rabbit anti melanopsin  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore rabbit anti melanopsin
    Interactions between dendrites of secretoneurin-IR amacrine cells (red) and <t>melanopsin-IR</t> (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.
    Rabbit Anti Melanopsin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti melanopsin/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti melanopsin - by Bioz Stars, 2020-08
    90/100 stars

    Images

    1) Product Images from "Wavy Multistratified Amacrine Cells in the Monkey Retina Contain Immunoreactive Secretoneurin"

    Article Title: Wavy Multistratified Amacrine Cells in the Monkey Retina Contain Immunoreactive Secretoneurin

    Journal: Peptides

    doi: 10.1016/j.peptides.2017.06.005

    Interactions between dendrites of secretoneurin-IR amacrine cells (red) and melanopsin-IR (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.
    Figure Legend Snippet: Interactions between dendrites of secretoneurin-IR amacrine cells (red) and melanopsin-IR (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.

    Techniques Used:

    2) Product Images from "Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development"

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development

    Journal: Developmental neurobiology

    doi: 10.1002/dneu.22260

    Example of a close apposition between a complex outer retinal dendrite of a melanopsin ganglion cells and a cone axon terminal at P12. Epifluorescence photomicrographs of a vertical retina section. A. VGlut1 immunohistochemistry (green) labels photoreceptor
    Figure Legend Snippet: Example of a close apposition between a complex outer retinal dendrite of a melanopsin ganglion cells and a cone axon terminal at P12. Epifluorescence photomicrographs of a vertical retina section. A. VGlut1 immunohistochemistry (green) labels photoreceptor

    Techniques Used: Immunohistochemistry

    Melanopsin ganglion cells apparently do not modulate starburst amacrine cell wave bursts. A. Whole cell patch recordings of ON starburst amacrine cells located in the ganglion cell layer of isolated whole-mounted retinas from early postnatal mice (P4
    Figure Legend Snippet: Melanopsin ganglion cells apparently do not modulate starburst amacrine cell wave bursts. A. Whole cell patch recordings of ON starburst amacrine cells located in the ganglion cell layer of isolated whole-mounted retinas from early postnatal mice (P4

    Techniques Used: Isolation, Mouse Assay

    Morphology of outer retinal dendrites of melanopsin immunopositive melanopsin ganglion cells at various developmental stages. A, B: Schematic diagrams of simple (A) and complex (B) outer retinal dendrites ascending from the main dendritic arbor of an
    Figure Legend Snippet: Morphology of outer retinal dendrites of melanopsin immunopositive melanopsin ganglion cells at various developmental stages. A, B: Schematic diagrams of simple (A) and complex (B) outer retinal dendrites ascending from the main dendritic arbor of an

    Techniques Used:

    Complex outer retinal dendrites (CORDs) of melanopsin ganglion cells in relation to cone pedicles as seen in retinal wholemounts from P8 mice. All images reconstructed from z-stacks of deconvolved confocal sections. Panels A–I show one such dendrite,
    Figure Legend Snippet: Complex outer retinal dendrites (CORDs) of melanopsin ganglion cells in relation to cone pedicles as seen in retinal wholemounts from P8 mice. All images reconstructed from z-stacks of deconvolved confocal sections. Panels A–I show one such dendrite,

    Techniques Used: Mouse Assay

    Development of dendritic stratification of melanopsin ganglion cells and starburst amacrine cells in the early postnatal period in mice. Epifluorescence photomicrographs of vertical retina sections in mice from three ages: P4 (A), P8 (B) and P10 (C).
    Figure Legend Snippet: Development of dendritic stratification of melanopsin ganglion cells and starburst amacrine cells in the early postnatal period in mice. Epifluorescence photomicrographs of vertical retina sections in mice from three ages: P4 (A), P8 (B) and P10 (C).

    Techniques Used: Mouse Assay

    3) Product Images from "CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4"

    Article Title: CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1008554

    The isoform γC4 is necessary for normal neuronal survival. Cryosections from P0 spinal cords were immunostained for the indicated markers (green; DAPI counterstain for nuclei, blue) A-B ) Immunostaing for NeuN revealed grossly smaller spinal cords and neuronal loss in C4KO mutants, but not 1R1 animals. C-D ) This was accompanied by reactive gliosis in C4KO. E-H ) Interneuron subtypes were analyzed as in Fig 4 ( E-F , FoxP2 and G-H , Pax2). K-L ) Ventral interneurons were drastically reduced in C4KO mutants; decreases in 1R1 mutants were statistically significant, but substantially more modest. I-J ) Apoptosis was increased in C4KO animals, but not in 1R1 mutants, as demonstrated by CC3 immunolabeling (quantified in M ). N-Q ) Whole mount retinas from 1R1 mutants were assayed for neuronal loss at 2 weeks of age and in adult as in Fig 6 . There were no reductions in ( N ) VGLUT3+ amacrine cells, ( O ) TH+ dopaminergic amacrine cells, or ( P ) Melanopsin+ ipRGCs. Q ) At 2 weeks of age, Brn3b+ RGCs were also not reduced, but their density was significantly lower in adult retinas. R ) Summary of phenotypes in the analyzed strains. Mutations that disrupted Pcdhgc4 resulted in high levels of cell death (+++) and postnatal lethality, while mutations that spared Pcdhgc4 resulted in little to no cell death and animal survival. Scale bar is 200 μm. * = p
    Figure Legend Snippet: The isoform γC4 is necessary for normal neuronal survival. Cryosections from P0 spinal cords were immunostained for the indicated markers (green; DAPI counterstain for nuclei, blue) A-B ) Immunostaing for NeuN revealed grossly smaller spinal cords and neuronal loss in C4KO mutants, but not 1R1 animals. C-D ) This was accompanied by reactive gliosis in C4KO. E-H ) Interneuron subtypes were analyzed as in Fig 4 ( E-F , FoxP2 and G-H , Pax2). K-L ) Ventral interneurons were drastically reduced in C4KO mutants; decreases in 1R1 mutants were statistically significant, but substantially more modest. I-J ) Apoptosis was increased in C4KO animals, but not in 1R1 mutants, as demonstrated by CC3 immunolabeling (quantified in M ). N-Q ) Whole mount retinas from 1R1 mutants were assayed for neuronal loss at 2 weeks of age and in adult as in Fig 6 . There were no reductions in ( N ) VGLUT3+ amacrine cells, ( O ) TH+ dopaminergic amacrine cells, or ( P ) Melanopsin+ ipRGCs. Q ) At 2 weeks of age, Brn3b+ RGCs were also not reduced, but their density was significantly lower in adult retinas. R ) Summary of phenotypes in the analyzed strains. Mutations that disrupted Pcdhgc4 resulted in high levels of cell death (+++) and postnatal lethality, while mutations that spared Pcdhgc4 resulted in little to no cell death and animal survival. Scale bar is 200 μm. * = p

    Techniques Used: Immunolabeling

    Reduced retinal neuron numbers in 13R1 mutants. Whole mount retinas from two-week-old mice were immunostained to label neuronal subtypes, imaged en face by confocal microscopy, and cell densities quantified. Analyzed cell types include ( A-E ) VGLUT3+ amacrine cells, ( F-J ) TH+ dopaminergic amacrine cells, ( K-O ) Melanopsin+ ipRGCs, and ( P-T ) Brn3a+ RGCs. For all four of the subtypes assayed, densities were significantly reduced in 13R1/cRKO retinas compared to wild-type. Densities were not reduced in either 3R1 or 3R2. Scale bar in D is 100 μm (for A-D ); scale bar in S is 300 μm (for F-S ). ** = p
    Figure Legend Snippet: Reduced retinal neuron numbers in 13R1 mutants. Whole mount retinas from two-week-old mice were immunostained to label neuronal subtypes, imaged en face by confocal microscopy, and cell densities quantified. Analyzed cell types include ( A-E ) VGLUT3+ amacrine cells, ( F-J ) TH+ dopaminergic amacrine cells, ( K-O ) Melanopsin+ ipRGCs, and ( P-T ) Brn3a+ RGCs. For all four of the subtypes assayed, densities were significantly reduced in 13R1/cRKO retinas compared to wild-type. Densities were not reduced in either 3R1 or 3R2. Scale bar in D is 100 μm (for A-D ); scale bar in S is 300 μm (for F-S ). ** = p

    Techniques Used: Mouse Assay, Confocal Microscopy

    Substantial loss of retinal thickness without layer disorganization in 13R1 mutants. A-C ) Cryosections taken in cross section through retinas of ( A ) wild type, ( B ) 13R1/cRKO, and ( C ) 3R2 mutants at two weeks of age were immunostained for SV2 to label the synaptic layers (outer and inner plexiform layers; OPL, IPL) and DAPI to label the inner and outer nuclear layers (ONL, INL) and retinal ganglion cell layer (RGL). The IPL and INL were both notably thinner in 13R1/cRKO than in wild type or 3R2 mutants. This was not accompanied by disorganization of neuronal subtype stratification within the IPL, as revealed by ( D-F ) Melanopsin and TH immunolabeling of ipRGCs and dopaminergic amacrine cells, respectively, and ( G-I ) VGLUT3 and ChAT immunolabeling of glutamatergic amacrine cells and starburst amacrine cells, respectively. Scale bar is 50 μm. Images are representative of at least 6 retinas per genotype analyzed.
    Figure Legend Snippet: Substantial loss of retinal thickness without layer disorganization in 13R1 mutants. A-C ) Cryosections taken in cross section through retinas of ( A ) wild type, ( B ) 13R1/cRKO, and ( C ) 3R2 mutants at two weeks of age were immunostained for SV2 to label the synaptic layers (outer and inner plexiform layers; OPL, IPL) and DAPI to label the inner and outer nuclear layers (ONL, INL) and retinal ganglion cell layer (RGL). The IPL and INL were both notably thinner in 13R1/cRKO than in wild type or 3R2 mutants. This was not accompanied by disorganization of neuronal subtype stratification within the IPL, as revealed by ( D-F ) Melanopsin and TH immunolabeling of ipRGCs and dopaminergic amacrine cells, respectively, and ( G-I ) VGLUT3 and ChAT immunolabeling of glutamatergic amacrine cells and starburst amacrine cells, respectively. Scale bar is 50 μm. Images are representative of at least 6 retinas per genotype analyzed.

    Techniques Used: Immunolabeling

    4) Product Images from "Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers"

    Article Title: Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers

    Journal: Molecular Vision

    doi:

    Pou4f2 Cre -mediated recombination within the retina. Cre-mediated recombination within the retina was assayed in Pou4f2 Cre/+ Ai9 mice by immunostaining retinal cryosections with a panel of markers. Channels are split out for better visualization. Markers are described in further detail in Table 1 . Abbreviations: mela = melanopsin, Calb = calbindin. Scale bars = 50 μm. Insets = 16 μm boxes.
    Figure Legend Snippet: Pou4f2 Cre -mediated recombination within the retina. Cre-mediated recombination within the retina was assayed in Pou4f2 Cre/+ Ai9 mice by immunostaining retinal cryosections with a panel of markers. Channels are split out for better visualization. Markers are described in further detail in Table 1 . Abbreviations: mela = melanopsin, Calb = calbindin. Scale bars = 50 μm. Insets = 16 μm boxes.

    Techniques Used: Mouse Assay, Immunostaining

    Related Articles

    Incubation:

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development
    Article Snippet: .. Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200). .. Tissue was then washed (6 × 10 min in PBS) and incubated overnight in the secondary antibody for the ChAT antibody (donkey anti-goat Alexa 594; Invitrogen #A-11058; 1:200).

    other:

    Article Title: Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers
    Article Snippet: Antibodies The following antibodies were used in this study: mouse anti-POU4F1/BRN3A (Millipore, Darmstadt, Germany; MAB1585; 1:100); goat anti-POU4F2/BRN3B (Santa Cruz Biotechnology, Santa Cruz, CA; sc-6026; 1:100); rabbit anti-melanopsin (generous gift from Ignacio Provencio; 1:10,000); rabbit anti-tyrosine hydroxylase (Millipore; AB152; 1:500); mouse anti-AP2α (Developmental Studies Hybridoma Bank, Trevor J. Williams; 3B5; 1:50); rabbit anti-calbindin (Swant, Marly, Switzerland; CB-38a; 1:1,000); goat anti-choline acetyltansferase (Millipore; AB144P; 1:250); rabbit anti-bNOS (Sigma, St. Louis, MO; N7280; 1:5,000); mouse anti-PKCα (Santa Cruz Biotechnology, sc-17769; 1:500); rabbit anti-cone arrestin (Millipore; AB15282; 1:10,000); mouse anti-glutamine synthetase (Millipore; MAB302; 1:1,000); mouse anti-calsenilin (Millipore; 05–756; 1:1,000); mouse anti-PAX6 (developed by Kawakami, Developmental Studies Hybridoma; 1:500); and rabbit anti-Cre (Millipore; 69,050-3; 1:500).

