Journal: Translational Lung Cancer Research
Article Title: SRC and PIM1 as potential co-targets to overcome resistance in MET deregulated non-small cell lung cancer
doi: 10.21037/tlcr-20-681
Figure Lengend Snippet: Primary and secondary antibodies used in the study
Article Snippet: Primary antibodies and secondary antibodies used are in . table ft1 table-wrap mode="anchored" t5 caption a7 Western blotting primary antibody Dilution Company and catalog number Mouse anti-beta-actin 1:5,000 Sigma Aldrich (#A5441) Rabbit anti-AKT 1:1,000 Cell signaling (#9272) Rabbit anti-Phospho-AKT (S473) 1:1,000 Cell signaling (#9271) Rabbit anti-AXL 1:1,000 Cell signaling (#8661) Rabbit anti-CDCP1 1:1,000 Cell signaling (#4115) Rabbit anti-Phospho-CDCP1 (Y707) 1:1,000 Cell signaling (#13111) Rabbit anti-EGFR 1:1,000 Cell signaling (#4267) Rabbit anti-Phospho-EGFR (Y1068) 1:1,000 Cell signaling (#3777) Rabbit anti-Phospho-EGFR (Y845) 1:1,000 Cell signaling (#6963) Rabbit anti-ERK1/2 1:1,000 Cell signaling (#9102) Rabbit anti-PhosphoERK1/2 (T202/Y204) 1:1,000 Cell signaling (#9101) Rabbit anti-cMET 1:1,000 Cell signaling (#8198) Rabbit anti-Phospho-cMET (Y1234-1235) 1:1,000 Cell signaling (#3077) Rabbit anti-Src 1:1,000 Cell signaling (#2109) Rabbit anti-Phospho-Src Family (Y416) 1:1,000 Cell signaling (#6943) Mouse anti-STAT3 1:1,000 Cell signaling (#9139) Rabbit anti-Phospho STAT3 (Y705) 1:1,000 Cell signaling (#9145) Mouse anti-YAP1 1:1,000 Cell signaling (#12395) Rabbit anti-Phospho YAP1 (Y357) 1:1,000 Abcam (#ab62751) Rabbit anti-AXL 1:1,000 Cell signaling (#8661) Donkey anti-Rabbit IgG HRP-linked 1:5,000 GE Healthcare (#NA934) Sheep anti-Mouse IgG HRP-linked 1:5,000 GE Healthcare (#NXA931) Open in a separate window caption a8 Primary and secondary antibodies used in the study Cell viability assay The EBC-1 human squamous lung cancer cell line with MET amplification, the Hs746T human gastric cancer cell line with MET exon 14 skipping mutation, and the H1993 human lung adenocarcinoma cell line harboring MET amplification were purchased from American Type Culture Collection (ATCC).
Techniques: