rabbit anti-mouse lyve1 polyclonal antibody 11-034  (AngioBio Inc)

Journal: eLife

Article Title: Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta

doi: 10.7554/eLife.52690

Figure Lengend Snippet: ( A ) Aggregated E9.5 to E13.5 scRNA-seq data from is shown for Lyve1 . Red arrow indicates normalized expression in endothelial cells. ( B ) Sagittal cryosection (14 µM) of an E11.5 S1PR1-GS mouse embryo immunostained for LYVE1, CD31, and ITGA6. The boxed region is shown at higher magnification. Scale bar is 200 µM; CV – cardinal vein.

Article Snippet: Antibody , Anti-mouse LYVE1 (rabbit Polyclonal) , ReliaTech , Cat #: 103-PA50AG RRID: AB_2783787 , 1:300 , .

Techniques: Expressing

Journal: eLife

Article Title: Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta

doi: 10.7554/eLife.52690

Figure Lengend Snippet: ( A ) Representative confocal image of a sagittal cryosection (14 µM) of a S1RP1-GS mouse aorta immunostained for VEC and lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE1) (N = 2). White arrows indicate GFP+ arterial ECs at a branch point orifices and yellow arrows indicate adventitia-associated GFP+ LECs. Scale bar is 50 µM. ( B ) Confocal image of a S1RP1-GS mouse thoracic aorta whole-mount preparation immunostained for vascular endothelial growth factor receptor 3 (VEGFR3; Flt4 ), LYVE1, and CLDN5 with the tunica intima in contact with coverslip. Orange stars indicate VEGFR3+LYVE1+GFP+ areas, cyan stars indicate VEGFR3+LYVE1 low GFP+ areas, and magenta stars indicate VEGFR3+LYVE1+GFP- areas. Image is representative of observations from three mice (see also ). Scale bar is 100 µM. ( C–D ) Representative confocal images of mesenteric lymphatics from H2B-GFP control ( C ) and S1PR1-GS mice bearing Sphk2 -/- : Sphk1 f/f (Cre-) or Sphk2 -/- : Sphk1 f/f : Lyve1-Cre (Lyve1-Cre+) alleles whole-mounted and immunostained for PROX1 and ASMA. White arrows indicate ASMA+PROX1+GFP+, yellow arrows indicate PROX1+GFP-, cyan arrows indicate ASMA+PROX1+GFP-. Scale bars are 100 µM. ( E ) Quantification of GFP+ signal over ASMA+PROX1+ and ASMA-PROX1+ areas from Cre- (n = 5), Lyve1-Cre (n = 4), and H2B-GFP control (n = 2) mice. One-way ANOVA followed by Bonferroni’s post hoc test, **** P < 0.0001.

Article Snippet: Antibody , Anti-mouse LYVE1 (rabbit Polyclonal) , ReliaTech , Cat #: 103-PA50AG RRID: AB_2783787 , 1:300 , .

Techniques: Control

Journal: eLife

Article Title: Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta

doi: 10.7554/eLife.52690

Figure Lengend Snippet: ( A ) Barplots illustrate transcript counts of Flt4 (VEGFR3), Lyve1 , Ccl21 , and Itga6 in each of the nine clusters identified by scRNA-seq analysis. Each bar represents a single cell, either GFP high (green) or GFP low (blue). ( B ) Confocal image of a S1RP1-GS mouse thoracic aorta whole-mount preparation immunostained for VEGFR3 and LYVE1 with the tunica adventitia in contact with coverslip. Orange stars indicate VEGFR3+LYVE1+GFP+ areas, cyan stars indicate VEGFR3+LYVE1 low GFP+ areas, and magenta stars indicate VEGFR3+LYVE1+GFP- areas. ( C ) Immunostaining of a sagittal section of a S1PR1-GS mouse thoracic aorta for ITGA6 and LYVE1. White arrows indicate GFP+ITGA6+LYVE1- arterial ECs of the branch point orifice. The yellow arrow indicates a GFP+ ECs associated with an adventitial LYVE1+ITGA6+ structure. Scale bars are 50 µM.

Article Snippet: Antibody , Anti-mouse LYVE1 (rabbit Polyclonal) , ReliaTech , Cat #: 103-PA50AG RRID: AB_2783787 , 1:300 , .

Techniques: Immunostaining

Journal: eLife

Article Title: Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta

doi: 10.7554/eLife.52690

Figure Lengend Snippet: ( A ) Pie-chart distribution of S1pr1 ECKO MAECs up-regulated transcripts. The 258 transcripts were binned according to their cluster assignment from scRNA-seq analysis. The blue and cyan numbers indicate the total percentage of transcripts enriched in LEC and LEC plus at least one cluster, respectively. The numbers in parentheses are absolute transcripts numbers. (See ) ( B ) Heatmap (row Z-scores) of all 78 S1pr1 ECKO MAECs up-regulated transcripts that are LEC-enriched. Selected GSEA pathways are identified in the top heatmap. (See ) ( C ) Confocal images of two fields ( c’ and c’’ ) of a whole-mounted S1RP1-GS mouse thoracic aorta immunostained for LYVE1 and C-C motif chemokine 21 (CCL21). The tunica adventitia was facing the coverslip. ( D ) Confocal images of a sagittal cryosection (14 µM) of an S1RP1-GS mouse thoracic aorta immunostained CCL21 and LYVE1. Yellow arrows indicate adventitia-associated CCL21+LYVE1+GFP+ cells and the white arrow indicate GFP+LYVE1- cells at a branch. Scale bars are 50 µM.

Article Snippet: Antibody , Anti-mouse LYVE1 (rabbit Polyclonal) , ReliaTech , Cat #: 103-PA50AG RRID: AB_2783787 , 1:300 , .

Techniques:

Journal: eLife

Article Title: Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta

doi: 10.7554/eLife.52690

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-mouse LYVE1 (rabbit Polyclonal) , ReliaTech , Cat #: 103-PA50AG RRID: AB_2783787 , 1:300 , .

Techniques: Sequencing, Purification, Agarose Gel Electrophoresis, Software