oligodendrocytes were collected 5 or 7 days after the initial microglial insert addition. Vehicle was added to oligodendrocytes alone (control group) or oligodendrocyte-microglia cocultures (M0 group). e The expression of NG2 and MBP in the oligodendrocytes cocultured with microglia treated with various concentrations of SLDS (and controls). RT-PCR was performed to detect the expression of NG2 and MBP; n = 3 per group. f Representative NG2 (green) and MBP (red) staining in the different groups. Scale bar, 50 μm. DAPI (blue) was used as a nuclear marker. Data are expressed as mean ± SEM. * P

    Journal: Journal of Neuroinflammation

    Article Title: Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia

    doi: 10.1186/s12974-018-1081-0

    Figure Lengend Snippet: SLDS inhibits inflammatory cytokine secretion, increases phagocytosis in primary microglia, and promotes primary oligodendrocyte differentiation via M2 polarization. The M1 phenotype was induced using LPS plus IFN-γ, and the M2 phenotype was induced using IL-4 plus IL-13. Vehicle was added to non-treated microglia. a ELISA results indicated that SLDS inhibited the expression of IL-1β, IL-2, IL-6, IL-8, and TNFα in microglial-conditioned media; n = 9–12 per group. b Quantification of fluorescent microsphere intensity; n = 9 per group. c Representative images of microglial phagocytosis detected by fluorescent microspheres. Left scale bar, 10 μm; right scale bar, 5 μm. Phalloidin staining was used to visualize F-actin. d In vitro experiments using the transwell contact-independent system. Microglia seeded in inserts were incubated with vehicle or different SLDS treatment combinations for 48 h, after which the inserts were placed over oligodendrocyte cultures. After 3 days in culture, inserts were changed and fresh 48-h-treated microglia inserts were added. Oligodendrocytes were collected 5 or 7 days after the initial microglial insert addition. Vehicle was added to oligodendrocytes alone (control group) or oligodendrocyte-microglia cocultures (M0 group). e The expression of NG2 and MBP in the oligodendrocytes cocultured with microglia treated with various concentrations of SLDS (and controls). RT-PCR was performed to detect the expression of NG2 and MBP; n = 3 per group. f Representative NG2 (green) and MBP (red) staining in the different groups. Scale bar, 50 μm. DAPI (blue) was used as a nuclear marker. Data are expressed as mean ± SEM. * P

    Article Snippet: Slides or glass coverslips were washed in PBS and immersed in monkey serum (Jackson Immuno Research Laboratories Inc., West Grove, PA, USA) for 30 min. Primary antibodies included the following: rabbit anti-MAP2 (sc-20172, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rat anti-CD16/32 (553142, BD, Franklin Lakes, NJ, USA), goat anti-CD206 (AF2535, R & D Systems, Minneapolis, USA), rabbit anti-inducible nitric oxide synthase (iNOS, ab15323, Abcam, San Francisco, CA, USA), goat anti-Arg1 (sc-18351, Santa Cruz Biotechnology), rabbit anti-Iba1 (019-19741, Wako, Osaka, Japan), mouse anti-NG2 (MAB5384, Millipore, Billerica, MA, USA), and rabbit anti-MBP (ab40390, Abcam).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Staining, In Vitro, Incubation, Reverse Transcription Polymerase Chain Reaction, Marker