    Blocking Assay:

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development
    Article Snippet: .. Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200). .. Tissue was then washed (6 × 10 min in PBS) and incubated overnight in the secondary antibody for the ChAT antibody (donkey anti-goat Alexa 594; Invitrogen #A-11058; 1:200).

    Immunostaining:

    Article Title: Cadherin-6 Mediates Axon-Target Matching in a Non-Image-Forming Visual Circuit
    Article Snippet: .. Immunostaining for GFP was carried out as described in using rabbit anti-GFP (Invitrogen; 1:1000), rabbit anti-melanopsin (ATS; 1:2000), goat anti-ChAT (Chemicon; 1:100), rabbit anti-Cdh6 (Dr. Gregory Dressler University of Michigan; 1:500) or mouse anti-parvalbumin (Chemicon; 1:2000) and Alexa-488, -594, or -647 secondary antibodies. .. Briefly, a 3 ml volume of 0.5% cholera-toxin beta (diluted in sterile saline) conjugated to Alexa-594 (Invitrogen) was injected into the right and left vitreous of Cdh3-GFP mice using a Hamilton syringe with 33 gauge needle.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Millipore rabbit anti melanopsin
    Interactions between dendrites of secretoneurin-IR amacrine cells (red) and <t>melanopsin-IR</t> (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.
    Rabbit Anti Melanopsin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti melanopsin/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti melanopsin - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Interactions between dendrites of secretoneurin-IR amacrine cells (red) and melanopsin-IR (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.

    Journal: Peptides

    Article Title: Wavy Multistratified Amacrine Cells in the Monkey Retina Contain Immunoreactive Secretoneurin

    doi: 10.1016/j.peptides.2017.06.005

    Figure Lengend Snippet: Interactions between dendrites of secretoneurin-IR amacrine cells (red) and melanopsin-IR (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.

    Article Snippet: The first step was 11 days with 1:10,000 rabbit anti-melanopsin, 20% ChemiBLOCKER (Millipore) and 0.3% Triton, and the second was 14 days with 1:10,000 goat anti-secretoneurin in the same diluent.

    Techniques:

    Example of a close apposition between a complex outer retinal dendrite of a melanopsin ganglion cells and a cone axon terminal at P12. Epifluorescence photomicrographs of a vertical retina section. A. VGlut1 immunohistochemistry (green) labels photoreceptor

    Journal: Developmental neurobiology

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development

    doi: 10.1002/dneu.22260

    Figure Lengend Snippet: Example of a close apposition between a complex outer retinal dendrite of a melanopsin ganglion cells and a cone axon terminal at P12. Epifluorescence photomicrographs of a vertical retina section. A. VGlut1 immunohistochemistry (green) labels photoreceptor

    Article Snippet: Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200).

    Techniques: Immunohistochemistry

    Melanopsin ganglion cells apparently do not modulate starburst amacrine cell wave bursts. A. Whole cell patch recordings of ON starburst amacrine cells located in the ganglion cell layer of isolated whole-mounted retinas from early postnatal mice (P4

    Journal: Developmental neurobiology

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development

    doi: 10.1002/dneu.22260

    Figure Lengend Snippet: Melanopsin ganglion cells apparently do not modulate starburst amacrine cell wave bursts. A. Whole cell patch recordings of ON starburst amacrine cells located in the ganglion cell layer of isolated whole-mounted retinas from early postnatal mice (P4

    Article Snippet: Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200).

    Techniques: Isolation, Mouse Assay

    Morphology of outer retinal dendrites of melanopsin immunopositive melanopsin ganglion cells at various developmental stages. A, B: Schematic diagrams of simple (A) and complex (B) outer retinal dendrites ascending from the main dendritic arbor of an

    Journal: Developmental neurobiology

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development

    doi: 10.1002/dneu.22260

    Figure Lengend Snippet: Morphology of outer retinal dendrites of melanopsin immunopositive melanopsin ganglion cells at various developmental stages. A, B: Schematic diagrams of simple (A) and complex (B) outer retinal dendrites ascending from the main dendritic arbor of an

    Article Snippet: Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200).

