rabbit polyclonal antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal antibody
    Immunoprecipitation of DPPX In cultures of dissociated rat hippocampal neurons, patients’ antibodies showed intense reactivity with the neuronal cell surface (A), bar = 10 μm. Immunoprecipitation of the antigen with serum of the index case is shown in B, where the precipitated proteins were run in a gel and subsequently stained with EZblue. Note that patient’s antibodies precipitated a protein (band close to 102 kDa in lane P), which was excised from the gel and analyzed by mass spectrometry, demonstrating sequences of DPPX. Lane N is the precipitate obtained from control serum. Immunoblot of these proteins with a rabbit <t>polyclonal</t> antibody against DPPX (1:1000, developed by BR) confirmed that the band corresponded to DPPX (C).
    Rabbit Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibody - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "Encephalitis and antibodies to DPPX, a subunit of Kv4.2 potassium channels"

    Article Title: Encephalitis and antibodies to DPPX, a subunit of Kv4.2 potassium channels

    Journal: Annals of neurology

    doi: 10.1002/ana.23756

    Immunoprecipitation of DPPX In cultures of dissociated rat hippocampal neurons, patients’ antibodies showed intense reactivity with the neuronal cell surface (A), bar = 10 μm. Immunoprecipitation of the antigen with serum of the index case is shown in B, where the precipitated proteins were run in a gel and subsequently stained with EZblue. Note that patient’s antibodies precipitated a protein (band close to 102 kDa in lane P), which was excised from the gel and analyzed by mass spectrometry, demonstrating sequences of DPPX. Lane N is the precipitate obtained from control serum. Immunoblot of these proteins with a rabbit polyclonal antibody against DPPX (1:1000, developed by BR) confirmed that the band corresponded to DPPX (C).
    Figure Legend Snippet: Immunoprecipitation of DPPX In cultures of dissociated rat hippocampal neurons, patients’ antibodies showed intense reactivity with the neuronal cell surface (A), bar = 10 μm. Immunoprecipitation of the antigen with serum of the index case is shown in B, where the precipitated proteins were run in a gel and subsequently stained with EZblue. Note that patient’s antibodies precipitated a protein (band close to 102 kDa in lane P), which was excised from the gel and analyzed by mass spectrometry, demonstrating sequences of DPPX. Lane N is the precipitate obtained from control serum. Immunoblot of these proteins with a rabbit polyclonal antibody against DPPX (1:1000, developed by BR) confirmed that the band corresponded to DPPX (C).

    Techniques Used: Immunoprecipitation, Staining, Mass Spectrometry

    Expression of DPPX in myenteric plexus Transverse section of small bowel of rat showing the longitudinal muscular layer (LM), circular muscular layer (CM), submucosal layer (SM), and glans (G). The myenteric plexus (Plex) is revealed as clusters of large neurons between the two muscular layers. In the 3 panels (A–C) the nuclei of the neurons (red) was labeled with anti-Hu (a highly specific neuronal marker). Panel A, shows in green the DPPX immunostaining using a rabbit polyclonal antibody (1:1000, developed by BD); panel B shows the DPPX reactivity of serum from one of the patients with encephalitis, and panel C shows the lack of reactivity of serum from a healthy subject. Note that DPPX is predominantly expressed in the cytoplasm-membrane of the large clustered neurons of the myenteric plexus, and is also detected in a fine longitudinal pattern in CM and SM where the submucosal plexus is located. Bar = 20μm.
    Figure Legend Snippet: Expression of DPPX in myenteric plexus Transverse section of small bowel of rat showing the longitudinal muscular layer (LM), circular muscular layer (CM), submucosal layer (SM), and glans (G). The myenteric plexus (Plex) is revealed as clusters of large neurons between the two muscular layers. In the 3 panels (A–C) the nuclei of the neurons (red) was labeled with anti-Hu (a highly specific neuronal marker). Panel A, shows in green the DPPX immunostaining using a rabbit polyclonal antibody (1:1000, developed by BD); panel B shows the DPPX reactivity of serum from one of the patients with encephalitis, and panel C shows the lack of reactivity of serum from a healthy subject. Note that DPPX is predominantly expressed in the cytoplasm-membrane of the large clustered neurons of the myenteric plexus, and is also detected in a fine longitudinal pattern in CM and SM where the submucosal plexus is located. Bar = 20μm.

