rabbit anti laminin  (Novus Biologicals)

Journal: Journal of Neuroinflammation

Article Title: Carbon monoxide-releasing molecule-3 protects against ischemic stroke by suppressing neuroinflammation and alleviating blood-brain barrier disruption

doi: 10.1186/s12974-018-1226-1

Figure Lengend Snippet: CORM-3 increases the expression of ZO-1 and laminin on day 3 after cerebral infarction. a Representative images show double immunofluorescence staining of CD31/ZO-1-positive cells in peri-infarction zones. Insets show high-magnification images of the boxed areas. Scale bar = 50 μm. b Quantification of ZO-1-positive area/CD31-positive area in peri-infarction zones (* p

Article Snippet: After being washed in PBS, the sections were incubated in PBST (0.3% Triton X-100 in PBS), blocked in 1% bovine serum albumin (BSA)/PBST, and subsequently incubated with the following primary antibodies overnight at 4 °C: goat anti-Iba1 (1:1000, Abcam, Cambridge, MA, USA), rat anti-CD31 (1:500, Abcam), rabbit anti-ZO-1 (1:150, Proteintech, Sanying Biotechnology, Wuhan, China), rabbit anti-laminin (1:1000, Novus Biologicals, Littleton, CO, USA), rat anti-PDGFRβ (1:1000, Abcam), rabbit anti-MMP-9 (1:1000, Abcam).

Techniques: Expressing, Double Immunofluorescence Staining

Journal: Scientific Reports

Article Title: Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix

doi: 10.1038/s41598-020-78477-x

Figure Lengend Snippet: Non-collagenous structures assessment. Representative images of rat dental pulp tissues stained with alcian blue, labelled with fibronectin, laminin and α-gal antibodies. Control tissues reveal a rich glycosaminoglycans rich matrix and a positive immunoreactivity to fibronectin, laminin proteins and α-gal. Following decellularisation, preservation of glycosaminoglycans, fibronectin and laminin proteins with negative α-gal staining was evident. α-gal alpha-1,3 galactose. Scale bars are at 50 µm.

Article Snippet: Following antigen retrieval, slides were then incubated at room temperature for 1 h with the following primary antibodies; mouse monoclonal antibodies against collagen type I (1:100, ab90395, Abcam, Cambridge, United Kingdom), collagen type III (1:200, ab6310, Abcam), fibronectin (1:50, ab6328, Abcam,), Alpha-1,3 Galactose (α-gal, 1:200, ALX-801–090, Enzo Life Sciences, Inc., Lausen, Switzerland), major histocompatibility class II RT1B (1:200, Bio-Rad Laboratories Ltd, Hertfordshire, United Kingdom), and rabbit polyclonal laminin antibody (1:400, NB300-144, Novus Biologicals, Abingdon, United Kingdom) were used to assess collagen matrix, xenoepitope expression, specific cellular and basement proteins components.

Techniques: Staining, Preserving

Journal: PLoS ONE

Article Title: Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS

doi: 10.1371/journal.pone.0040443

Figure Lengend Snippet: mMCAM colocalizes with laminin 411 on the choroid plexus, and shows no specific binding to tissues from LAMA4−/− mice. (A) Staining of healthy mouse choroid plexus with anti-laminin, anti-laminin α4 and mMCAM-Fc (B) Staining of healthy brain tissues from LAMA4−/− mice with anti-laminin and mMCAM-Fc.

Article Snippet: To further explore the functional interaction between MCAM and laminin 411 we examined the ability of mMCAM-expressed cells to capture recombinant laminin 411 from solution. mMCAM-transfected CHO cells were incubated with laminin 411 washed and then exposed to a pan laminin antibody to detect binding of laminin to the surface of the cells.

Techniques: Binding Assay, Mouse Assay, Staining

Journal: PLoS ONE

Article Title: Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS

doi: 10.1371/journal.pone.0040443

Figure Lengend Snippet: Laminin 411 functions as a ligand for MCAM. (A) CHO cells transfected with hMCAM were incubated with recombinant laminin 411. Laminin binding to the surface of the cells was detected with an anti-laminin antibody (left panel), while no binding was detected to CHO cells lacking MCAM expression (center panel). Recombinant laminin 511 did not bind to MCAM expressing CHO cells, and binding of laminin 411 was specifically inhibited by preincubation of the cells with anti-hMCAM (clone 17, right panel). (B) Human CD4+CD45RO+ memory T cells were purified and incubated with TCR stimulation in the presence of TGFβ and IL1β to induce hMCAM expression (approximately 50% expressed hMCAM, similar to Figure 2A ). Cells were incubated in plates coated with either laminin 411 or laminin 511 in the presence of either hMCAM-Fc or anti-hMCAM and specific binding of the cells to the laminin was determined. * indicates p

Article Snippet: To further explore the functional interaction between MCAM and laminin 411 we examined the ability of mMCAM-expressed cells to capture recombinant laminin 411 from solution. mMCAM-transfected CHO cells were incubated with laminin 411 washed and then exposed to a pan laminin antibody to detect binding of laminin to the surface of the cells.