    Techniques:

    Complex outer retinal dendrites (CORDs) of melanopsin ganglion cells in relation to cone pedicles as seen in retinal wholemounts from P8 mice. All images reconstructed from z-stacks of deconvolved confocal sections. Panels A–I show one such dendrite,

    Journal: Developmental neurobiology

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development

    doi: 10.1002/dneu.22260

    Figure Lengend Snippet: Complex outer retinal dendrites (CORDs) of melanopsin ganglion cells in relation to cone pedicles as seen in retinal wholemounts from P8 mice. All images reconstructed from z-stacks of deconvolved confocal sections. Panels A–I show one such dendrite,

    Article Snippet: Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200).

    Techniques: Mouse Assay

    Development of dendritic stratification of melanopsin ganglion cells and starburst amacrine cells in the early postnatal period in mice. Epifluorescence photomicrographs of vertical retina sections in mice from three ages: P4 (A), P8 (B) and P10 (C).

    Journal: Developmental neurobiology

    Article Title: Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development

    doi: 10.1002/dneu.22260

    Figure Lengend Snippet: Development of dendritic stratification of melanopsin ganglion cells and starburst amacrine cells in the early postnatal period in mice. Epifluorescence photomicrographs of vertical retina sections in mice from three ages: P4 (A), P8 (B) and P10 (C).

    Article Snippet: Sections were incubated for 36–48 hours in a mixture of primary antibodies in blocking solution: rabbit anti-melanopsin (UF006; kind gift of Ignacio Provencio; 1:10,000 dilution) and goat anti-ChAT (Millipore AB144P; 1:200).

    Techniques: Mouse Assay

    The isoform γC4 is necessary for normal neuronal survival. Cryosections from P0 spinal cords were immunostained for the indicated markers (green; DAPI counterstain for nuclei, blue) A-B ) Immunostaing for NeuN revealed grossly smaller spinal cords and neuronal loss in C4KO mutants, but not 1R1 animals. C-D ) This was accompanied by reactive gliosis in C4KO. E-H ) Interneuron subtypes were analyzed as in Fig 4 ( E-F , FoxP2 and G-H , Pax2). K-L ) Ventral interneurons were drastically reduced in C4KO mutants; decreases in 1R1 mutants were statistically significant, but substantially more modest. I-J ) Apoptosis was increased in C4KO animals, but not in 1R1 mutants, as demonstrated by CC3 immunolabeling (quantified in M ). N-Q ) Whole mount retinas from 1R1 mutants were assayed for neuronal loss at 2 weeks of age and in adult as in Fig 6 . There were no reductions in ( N ) VGLUT3+ amacrine cells, ( O ) TH+ dopaminergic amacrine cells, or ( P ) Melanopsin+ ipRGCs. Q ) At 2 weeks of age, Brn3b+ RGCs were also not reduced, but their density was significantly lower in adult retinas. R ) Summary of phenotypes in the analyzed strains. Mutations that disrupted Pcdhgc4 resulted in high levels of cell death (+++) and postnatal lethality, while mutations that spared Pcdhgc4 resulted in little to no cell death and animal survival. Scale bar is 200 μm. * = p

    Journal: PLoS Genetics

    Article Title: CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4

    doi: 10.1371/journal.pgen.1008554

    Figure Lengend Snippet: The isoform γC4 is necessary for normal neuronal survival. Cryosections from P0 spinal cords were immunostained for the indicated markers (green; DAPI counterstain for nuclei, blue) A-B ) Immunostaing for NeuN revealed grossly smaller spinal cords and neuronal loss in C4KO mutants, but not 1R1 animals. C-D ) This was accompanied by reactive gliosis in C4KO. E-H ) Interneuron subtypes were analyzed as in Fig 4 ( E-F , FoxP2 and G-H , Pax2). K-L ) Ventral interneurons were drastically reduced in C4KO mutants; decreases in 1R1 mutants were statistically significant, but substantially more modest. I-J ) Apoptosis was increased in C4KO animals, but not in 1R1 mutants, as demonstrated by CC3 immunolabeling (quantified in M ). N-Q ) Whole mount retinas from 1R1 mutants were assayed for neuronal loss at 2 weeks of age and in adult as in Fig 6 . There were no reductions in ( N ) VGLUT3+ amacrine cells, ( O ) TH+ dopaminergic amacrine cells, or ( P ) Melanopsin+ ipRGCs. Q ) At 2 weeks of age, Brn3b+ RGCs were also not reduced, but their density was significantly lower in adult retinas. R ) Summary of phenotypes in the analyzed strains. Mutations that disrupted Pcdhgc4 resulted in high levels of cell death (+++) and postnatal lethality, while mutations that spared Pcdhgc4 resulted in little to no cell death and animal survival. Scale bar is 200 μm. * = p

    Article Snippet: Antibodies Primary antibodies used included the following: Guinea pig anti-VGLUT3 (1:10,000, Millipore), sheep anti-tyrosine hydroxylase (1:500, Millipore), rabbit anti-melanopsin (1:10,000, ATS), mouse anti-Brn3a (1:500, Millipore), goat anti-choline acetyltransferase (1:500, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-GFAP (1:500, Sigma), rabbit anti-FoxP2 (1:4000, Abcam), rabbit anti-Pax2 (1:200, Zymed), rabbit anti-cleaved caspase 3 (1:100, Cell Signaling Technologies), mouse anti-parvalbumin (1:500, Sigma), and mouse anti-GAPDH (1:500, Abcam).

    Techniques: Immunolabeling

    Reduced retinal neuron numbers in 13R1 mutants. Whole mount retinas from two-week-old mice were immunostained to label neuronal subtypes, imaged en face by confocal microscopy, and cell densities quantified. Analyzed cell types include ( A-E ) VGLUT3+ amacrine cells, ( F-J ) TH+ dopaminergic amacrine cells, ( K-O ) Melanopsin+ ipRGCs, and ( P-T ) Brn3a+ RGCs. For all four of the subtypes assayed, densities were significantly reduced in 13R1/cRKO retinas compared to wild-type. Densities were not reduced in either 3R1 or 3R2. Scale bar in D is 100 μm (for A-D ); scale bar in S is 300 μm (for F-S ). ** = p

    Journal: PLoS Genetics

    Article Title: CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4

    doi: 10.1371/journal.pgen.1008554

    Figure Lengend Snippet: Reduced retinal neuron numbers in 13R1 mutants. Whole mount retinas from two-week-old mice were immunostained to label neuronal subtypes, imaged en face by confocal microscopy, and cell densities quantified. Analyzed cell types include ( A-E ) VGLUT3+ amacrine cells, ( F-J ) TH+ dopaminergic amacrine cells, ( K-O ) Melanopsin+ ipRGCs, and ( P-T ) Brn3a+ RGCs. For all four of the subtypes assayed, densities were significantly reduced in 13R1/cRKO retinas compared to wild-type. Densities were not reduced in either 3R1 or 3R2. Scale bar in D is 100 μm (for A-D ); scale bar in S is 300 μm (for F-S ). ** = p

    Article Snippet: Antibodies Primary antibodies used included the following: Guinea pig anti-VGLUT3 (1:10,000, Millipore), sheep anti-tyrosine hydroxylase (1:500, Millipore), rabbit anti-melanopsin (1:10,000, ATS), mouse anti-Brn3a (1:500, Millipore), goat anti-choline acetyltransferase (1:500, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-GFAP (1:500, Sigma), rabbit anti-FoxP2 (1:4000, Abcam), rabbit anti-Pax2 (1:200, Zymed), rabbit anti-cleaved caspase 3 (1:100, Cell Signaling Technologies), mouse anti-parvalbumin (1:500, Sigma), and mouse anti-GAPDH (1:500, Abcam).

    Techniques: Mouse Assay, Confocal Microscopy

    Substantial loss of retinal thickness without layer disorganization in 13R1 mutants. A-C ) Cryosections taken in cross section through retinas of ( A ) wild type, ( B ) 13R1/cRKO, and ( C ) 3R2 mutants at two weeks of age were immunostained for SV2 to label the synaptic layers (outer and inner plexiform layers; OPL, IPL) and DAPI to label the inner and outer nuclear layers (ONL, INL) and retinal ganglion cell layer (RGL). The IPL and INL were both notably thinner in 13R1/cRKO than in wild type or 3R2 mutants. This was not accompanied by disorganization of neuronal subtype stratification within the IPL, as revealed by ( D-F ) Melanopsin and TH immunolabeling of ipRGCs and dopaminergic amacrine cells, respectively, and ( G-I ) VGLUT3 and ChAT immunolabeling of glutamatergic amacrine cells and starburst amacrine cells, respectively. Scale bar is 50 μm. Images are representative of at least 6 retinas per genotype analyzed.

