rabbit anti lmo4  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    Caspase 3 Assay Kit Fluorometric
    Description:

    Catalog Number:
    AB39383
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam rabbit anti lmo4
    Growth pattern of <t>LMO4</t> knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.

    https://www.bioz.com/result/rabbit anti lmo4/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti lmo4 - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth"

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    Journal: Journal of cellular biochemistry

    doi: 10.1002/jcb.26529

    Growth pattern of LMO4 knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.
    Figure Legend Snippet: Growth pattern of LMO4 knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.

    Techniques Used: Knock-Out, MTT Assay, Standard Deviation

    Knockout of LMO4 in UB/OC1 cells. A, Green fluorescent cells indicate the presence of GFP in UB/OC1 cells transfected with CRISPR/Cas9 knockout plasmid. GFP is not detected in the wild-type cells. The images are representative of three biological replicates. Scale bar represents 200 μm. B, Immunoblots indicate that LMO4 protein is not detected in the knockout cells. Actin is used for normalization. The images are representative of three biological replicates. C, Modified DNA sequence of target locus in the knockout cells indicates a 6 base pair deletion.
    Figure Legend Snippet: Knockout of LMO4 in UB/OC1 cells. A, Green fluorescent cells indicate the presence of GFP in UB/OC1 cells transfected with CRISPR/Cas9 knockout plasmid. GFP is not detected in the wild-type cells. The images are representative of three biological replicates. Scale bar represents 200 μm. B, Immunoblots indicate that LMO4 protein is not detected in the knockout cells. Actin is used for normalization. The images are representative of three biological replicates. C, Modified DNA sequence of target locus in the knockout cells indicates a 6 base pair deletion.

    Techniques Used: Knock-Out, Transfection, CRISPR, Plasmid Preparation, Western Blot, Modification, Sequencing

    Migratory potential of LMO4 knockout UB/OC1 cells. Wound healing assay suggests that the migratory potential of LMO4 knockout cells is relatively slower than that of the control cells. The wound closure in control cells after 24 h is considered as 100%. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed t -tests, * indicates P
    Figure Legend Snippet: Migratory potential of LMO4 knockout UB/OC1 cells. Wound healing assay suggests that the migratory potential of LMO4 knockout cells is relatively slower than that of the control cells. The wound closure in control cells after 24 h is considered as 100%. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed t -tests, * indicates P

    Techniques Used: Knock-Out, Wound Healing Assay, Standard Deviation, One-tailed Test

    Differentiation of LMO4 knockout UB/OC1 cells. Myosin Vila, a biomarker of hair cells, is detected in both the wild-type and knockout cells suggesting that the knockout of LMO4 does not alter the differentiation of UB/OC1 cells. Red staining indicates immunoreactivity to antimyosin Vila, green indicates actin staining with phalloidin, while blue indicates nuclear staining with DAPI. The images are representative of three biological replicates. Scale bar represents 20 μm.
    Figure Legend Snippet: Differentiation of LMO4 knockout UB/OC1 cells. Myosin Vila, a biomarker of hair cells, is detected in both the wild-type and knockout cells suggesting that the knockout of LMO4 does not alter the differentiation of UB/OC1 cells. Red staining indicates immunoreactivity to antimyosin Vila, green indicates actin staining with phalloidin, while blue indicates nuclear staining with DAPI. The images are representative of three biological replicates. Scale bar represents 20 μm.

    Techniques Used: Knock-Out, Biomarker Assay, Staining

    Viability of LMO4 knockout cells after cisplatin treatment. Cell counts indicate that cisplatin treatment decreases the viability of UB/OC1 cells. However, the number of viable cells was much lower in the LMO4 knockout cells relative to the control and wild-type cells. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed i-tests, * indicates P
    Figure Legend Snippet: Viability of LMO4 knockout cells after cisplatin treatment. Cell counts indicate that cisplatin treatment decreases the viability of UB/OC1 cells. However, the number of viable cells was much lower in the LMO4 knockout cells relative to the control and wild-type cells. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed i-tests, * indicates P

    Techniques Used: Knock-Out, Standard Deviation, One-tailed Test

    Schematic of the experimental design for generating LMO4 knockout UB/OC1 cells. UB/OC1 cells were transfected with LMO4 CRISPR/Cas9 knockout plasmids containing target-specific guide RNAs and GFP. Successful transfection of the plasmid was verified by the expression of GFP. The transfected cells containing GFP were isolated by cell sorting and propagated for further analysis.
    Figure Legend Snippet: Schematic of the experimental design for generating LMO4 knockout UB/OC1 cells. UB/OC1 cells were transfected with LMO4 CRISPR/Cas9 knockout plasmids containing target-specific guide RNAs and GFP. Successful transfection of the plasmid was verified by the expression of GFP. The transfected cells containing GFP were isolated by cell sorting and propagated for further analysis.

    Techniques Used: Knock-Out, Transfection, CRISPR, Plasmid Preparation, Expressing, Isolation, FACS

    Related Articles

    Activity Assay:

    Article Title: Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)
    Article Snippet: Caspase-3 protein activity assay (an indicator of cell death/apoptosis) For estimating caspase-3 activity, five honey bees were sampled and pooled from each of the experimental cages (for all treatments and controls) and homogenized and centrifuged as described above for ROS/RNS assays. .. Apoptosis was estimated by detecting the increase in caspase-3 activity using the fluorometric caspase-3 assay (Abcam, Cambridge, USA) following manufacturer’s protocols [ , ]. .. Sample supernatants were assayed in duplicate and fluorescence was measured at 400 nm excitation and 505 nm emission by a microplate reader (Biotek Synergy 2, BioTek Instruments, USA).

    Article Title: MicroRNA-320 Enhances Radiosensitivity of Glioma Through Down-Regulation of Sirtuin Type 1 by Directly Targeting Forkhead Box Protein M1
    Article Snippet: Then cells were double-stained with Annexin V-FITC (5 μl) and PI (5 μl, 50 μg/ml) for 5 min at room temperature in the dark, followed by the quantification using the flow cytometry (BD Biosciences, San Jose, CA, USA). .. Caspase 3 activity was detected using Caspase 3 assay kit (Abcam, Cambridge, UK) according to the protocol of manufacturer and the optical density value was measured at the wavelength of 400 nm. .. Western Blot Assay The total protein was extracted using RIPA buffer (Beyotime, Shanghai, China) containing cocktail (Roche Diagnostics, Basel, Switzerland) and quantified using a BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).

    Article Title: Possible Involvement of Nitric Oxide in Enhanced Liver Injury and Fibrogenesis during Cholestasis in Cytoglobin-deficient Mice
    Article Snippet: Caspase 3 activity assay The caspase 3 activity is one of important hallmarks to assess apoptosis pathway. .. The caspase 3 activity was measured by using Caspase 3 assay kit (Abcam, Cambrige, UK) according to the assay protocol. .. Briefly, liver tissue was homogenized on ice in lysis buffer, then, incubated with the substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) which emits blue light.

    Article Title: Challenging inflammatory process at molecular, cellular and in vivo levels via some new pyrazolyl thiazolones
    Article Snippet: Afterwards, adherent macrophages were detached using 2.5% trypsin in PBS (abcam, Cambridge, UK). .. Caspase activity was assessed using the Caspase-3 Assay Kit (Abcam, Cambridge, UK). ..

    Article Title: SHP-1 is a target of regorafenib in colorectal cancer
    Article Snippet: Apoptotic cell death was determined using the Cell Death Detection ELISAPLUS kit (Roche Applied Sciences, Germany). .. Caspase-3 activity was measured using the Caspase 3 Assay Kit (Abcam, Cambrige, MA). .. Cell proliferation assay After treatment with regorafenib at the indicated doses for 2 days, cell proliferation was measured using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.

    Caspase-3 Assay:

    Article Title: Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)
    Article Snippet: Caspase-3 protein activity assay (an indicator of cell death/apoptosis) For estimating caspase-3 activity, five honey bees were sampled and pooled from each of the experimental cages (for all treatments and controls) and homogenized and centrifuged as described above for ROS/RNS assays. .. Apoptosis was estimated by detecting the increase in caspase-3 activity using the fluorometric caspase-3 assay (Abcam, Cambridge, USA) following manufacturer’s protocols [ , ]. .. Sample supernatants were assayed in duplicate and fluorescence was measured at 400 nm excitation and 505 nm emission by a microplate reader (Biotek Synergy 2, BioTek Instruments, USA).

    Article Title: MicroRNA-320 Enhances Radiosensitivity of Glioma Through Down-Regulation of Sirtuin Type 1 by Directly Targeting Forkhead Box Protein M1
    Article Snippet: Then cells were double-stained with Annexin V-FITC (5 μl) and PI (5 μl, 50 μg/ml) for 5 min at room temperature in the dark, followed by the quantification using the flow cytometry (BD Biosciences, San Jose, CA, USA). .. Caspase 3 activity was detected using Caspase 3 assay kit (Abcam, Cambridge, UK) according to the protocol of manufacturer and the optical density value was measured at the wavelength of 400 nm. .. Western Blot Assay The total protein was extracted using RIPA buffer (Beyotime, Shanghai, China) containing cocktail (Roche Diagnostics, Basel, Switzerland) and quantified using a BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).

    Article Title: Possible Involvement of Nitric Oxide in Enhanced Liver Injury and Fibrogenesis during Cholestasis in Cytoglobin-deficient Mice
    Article Snippet: Caspase 3 activity assay The caspase 3 activity is one of important hallmarks to assess apoptosis pathway. .. The caspase 3 activity was measured by using Caspase 3 assay kit (Abcam, Cambrige, UK) according to the assay protocol. .. Briefly, liver tissue was homogenized on ice in lysis buffer, then, incubated with the substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) which emits blue light.

    Article Title: Lentiviral delivery of novel fusion protein IL12/FasTI for cancer immune/gene therapy
    Article Snippet: At this point one million NK92 cells were added to all the cells and incubated overnight for coculture. .. After removing suspension NK92 cells, apoptosis of the tumor cells was determined by a caspase 3 assay kit (abcam) following the manufacturer’s instruction. ..

    Article Title: Challenging inflammatory process at molecular, cellular and in vivo levels via some new pyrazolyl thiazolones
    Article Snippet: Afterwards, adherent macrophages were detached using 2.5% trypsin in PBS (abcam, Cambridge, UK). .. Caspase activity was assessed using the Caspase-3 Assay Kit (Abcam, Cambridge, UK). ..

    Article Title: SHP-1 is a target of regorafenib in colorectal cancer
    Article Snippet: Apoptotic cell death was determined using the Cell Death Detection ELISAPLUS kit (Roche Applied Sciences, Germany). .. Caspase-3 activity was measured using the Caspase 3 Assay Kit (Abcam, Cambrige, MA). .. Cell proliferation assay After treatment with regorafenib at the indicated doses for 2 days, cell proliferation was measured using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.

    Incubation:

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth
    Article Snippet: The protein concentration was determined by Bradford protein assay and 30 of protein was loaded on 4–20% Mini-Protean TGX gel (456–1093, Bio-Rad Laboratories, Inc., Hercules, CA). .. The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA). .. After incubation with peroxidase-conjugated anti-rabbit secondary antibody (32460, Thermo Fisher Scientific), the blots were developed using chemiluminescence detection reagent (34076, Thermo Fisher Scientific), and visualized using FluorChem E System (ProteinSimple, San Jose, CA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Abcam rabbit anti lmo4
    Growth pattern of <t>LMO4</t> knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.
    Rabbit Anti Lmo4, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti lmo4/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti lmo4 - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    93
    Abcam anti lmo4
    miR-409-3p is enriched in CSMN, and it represses the CPN-expressed and CSMN-excluded transcriptional regulator <t>LMO4.</t> (A) miR-409-3p is six-fold enriched in CSMN vs. CPN at P2 by qPCR. Error bars represent SEM. (B) LMO4 is enriched in CPN vs. CSMN in late embryonic and early postnatal life by microarray analysis. Error bars represent SEM. (C) Sequence alignments demonstrate that miR-409-3p is predicted to target two sites in the LMO4 3’ UTR. Seed sequence base-pairing is shown in red. (D) miR-409-3p oligonucleotides repress a LMO4 3’UTR luciferase reporter gene bearing wild-type, but not mismatch, miR-409-3p target sequences. Scrambled miRNA does not repress the LMO4 3’UTR luciferase reporter. Error bars represent SEM. * p
    Anti Lmo4, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti lmo4/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti lmo4 - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    99
    Abcam rabbit anti human lmo4 antibody
    <t>LMO4</t> levels are significantly associated with patient overall survival (OS) in different tumor stages and sizes. (A,B) OS of patients with high or low-LMO4 expression when further divided into tumor-node-metastasis (TNM) I–II and TNM III–IV subgroups; (C,D) OS rates of patients with tumor size ﹤3 cm and tumor size ≥3 cm, respectively.
    Rabbit Anti Human Lmo4 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human lmo4 antibody/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human lmo4 antibody - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Growth pattern of LMO4 knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Growth pattern of LMO4 knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, MTT Assay, Standard Deviation

    Knockout of LMO4 in UB/OC1 cells. A, Green fluorescent cells indicate the presence of GFP in UB/OC1 cells transfected with CRISPR/Cas9 knockout plasmid. GFP is not detected in the wild-type cells. The images are representative of three biological replicates. Scale bar represents 200 μm. B, Immunoblots indicate that LMO4 protein is not detected in the knockout cells. Actin is used for normalization. The images are representative of three biological replicates. C, Modified DNA sequence of target locus in the knockout cells indicates a 6 base pair deletion.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Knockout of LMO4 in UB/OC1 cells. A, Green fluorescent cells indicate the presence of GFP in UB/OC1 cells transfected with CRISPR/Cas9 knockout plasmid. GFP is not detected in the wild-type cells. The images are representative of three biological replicates. Scale bar represents 200 μm. B, Immunoblots indicate that LMO4 protein is not detected in the knockout cells. Actin is used for normalization. The images are representative of three biological replicates. C, Modified DNA sequence of target locus in the knockout cells indicates a 6 base pair deletion.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Transfection, CRISPR, Plasmid Preparation, Western Blot, Modification, Sequencing

    Migratory potential of LMO4 knockout UB/OC1 cells. Wound healing assay suggests that the migratory potential of LMO4 knockout cells is relatively slower than that of the control cells. The wound closure in control cells after 24 h is considered as 100%. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed t -tests, * indicates P

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Migratory potential of LMO4 knockout UB/OC1 cells. Wound healing assay suggests that the migratory potential of LMO4 knockout cells is relatively slower than that of the control cells. The wound closure in control cells after 24 h is considered as 100%. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed t -tests, * indicates P

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Wound Healing Assay, Standard Deviation, One-tailed Test

    Differentiation of LMO4 knockout UB/OC1 cells. Myosin Vila, a biomarker of hair cells, is detected in both the wild-type and knockout cells suggesting that the knockout of LMO4 does not alter the differentiation of UB/OC1 cells. Red staining indicates immunoreactivity to antimyosin Vila, green indicates actin staining with phalloidin, while blue indicates nuclear staining with DAPI. The images are representative of three biological replicates. Scale bar represents 20 μm.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Differentiation of LMO4 knockout UB/OC1 cells. Myosin Vila, a biomarker of hair cells, is detected in both the wild-type and knockout cells suggesting that the knockout of LMO4 does not alter the differentiation of UB/OC1 cells. Red staining indicates immunoreactivity to antimyosin Vila, green indicates actin staining with phalloidin, while blue indicates nuclear staining with DAPI. The images are representative of three biological replicates. Scale bar represents 20 μm.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Biomarker Assay, Staining

    Viability of LMO4 knockout cells after cisplatin treatment. Cell counts indicate that cisplatin treatment decreases the viability of UB/OC1 cells. However, the number of viable cells was much lower in the LMO4 knockout cells relative to the control and wild-type cells. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed i-tests, * indicates P

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Viability of LMO4 knockout cells after cisplatin treatment. Cell counts indicate that cisplatin treatment decreases the viability of UB/OC1 cells. However, the number of viable cells was much lower in the LMO4 knockout cells relative to the control and wild-type cells. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed i-tests, * indicates P

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Standard Deviation, One-tailed Test

    Schematic of the experimental design for generating LMO4 knockout UB/OC1 cells. UB/OC1 cells were transfected with LMO4 CRISPR/Cas9 knockout plasmids containing target-specific guide RNAs and GFP. Successful transfection of the plasmid was verified by the expression of GFP. The transfected cells containing GFP were isolated by cell sorting and propagated for further analysis.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Schematic of the experimental design for generating LMO4 knockout UB/OC1 cells. UB/OC1 cells were transfected with LMO4 CRISPR/Cas9 knockout plasmids containing target-specific guide RNAs and GFP. Successful transfection of the plasmid was verified by the expression of GFP. The transfected cells containing GFP were isolated by cell sorting and propagated for further analysis.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Transfection, CRISPR, Plasmid Preparation, Expressing, Isolation, FACS

    miR-409-3p is enriched in CSMN, and it represses the CPN-expressed and CSMN-excluded transcriptional regulator LMO4. (A) miR-409-3p is six-fold enriched in CSMN vs. CPN at P2 by qPCR. Error bars represent SEM. (B) LMO4 is enriched in CPN vs. CSMN in late embryonic and early postnatal life by microarray analysis. Error bars represent SEM. (C) Sequence alignments demonstrate that miR-409-3p is predicted to target two sites in the LMO4 3’ UTR. Seed sequence base-pairing is shown in red. (D) miR-409-3p oligonucleotides repress a LMO4 3’UTR luciferase reporter gene bearing wild-type, but not mismatch, miR-409-3p target sequences. Scrambled miRNA does not repress the LMO4 3’UTR luciferase reporter. Error bars represent SEM. * p

    Journal: bioRxiv

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections, Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G( Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G(

    doi: 10.1101/2020.09.08.286955

    Figure Lengend Snippet: miR-409-3p is enriched in CSMN, and it represses the CPN-expressed and CSMN-excluded transcriptional regulator LMO4. (A) miR-409-3p is six-fold enriched in CSMN vs. CPN at P2 by qPCR. Error bars represent SEM. (B) LMO4 is enriched in CPN vs. CSMN in late embryonic and early postnatal life by microarray analysis. Error bars represent SEM. (C) Sequence alignments demonstrate that miR-409-3p is predicted to target two sites in the LMO4 3’ UTR. Seed sequence base-pairing is shown in red. (D) miR-409-3p oligonucleotides repress a LMO4 3’UTR luciferase reporter gene bearing wild-type, but not mismatch, miR-409-3p target sequences. Scrambled miRNA does not repress the LMO4 3’UTR luciferase reporter. Error bars represent SEM. * p

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton-X100, 2% sheep serum, and 1% BSA, and cells were then incubated with primary antibodies against cell specific markers: anti-CTIP2 (AbCam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (AbCam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (AbCam, rabbit polyclonal, dilution 1:200).