    Techniques Used: Expressing, Labeling, Marker, Immunostaining

    2) Product Images from "Encephalitis and antibodies to DPPX, a subunit of Kv4.2 potassium channels"

    Article Title: Encephalitis and antibodies to DPPX, a subunit of Kv4.2 potassium channels

    Journal: Annals of neurology

    doi: 10.1002/ana.23756

    Immunoprecipitation of DPPX In cultures of dissociated rat hippocampal neurons, patients’ antibodies showed intense reactivity with the neuronal cell surface (A), bar = 10 μm. Immunoprecipitation of the antigen with serum of the index case is shown in B, where the precipitated proteins were run in a gel and subsequently stained with EZblue. Note that patient’s antibodies precipitated a protein (band close to 102 kDa in lane P), which was excised from the gel and analyzed by mass spectrometry, demonstrating sequences of DPPX. Lane N is the precipitate obtained from control serum. Immunoblot of these proteins with a rabbit polyclonal antibody against DPPX (1:1000, developed by BR) confirmed that the band corresponded to DPPX (C).
    Figure Legend Snippet: Immunoprecipitation of DPPX In cultures of dissociated rat hippocampal neurons, patients’ antibodies showed intense reactivity with the neuronal cell surface (A), bar = 10 μm. Immunoprecipitation of the antigen with serum of the index case is shown in B, where the precipitated proteins were run in a gel and subsequently stained with EZblue. Note that patient’s antibodies precipitated a protein (band close to 102 kDa in lane P), which was excised from the gel and analyzed by mass spectrometry, demonstrating sequences of DPPX. Lane N is the precipitate obtained from control serum. Immunoblot of these proteins with a rabbit polyclonal antibody against DPPX (1:1000, developed by BR) confirmed that the band corresponded to DPPX (C).

    Techniques Used: Immunoprecipitation, Staining, Mass Spectrometry

    Expression of DPPX in myenteric plexus Transverse section of small bowel of rat showing the longitudinal muscular layer (LM), circular muscular layer (CM), submucosal layer (SM), and glans (G). The myenteric plexus (Plex) is revealed as clusters of large neurons between the two muscular layers. In the 3 panels (A–C) the nuclei of the neurons (red) was labeled with anti-Hu (a highly specific neuronal marker). Panel A, shows in green the DPPX immunostaining using a rabbit polyclonal antibody (1:1000, developed by BD); panel B shows the DPPX reactivity of serum from one of the patients with encephalitis, and panel C shows the lack of reactivity of serum from a healthy subject. Note that DPPX is predominantly expressed in the cytoplasm-membrane of the large clustered neurons of the myenteric plexus, and is also detected in a fine longitudinal pattern in CM and SM where the submucosal plexus is located. Bar = 20μm.
    Figure Legend Snippet: Expression of DPPX in myenteric plexus Transverse section of small bowel of rat showing the longitudinal muscular layer (LM), circular muscular layer (CM), submucosal layer (SM), and glans (G). The myenteric plexus (Plex) is revealed as clusters of large neurons between the two muscular layers. In the 3 panels (A–C) the nuclei of the neurons (red) was labeled with anti-Hu (a highly specific neuronal marker). Panel A, shows in green the DPPX immunostaining using a rabbit polyclonal antibody (1:1000, developed by BD); panel B shows the DPPX reactivity of serum from one of the patients with encephalitis, and panel C shows the lack of reactivity of serum from a healthy subject. Note that DPPX is predominantly expressed in the cytoplasm-membrane of the large clustered neurons of the myenteric plexus, and is also detected in a fine longitudinal pattern in CM and SM where the submucosal plexus is located. Bar = 20μm.

    Techniques Used: Expressing, Labeling, Marker, Immunostaining

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    Alomone Labs kv4 2
    Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, <t>Kv4.2,</t> Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P
    Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv4 2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kv4 2 - by Bioz Stars, 2022-09
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    94
    Alomone Labs rabbit anti kv4 2
    VE and LOC neurons express Kv4.3 and Kv 4.2 subunits in the mouse. Adult mouse immunofluorescently labeled against ChAT (red) and <t>Kv4.2</t> or Kv4.3 α-subunits (green) using shock-frozen tissue. The ChAT labeling overlaps with the Kv4.3 labeling in the mouse VE neurons (A–C). Likewise, both the ChAT-positive LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) show Kv4.3 expression in the mouse (D–F). In a similar fashion, both the VE neurons (G–I) and the LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) (J–L) are immunolabeled against Kv4.2, which indicate that the Kv4 channels are hetero-meric in these auditory brainstem nuclei. Scale bars: 100 µm.
    Rabbit Anti Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv4 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv4 2 - by Bioz Stars, 2022-09
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    Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, Kv4.2, Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P

    Journal: Nature neuroscience

    Article Title: G9a Is Essential for Epigenetic Silencing of K+ Channel Genes in Acute-to-Chronic Pain Transition

    doi: 10.1038/nn.4165

    Figure Lengend Snippet: Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, Kv4.2, Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P

    Article Snippet: The membranes were treated with 5% bovine serum albumin in Tris buffer containing Tween 20 (TNT) for 2 h and then incubated with one of the following primary antibodies overnight at 4°C: Kv1.4 (catalog #05-409, Upstate Biotechnology), Kv4.2 (catalog #APC-023, Alomone Labs), Kv7.2 (catalog #APC-050, Alomone Labs), BKα1 (catalog #APC-021, Alomone Labs), acetyl-H3 (catalog #06-599, Millipore), H3K9ac (catalog #39917, Active Motif), H3K27me3 (catalog #9733, Cell Signaling Technology), histone H3 (catalog #9715, Cell Signaling Technology), H3K9me2 (catalog #ab1220, Abcam), G9a (catalog #09-071, Millipore), HDAC1 (catalog #IMG-337, Imgenex), and HDAC2, HDAC4, HDAC5 and EZH2 (catalog #2540, #2072, #2082 and #5246, respectively; Cell Signaling Technology).

    Techniques: Inhibition, Activity Assay, Expressing, Injection, MANN-WHITNEY

    Blockade of phosphorylation at the PKC sites increases the surface expression of Kv4.2 channels in COS-7 cells

    Journal: The Biochemical journal

    Article Title: Kv4.2 is a locus for PKC and ERK/MAPK cross-talk

    doi: 10.1042/BJ20081213

    Figure Lengend Snippet: Blockade of phosphorylation at the PKC sites increases the surface expression of Kv4.2 channels in COS-7 cells

    Article Snippet: Briefly, after fixing cells with 4% paraformaldehyde for 30 min, and incubation in 0.3% Triton X-100 in PBS for 20 min at room temperature, the cells were blocked by 10% fetal bovine serum in PBS for 60 min at room temperature, and then incubated for 60 min at room temperature or overnight at 4°C with primary antibodies: polyclonal Kv4.2 antibody from Alomone Labs (Jerusalem, Israel) generated against residues 454-469 ( SNQLQSSEDEPAFVSK) on the C-terminal or a monoclonal Kv4.2 antibody against an ectodomain [ ].

    Techniques: Expressing

    PKC phosphorylates the Kv4.2 C-terminal but not the N-terminal cytoplasmic domains in vitro

    Journal: The Biochemical journal

    Article Title: Kv4.2 is a locus for PKC and ERK/MAPK cross-talk

    doi: 10.1042/BJ20081213

    Figure Lengend Snippet: PKC phosphorylates the Kv4.2 C-terminal but not the N-terminal cytoplasmic domains in vitro

    Article Snippet: Briefly, after fixing cells with 4% paraformaldehyde for 30 min, and incubation in 0.3% Triton X-100 in PBS for 20 min at room temperature, the cells were blocked by 10% fetal bovine serum in PBS for 60 min at room temperature, and then incubated for 60 min at room temperature or overnight at 4°C with primary antibodies: polyclonal Kv4.2 antibody from Alomone Labs (Jerusalem, Israel) generated against residues 454-469 ( SNQLQSSEDEPAFVSK) on the C-terminal or a monoclonal Kv4.2 antibody against an ectodomain [ ].

    Techniques: In Vitro

    Generation and characterization of the Kv4.2 phospho-specific antibodies for the Ser447 and Ser537 site

    Journal: The Biochemical journal

    Article Title: Kv4.2 is a locus for PKC and ERK/MAPK cross-talk

    doi: 10.1042/BJ20081213

    Figure Lengend Snippet: Generation and characterization of the Kv4.2 phospho-specific antibodies for the Ser447 and Ser537 site

    Article Snippet: Briefly, after fixing cells with 4% paraformaldehyde for 30 min, and incubation in 0.3% Triton X-100 in PBS for 20 min at room temperature, the cells were blocked by 10% fetal bovine serum in PBS for 60 min at room temperature, and then incubated for 60 min at room temperature or overnight at 4°C with primary antibodies: polyclonal Kv4.2 antibody from Alomone Labs (Jerusalem, Israel) generated against residues 454-469 ( SNQLQSSEDEPAFVSK) on the C-terminal or a monoclonal Kv4.2 antibody against an ectodomain [ ].