Techniques: Transfection, Incubation, Recombinant, Binding Assay, Expressing, Purification

Journal: PLoS ONE

Article Title: Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS

doi: 10.1371/journal.pone.0040443

Figure Lengend Snippet: hMCAM binds to a ligand in the ECM with identical staining to laminin α4. Calcein labeled, hMCAM expressing MOLT 4 cells were preincubated with either isotype control (A) or anti-hMCAM (clone 17) (B) followed by incubation on tissue sections from healthy mice. After gentle washing of unbound cells, and mounting with DAPI, bound cells were visualized by fluorescent microscopy. Healthy mouse brain sections containing choroid plexus were stained with fluorescently labeled mMCAM-Fc protein and pan-laminin antibody. Staining of mMCAM-Fc was detected on choroid plexus (C) as well as the vasculature throughout the tissues (D). Fluorescently labeled mMCAM-Fc protein was preincubated with either isotype control (E) or anti-mMCAM (clone 15) (F) before addition to tissue sections of healthy mouse brain. Healthy mouse tissues were stained with fluorescently labeled mMCAM-Fc and CD31 (G) anti-mMCAM and pan laminin (H) or anti-mMCAM alone (I). Tissues from mice with active EAE were stained with fluorescently labeled mMCAM-Fc and pan-laminin (J) or mMCAM-Fc and an antibody specific to the α4 chain of laminin (K).

Article Snippet: To further explore the functional interaction between MCAM and laminin 411 we examined the ability of mMCAM-expressed cells to capture recombinant laminin 411 from solution. mMCAM-transfected CHO cells were incubated with laminin 411 washed and then exposed to a pan laminin antibody to detect binding of laminin to the surface of the cells.

Techniques: Staining, Labeling, Expressing, Incubation, Mouse Assay, Microscopy

Journal: Biomaterials

Article Title: Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity

doi: 10.1016/j.biomaterials.2012.08.021

Figure Lengend Snippet: Laminin-111-modified nanofibers increase ductal (SIMS) and acinar (SMGC1O) cell proliferation without affecting cell viability. Total cell number graphs showing increased (A) SIMS cell and (B) SMGC10 cell proliferation for cells cultured on laminin-111-modified

Article Snippet: Primary antibodies used included mouse anti-ZO-1 (Invitrogen Cat No. 339100), mouse anti-occludin (Invitrogen Cat No. 33-1500), rabbit anti-aquaporin 5 (Alamone labs Cat No. (AQP5-005), rat anti-CD49f (Integrin α6) (BD Pharmingen Cat No. 555734), and rabbit anti-laminin-111 (Novus Biologicals 300-144).

Techniques: Modification, Cell Culture

Journal: Biomaterials

Article Title: Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity

doi: 10.1016/j.biomaterials.2012.08.021

Figure Lengend Snippet: Laminin-111 modification of nanofibers enhances polarity of SMGC10 salivary gland acinar cells. Cells were immunostained for (A) ZO-1 and (B) occludin, as indicators of apicobasal polarity. Single apical and basal confocal images and XZ projections indicate

Article Snippet: Primary antibodies used included mouse anti-ZO-1 (Invitrogen Cat No. 339100), mouse anti-occludin (Invitrogen Cat No. 33-1500), rabbit anti-aquaporin 5 (Alamone labs Cat No. (AQP5-005), rat anti-CD49f (Integrin α6) (BD Pharmingen Cat No. 555734), and rabbit anti-laminin-111 (Novus Biologicals 300-144).

Techniques: Modification

Journal: Biomaterials

Article Title: Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity

doi: 10.1016/j.biomaterials.2012.08.021

Figure Lengend Snippet: Bifunctional nanofibers induce an intermediate polarity phenotype in SIMS ductal cells. SIMS cells were cultured for 48 hours on nanofibers, chitosan-coated fibers, laminin-111-coated fibers, or chitosan and laminin-111- coated fibers. Cells were immunostained

Article Snippet: Primary antibodies used included mouse anti-ZO-1 (Invitrogen Cat No. 339100), mouse anti-occludin (Invitrogen Cat No. 33-1500), rabbit anti-aquaporin 5 (Alamone labs Cat No. (AQP5-005), rat anti-CD49f (Integrin α6) (BD Pharmingen Cat No. 555734), and rabbit anti-laminin-111 (Novus Biologicals 300-144).

Techniques: Cell Culture

Journal: Biomaterials

Article Title: Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity

doi: 10.1016/j.biomaterials.2012.08.021

Figure Lengend Snippet: PLGA fiber scaffolds modified with chitosan and/or laminin-111 post-electrospinning. Fluorescence microscopy of unmodified, FITC-chitosan modified, laminin-111-modified, and chitosan- and laminin-111-modified PLGA fiber scaffolds prepared with the post-electrospinning

Article Snippet: Primary antibodies used included mouse anti-ZO-1 (Invitrogen Cat No. 339100), mouse anti-occludin (Invitrogen Cat No. 33-1500), rabbit anti-aquaporin 5 (Alamone labs Cat No. (AQP5-005), rat anti-CD49f (Integrin α6) (BD Pharmingen Cat No. 555734), and rabbit anti-laminin-111 (Novus Biologicals 300-144).

Techniques: Modification, Fluorescence, Microscopy

Journal: Biomaterials

Article Title: Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity

doi: 10.1016/j.biomaterials.2012.08.021

Figure Lengend Snippet: Laminin-111 modification of nanofibers enhances polarity of SIMS cells. Cells were immunostained for (A) ZO-1 and (B) occludin as indicators of apicobasal polarity. Single apical and basal confocal images and XZ projections indicate that both ZO-1 and

Article Snippet: Primary antibodies used included mouse anti-ZO-1 (Invitrogen Cat No. 339100), mouse anti-occludin (Invitrogen Cat No. 33-1500), rabbit anti-aquaporin 5 (Alamone labs Cat No. (AQP5-005), rat anti-CD49f (Integrin α6) (BD Pharmingen Cat No. 555734), and rabbit anti-laminin-111 (Novus Biologicals 300-144).

Techniques: Modification