rabbit anti mers cov antibodies  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    MERS CoV Spike Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant recombinant MERS CoV NCoV Novel coronavirus Spike Protein Catalog 40069 V08B AFS88936 1 Met1 Trp1297
    Catalog Number:
    40069-R723
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    MERS CoV
    Applications:
    Microneutralizaiton(MN)
    Immunogen:
    Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297) (Catalog#40069-V08B)
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-RBD Antibody, Anti-S Antibody, Anti-s1 Antibody, Anti-Spike RBD Antibody
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Sino Biological rabbit anti mers cov antibodies
    Characterization of <t>anti-MERS-S2P</t> IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their <t>MERS-CoV</t> specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    This antibody was obtained from a rabbit immunized with purified recombinant recombinant MERS CoV NCoV Novel coronavirus Spike Protein Catalog 40069 V08B AFS88936 1 Met1 Trp1297
    https://www.bioz.com/result/rabbit anti mers cov antibodies/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mers cov antibodies - by Bioz Stars, 2021-05
    96/100 stars

    Images

    1) Product Images from "Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning"

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    Journal: Antibodies

    doi: 10.3390/antib8030042

    Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    Figure Legend Snippet: Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Techniques Used: Immunofluorescence, Infection, Size-exclusion Chromatography, SPR Assay, Chromatin Immunoprecipitation, Binding Assay

    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.
    Figure Legend Snippet: Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Techniques Used: Clone Assay, Enzyme-linked Immunosorbent Assay

    Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.
    Figure Legend Snippet: Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Sandwich ELISA

    Related Articles

    Positive Control:

    Article Title: Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial
    Article Snippet: Two-fold diluted serum was incubated with 2 × 106 viral particles of pseudotyped virus for 60 min at 37°C and added to 786-O cells, incubated for 6 h, and replaced with fresh medium; luciferase-reporter activity was measured 3 days later. .. A commercially available neutralising monoclonal antibody (40069-R723, Sino Biological, Beijing, China) was used as a positive control. .. To assess cellular immunity, interferon-γ-linked enzyme-linked immunospot (ELISpot) assays were done with fresh peripheral blood mononuclear cells (PBMCs) to determine responses to the MERS-CoV spike vaccine antigen.

    Infection:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Article Title: Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.
    Article Snippet: Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat.

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Immunofluorescence:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Staining:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Article Title: Diffuse High Intensity PD-L1 Staining in Thymic Epithelial Tumors
    Article Snippet: .. The TMA was stained with rabbit monoclonal antibody (clone 15, Sino Biological) to human PD-L1. .. PD-L1 staining was scored based on intensity as follows: 0=none, 1=equivocal/uninterpretable, 2=weak, and 3=intermediate-strong.

    Incubation:

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning
    Article Snippet: .. The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT. ..

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Article Title: Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.
    Article Snippet: Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat.

    Blocking Assay:

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Luciferase:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Activity Assay:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Derivative Assay:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Sino Biological rabbit anti mers cov antibodies
    Characterization of <t>anti-MERS-S2P</t> IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their <t>MERS-CoV</t> specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    Rabbit Anti Mers Cov Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mers cov antibodies/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mers cov antibodies - by Bioz Stars, 2021-05
    96/100 stars
      Buy from Supplier

    95
    Sino Biological mers cov nucleocapsid protein rabbit antibody
    Single-dose vaccination with ChAdOx1 <t>MERS</t> protects rhesus macaques against bronchointerstitial pneumonia caused by <t>MERS-CoV</t> challenge. Rhesus macaques were vaccinated with a prime-boost or prime-only regimen of ChAdOx1 MERS, or with ChAdOx1 GFP and challenged with MERS-CoV. (A) Ventrodorsal thoracic radiographs collected on 6 DPI. A marker (R) indicates right side of animal. No pathologic changes were observed in animals vaccinated with ChAdOx1 MERS via a prime-boost or prime-only regimen. Animal vaccinated with ChAdOx1 GFP shows focally extensive area of increased pulmonary opacity and deviation of the cardiac silhouette, highlighted in the circle located in the middle and caudal lung lobes. (B) Thoracic radiographs of each animal were scored per lung lobe resulting in a maximum score of 18. Values were averaged per group per day, shown is mean with standard deviation. See Table S2 for more details. (C) Gross pathology of lungs shows no pathologic changes in ChAdOx1 MERS vaccinated animals, and focally extensive areas of consolidation in left cranial, middle and caudal lung lobes in control animals (asterisks). (D) Gross lung lesions were scored for each lung lobe, ventral and dorsal. Values were averaged per group, shown is mean with standard deviation. (E) Lung tissue sections were stained with hematoxylin and eosin. Moderate numbers of lymphocyte accumulation around pulmonary arterioles (asterisks), and mild thickening of alveolar septae by lymphocytes and macrophages (arrow) in lung tissue of animals vaccinated with ChAdOx1 MERS. Marked bronchointerstitial pneumonia with abundant pulmonary edema and fibrin (asterisks), type II pneumocyte hyperplasia (arrow), and increased numbers of alveolar macrophages (arrowhead) in lung tissue of control animals. Magnification = 200x. (F) Lung to body weight ratio was determined for all animals at necropsy. Shown is mean with standard deviation. (G) Lung tissue sections were stained with antibody against MERS-CoV antigen, which is visible as a red-brown staining. No immunoreactivity was found in ChAdOx1 MERS vaccinated animals, whereas multifocal immunoreactivity of type I and II pneumocytes could be found in lung tissue of ChAdOx1 GFP vaccinated animals. (H) Lung tissue sections were scored on severity of lesions (0=no lesions, 1=1-10%, 2=11-25%, 3=26-50%, 4=51-75%, 5=76-100%) and averaged per group. Shown is mean with standard deviation. A = bronchointerstitial pneumonia; B = type II pneumocyte hyperplasia; C = Hemorrhages, edema, fibrin deposits. Statistical significance between groups was determined via two-tailed unpaired Student’s t-test. * = p-value
    Mers Cov Nucleocapsid Protein Rabbit Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov nucleocapsid protein rabbit antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov nucleocapsid protein rabbit antibody - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    94
    Sino Biological polyclonal rabbit anti mers cov nucleocapsid antibodies
    Representative HE findings in respiratory epithelium of the nasal turbinates of <t>MERS-CoV-infected</t> alpacas and immunohistochemical reactions of the commercially available positive controls for comparison. (A) Multifocal infiltration of lamina propria and submucosa by moderate numbers of lymphocytes, macrophages, and single neutrophilic granulocytes (asterisk). (B) Exocytosis of single neutrophilic granulocytes (grey arrow). (C, D) Abundant viral antigen was detected multifocally in the cytoplasm and along the apical membranous region of epithelial cells using a monoclonal mouse (C, Sino Biological Inc.) and <t>polyclonal</t> rabbit anti-MERS-CoV nucleocapsid antibody (D, Sino Biological Inc.) with citrate pretreatment, in a dilution of 1:70 and 1:2,000, respectively. Both antibodies exhibited a similar staining intensity. (A, B) HE staining; 400x, (C, D) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.
    Polyclonal Rabbit Anti Mers Cov Nucleocapsid Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti mers cov nucleocapsid antibodies/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti mers cov nucleocapsid antibodies - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    95
    Sino Biological mers cov s rabbit polyclonal antibodies
    Two Selected G2-escape Mutations and Their Impact on G2 Binding and Neutralization. (A-D) Binding of G2 Fab to immobilized (A) <t>MERS-CoV</t> S1-NTD, (B) MERS-CoV S-2P, (C) MERS-CoV S-2P-S28F and (D) MERS-CoV S-2P-G198D measured by surface plasmon resonance (SPR). The same concentration series of G2 Fab was used in A-D. Best global fit of the data to a 1:1 binding model is shown as colored lines. (E) Neutralization activity of G2 IgG was measured against pseudotyped lentivirus bearing MERS-CoV S (WT) and two variants (S28F and G198D). Percent neutralization of WT (red), S28F (blue) and G198D (black) S pseudovirions at the different antibody concentrations is shown. Data points represent the mean of three technical replicates with standard errors. (F) Neutralization activity of G2 IgG was measured against authentic MERS-CoV (WT) and the G198D variant. Percent neutralization of WT (red) and G198D (black) MERS-CoV at the different antibody concentrations is shown. Data points for the wild-type virus represent the mean of two technical replicates.
    Mers Cov S Rabbit Polyclonal Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov s rabbit polyclonal antibodies/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov s rabbit polyclonal antibodies - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Article Snippet: The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT.

    Techniques: Immunofluorescence, Infection, Size-exclusion Chromatography, SPR Assay, Chromatin Immunoprecipitation, Binding Assay

    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Article Snippet: The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT.

    Techniques: Clone Assay, Enzyme-linked Immunosorbent Assay

    Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Article Snippet: The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT.

    Techniques: Enzyme-linked Immunosorbent Assay, Sandwich ELISA

    Single-dose vaccination with ChAdOx1 MERS protects rhesus macaques against bronchointerstitial pneumonia caused by MERS-CoV challenge. Rhesus macaques were vaccinated with a prime-boost or prime-only regimen of ChAdOx1 MERS, or with ChAdOx1 GFP and challenged with MERS-CoV. (A) Ventrodorsal thoracic radiographs collected on 6 DPI. A marker (R) indicates right side of animal. No pathologic changes were observed in animals vaccinated with ChAdOx1 MERS via a prime-boost or prime-only regimen. Animal vaccinated with ChAdOx1 GFP shows focally extensive area of increased pulmonary opacity and deviation of the cardiac silhouette, highlighted in the circle located in the middle and caudal lung lobes. (B) Thoracic radiographs of each animal were scored per lung lobe resulting in a maximum score of 18. Values were averaged per group per day, shown is mean with standard deviation. See Table S2 for more details. (C) Gross pathology of lungs shows no pathologic changes in ChAdOx1 MERS vaccinated animals, and focally extensive areas of consolidation in left cranial, middle and caudal lung lobes in control animals (asterisks). (D) Gross lung lesions were scored for each lung lobe, ventral and dorsal. Values were averaged per group, shown is mean with standard deviation. (E) Lung tissue sections were stained with hematoxylin and eosin. Moderate numbers of lymphocyte accumulation around pulmonary arterioles (asterisks), and mild thickening of alveolar septae by lymphocytes and macrophages (arrow) in lung tissue of animals vaccinated with ChAdOx1 MERS. Marked bronchointerstitial pneumonia with abundant pulmonary edema and fibrin (asterisks), type II pneumocyte hyperplasia (arrow), and increased numbers of alveolar macrophages (arrowhead) in lung tissue of control animals. Magnification = 200x. (F) Lung to body weight ratio was determined for all animals at necropsy. Shown is mean with standard deviation. (G) Lung tissue sections were stained with antibody against MERS-CoV antigen, which is visible as a red-brown staining. No immunoreactivity was found in ChAdOx1 MERS vaccinated animals, whereas multifocal immunoreactivity of type I and II pneumocytes could be found in lung tissue of ChAdOx1 GFP vaccinated animals. (H) Lung tissue sections were scored on severity of lesions (0=no lesions, 1=1-10%, 2=11-25%, 3=26-50%, 4=51-75%, 5=76-100%) and averaged per group. Shown is mean with standard deviation. A = bronchointerstitial pneumonia; B = type II pneumocyte hyperplasia; C = Hemorrhages, edema, fibrin deposits. Statistical significance between groups was determined via two-tailed unpaired Student’s t-test. * = p-value

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: Single-dose vaccination with ChAdOx1 MERS protects rhesus macaques against bronchointerstitial pneumonia caused by MERS-CoV challenge. Rhesus macaques were vaccinated with a prime-boost or prime-only regimen of ChAdOx1 MERS, or with ChAdOx1 GFP and challenged with MERS-CoV. (A) Ventrodorsal thoracic radiographs collected on 6 DPI. A marker (R) indicates right side of animal. No pathologic changes were observed in animals vaccinated with ChAdOx1 MERS via a prime-boost or prime-only regimen. Animal vaccinated with ChAdOx1 GFP shows focally extensive area of increased pulmonary opacity and deviation of the cardiac silhouette, highlighted in the circle located in the middle and caudal lung lobes. (B) Thoracic radiographs of each animal were scored per lung lobe resulting in a maximum score of 18. Values were averaged per group per day, shown is mean with standard deviation. See Table S2 for more details. (C) Gross pathology of lungs shows no pathologic changes in ChAdOx1 MERS vaccinated animals, and focally extensive areas of consolidation in left cranial, middle and caudal lung lobes in control animals (asterisks). (D) Gross lung lesions were scored for each lung lobe, ventral and dorsal. Values were averaged per group, shown is mean with standard deviation. (E) Lung tissue sections were stained with hematoxylin and eosin. Moderate numbers of lymphocyte accumulation around pulmonary arterioles (asterisks), and mild thickening of alveolar septae by lymphocytes and macrophages (arrow) in lung tissue of animals vaccinated with ChAdOx1 MERS. Marked bronchointerstitial pneumonia with abundant pulmonary edema and fibrin (asterisks), type II pneumocyte hyperplasia (arrow), and increased numbers of alveolar macrophages (arrowhead) in lung tissue of control animals. Magnification = 200x. (F) Lung to body weight ratio was determined for all animals at necropsy. Shown is mean with standard deviation. (G) Lung tissue sections were stained with antibody against MERS-CoV antigen, which is visible as a red-brown staining. No immunoreactivity was found in ChAdOx1 MERS vaccinated animals, whereas multifocal immunoreactivity of type I and II pneumocytes could be found in lung tissue of ChAdOx1 GFP vaccinated animals. (H) Lung tissue sections were scored on severity of lesions (0=no lesions, 1=1-10%, 2=11-25%, 3=26-50%, 4=51-75%, 5=76-100%) and averaged per group. Shown is mean with standard deviation. A = bronchointerstitial pneumonia; B = type II pneumocyte hyperplasia; C = Hemorrhages, edema, fibrin deposits. Statistical significance between groups was determined via two-tailed unpaired Student’s t-test. * = p-value

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Marker, Standard Deviation, Staining, Two Tailed Test

    ChAdOx1 MERS provides cross-protection against different MERS-CoV strains in the mouse model. (A) Survival curves of ChAdOx1 MERS vaccinated (solid line) and ChAdOx1 GFP vaccinated (dashed line) hDPP4 mice challenged with MERS-CoV. (B) Infectious virus titers in lung tissue collected on 3 DPI from hDPP4 mice challenged with MERS-CoV. Shown is mean titer with standard deviation.

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: ChAdOx1 MERS provides cross-protection against different MERS-CoV strains in the mouse model. (A) Survival curves of ChAdOx1 MERS vaccinated (solid line) and ChAdOx1 GFP vaccinated (dashed line) hDPP4 mice challenged with MERS-CoV. (B) Infectious virus titers in lung tissue collected on 3 DPI from hDPP4 mice challenged with MERS-CoV. Shown is mean titer with standard deviation.

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Mouse Assay, Standard Deviation

    Maximum likelihood phylogeny of all published full-length MERS-CoV nucleotide sequences. Strains highlighted in green: human isolates used in this study; strains highlighted in blue: camel isolates used in this study; strain highlighted in red: ChAdOx1 MERS strain.

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: Maximum likelihood phylogeny of all published full-length MERS-CoV nucleotide sequences. Strains highlighted in green: human isolates used in this study; strains highlighted in blue: camel isolates used in this study; strain highlighted in red: ChAdOx1 MERS strain.

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques:

    Vaccination of rhesus macaques with ChAdOx1 MERS elicits a humoral immune response. Serum samples were collected from non-human primates at times of vaccination (−56 DPI and -28 DPI), 14 days later and at challenge. (A) Overview of experimental timeline. V-M = vaccination with ChAdOx1 MERS; V-G = vaccination with ChAdOx1 GFP; E = exam; C = challenge and exam; N = exam and necropsy (B) Two-fold serial-diluted serum samples were tested for MERS-CoV S-specific antibodies using ELISA. (C) ELISA-positive two-fold serial-diluted serum samples were tested for neutralizing antibodies against MERS-CoV in VeroE6 cells. Line = geometric mean, dotted line = limit of detection. Statistical significance between -28 DPI and -14 DPI in the prime-boost group was determined via one-tailed paired Student’s t-test. ** = p-value

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: Vaccination of rhesus macaques with ChAdOx1 MERS elicits a humoral immune response. Serum samples were collected from non-human primates at times of vaccination (−56 DPI and -28 DPI), 14 days later and at challenge. (A) Overview of experimental timeline. V-M = vaccination with ChAdOx1 MERS; V-G = vaccination with ChAdOx1 GFP; E = exam; C = challenge and exam; N = exam and necropsy (B) Two-fold serial-diluted serum samples were tested for MERS-CoV S-specific antibodies using ELISA. (C) ELISA-positive two-fold serial-diluted serum samples were tested for neutralizing antibodies against MERS-CoV in VeroE6 cells. Line = geometric mean, dotted line = limit of detection. Statistical significance between -28 DPI and -14 DPI in the prime-boost group was determined via one-tailed paired Student’s t-test. ** = p-value

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Enzyme-linked Immunosorbent Assay, One-tailed Test

    Representative HE findings in respiratory epithelium of the nasal turbinates of MERS-CoV-infected alpacas and immunohistochemical reactions of the commercially available positive controls for comparison. (A) Multifocal infiltration of lamina propria and submucosa by moderate numbers of lymphocytes, macrophages, and single neutrophilic granulocytes (asterisk). (B) Exocytosis of single neutrophilic granulocytes (grey arrow). (C, D) Abundant viral antigen was detected multifocally in the cytoplasm and along the apical membranous region of epithelial cells using a monoclonal mouse (C, Sino Biological Inc.) and polyclonal rabbit anti-MERS-CoV nucleocapsid antibody (D, Sino Biological Inc.) with citrate pretreatment, in a dilution of 1:70 and 1:2,000, respectively. Both antibodies exhibited a similar staining intensity. (A, B) HE staining; 400x, (C, D) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.

    Journal: Veterinary Immunology and Immunopathology

    Article Title: Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein

    doi: 10.1016/j.vetimm.2019.109939

    Figure Lengend Snippet: Representative HE findings in respiratory epithelium of the nasal turbinates of MERS-CoV-infected alpacas and immunohistochemical reactions of the commercially available positive controls for comparison. (A) Multifocal infiltration of lamina propria and submucosa by moderate numbers of lymphocytes, macrophages, and single neutrophilic granulocytes (asterisk). (B) Exocytosis of single neutrophilic granulocytes (grey arrow). (C, D) Abundant viral antigen was detected multifocally in the cytoplasm and along the apical membranous region of epithelial cells using a monoclonal mouse (C, Sino Biological Inc.) and polyclonal rabbit anti-MERS-CoV nucleocapsid antibody (D, Sino Biological Inc.) with citrate pretreatment, in a dilution of 1:70 and 1:2,000, respectively. Both antibodies exhibited a similar staining intensity. (A, B) HE staining; 400x, (C, D) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.

    Article Snippet: For positive control and elucidation of virus distribution, additional sections were stained with two different commercially available, previously published monoclonal mouse and polyclonal rabbit anti-MERS-CoV nucleocapsid antibodies (Sino Biological Inc.; ; ), respectively.

    Techniques: Infection, Immunohistochemistry, Staining, Avidin-Biotin Assay

    Representative positive immunohistochemical reactions for human monoclonal antibodies. (A) Antibody 1.2g5 with citrate pretreatment exhibited a strong multifocal MERS-CoV antigen specific signal in the cytoplasm of ciliated respiratory epithelial cells (arrow) and segmentally along the apical membranous region (arrow heads). (B) The same protocol without pretreatment revealed a much fainter but similarly distributed staining of cytoplasm (arrow) and ciliary base (arrow heads). (C) Antibody 1.10f3 with citrate pretreatment displayed a strong diffuse background staining and only few positively stained cilia (arrowhead). Single intraepithelial plasma cells displayed a strong false positive intracytoplasmic staining (white arrow). (D) Antibody 1.6c7 with citrate pretreatment exhibited also a strong diffuse background staining and only few positive staining cilia (arrow heads). (A–D) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.

    Journal: Veterinary Immunology and Immunopathology

    Article Title: Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein

    doi: 10.1016/j.vetimm.2019.109939

    Figure Lengend Snippet: Representative positive immunohistochemical reactions for human monoclonal antibodies. (A) Antibody 1.2g5 with citrate pretreatment exhibited a strong multifocal MERS-CoV antigen specific signal in the cytoplasm of ciliated respiratory epithelial cells (arrow) and segmentally along the apical membranous region (arrow heads). (B) The same protocol without pretreatment revealed a much fainter but similarly distributed staining of cytoplasm (arrow) and ciliary base (arrow heads). (C) Antibody 1.10f3 with citrate pretreatment displayed a strong diffuse background staining and only few positively stained cilia (arrowhead). Single intraepithelial plasma cells displayed a strong false positive intracytoplasmic staining (white arrow). (D) Antibody 1.6c7 with citrate pretreatment exhibited also a strong diffuse background staining and only few positive staining cilia (arrow heads). (A–D) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.

    Article Snippet: For positive control and elucidation of virus distribution, additional sections were stained with two different commercially available, previously published monoclonal mouse and polyclonal rabbit anti-MERS-CoV nucleocapsid antibodies (Sino Biological Inc.; ; ), respectively.

    Techniques: Immunohistochemistry, Staining, Avidin-Biotin Assay

    Representative negative immunohistochemical reactions for human monoclonal antibodies. (A–E) Specific staining for MERS-CoV antigen was absent for the antibodies 1.6f9 (A), 4.6e10 (B), 7.7g6 (C), 1.8e5 (D), and 3.5g6 (E), respectively. Reactions were accompanied by mild to severe non-specific, intracytoplasmic background staining. (A–E) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.

    Journal: Veterinary Immunology and Immunopathology

    Article Title: Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein

    doi: 10.1016/j.vetimm.2019.109939

    Figure Lengend Snippet: Representative negative immunohistochemical reactions for human monoclonal antibodies. (A–E) Specific staining for MERS-CoV antigen was absent for the antibodies 1.6f9 (A), 4.6e10 (B), 7.7g6 (C), 1.8e5 (D), and 3.5g6 (E), respectively. Reactions were accompanied by mild to severe non-specific, intracytoplasmic background staining. (A–E) Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen; 400x.

    Article Snippet: For positive control and elucidation of virus distribution, additional sections were stained with two different commercially available, previously published monoclonal mouse and polyclonal rabbit anti-MERS-CoV nucleocapsid antibodies (Sino Biological Inc.; ; ), respectively.

    Techniques: Immunohistochemistry, Staining, Avidin-Biotin Assay

    Two Selected G2-escape Mutations and Their Impact on G2 Binding and Neutralization. (A-D) Binding of G2 Fab to immobilized (A) MERS-CoV S1-NTD, (B) MERS-CoV S-2P, (C) MERS-CoV S-2P-S28F and (D) MERS-CoV S-2P-G198D measured by surface plasmon resonance (SPR). The same concentration series of G2 Fab was used in A-D. Best global fit of the data to a 1:1 binding model is shown as colored lines. (E) Neutralization activity of G2 IgG was measured against pseudotyped lentivirus bearing MERS-CoV S (WT) and two variants (S28F and G198D). Percent neutralization of WT (red), S28F (blue) and G198D (black) S pseudovirions at the different antibody concentrations is shown. Data points represent the mean of three technical replicates with standard errors. (F) Neutralization activity of G2 IgG was measured against authentic MERS-CoV (WT) and the G198D variant. Percent neutralization of WT (red) and G198D (black) MERS-CoV at the different antibody concentrations is shown. Data points for the wild-type virus represent the mean of two technical replicates.

    Journal: Cell reports

    Article Title: Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD

    doi: 10.1016/j.celrep.2019.08.052

    Figure Lengend Snippet: Two Selected G2-escape Mutations and Their Impact on G2 Binding and Neutralization. (A-D) Binding of G2 Fab to immobilized (A) MERS-CoV S1-NTD, (B) MERS-CoV S-2P, (C) MERS-CoV S-2P-S28F and (D) MERS-CoV S-2P-G198D measured by surface plasmon resonance (SPR). The same concentration series of G2 Fab was used in A-D. Best global fit of the data to a 1:1 binding model is shown as colored lines. (E) Neutralization activity of G2 IgG was measured against pseudotyped lentivirus bearing MERS-CoV S (WT) and two variants (S28F and G198D). Percent neutralization of WT (red), S28F (blue) and G198D (black) S pseudovirions at the different antibody concentrations is shown. Data points represent the mean of three technical replicates with standard errors. (F) Neutralization activity of G2 IgG was measured against authentic MERS-CoV (WT) and the G198D variant. Percent neutralization of WT (red) and G198D (black) MERS-CoV at the different antibody concentrations is shown. Data points for the wild-type virus represent the mean of two technical replicates.

    Article Snippet: Subsequently, cells were stained with MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) and then secondary goat anti-rabbit IgG H & L labeled with Alexa Fluor® 488 (AF488) was added.

    Techniques: Binding Assay, Neutralization, SPR Assay, Concentration Assay, Activity Assay, Variant Assay

    G2 IgG Prevents the Binding of MERS-CoV S Protein to DDP4-Expressing Cells. Normalized binding efficiency of GFP-tagged MERS-CoV S-2P proteins to DPP4-expressing FreeStyle 293F cells in the presence or absence of IgGs was calculated from median fluorescence intensity (MFI) values. FreeStyle 293-F cells were transfected with a plasmid encoding full-length DPP4 60 h before the experiment. Non-transfected cells (NT) incubated with MERS-CoV S-2P, as well as transfected cells incubated with PBS, were used as negative controls. AM14 is an irrelevant RSV F-specific neutralizing antibody used as another negative control. Bar graph shows the mean and error bars indicate the standard deviation (n = 3 biologically independent experiments with two technical replicates).

    Journal: Cell reports

    Article Title: Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD

    doi: 10.1016/j.celrep.2019.08.052

    Figure Lengend Snippet: G2 IgG Prevents the Binding of MERS-CoV S Protein to DDP4-Expressing Cells. Normalized binding efficiency of GFP-tagged MERS-CoV S-2P proteins to DPP4-expressing FreeStyle 293F cells in the presence or absence of IgGs was calculated from median fluorescence intensity (MFI) values. FreeStyle 293-F cells were transfected with a plasmid encoding full-length DPP4 60 h before the experiment. Non-transfected cells (NT) incubated with MERS-CoV S-2P, as well as transfected cells incubated with PBS, were used as negative controls. AM14 is an irrelevant RSV F-specific neutralizing antibody used as another negative control. Bar graph shows the mean and error bars indicate the standard deviation (n = 3 biologically independent experiments with two technical replicates).

    Article Snippet: Subsequently, cells were stained with MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) and then secondary goat anti-rabbit IgG H & L labeled with Alexa Fluor® 488 (AF488) was added.

    Techniques: Binding Assay, Expressing, Fluorescence, Transfection, Plasmid Preparation, Incubation, Negative Control, Standard Deviation

    G2-mediated Inhibition of DPP4 binding to MERS-CoV S Depends on Oligomeric State of the Spike (A–C) Normalized binding efficiency of DPP4, in the presence or absence of Fab, to cells transfected with plasmids encoding (A) membrane-anchored S1 (S1-TM), (B) full-length S-WT (S-WT-FL) and (C) full-length S-2P (S-2P-FL). Cells incubated with PBS were used as negative controls. Bar graph shows the mean and error bars indicate the standard deviation (n = 3 biologically independent experiments with two technical replicates). (D) Surface plasmon resonance competition assay. Response curves for MERS-CoV S-2P, alone or in the presence of a 5-fold molar excess of indicated Fabs or IgGs, passed over immobilized DPP4 ectodomain. The curves for S-2P or S-2P supplemented with Fab or IgG are shown with a solid line, whereas control curves for samples without S-2P are shown with a dotted line.

    Journal: Cell reports

    Article Title: Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD

    doi: 10.1016/j.celrep.2019.08.052

    Figure Lengend Snippet: G2-mediated Inhibition of DPP4 binding to MERS-CoV S Depends on Oligomeric State of the Spike (A–C) Normalized binding efficiency of DPP4, in the presence or absence of Fab, to cells transfected with plasmids encoding (A) membrane-anchored S1 (S1-TM), (B) full-length S-WT (S-WT-FL) and (C) full-length S-2P (S-2P-FL). Cells incubated with PBS were used as negative controls. Bar graph shows the mean and error bars indicate the standard deviation (n = 3 biologically independent experiments with two technical replicates). (D) Surface plasmon resonance competition assay. Response curves for MERS-CoV S-2P, alone or in the presence of a 5-fold molar excess of indicated Fabs or IgGs, passed over immobilized DPP4 ectodomain. The curves for S-2P or S-2P supplemented with Fab or IgG are shown with a solid line, whereas control curves for samples without S-2P are shown with a dotted line.

    Article Snippet: Subsequently, cells were stained with MERS-CoV S rabbit polyclonal antibodies (Sino Biological, Beijing, China) and then secondary goat anti-rabbit IgG H & L labeled with Alexa Fluor® 488 (AF488) was added.

    Techniques: Inhibition, Binding Assay, Transfection, Incubation, Standard Deviation, SPR Assay, Competitive Binding Assay