Structured Review

Millipore rabbit anti mouse dcn
Schematic diagram of the <t>DCN-mediated</t> translational regulation of <t>fibrillin-1</t> in NRK cells. Further details are given in Results.
Rabbit Anti Mouse Dcn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti mouse dcn/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti mouse dcn - by Bioz Stars, 2021-05
99/100 stars

Images

1) Product Images from "Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin"

Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin

Journal:

doi: 10.2353/ajpath.2007.060497

Schematic diagram of the DCN-mediated translational regulation of fibrillin-1 in NRK cells. Further details are given in Results.
Figure Legend Snippet: Schematic diagram of the DCN-mediated translational regulation of fibrillin-1 in NRK cells. Further details are given in Results.

Techniques Used:

Decorin and IGF-I induce fibrillin-1 protein synthesis in normal rat kidney fibroblasts via the IGF-IR. A and B show representative Western blots for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I
Figure Legend Snippet: Decorin and IGF-I induce fibrillin-1 protein synthesis in normal rat kidney fibroblasts via the IGF-IR. A and B show representative Western blots for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I

Techniques Used: Western Blot, Incubation, Recombinant

DCN- and IGF-I-dependent regulation of fibrillin-1 in NRK cells involves mTOR. A- - C: DCN- and IGF-I-mediated phosphorylation of mTOR in NRK cells (8 minutes of incubation) was suppressed by inhibition of IGF-IR ( A ), by an inhibition of PI3K ( B ), and by
Figure Legend Snippet: DCN- and IGF-I-dependent regulation of fibrillin-1 in NRK cells involves mTOR. A- - C: DCN- and IGF-I-mediated phosphorylation of mTOR in NRK cells (8 minutes of incubation) was suppressed by inhibition of IGF-IR ( A ), by an inhibition of PI3K ( B ), and by

Techniques Used: Incubation, Inhibition

Involvement of the PI3K/Akt pathway in DCN- and IGF-I-mediated regulation of fibrillin-1 in NRK cells. A: Western blot for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I in the presence or absence
Figure Legend Snippet: Involvement of the PI3K/Akt pathway in DCN- and IGF-I-mediated regulation of fibrillin-1 in NRK cells. A: Western blot for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I in the presence or absence

Techniques Used: Western Blot, Incubation, Recombinant

Decorin deficiency in STZ-induced diabetes is associated with lower accumulation of fibrillin-1 and overexpression of IGF-IR. A and C: Western blots for fibrillin-1 ( A ), IGF-IR ( C ), and β-tubulin in homogenates of diabetic kidneys from Dcn −/−
Figure Legend Snippet: Decorin deficiency in STZ-induced diabetes is associated with lower accumulation of fibrillin-1 and overexpression of IGF-IR. A and C: Western blots for fibrillin-1 ( A ), IGF-IR ( C ), and β-tubulin in homogenates of diabetic kidneys from Dcn −/−

Techniques Used: Over Expression, Western Blot

Related Articles

Incubation:

Article Title: Inhibition of PbGP43 Expression May Suggest that gp43 is a Virulence Factor in Paracoccidioides brasiliensis
Article Snippet: Blotted proteins were assayed for laminin and fibronectin affinity as follows: the membranes were incubated with 1% bovine serum albumin (BSA) in PBS, pH 7.4, for 4 h at room temperature and then for 90 min in PBS-T-BSA (PBS, 0,05% Tween 20, 5% non fat milk) containing 30 µg/ml of either laminin (derived from Engelbreth-Holm-Swarm murine sarcoma from Sigma) or human fibronectin (Sigma). .. Membranes were washed three times in PBS-T, 10 min each, and then incubated for 1 h under agitation with rabbit anti-mouse laminin or rabbit anti-human fibronectin antibodies (both from Sigma, 1∶100) in PBS-T-BSA under continuous agitation. .. The blots were washed in PBS-T and incubated with peroxidase-labeled goat anti-rabbit immunoglobulin at 1∶1,000 in PBS-T-BSA at room temperature.

Article Title: The PDZ Protein GIPC Regulates Trafficking of the LPA1 Receptor from APPL Signaling Endosomes and Attenuates the Cell's Response to LPA
Article Snippet: Insoluble material was removed by centrifugation (10,000×g for 30 min at 4°C), and the protein concentration of the supernatant was determined by Bradford assay (Bio-Rad Laboratories, Hercules, CA). .. Cell lysates (3–4 mg protein) were incubated at 4°C with mouse anti-FLAG IgG overnight followed by incubation with protein G-Sepharose beads (Sigma-Aldrich) for 1 h. Beads were then washed extensively with lysis buffer and resuspended in Laemmli sample buffer (50 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol and 0.01% bromophenol blue) for SDS-PAGE. .. Immunoblotting Proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Billerica, MA).

Article Title: Two RSV Platforms for G, F, or G+F Proteins VLPs
Article Snippet: F, Ga, and Gb Antibody EIAs The secreted form of F, Ga, or Gb protein antigens was produced from stably-transfected 293F cells in serum-free media and coated onto a 96-well microtiter plate in coating buffer (15 mM Na2 CO3 , 35 mM NaHCO3 , 3 mM NaN3 , pH 9.4–9.6). .. The plates were incubated in 2% nonfat dry milk dissolved in PBS blocking solution for 2 h at 37 °C, washed with PBS-T (PBS + 0.15% Tween-20), and serum specimens at 1:200 dilution added to the wells, incubated for 1 h at 37 °C, the plates washed with PBS-T, and goat anti mouse IgG-HRP (1:5000) added and incubated for 1 h at 37 °C. .. Color was developed with o -Phenylenediamine dihydrochloride (OPD) (Sigma-Aldrich, St. Louis, MO, USA) substrate and the reaction stopped after 30 min at RT with 4N H2 SO4 .

Lysis:

Article Title: The PDZ Protein GIPC Regulates Trafficking of the LPA1 Receptor from APPL Signaling Endosomes and Attenuates the Cell's Response to LPA
Article Snippet: Insoluble material was removed by centrifugation (10,000×g for 30 min at 4°C), and the protein concentration of the supernatant was determined by Bradford assay (Bio-Rad Laboratories, Hercules, CA). .. Cell lysates (3–4 mg protein) were incubated at 4°C with mouse anti-FLAG IgG overnight followed by incubation with protein G-Sepharose beads (Sigma-Aldrich) for 1 h. Beads were then washed extensively with lysis buffer and resuspended in Laemmli sample buffer (50 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol and 0.01% bromophenol blue) for SDS-PAGE. .. Immunoblotting Proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Billerica, MA).

SDS Page:

Article Title: The PDZ Protein GIPC Regulates Trafficking of the LPA1 Receptor from APPL Signaling Endosomes and Attenuates the Cell's Response to LPA
Article Snippet: Insoluble material was removed by centrifugation (10,000×g for 30 min at 4°C), and the protein concentration of the supernatant was determined by Bradford assay (Bio-Rad Laboratories, Hercules, CA). .. Cell lysates (3–4 mg protein) were incubated at 4°C with mouse anti-FLAG IgG overnight followed by incubation with protein G-Sepharose beads (Sigma-Aldrich) for 1 h. Beads were then washed extensively with lysis buffer and resuspended in Laemmli sample buffer (50 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol and 0.01% bromophenol blue) for SDS-PAGE. .. Immunoblotting Proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Billerica, MA).