    Techniques: Real-time Polymerase Chain Reaction, Microarray, Sequencing, Luciferase

    CSMN-enriched miRNAs are encoded at a genomic cluster that co-evolved with motor cortex and corpus callosum. (A) Schematic of the mouse 12qF1 locus highlighting miRNAs identified as enriched in CSMN (magenta). Meg3, anti-Rtl1, Rian, and Mirg are incompletely characterized genes encoding long RNAs that give rise to the eutherian-specific clustered 12qF1 microRNAs. Dlk1, Rtl1, and Dio3 are protein-coding genes at 12qF1 that are conserved in pre-eutherian mammals. (B) Schematic of the vertebrate LMO4 3’UTR depicts that the proximal portion (grey) is well-conserved among characterized vertebrate LMO4 mRNAs, whereas the distal portion of the eutherian LMO4 3’UTR (black) is absent from all characterized chicken LMO4 mRNAs and all marsupial LMO4 genes. The positions of predicted CSMN-enriched 12qF1 miRNA target sites, concentrated in the distal/eutherian portion of the LMO4 3’ UTR, are indicated by colored bars. Multiple sequence alignments illustrate that mir-409-3p site 1 is well conserved among vertebrates, whereas site 2 appears to be well conserved only among eutherians. Predicted mRNAs are italicized; characterized mRNAs are not italicized. (C) Individual miRNAs repress multiple targets, and clustered miRNAs cooperatively repress shared targets. Six CSMN-enriched 12qF1 cluster miRNAs, in addition to miR-409-3p, are predicted to cooperatively repress LMO4. (D) Model depicting deep layer CPN and eutherian CSMN derived from ancestral pre-eutherian CSMN via expansion of gene expression to generate CPN as a new projection neuron subtype, and pruning of this expansion in eutherian CSMN via miRNA-mediated repression of gene expression.

    Journal: bioRxiv

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections, Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G( Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G(

    doi: 10.1101/2020.09.08.286955

    Figure Lengend Snippet: CSMN-enriched miRNAs are encoded at a genomic cluster that co-evolved with motor cortex and corpus callosum. (A) Schematic of the mouse 12qF1 locus highlighting miRNAs identified as enriched in CSMN (magenta). Meg3, anti-Rtl1, Rian, and Mirg are incompletely characterized genes encoding long RNAs that give rise to the eutherian-specific clustered 12qF1 microRNAs. Dlk1, Rtl1, and Dio3 are protein-coding genes at 12qF1 that are conserved in pre-eutherian mammals. (B) Schematic of the vertebrate LMO4 3’UTR depicts that the proximal portion (grey) is well-conserved among characterized vertebrate LMO4 mRNAs, whereas the distal portion of the eutherian LMO4 3’UTR (black) is absent from all characterized chicken LMO4 mRNAs and all marsupial LMO4 genes. The positions of predicted CSMN-enriched 12qF1 miRNA target sites, concentrated in the distal/eutherian portion of the LMO4 3’ UTR, are indicated by colored bars. Multiple sequence alignments illustrate that mir-409-3p site 1 is well conserved among vertebrates, whereas site 2 appears to be well conserved only among eutherians. Predicted mRNAs are italicized; characterized mRNAs are not italicized. (C) Individual miRNAs repress multiple targets, and clustered miRNAs cooperatively repress shared targets. Six CSMN-enriched 12qF1 cluster miRNAs, in addition to miR-409-3p, are predicted to cooperatively repress LMO4. (D) Model depicting deep layer CPN and eutherian CSMN derived from ancestral pre-eutherian CSMN via expansion of gene expression to generate CPN as a new projection neuron subtype, and pruning of this expansion in eutherian CSMN via miRNA-mediated repression of gene expression.

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton-X100, 2% sheep serum, and 1% BSA, and cells were then incubated with primary antibodies against cell specific markers: anti-CTIP2 (AbCam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (AbCam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (AbCam, rabbit polyclonal, dilution 1:200).

    Techniques: Sequencing, Derivative Assay, Expressing

    miR-409-3p loss of function does not alter endogenous LMO4 expression in cultured embryonic cortical neurons. miR-409-3p antisense loss-of-function (LOF) in cultured embryonic cortical neurons results in no significant change in expression of endogenous LMO4, compared to scrambled control, by immunocytochemical analysis. Error bars represent SEM. n.s. not statistically significant.

    Journal: bioRxiv

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections, Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G( Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G(

    doi: 10.1101/2020.09.08.286955

    Figure Lengend Snippet: miR-409-3p loss of function does not alter endogenous LMO4 expression in cultured embryonic cortical neurons. miR-409-3p antisense loss-of-function (LOF) in cultured embryonic cortical neurons results in no significant change in expression of endogenous LMO4, compared to scrambled control, by immunocytochemical analysis. Error bars represent SEM. n.s. not statistically significant.

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton-X100, 2% sheep serum, and 1% BSA, and cells were then incubated with primary antibodies against cell specific markers: anti-CTIP2 (AbCam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (AbCam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (AbCam, rabbit polyclonal, dilution 1:200).

    Techniques: Expressing, Cell Culture

    Overexpression of LMO4 ORF alone does not significantly affect CTIP2 or SATB2 expression in cultured embryonic cortical neurons. Overexpression of the LMO4 ORF in cultured embryonic cortical neurons results in no significant change in the percent CTIP2+/GFP+ neurons (CSMN), nor in the percent SATB2+/CTIP2−/GFP+ neurons (CPN), compared to control. Error bars represent SEM. *p

    Journal: bioRxiv

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections, Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G( Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G(

    doi: 10.1101/2020.09.08.286955

    Figure Lengend Snippet: Overexpression of LMO4 ORF alone does not significantly affect CTIP2 or SATB2 expression in cultured embryonic cortical neurons. Overexpression of the LMO4 ORF in cultured embryonic cortical neurons results in no significant change in the percent CTIP2+/GFP+ neurons (CSMN), nor in the percent SATB2+/CTIP2−/GFP+ neurons (CPN), compared to control. Error bars represent SEM. *p

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton-X100, 2% sheep serum, and 1% BSA, and cells were then incubated with primary antibodies against cell specific markers: anti-CTIP2 (AbCam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (AbCam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (AbCam, rabbit polyclonal, dilution 1:200).

    Techniques: Over Expression, Expressing, Cell Culture

    miR-409-3p promotes CSMN subtype identity, and inhibits CPN subtype identity, in part via LMO4 repression. (A) Representative fluorescence micrographs of embryonic cortical cultures illustrate an increase in the percent CTIP2+/GFP+ neurons (CSMN) with miR-409-3p GOF, and a decrease in the percent CTIP2+/GFP+ neurons with miR-409-3p LOF. Scale bar, 50μm. (B) miR-409-3p overexpression gain-of-function (GOF) increases the percent CTIP2+/GFP+ neurons (CSMN), and miR-409-3p antisense loss-of-function (LOF) decreases the percent CTIP2+/GFP+ neurons, compared to scrambled control in embryonic cortical cultures. Overexpression of the LMO4 open reading frame (ORF) reverses the miR-409-3p GOF phenotype in embryonic cortical cultures. (C) miR-409-3p GOF decreases the percent SATB2+/CTIP2−/GFP+ neurons (CPN) compared to scrambled controls in embryonic cortical cultures. Overexpression of the LMO4 ORF reverses the miR-409-3p GOF phenotype. Error bars represent SEM. *p

    Journal: bioRxiv

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections, Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G( Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G(

    doi: 10.1101/2020.09.08.286955

    Figure Lengend Snippet: miR-409-3p promotes CSMN subtype identity, and inhibits CPN subtype identity, in part via LMO4 repression. (A) Representative fluorescence micrographs of embryonic cortical cultures illustrate an increase in the percent CTIP2+/GFP+ neurons (CSMN) with miR-409-3p GOF, and a decrease in the percent CTIP2+/GFP+ neurons with miR-409-3p LOF. Scale bar, 50μm. (B) miR-409-3p overexpression gain-of-function (GOF) increases the percent CTIP2+/GFP+ neurons (CSMN), and miR-409-3p antisense loss-of-function (LOF) decreases the percent CTIP2+/GFP+ neurons, compared to scrambled control in embryonic cortical cultures. Overexpression of the LMO4 open reading frame (ORF) reverses the miR-409-3p GOF phenotype in embryonic cortical cultures. (C) miR-409-3p GOF decreases the percent SATB2+/CTIP2−/GFP+ neurons (CPN) compared to scrambled controls in embryonic cortical cultures. Overexpression of the LMO4 ORF reverses the miR-409-3p GOF phenotype. Error bars represent SEM. *p

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton-X100, 2% sheep serum, and 1% BSA, and cells were then incubated with primary antibodies against cell specific markers: anti-CTIP2 (AbCam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (AbCam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (AbCam, rabbit polyclonal, dilution 1:200).

    Techniques: Fluorescence, Over Expression

    miR-409-3p is enriched in CSMN, and it represses the CPN-expressed and CSMN-excluded transcriptional regulator LMO4. ( A ) miR-409-3p is sixfold enriched in CSMN vs. CPN at P2 by qPCR. Error bars represent SEM. ( B ) LMO4 is enriched in CPN vs. CSMN in late embryonic and early postnatal life by microarray analysis. Error bars represent SEM. ( C ) Sequence alignments demonstrate that miR-409-3p is predicted to target two sites in the LMO4 3′ UTR. Seed sequence base pairing is shown in red. ( D ) miR-409-3p oligonucleotides repress a LMO4 3′ UTR luciferase reporter gene bearing wild-type, but not mismatch, miR-409-3p target sequences. Scrambled miRNA does not repress the LMO4 3′ UTR luciferase reporter. Error bars represent SEM. * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections

    doi: 10.1073/pnas.2006700117

    Figure Lengend Snippet: miR-409-3p is enriched in CSMN, and it represses the CPN-expressed and CSMN-excluded transcriptional regulator LMO4. ( A ) miR-409-3p is sixfold enriched in CSMN vs. CPN at P2 by qPCR. Error bars represent SEM. ( B ) LMO4 is enriched in CPN vs. CSMN in late embryonic and early postnatal life by microarray analysis. Error bars represent SEM. ( C ) Sequence alignments demonstrate that miR-409-3p is predicted to target two sites in the LMO4 3′ UTR. Seed sequence base pairing is shown in red. ( D ) miR-409-3p oligonucleotides repress a LMO4 3′ UTR luciferase reporter gene bearing wild-type, but not mismatch, miR-409-3p target sequences. Scrambled miRNA does not repress the LMO4 3′ UTR luciferase reporter. Error bars represent SEM. * P