    Journal: PLoS Genetics

    Article Title: CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4

    doi: 10.1371/journal.pgen.1008554

    Figure Lengend Snippet: Substantial loss of retinal thickness without layer disorganization in 13R1 mutants. A-C ) Cryosections taken in cross section through retinas of ( A ) wild type, ( B ) 13R1/cRKO, and ( C ) 3R2 mutants at two weeks of age were immunostained for SV2 to label the synaptic layers (outer and inner plexiform layers; OPL, IPL) and DAPI to label the inner and outer nuclear layers (ONL, INL) and retinal ganglion cell layer (RGL). The IPL and INL were both notably thinner in 13R1/cRKO than in wild type or 3R2 mutants. This was not accompanied by disorganization of neuronal subtype stratification within the IPL, as revealed by ( D-F ) Melanopsin and TH immunolabeling of ipRGCs and dopaminergic amacrine cells, respectively, and ( G-I ) VGLUT3 and ChAT immunolabeling of glutamatergic amacrine cells and starburst amacrine cells, respectively. Scale bar is 50 μm. Images are representative of at least 6 retinas per genotype analyzed.

    Article Snippet: Antibodies Primary antibodies used included the following: Guinea pig anti-VGLUT3 (1:10,000, Millipore), sheep anti-tyrosine hydroxylase (1:500, Millipore), rabbit anti-melanopsin (1:10,000, ATS), mouse anti-Brn3a (1:500, Millipore), goat anti-choline acetyltransferase (1:500, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-GFAP (1:500, Sigma), rabbit anti-FoxP2 (1:4000, Abcam), rabbit anti-Pax2 (1:200, Zymed), rabbit anti-cleaved caspase 3 (1:100, Cell Signaling Technologies), mouse anti-parvalbumin (1:500, Sigma), and mouse anti-GAPDH (1:500, Abcam).

    Techniques: Immunolabeling

    Pou4f2 Cre -mediated recombination within the retina. Cre-mediated recombination within the retina was assayed in Pou4f2 Cre/+ Ai9 mice by immunostaining retinal cryosections with a panel of markers. Channels are split out for better visualization. Markers are described in further detail in Table 1 . Abbreviations: mela = melanopsin, Calb = calbindin. Scale bars = 50 μm. Insets = 16 μm boxes.

    Journal: Molecular Vision

    Article Title: Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers

    doi:

    Figure Lengend Snippet: Pou4f2 Cre -mediated recombination within the retina. Cre-mediated recombination within the retina was assayed in Pou4f2 Cre/+ Ai9 mice by immunostaining retinal cryosections with a panel of markers. Channels are split out for better visualization. Markers are described in further detail in Table 1 . Abbreviations: mela = melanopsin, Calb = calbindin. Scale bars = 50 μm. Insets = 16 μm boxes.

    Article Snippet: Antibodies The following antibodies were used in this study: mouse anti-POU4F1/BRN3A (Millipore, Darmstadt, Germany; MAB1585; 1:100); goat anti-POU4F2/BRN3B (Santa Cruz Biotechnology, Santa Cruz, CA; sc-6026; 1:100); rabbit anti-melanopsin (generous gift from Ignacio Provencio; 1:10,000); rabbit anti-tyrosine hydroxylase (Millipore; AB152; 1:500); mouse anti-AP2α (Developmental Studies Hybridoma Bank, Trevor J. Williams; 3B5; 1:50); rabbit anti-calbindin (Swant, Marly, Switzerland; CB-38a; 1:1,000); goat anti-choline acetyltansferase (Millipore; AB144P; 1:250); rabbit anti-bNOS (Sigma, St. Louis, MO; N7280; 1:5,000); mouse anti-PKCα (Santa Cruz Biotechnology, sc-17769; 1:500); rabbit anti-cone arrestin (Millipore; AB15282; 1:10,000); mouse anti-glutamine synthetase (Millipore; MAB302; 1:1,000); mouse anti-calsenilin (Millipore; 05–756; 1:1,000); mouse anti-PAX6 (developed by Kawakami, Developmental Studies Hybridoma; 1:500); and rabbit anti-Cre (Millipore; 69,050-3; 1:500).

    Techniques: Mouse Assay, Immunostaining