FLAG-tag:

Article Title: Protein Interaction Analysis of Senataxin and the ALS4 L389S Mutant Yields Insights into Senataxin Post-Translational Modification and Uncovers Mutant-Specific Binding with a Brain Cytoplasmic RNA-Encoded Peptide
Article Snippet: Protein lysates were resolved using NuPAGE® Tris-Acetate gradient Gels (Invitrogen) and transferred to PVDF membrane (Sigma) by 1 hr of electroblot at 30 volts and blocked with 4% non-fat dry milk. .. To detect FLAG epitope, anti-FLAG M2 antibody (Sigma: F1804) was used (1∶5,000) in conjunction with anti-mouse HRP secondary antibodies (Sigma: A9044) (1∶10,000). .. The ECL Plus HRP detection kit (Amersham) was used for chemiluminescent detection.

Western Blot:

Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin
Article Snippet: TRIzol, reagents for reverse transcriptase-polymerase chain reaction (RT-PCR), cell culture media, and sera were obtained from Invitrogen (Groningen, The Netherlands), and tissue culture plastic was from Falcon (Becton-Dickinson, Heidelberg, Germany). .. Antibodies used for Western blots were as follows: rabbit anti-human fibrillin-1, rabbit-anti-human DCN, rabbit anti-mouse DCN (LF-113), and rabbit anti-human fibronectin (Sigma-Aldrich, Deisenhofen, Germany); mouse antibody to phos-photyrosine (clone PY20; Biomol, Hamburg, Germany); rabbit anti-β-chain of the IGF-IR (sc-713) and rabbit anti-β-tubulin (both from Santa Cruz Biotechnologies, Heidelberg, Germany); and anti-phospho-IGF-R (Tyr1131)/insulin receptor (Tyr1146), anti-phospho-PDK1 (Ser241), anti-PDK1, anti-phospho-PKCζ/λ (Thr410/403), anti-PKCζ, anti-phospho-Akt (Thr 308), anti-phospho-Akt (Ser473), anti-Akt, anti-phosphorylated Erk1/2 (Thr202/204), anti-total-Erk1/2, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6 kinase (Thr389), anti-p70 S6 kinase, anti-phospho-S6 ribosomal protein (Ser235/236), anti-S6 ribosomal protein, anti-phospho-eIF4B (Ser422), and anti-eIF4B (all raised in rabbits and all from Cell Signaling Technologies, Frankfurt/Main, Germany). ..

Produced:

Article Title: Evidence of a Cell Surface Role for Hsp90 Complex Proteins Mediating Neuroblast Migration in the Subventricular Zone
Article Snippet: In both groups, medium was replaced, however in the experimental groups, polyclonal antibodies recognizing Hsp90 (1 mg/mL, dilution 150 μg/mL-Milipore) and HOP/STI1 (2.2 μg/μL, Bethyl, rabbit 33, dilution 1:200; 0.011 μg/μL), as well as Hsp90 inhibitor Geldanamycin (100, 10, and 1 nM-Tocris Bioscience, 1368) and VER155008 (100 nM, 10 nM and 1 nM-Tocris Bioscience, 3803) were added to the medium. .. As a control, SVZ explants were treated anti-mouse IgG produced in Rabbit (Sigma-Aldrich A9044). .. SVZ migration

Blocking Assay:

Article Title: Two RSV Platforms for G, F, or G+F Proteins VLPs
Article Snippet: F, Ga, and Gb Antibody EIAs The secreted form of F, Ga, or Gb protein antigens was produced from stably-transfected 293F cells in serum-free media and coated onto a 96-well microtiter plate in coating buffer (15 mM Na2 CO3 , 35 mM NaHCO3 , 3 mM NaN3 , pH 9.4–9.6). .. The plates were incubated in 2% nonfat dry milk dissolved in PBS blocking solution for 2 h at 37 °C, washed with PBS-T (PBS + 0.15% Tween-20), and serum specimens at 1:200 dilution added to the wells, incubated for 1 h at 37 °C, the plates washed with PBS-T, and goat anti mouse IgG-HRP (1:5000) added and incubated for 1 h at 37 °C. .. Color was developed with o -Phenylenediamine dihydrochloride (OPD) (Sigma-Aldrich, St. Louis, MO, USA) substrate and the reaction stopped after 30 min at RT with 4N H2 SO4 .