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton X-100, 2% sheep serum, and 1% bovine serum albumin, and cells were then incubated with primary antibodies against cell-specific markers: anti-CTIP2 (Abcam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (Abcam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (Abcam, rabbit polyclonal, dilution 1:200).

    Techniques: Real-time Polymerase Chain Reaction, Microarray, Sequencing, Luciferase

    miR-409-3p promotes CSMN subtype identity, and inhibits CPN subtype identity, in part via LMO4 repression. ( A ) Representative fluorescence micrographs of embryonic cortical cultures illustrate an increase in the percent CTIP2 + /GFP + neurons (CSMN) with miR-409-3p GOF, and a decrease in the percent CTIP2 + /GFP + neurons with miR-409-3p LOF. (Scale bar, 50 μm.) ( B ) miR-409-3p overexpression GOF increases the percent CTIP2 + /GFP + neurons (CSMN), and miR-409-3p antisense LOF decreases the percent CTIP2 + /GFP + neurons, compared to scrambled control in embryonic cortical cultures. Overexpression of the LMO4 ORF reverses the miR-409-3p GOF phenotype in embryonic cortical cultures. ( C ) miR-409-3p GOF decreases the percent SATB2 + /CTIP2 − /GFP + neurons (CPN) compared to scrambled controls in embryonic cortical cultures. Overexpression of the LMO4 ORF reverses the miR-409-3p GOF phenotype. Error bars represent SEM. * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections

    doi: 10.1073/pnas.2006700117

    Figure Lengend Snippet: miR-409-3p promotes CSMN subtype identity, and inhibits CPN subtype identity, in part via LMO4 repression. ( A ) Representative fluorescence micrographs of embryonic cortical cultures illustrate an increase in the percent CTIP2 + /GFP + neurons (CSMN) with miR-409-3p GOF, and a decrease in the percent CTIP2 + /GFP + neurons with miR-409-3p LOF. (Scale bar, 50 μm.) ( B ) miR-409-3p overexpression GOF increases the percent CTIP2 + /GFP + neurons (CSMN), and miR-409-3p antisense LOF decreases the percent CTIP2 + /GFP + neurons, compared to scrambled control in embryonic cortical cultures. Overexpression of the LMO4 ORF reverses the miR-409-3p GOF phenotype in embryonic cortical cultures. ( C ) miR-409-3p GOF decreases the percent SATB2 + /CTIP2 − /GFP + neurons (CPN) compared to scrambled controls in embryonic cortical cultures. Overexpression of the LMO4 ORF reverses the miR-409-3p GOF phenotype. Error bars represent SEM. * P

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton X-100, 2% sheep serum, and 1% bovine serum albumin, and cells were then incubated with primary antibodies against cell-specific markers: anti-CTIP2 (Abcam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (Abcam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (Abcam, rabbit polyclonal, dilution 1:200).

    Techniques: Fluorescence, Over Expression

    CSMN-enriched miRNAs are encoded at a genomic cluster that coevolved with motor cortex and corpus callosum. ( A ) Schematic of the mouse 12qF1 locus highlighting miRNAs identified as enriched in CSMN (magenta). Meg3, anti-Rtl1, Rian, and Mirg are incompletely characterized genes encoding long RNAs that give rise to the eutherian-specific clustered 12qF1 microRNAs. Dlk1, Rtl1, and Dio3 are protein-coding genes at 12qF1 that are conserved in preeutherian mammals. ( B ) Schematic of the vertebrate LMO4 3′ UTR depicts that the proximal portion (gray) is well conserved among characterized vertebrate LMO4 mRNAs, whereas the distal portion of the eutherian LMO4 3′ UTR (black) is absent from all characterized chicken LMO4 mRNAs and all marsupial LMO4 genes. The positions of predicted CSMN-enriched 12qF1 miRNA target sites, concentrated in the distal/eutherian portion of the LMO4 3′ UTR, are indicated by colored bars. Multiple sequence alignments illustrate that miR-409-3p site 1 is well conserved among vertebrates, whereas site 2 appears to be well conserved only among eutherians. Predicted mRNAs are italicized; characterized mRNAs are not italicized. ( C ) Individual miRNAs repress multiple targets, and clustered miRNAs cooperatively repress shared targets. Six CSMN-enriched 12qF1 cluster miRNAs, in addition to miR-409-3p, are predicted to cooperatively repress LMO4. ( D ) Model depicting deep-layer CPN and eutherian CSMN derived from ancestral preeutherian CSMN via expansion of gene expression to generate CPN as a new projection neuron subtype, and pruning of this expansion in eutherian CSMN via miRNA-mediated repression of gene expression.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: An evolutionarily acquired microRNA shapes development of mammalian cortical projections

    doi: 10.1073/pnas.2006700117

    Figure Lengend Snippet: CSMN-enriched miRNAs are encoded at a genomic cluster that coevolved with motor cortex and corpus callosum. ( A ) Schematic of the mouse 12qF1 locus highlighting miRNAs identified as enriched in CSMN (magenta). Meg3, anti-Rtl1, Rian, and Mirg are incompletely characterized genes encoding long RNAs that give rise to the eutherian-specific clustered 12qF1 microRNAs. Dlk1, Rtl1, and Dio3 are protein-coding genes at 12qF1 that are conserved in preeutherian mammals. ( B ) Schematic of the vertebrate LMO4 3′ UTR depicts that the proximal portion (gray) is well conserved among characterized vertebrate LMO4 mRNAs, whereas the distal portion of the eutherian LMO4 3′ UTR (black) is absent from all characterized chicken LMO4 mRNAs and all marsupial LMO4 genes. The positions of predicted CSMN-enriched 12qF1 miRNA target sites, concentrated in the distal/eutherian portion of the LMO4 3′ UTR, are indicated by colored bars. Multiple sequence alignments illustrate that miR-409-3p site 1 is well conserved among vertebrates, whereas site 2 appears to be well conserved only among eutherians. Predicted mRNAs are italicized; characterized mRNAs are not italicized. ( C ) Individual miRNAs repress multiple targets, and clustered miRNAs cooperatively repress shared targets. Six CSMN-enriched 12qF1 cluster miRNAs, in addition to miR-409-3p, are predicted to cooperatively repress LMO4. ( D ) Model depicting deep-layer CPN and eutherian CSMN derived from ancestral preeutherian CSMN via expansion of gene expression to generate CPN as a new projection neuron subtype, and pruning of this expansion in eutherian CSMN via miRNA-mediated repression of gene expression.

    Article Snippet: Coverslips were blocked with PBS containing 0.1% Triton X-100, 2% sheep serum, and 1% bovine serum albumin, and cells were then incubated with primary antibodies against cell-specific markers: anti-CTIP2 (Abcam, rat monoclonal, dilution 1:500), and/or anti-SATB2 (Abcam, rabbit monoclonal, dilution 1:100), and/or anti-LMO4 (Abcam, rabbit polyclonal, dilution 1:200).

    Techniques: Sequencing, Derivative Assay, Expressing

    LMO4 levels are significantly associated with patient overall survival (OS) in different tumor stages and sizes. (A,B) OS of patients with high or low-LMO4 expression when further divided into tumor-node-metastasis (TNM) I–II and TNM III–IV subgroups; (C,D) OS rates of patients with tumor size ﹤3 cm and tumor size ≥3 cm, respectively.

    Journal: Journal of Thoracic Disease

    Article Title: LMO4 is a prognostic marker involved in cell migration and invasion in non-small-cell lung cancer

    doi: 10.21037/jtd.2016.12.22

    Figure Lengend Snippet: LMO4 levels are significantly associated with patient overall survival (OS) in different tumor stages and sizes. (A,B) OS of patients with high or low-LMO4 expression when further divided into tumor-node-metastasis (TNM) I–II and TNM III–IV subgroups; (C,D) OS rates of patients with tumor size ﹤3 cm and tumor size ≥3 cm, respectively.

    Article Snippet: The samples were then incubated in a 37 °C warm box for 30 min. Rabbit anti-human LMO4 antibody (1:200 dilution; Abcam) was added and the samples were incubated overnight at 4 °C.

    Techniques: Expressing

    LMO4 levels are significantly associated with patient overall survival (OS) in non-small-cell lung cancer (NSCLC). (A) Immunohistochemical staining of LMO4 in NSCLC tissues and normal tissues (magnification, ×400 for all images); (B) the expression levels of LMO4 in NSCLC tissues and normal tissues as detected by q-RT-PCR. *P

    Journal: Journal of Thoracic Disease

    Article Title: LMO4 is a prognostic marker involved in cell migration and invasion in non-small-cell lung cancer

    doi: 10.21037/jtd.2016.12.22

    Figure Lengend Snippet: LMO4 levels are significantly associated with patient overall survival (OS) in non-small-cell lung cancer (NSCLC). (A) Immunohistochemical staining of LMO4 in NSCLC tissues and normal tissues (magnification, ×400 for all images); (B) the expression levels of LMO4 in NSCLC tissues and normal tissues as detected by q-RT-PCR. *P

    Article Snippet: The samples were then incubated in a 37 °C warm box for 30 min. Rabbit anti-human LMO4 antibody (1:200 dilution; Abcam) was added and the samples were incubated overnight at 4 °C.

    Techniques: Immunohistochemistry, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction