rabbit anti m1 muscarinic receptor  (Alomone Labs)


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    Alomone Labs rabbit anti m1 muscarinic receptor
    Expression of M1 and M2 muscarinic receptors in gerbil AVCN. (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) <t>M1</t> muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
    Rabbit Anti M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti m1 muscarinic receptor/product/Alomone Labs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti m1 muscarinic receptor - by Bioz Stars, 2022-12
    92/100 stars

    Images

    1) Product Images from "Muscarinic modulation of M and h currents in gerbil spherical bushy cells"

    Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0226954

    Expression of M1 and M2 muscarinic receptors in gerbil AVCN. (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
    Figure Legend Snippet: Expression of M1 and M2 muscarinic receptors in gerbil AVCN. (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.

    Techniques Used: Expressing, Staining

    2) Product Images from "Muscarinic modulation of M and h currents in gerbil spherical bushy cells"

    Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0226954

    Expression of M1 and M2 muscarinic receptors in gerbil AVCN. (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
    Figure Legend Snippet: Expression of M1 and M2 muscarinic receptors in gerbil AVCN. (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.

    Techniques Used: Expressing, Staining

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    Alomone Labs anti chrm1 antibody
    Muscarinic 1 receptor (M1) activation regulates the activity of qNSCs in SVZ niche. A . Immunofluorescence staining for VCAM 1 (Purple) and <t>ChRM1</t> (green) in the SVZ niche from P30 C57BL/6J mice. B . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) from the SVZ NSCs culture. C . Western blot detection of pEGFR (PhosphoY1068) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures in proliferation media collected after 12 hours. D . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel C. *P = 0.0107, n=4, Unpaired t-test. E . Immunofluorescence staining for EdU (purple) and Ki67 (green) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in proliferation media collected after one day. F . Analysis of normalized quantification of Ki67 + cells per well in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel E. P = n.s., n=5, Unpaired t-test. G . Analysis of normalized quantification of EdU + /Ki67 + cells ratio per well in control and treated samples with carbachol (15μM) of SVZ NSCs cultures from panel E. **P = 0.0012, n=6, Unpaired t-test. H . Western blot detection of pEGFR(PhosphoY1068)/β-actin in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in differentiation media collected every 12 hours for 36 hours. I . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures from panel H. P
    Anti Chrm1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chrm1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chrm1 antibody - by Bioz Stars, 2022-12
    94/100 stars
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    Muscarinic 1 receptor (M1) activation regulates the activity of qNSCs in SVZ niche. A . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) in the SVZ niche from P30 C57BL/6J mice. B . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) from the SVZ NSCs culture. C . Western blot detection of pEGFR (PhosphoY1068) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures in proliferation media collected after 12 hours. D . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel C. *P = 0.0107, n=4, Unpaired t-test. E . Immunofluorescence staining for EdU (purple) and Ki67 (green) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in proliferation media collected after one day. F . Analysis of normalized quantification of Ki67 + cells per well in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel E. P = n.s., n=5, Unpaired t-test. G . Analysis of normalized quantification of EdU + /Ki67 + cells ratio per well in control and treated samples with carbachol (15μM) of SVZ NSCs cultures from panel E. **P = 0.0012, n=6, Unpaired t-test. H . Western blot detection of pEGFR(PhosphoY1068)/β-actin in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in differentiation media collected every 12 hours for 36 hours. I . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures from panel H. P

    Journal: bioRxiv

    Article Title: Uncovering a neural circuit controlling adult quiescent neural stem cell activation in the subventricular zone

    doi: 10.1101/2021.12.27.474262

    Figure Lengend Snippet: Muscarinic 1 receptor (M1) activation regulates the activity of qNSCs in SVZ niche. A . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) in the SVZ niche from P30 C57BL/6J mice. B . Immunofluorescence staining for VCAM 1 (Purple) and ChRM1 (green) from the SVZ NSCs culture. C . Western blot detection of pEGFR (PhosphoY1068) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures in proliferation media collected after 12 hours. D . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel C. *P = 0.0107, n=4, Unpaired t-test. E . Immunofluorescence staining for EdU (purple) and Ki67 (green) in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in proliferation media collected after one day. F . Analysis of normalized quantification of Ki67 + cells per well in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20 μM) of SVZ NSCs cultures from panel E. P = n.s., n=5, Unpaired t-test. G . Analysis of normalized quantification of EdU + /Ki67 + cells ratio per well in control and treated samples with carbachol (15μM) of SVZ NSCs cultures from panel E. **P = 0.0012, n=6, Unpaired t-test. H . Western blot detection of pEGFR(PhosphoY1068)/β-actin in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures in differentiation media collected every 12 hours for 36 hours. I . Analysis of normalized quantification of pEGFR(PhosphoY1068)/β-actin protein intensity in treated samples with carbachol (15μM) and carbachol (15μM)/pirenzepine (20μM) of SVZ NSCs cultures from panel H. P

    Article Snippet: We used primary antibodies to GFP (#GFP-1020, 1:400, AVES lab), Choline Acetyltransferase Antibody (#AB144P, 1:250, Millipore sigma), tdTomato [16D7] (#EST203, 1:200, Kerafast), RFP (#600-401-379, 1:250, Rockland), Calretinin (#ab702, 1:200, Abcam), calretinin (#6B3, 1:250, Swant), Calretinin (#MCA-3G9, 1:250, EnCor Biotechnology), RFP (#ab62341, 1:200, Abcam), Doublecortin (#AB2253, 1:250, Millipore), Doublecortin (#4604S, 1:200, Cell Signaling Technology), Ki67 (ab15580, 1:250: Abcam), Ki67 (#CPCA-Ki67, 1:200, EnCor Biotechnology), beta Actin HRP (#MA515739HRP (BA3R), 1:5000, Thermo Fisher Scientific), HRP-conjugated Beta Actin (#HRP-60008, 1:5000, Proteintech), EGFR (phospho Y1068) (#ab5644, 1:250, Abcam), EGFR (phospho Y1068) [Y38] (#ab32430, 1:250, Abcam), CD106 (#550547, 1:100, BD Diagnostic Systems), GFAP (#GFAP, 1:500, Aves Labs), M1 Muscarinic Receptor (#AMR-001, Alomone Labs).

    Techniques: Activation Assay, Activity Assay, Immunofluorescence, Staining, Mouse Assay, Western Blot