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  • 99
    Millipore rabbit anti mouse dcn
    Schematic diagram of the <t>DCN-mediated</t> translational regulation of <t>fibrillin-1</t> in NRK cells. Further details are given in Results.
    Rabbit Anti Mouse Dcn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse dcn/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse dcn - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    Millipore anti dcn antibody produced in rabbit
    Changes in the signaling network of colorectal cancer (CRC) liver metastases upon <t>decorin</t> delivery. Liver-specific transfection with the <t>DCN</t> gene inhibited several RTKs with a concomitant decrease in the activity of the MAPK/ERK and Akt/mTOR/p70S6K pathways. Increased AMPK (possibly via LKB1) and p38/MSK/CREB signaling resulted in elevated phospho-p53 levels; stabilization of this key tumor suppressor may result in a cell cycle blockade. Inhibition of STATs and c-Jun proteins also contributes to the antitumor action of decorin. Color code: red: decreased; green: increased; orange: no change; grey: not examined. Additional details are included in the text.
    Anti Dcn Antibody Produced In Rabbit, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti dcn antibody produced in rabbit/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti dcn antibody produced in rabbit - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    94
    Millipore decorin
    The absence of <t>decorin</t> impairs the autophagic response. ( A ) ELISA results from plasma collected from fed and fasted WT mice depicting relative decorin levels. ( B ) Representative immunoblot of the autophagic marker, Sqstm1/p62, demonstrating accumulation
    Decorin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/decorin/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    decorin - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Schematic diagram of the DCN-mediated translational regulation of fibrillin-1 in NRK cells. Further details are given in Results.

    Journal:

    Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin

    doi: 10.2353/ajpath.2007.060497

    Figure Lengend Snippet: Schematic diagram of the DCN-mediated translational regulation of fibrillin-1 in NRK cells. Further details are given in Results.

    Article Snippet: Antibodies used for Western blots were as follows: rabbit anti-human fibrillin-1, rabbit-anti-human DCN, rabbit anti-mouse DCN (LF-113), and rabbit anti-human fibronectin (Sigma-Aldrich, Deisenhofen, Germany); mouse antibody to phos-photyrosine (clone PY20; Biomol, Hamburg, Germany); rabbit anti-β-chain of the IGF-IR (sc-713) and rabbit anti-β-tubulin (both from Santa Cruz Biotechnologies, Heidelberg, Germany); and anti-phospho-IGF-R (Tyr1131)/insulin receptor (Tyr1146), anti-phospho-PDK1 (Ser241), anti-PDK1, anti-phospho-PKCζ/λ (Thr410/403), anti-PKCζ, anti-phospho-Akt (Thr 308), anti-phospho-Akt (Ser473), anti-Akt, anti-phosphorylated Erk1/2 (Thr202/204), anti-total-Erk1/2, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6 kinase (Thr389), anti-p70 S6 kinase, anti-phospho-S6 ribosomal protein (Ser235/236), anti-S6 ribosomal protein, anti-phospho-eIF4B (Ser422), and anti-eIF4B (all raised in rabbits and all from Cell Signaling Technologies, Frankfurt/Main, Germany).

    Techniques:

    Decorin and IGF-I induce fibrillin-1 protein synthesis in normal rat kidney fibroblasts via the IGF-IR. A and B show representative Western blots for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I

    Journal:

    Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin

    doi: 10.2353/ajpath.2007.060497

    Figure Lengend Snippet: Decorin and IGF-I induce fibrillin-1 protein synthesis in normal rat kidney fibroblasts via the IGF-IR. A and B show representative Western blots for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I