    Techniques:

    PKC phosphorylation of the Kv4.2 C-terminal augments ERK phosphorylation of the Kv4.2 C-terminal in vitro

    Journal: The Biochemical journal

    Article Title: Kv4.2 is a locus for PKC and ERK/MAPK cross-talk

    doi: 10.1042/BJ20081213

    Figure Lengend Snippet: PKC phosphorylation of the Kv4.2 C-terminal augments ERK phosphorylation of the Kv4.2 C-terminal in vitro

    Article Snippet: Briefly, after fixing cells with 4% paraformaldehyde for 30 min, and incubation in 0.3% Triton X-100 in PBS for 20 min at room temperature, the cells were blocked by 10% fetal bovine serum in PBS for 60 min at room temperature, and then incubated for 60 min at room temperature or overnight at 4°C with primary antibodies: polyclonal Kv4.2 antibody from Alomone Labs (Jerusalem, Israel) generated against residues 454-469 ( SNQLQSSEDEPAFVSK) on the C-terminal or a monoclonal Kv4.2 antibody against an ectodomain [ ].

    Techniques: In Vitro

    Functional characterization of PKC sites within Kv4.2

    Journal: The Biochemical journal

    Article Title: Kv4.2 is a locus for PKC and ERK/MAPK cross-talk

    doi: 10.1042/BJ20081213

    Figure Lengend Snippet: Functional characterization of PKC sites within Kv4.2

    Article Snippet: Briefly, after fixing cells with 4% paraformaldehyde for 30 min, and incubation in 0.3% Triton X-100 in PBS for 20 min at room temperature, the cells were blocked by 10% fetal bovine serum in PBS for 60 min at room temperature, and then incubated for 60 min at room temperature or overnight at 4°C with primary antibodies: polyclonal Kv4.2 antibody from Alomone Labs (Jerusalem, Israel) generated against residues 454-469 ( SNQLQSSEDEPAFVSK) on the C-terminal or a monoclonal Kv4.2 antibody against an ectodomain [ ].

    Techniques: Functional Assay

    Candidate PKC consensus sites within the Kv4.2 channel subunit

    Journal: The Biochemical journal

    Article Title: Kv4.2 is a locus for PKC and ERK/MAPK cross-talk

    doi: 10.1042/BJ20081213

    Figure Lengend Snippet: Candidate PKC consensus sites within the Kv4.2 channel subunit

    Article Snippet: Briefly, after fixing cells with 4% paraformaldehyde for 30 min, and incubation in 0.3% Triton X-100 in PBS for 20 min at room temperature, the cells were blocked by 10% fetal bovine serum in PBS for 60 min at room temperature, and then incubated for 60 min at room temperature or overnight at 4°C with primary antibodies: polyclonal Kv4.2 antibody from Alomone Labs (Jerusalem, Israel) generated against residues 454-469 ( SNQLQSSEDEPAFVSK) on the C-terminal or a monoclonal Kv4.2 antibody against an ectodomain [ ].

    Techniques:

    Expression of A-type K + channels and their contribution to the PE-induced spontaneous firing of the DRN 5-HT neurons. (A) Expression of A-type K + channel-related subfamily members assessed using single-cell PCR analysis in the DRN neurons. (i) Representative image of single-cell PCR products showing the presence of different Kv subunits. (ii) Proportion of Kv1.4, 3.3, 3.4, and Kv4s-positive neurons in the TPH-positive neurons ( n = 20). (B) (i) Confocal images of Kv4.2 and 4.3 protein expression in slice of the DRN, assessed using immunofluorescence methods. Scale bar = 50 μm. (ii) Proportion of Kv4.2 and 4.3-positive neurons in the TPH-positive neurons ( n = 200). (C) PE potently inhibited A-type currents recorded using whole-cell patch clamp in the DRN 5-HT neurons. (i) Recording protocol used and the typical current traces recorded; the latter were from –20 mV. The current amplitude at the beginning of the –20 mV step was measured (dotted square, enlarged in inset). (ii) Time course for current amplitudes measured in (i) . (iii) Summarized data for experiments shown in (i,ii) . Paired t -test, ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Potassium Channel Conductance Is Involved in Phenylephrine-Induced Spontaneous Firing of Serotonergic Neurons in the Dorsal Raphe Nucleus

    doi: 10.3389/fncel.2022.891912

    Figure Lengend Snippet: Expression of A-type K + channels and their contribution to the PE-induced spontaneous firing of the DRN 5-HT neurons. (A) Expression of A-type K + channel-related subfamily members assessed using single-cell PCR analysis in the DRN neurons. (i) Representative image of single-cell PCR products showing the presence of different Kv subunits. (ii) Proportion of Kv1.4, 3.3, 3.4, and Kv4s-positive neurons in the TPH-positive neurons ( n = 20). (B) (i) Confocal images of Kv4.2 and 4.3 protein expression in slice of the DRN, assessed using immunofluorescence methods. Scale bar = 50 μm. (ii) Proportion of Kv4.2 and 4.3-positive neurons in the TPH-positive neurons ( n = 200). (C) PE potently inhibited A-type currents recorded using whole-cell patch clamp in the DRN 5-HT neurons. (i) Recording protocol used and the typical current traces recorded; the latter were from –20 mV. The current amplitude at the beginning of the –20 mV step was measured (dotted square, enlarged in inset). (ii) Time course for current amplitudes measured in (i) . (iii) Summarized data for experiments shown in (i,ii) . Paired t -test, ** p

    Article Snippet: Commercial antibodies used were anti-TPH (1:400, mouse, sigma, T0678, RRID:AB_261587 ), anti-Kv4.2 (1:400, rabbit, AlomoneLabs, APC-023, RRID:AB_2040176 ), anti-Kv4.3 (1:400, rabbit, AlomoneLabs, APC-017, RRID:AB_2040178 ), anti-SK2 (1:200, rabbit, Bioss, DF13499, RRID:AB_2846518 ), anti-SK3 (1:200, rabbit, Proteintech, 17188-1-AP), anti-KCNQ2 (1:200, goat, Santa Cruz, sc-7793, RRID:AB_2296585 ), anti-KCNQ3 (1:200, goat, Santa Cruz, sc-7794, RRID:AB_2131714 ), and anti-KCNQ4 (1:200, rabbit, AlomoneLabs, APC-164, RRID:AB_2341042 ).

    Techniques: Expressing, Polymerase Chain Reaction, Immunofluorescence, Patch Clamp

    VE and LOC neurons express Kv4.3 and Kv 4.2 subunits in the mouse. Adult mouse immunofluorescently labeled against ChAT (red) and Kv4.2 or Kv4.3 α-subunits (green) using shock-frozen tissue. The ChAT labeling overlaps with the Kv4.3 labeling in the mouse VE neurons (A–C). Likewise, both the ChAT-positive LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) show Kv4.3 expression in the mouse (D–F). In a similar fashion, both the VE neurons (G–I) and the LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) (J–L) are immunolabeled against Kv4.2, which indicate that the Kv4 channels are hetero-meric in these auditory brainstem nuclei. Scale bars: 100 µm.

    Journal: PLoS ONE

    Article Title: Physiological Characterization of Vestibular Efferent Brainstem Neurons Using a Transgenic Mouse Model

    doi: 10.1371/journal.pone.0098277

    Figure Lengend Snippet: VE and LOC neurons express Kv4.3 and Kv 4.2 subunits in the mouse. Adult mouse immunofluorescently labeled against ChAT (red) and Kv4.2 or Kv4.3 α-subunits (green) using shock-frozen tissue. The ChAT labeling overlaps with the Kv4.3 labeling in the mouse VE neurons (A–C). Likewise, both the ChAT-positive LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) show Kv4.3 expression in the mouse (D–F). In a similar fashion, both the VE neurons (G–I) and the LOC efferent cells (arrows) and the principal cells of the lateral superior olive (arrowheads) (J–L) are immunolabeled against Kv4.2, which indicate that the Kv4 channels are hetero-meric in these auditory brainstem nuclei. Scale bars: 100 µm.

    Article Snippet: Primary antibodies used were AlexaFluor488-conjugated rabbit anti-GFP 1∶500 (Invitrogen, Corp., Carlsbad, CA), goat anti-ChAT 1∶100 (Millipore, Corp., Temecula, CA), rabbit anti-Kv4.2 1∶100 and rabbit anti-Kv4.3 1∶100 (Alomone Labs, Ltd., Jerusalem, Israel).

    Techniques: Labeling, Expressing, Immunolabeling