    Article Snippet: Antibodies used for Western blots were as follows: rabbit anti-human fibrillin-1, rabbit-anti-human DCN, rabbit anti-mouse DCN (LF-113), and rabbit anti-human fibronectin (Sigma-Aldrich, Deisenhofen, Germany); mouse antibody to phos-photyrosine (clone PY20; Biomol, Hamburg, Germany); rabbit anti-β-chain of the IGF-IR (sc-713) and rabbit anti-β-tubulin (both from Santa Cruz Biotechnologies, Heidelberg, Germany); and anti-phospho-IGF-R (Tyr1131)/insulin receptor (Tyr1146), anti-phospho-PDK1 (Ser241), anti-PDK1, anti-phospho-PKCζ/λ (Thr410/403), anti-PKCζ, anti-phospho-Akt (Thr 308), anti-phospho-Akt (Ser473), anti-Akt, anti-phosphorylated Erk1/2 (Thr202/204), anti-total-Erk1/2, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6 kinase (Thr389), anti-p70 S6 kinase, anti-phospho-S6 ribosomal protein (Ser235/236), anti-S6 ribosomal protein, anti-phospho-eIF4B (Ser422), and anti-eIF4B (all raised in rabbits and all from Cell Signaling Technologies, Frankfurt/Main, Germany).

    Techniques: Western Blot, Incubation, Recombinant

    DCN- and IGF-I-dependent regulation of fibrillin-1 in NRK cells involves mTOR. A- - C: DCN- and IGF-I-mediated phosphorylation of mTOR in NRK cells (8 minutes of incubation) was suppressed by inhibition of IGF-IR ( A ), by an inhibition of PI3K ( B ), and by

    Journal:

    Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin

    doi: 10.2353/ajpath.2007.060497

    Figure Lengend Snippet: DCN- and IGF-I-dependent regulation of fibrillin-1 in NRK cells involves mTOR. A- - C: DCN- and IGF-I-mediated phosphorylation of mTOR in NRK cells (8 minutes of incubation) was suppressed by inhibition of IGF-IR ( A ), by an inhibition of PI3K ( B ), and by

    Article Snippet: Antibodies used for Western blots were as follows: rabbit anti-human fibrillin-1, rabbit-anti-human DCN, rabbit anti-mouse DCN (LF-113), and rabbit anti-human fibronectin (Sigma-Aldrich, Deisenhofen, Germany); mouse antibody to phos-photyrosine (clone PY20; Biomol, Hamburg, Germany); rabbit anti-β-chain of the IGF-IR (sc-713) and rabbit anti-β-tubulin (both from Santa Cruz Biotechnologies, Heidelberg, Germany); and anti-phospho-IGF-R (Tyr1131)/insulin receptor (Tyr1146), anti-phospho-PDK1 (Ser241), anti-PDK1, anti-phospho-PKCζ/λ (Thr410/403), anti-PKCζ, anti-phospho-Akt (Thr 308), anti-phospho-Akt (Ser473), anti-Akt, anti-phosphorylated Erk1/2 (Thr202/204), anti-total-Erk1/2, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6 kinase (Thr389), anti-p70 S6 kinase, anti-phospho-S6 ribosomal protein (Ser235/236), anti-S6 ribosomal protein, anti-phospho-eIF4B (Ser422), and anti-eIF4B (all raised in rabbits and all from Cell Signaling Technologies, Frankfurt/Main, Germany).

    Techniques: Incubation, Inhibition

    Involvement of the PI3K/Akt pathway in DCN- and IGF-I-mediated regulation of fibrillin-1 in NRK cells. A: Western blot for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I in the presence or absence

    Journal:

    Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin

    doi: 10.2353/ajpath.2007.060497

    Figure Lengend Snippet: Involvement of the PI3K/Akt pathway in DCN- and IGF-I-mediated regulation of fibrillin-1 in NRK cells. A: Western blot for fibrillin-1 in culture supernatants from NRK cells incubated for 6 hours with recombinant DCN or IGF-I in the presence or absence

    Article Snippet: Antibodies used for Western blots were as follows: rabbit anti-human fibrillin-1, rabbit-anti-human DCN, rabbit anti-mouse DCN (LF-113), and rabbit anti-human fibronectin (Sigma-Aldrich, Deisenhofen, Germany); mouse antibody to phos-photyrosine (clone PY20; Biomol, Hamburg, Germany); rabbit anti-β-chain of the IGF-IR (sc-713) and rabbit anti-β-tubulin (both from Santa Cruz Biotechnologies, Heidelberg, Germany); and anti-phospho-IGF-R (Tyr1131)/insulin receptor (Tyr1146), anti-phospho-PDK1 (Ser241), anti-PDK1, anti-phospho-PKCζ/λ (Thr410/403), anti-PKCζ, anti-phospho-Akt (Thr 308), anti-phospho-Akt (Ser473), anti-Akt, anti-phosphorylated Erk1/2 (Thr202/204), anti-total-Erk1/2, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6 kinase (Thr389), anti-p70 S6 kinase, anti-phospho-S6 ribosomal protein (Ser235/236), anti-S6 ribosomal protein, anti-phospho-eIF4B (Ser422), and anti-eIF4B (all raised in rabbits and all from Cell Signaling Technologies, Frankfurt/Main, Germany).

    Techniques: Western Blot, Incubation, Recombinant

    Decorin deficiency in STZ-induced diabetes is associated with lower accumulation of fibrillin-1 and overexpression of IGF-IR. A and C: Western blots for fibrillin-1 ( A ), IGF-IR ( C ), and β-tubulin in homogenates of diabetic kidneys from Dcn −/−

    Journal:

    Article Title: Decorin-Mediated Regulation of Fibrillin-1 in the Kidney Involves the Insulin-Like Growth Factor-I Receptor and Mammalian Target of Rapamycin

    doi: 10.2353/ajpath.2007.060497

    Figure Lengend Snippet: Decorin deficiency in STZ-induced diabetes is associated with lower accumulation of fibrillin-1 and overexpression of IGF-IR. A and C: Western blots for fibrillin-1 ( A ), IGF-IR ( C ), and β-tubulin in homogenates of diabetic kidneys from Dcn −/−

    Article Snippet: Antibodies used for Western blots were as follows: rabbit anti-human fibrillin-1, rabbit-anti-human DCN, rabbit anti-mouse DCN (LF-113), and rabbit anti-human fibronectin (Sigma-Aldrich, Deisenhofen, Germany); mouse antibody to phos-photyrosine (clone PY20; Biomol, Hamburg, Germany); rabbit anti-β-chain of the IGF-IR (sc-713) and rabbit anti-β-tubulin (both from Santa Cruz Biotechnologies, Heidelberg, Germany); and anti-phospho-IGF-R (Tyr1131)/insulin receptor (Tyr1146), anti-phospho-PDK1 (Ser241), anti-PDK1, anti-phospho-PKCζ/λ (Thr410/403), anti-PKCζ, anti-phospho-Akt (Thr 308), anti-phospho-Akt (Ser473), anti-Akt, anti-phosphorylated Erk1/2 (Thr202/204), anti-total-Erk1/2, anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-p70 S6 kinase (Thr389), anti-p70 S6 kinase, anti-phospho-S6 ribosomal protein (Ser235/236), anti-S6 ribosomal protein, anti-phospho-eIF4B (Ser422), and anti-eIF4B (all raised in rabbits and all from Cell Signaling Technologies, Frankfurt/Main, Germany).

    Techniques: Over Expression, Western Blot

    Changes in the signaling network of colorectal cancer (CRC) liver metastases upon decorin delivery. Liver-specific transfection with the DCN gene inhibited several RTKs with a concomitant decrease in the activity of the MAPK/ERK and Akt/mTOR/p70S6K pathways. Increased AMPK (possibly via LKB1) and p38/MSK/CREB signaling resulted in elevated phospho-p53 levels; stabilization of this key tumor suppressor may result in a cell cycle blockade. Inhibition of STATs and c-Jun proteins also contributes to the antitumor action of decorin. Color code: red: decreased; green: increased; orange: no change; grey: not examined. Additional details are included in the text.

    Journal: Biomolecules

    Article Title: The Protective Role of Decorin in Hepatic Metastasis of Colorectal Carcinoma

    doi: 10.3390/biom10081199

    Figure Lengend Snippet: Changes in the signaling network of colorectal cancer (CRC) liver metastases upon decorin delivery. Liver-specific transfection with the DCN gene inhibited several RTKs with a concomitant decrease in the activity of the MAPK/ERK and Akt/mTOR/p70S6K pathways. Increased AMPK (possibly via LKB1) and p38/MSK/CREB signaling resulted in elevated phospho-p53 levels; stabilization of this key tumor suppressor may result in a cell cycle blockade. Inhibition of STATs and c-Jun proteins also contributes to the antitumor action of decorin. Color code: red: decreased; green: increased; orange: no change; grey: not examined. Additional details are included in the text.

    Article Snippet: The following primary antibodies were used: Decorin (Sigma-Aldrich, #HPA003315, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling, #4695, 1:1000), phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, #4377, 1:1000), GSK-3α/β (Cell Signaling, #5676, 1:1000), phospho-GSK-3α/β (Cell Signaling, #9331, 1:1000), Akt (pan) (Cell Signaling, #4691, 1:1000), Phospho-Akt (Thr308) (Cell Signaling, #2965, 1:1000), β-Catenin (Cell Signaling, #9562, 1:1000), p27Kip1 (Biosource, #AHZ0458, 1:500), and β-Actin (Sigma-Aldrich, #A2228, 1:5000).

    Techniques: Transfection, Activity Assay, Inhibition

    Representative histological images of hematoxylin and eosin-stained control (pLIVE-0) ( a ), and decorin expressing (pLIVE-DCN) ( b ) liver tissues after inoculation of c38 tumor cells. Bar charts ( c ) represent the ratios of tumor-bearing mice in experimental groups of control and DCN expressing groups. In livers overexpressing decorin, a reduced number of liver metastases was observed in parallel with lower liver mass/body ratio. Scale bar = 1000 µm. * p value

    Journal: Biomolecules

    Article Title: The Protective Role of Decorin in Hepatic Metastasis of Colorectal Carcinoma

    doi: 10.3390/biom10081199

    Figure Lengend Snippet: Representative histological images of hematoxylin and eosin-stained control (pLIVE-0) ( a ), and decorin expressing (pLIVE-DCN) ( b ) liver tissues after inoculation of c38 tumor cells. Bar charts ( c ) represent the ratios of tumor-bearing mice in experimental groups of control and DCN expressing groups. In livers overexpressing decorin, a reduced number of liver metastases was observed in parallel with lower liver mass/body ratio. Scale bar = 1000 µm. * p value

    Article Snippet: The following primary antibodies were used: Decorin (Sigma-Aldrich, #HPA003315, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling, #4695, 1:1000), phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, #4377, 1:1000), GSK-3α/β (Cell Signaling, #5676, 1:1000), phospho-GSK-3α/β (Cell Signaling, #9331, 1:1000), Akt (pan) (Cell Signaling, #4691, 1:1000), Phospho-Akt (Thr308) (Cell Signaling, #2965, 1:1000), β-Catenin (Cell Signaling, #9562, 1:1000), p27Kip1 (Biosource, #AHZ0458, 1:500), and β-Actin (Sigma-Aldrich, #A2228, 1:5000).

    Techniques: Staining, Expressing, Mouse Assay

    Phosphorylation of epidermal growth factor receptor (EGFR), MSPR and PDGFRα receptors in mock-transfected vs. DCN-transfected livers. Overexpression of decorin reduced the levels of active receptor tyrosine kinases (RTKs) in the DCN-transfected group. Data are shown as mean ± SD. * p value

    Journal: Biomolecules

    Article Title: The Protective Role of Decorin in Hepatic Metastasis of Colorectal Carcinoma

    doi: 10.3390/biom10081199

    Figure Lengend Snippet: Phosphorylation of epidermal growth factor receptor (EGFR), MSPR and PDGFRα receptors in mock-transfected vs. DCN-transfected livers. Overexpression of decorin reduced the levels of active receptor tyrosine kinases (RTKs) in the DCN-transfected group. Data are shown as mean ± SD. * p value

    Article Snippet: The following primary antibodies were used: Decorin (Sigma-Aldrich, #HPA003315, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling, #4695, 1:1000), phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, #4377, 1:1000), GSK-3α/β (Cell Signaling, #5676, 1:1000), phospho-GSK-3α/β (Cell Signaling, #9331, 1:1000), Akt (pan) (Cell Signaling, #4691, 1:1000), Phospho-Akt (Thr308) (Cell Signaling, #2965, 1:1000), β-Catenin (Cell Signaling, #9562, 1:1000), p27Kip1 (Biosource, #AHZ0458, 1:500), and β-Actin (Sigma-Aldrich, #A2228, 1:5000).

    Techniques: Transfection, Over Expression

    The absence of decorin impairs the autophagic response. ( A ) ELISA results from plasma collected from fed and fasted WT mice depicting relative decorin levels. ( B ) Representative immunoblot of the autophagic marker, Sqstm1/p62, demonstrating accumulation

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    Article Title: Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy

    doi: 10.1016/j.matbio.2015.09.001

    Figure Lengend Snippet: The absence of decorin impairs the autophagic response. ( A ) ELISA results from plasma collected from fed and fasted WT mice depicting relative decorin levels. ( B ) Representative immunoblot of the autophagic marker, Sqstm1/p62, demonstrating accumulation

    Article Snippet: Antibodies were purchased as follows: Decorin (R & D Biosystems, AF1060), LC3 (Sigma, L7543), Gapdh (Cell Signaling, 14C10), and Actb (Abcam, ab8227).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Marker

    Fasting induces decorin expression in vivo. ( A ) Gene expression analysis via qPCR of fed and fasted WT hearts. ( B ) Analysis of crude protein extracts for decorin proteoglycan and protein core in heart tissue of fed and fasted WT mice. Gapdh was used as

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    Article Title: Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy

    doi: 10.1016/j.matbio.2015.09.001

    Figure Lengend Snippet: Fasting induces decorin expression in vivo. ( A ) Gene expression analysis via qPCR of fed and fasted WT hearts. ( B ) Analysis of crude protein extracts for decorin proteoglycan and protein core in heart tissue of fed and fasted WT mice. Gapdh was used as

    Article Snippet: Antibodies were purchased as follows: Decorin (R & D Biosystems, AF1060), LC3 (Sigma, L7543), Gapdh (Cell Signaling, 14C10), and Actb (Abcam, ab8227).

    Techniques: Expressing, In Vivo, Real-time Polymerase Chain Reaction, Mouse Assay

    Formation of GFP-LC3 puncta in response to fasting is diminished in GFP-LC3; Dcn−/− hearts. ( A-D ) Representative cryo-sections of GFP-LC3; Dcn+/+ hearts demonstrating the increase in decorin immunoreactivity in the fasted GFP-LC3; Dcn+/+

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    Article Title: Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy

    doi: 10.1016/j.matbio.2015.09.001

    Figure Lengend Snippet: Formation of GFP-LC3 puncta in response to fasting is diminished in GFP-LC3; Dcn−/− hearts. ( A-D ) Representative cryo-sections of GFP-LC3; Dcn+/+ hearts demonstrating the increase in decorin immunoreactivity in the fasted GFP-LC3; Dcn+/+

    Article Snippet: Antibodies were purchased as follows: Decorin (R & D Biosystems, AF1060), LC3 (Sigma, L7543), Gapdh (Cell Signaling, 14C10), and Actb (Abcam, ab8227).

    Techniques: