rabbit anti kv 4 1  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti kv 4 1
    Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.
    Rabbit Anti Kv 4 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv 4 1/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv 4 1 - by Bioz Stars, 2022-09
    85/100 stars

    Images

    1) Product Images from "Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats"

    Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/j.1755-5949.2010.00133.x

    Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.
    Figure Legend Snippet: Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.

    Techniques Used: Immunofluorescence, Staining, Labeling

    Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia.  a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp,  b ), Kv 4.1 (467 bp,  c ), Kv 4.2 (110 bp,  d ), Kv 4.3 (400 bp,  e ), and GAPDH (418 bp,  f ). Negative control (no reverse transcription,  g ).
    Figure Legend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

    Techniques Used: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

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  • 94
    Alomone Labs rabbit anti kv 4 3
    Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia.  a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp,  b ), Kv 4.1 (467 bp,  c ), Kv 4.2 (110 bp,  d ), Kv 4.3 (400 bp,  e ), and GAPDH (418 bp,  f ). Negative control (no reverse transcription,  g ).
    Rabbit Anti Kv 4 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv 4 3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv 4 3 - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    85
    Alomone Labs rabbit anti kv 4 1
    Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.
    Rabbit Anti Kv 4 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv 4 1/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv 4 1 - by Bioz Stars, 2022-09
    85/100 stars
      Buy from Supplier

    94
    Alomone Labs kcnq1 kv 7 1
    The effect of dextromethorphan on K V <t>7.1</t> protein expression in mouse hearts. ( a ) Representative immunoblots of K V 7.1, β-tubulin and Gapdh protein using mouse heart lysates from TS (non-treated and Dxm-treated) mice and the control littermates (Ctrl) at day 11 (each, n =3, using the same samples with the global proteomics shown in Figure 5e ). #, bands indicate putative degradation of K V 7.1 in TS mouse hearts. ( b-c ) Quantification of β-tubulin ( b ) and <t>Kcnq1/K</t> V 7.1 protein expression ( c , normalized to Gapdh) in TS (non-treated and Dxm-treated) and Ctrl mouse hearts ( n =3/group). Altered β-tubulin expression has been reported previously in animal model of myocardial infarction and Gapdh was recommended as a more reliable loading control for Western blot analyses 60 . ( d ) Kcnq1/ K V 7.1 mRNA expression (normalized to Gapdh) in TS (non-treated and Dxm-treated) and Ctrl mouse hearts ( n =3/group). All data are mean ± s.d. One-way ANOVA with Tukey’s multiple comparisons was used. ** P
    Kcnq1 Kv 7 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnq1 kv 7 1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kcnq1 kv 7 1 - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia.  a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp,  b ), Kv 4.1 (467 bp,  c ), Kv 4.2 (110 bp,  d ), Kv 4.3 (400 bp,  e ), and GAPDH (418 bp,  f ). Negative control (no reverse transcription,  g ).

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

    doi: 10.1111/j.1755-5949.2010.00133.x

    Figure Lengend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

    Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

    Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

    doi: 10.1111/j.1755-5949.2010.00133.x

    Figure Lengend Snippet: Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.

    Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

    Techniques: Immunofluorescence, Staining, Labeling

    Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia.  a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp,  b ), Kv 4.1 (467 bp,  c ), Kv 4.2 (110 bp,  d ), Kv 4.3 (400 bp,  e ), and GAPDH (418 bp,  f ). Negative control (no reverse transcription,  g ).

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

    doi: 10.1111/j.1755-5949.2010.00133.x

    Figure Lengend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

    Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

    The effect of dextromethorphan on K V 7.1 protein expression in mouse hearts. ( a ) Representative immunoblots of K V 7.1, β-tubulin and Gapdh protein using mouse heart lysates from TS (non-treated and Dxm-treated) mice and the control littermates (Ctrl) at day 11 (each, n =3, using the same samples with the global proteomics shown in Figure 5e ). #, bands indicate putative degradation of K V 7.1 in TS mouse hearts. ( b-c ) Quantification of β-tubulin ( b ) and Kcnq1/K V 7.1 protein expression ( c , normalized to Gapdh) in TS (non-treated and Dxm-treated) and Ctrl mouse hearts ( n =3/group). Altered β-tubulin expression has been reported previously in animal model of myocardial infarction and Gapdh was recommended as a more reliable loading control for Western blot analyses 60 . ( d ) Kcnq1/ K V 7.1 mRNA expression (normalized to Gapdh) in TS (non-treated and Dxm-treated) and Ctrl mouse hearts ( n =3/group). All data are mean ± s.d. One-way ANOVA with Tukey’s multiple comparisons was used. ** P

    Journal: Nature cardiovascular research

    Article Title: Sigma non-opioid receptor 1 is a potential therapeutic target for long QT syndrome

    doi: 10.1038/s44161-021-00016-2

    Figure Lengend Snippet: The effect of dextromethorphan on K V 7.1 protein expression in mouse hearts. ( a ) Representative immunoblots of K V 7.1, β-tubulin and Gapdh protein using mouse heart lysates from TS (non-treated and Dxm-treated) mice and the control littermates (Ctrl) at day 11 (each, n =3, using the same samples with the global proteomics shown in Figure 5e ). #, bands indicate putative degradation of K V 7.1 in TS mouse hearts. ( b-c ) Quantification of β-tubulin ( b ) and Kcnq1/K V 7.1 protein expression ( c , normalized to Gapdh) in TS (non-treated and Dxm-treated) and Ctrl mouse hearts ( n =3/group). Altered β-tubulin expression has been reported previously in animal model of myocardial infarction and Gapdh was recommended as a more reliable loading control for Western blot analyses 60 . ( d ) Kcnq1/ K V 7.1 mRNA expression (normalized to Gapdh) in TS (non-treated and Dxm-treated) and Ctrl mouse hearts ( n =3/group). All data are mean ± s.d. One-way ANOVA with Tukey’s multiple comparisons was used. ** P

    Article Snippet: The samples were next incubated in primary antibody overnight at 4°C (KCNQ1/KV 7.1, Alomone lab, # APC-022, 1:500; SIGMAR1, Santa Cruz, # sc-137075 (mouse), 1:500; SIGMAR1, Abcam, # ab53852 (rabbit), 1:500; KCNH2/hERG, Santa Cruz, # sc-377388, 1:500; CDK5, Abcam, # ab40773, 1:500; CaV1.2, Alomone labs, # ACC-003, 1:100).

    Techniques: Expressing, Western Blot, Mouse Assay, Animal Model

    The effect of dextromethorphan on potassium channels in Timothy syndrome iPSC-derived cardiomyocytes. ( a ) Representative confocal fluorescent images of Timothy syndrome cardiomyocytes without treatment (left, TS) and with dextromethorphan (+Dxm). Scale bar, 5μm. ( b ) Quantification of fraction of green (hERG) over red (WGA) fluorescence signals in TS cardiomyocytes without treatment ( n =38) and with Dxm ( n =31). ( c ) Quantification of KCNH2 transcript in TS cardiomyocytes with and without Dxm ( n =4/group). ( d ) Representative immunoblots of human hERG and GAPDH protein using lysates from non-treated and Dxm-treated TS cardiomyocytes. ( e ) Quantification of hERG protein expressions (normalized to GAPDH) in non-treated and Dxm-treated TS cardiomyocytes ( n =3/group). The trend towards a decreased hERG protein expression in the treated group might be driven by one sample ( # ) with a reduced loading amount compared with others. ( f ) I Ks current steady-state amplitudes were significantly reduced in TS cardiomyocytes ( n =10) compared to isogenic control ( n =10) at 20 and 50 mV steps from −40mV hold. ( g ) Representative traces of I Ks currents (Chromanol 293B-sensitive) in isogenic Ctrl cardiomyocytes (black) and TS cardiomyocytes treated with Dxm (purple), Dxm and NE-100 (green) or without treatment (red, TS). The I Ks traces are shown in Figure 2l . ( h ) Representative confocal fluorescent images of Timothy syndrome cardiomyocyte without treatment (left, TS) and treated with dextromethorphan (+Dxm). Scale bar, 5μm. ( i ) Quantification of fraction of green (K V 7.1) over red (WGA) fluorescence signals in TS cardiomyocytes without treatment ( n =21) and treated with Dxm ( n =31). ( j ) Quantification of KCNQ1 transcripts (isoform 1 and 2) in TS cardiomyocytes without treatment and treated with Dxm ( n =4/group). ( k ) Representative immunoblots of human K V 7.1 and GAPDH protein using lysates from non-treated and Dxm-treated TS cardiomyocytes. ( l-m ) Quantification of K V 7.1 isoform 1 ( l ) and isoform 2 protein expressions ( m , normalized to GAPDH) in non-treated and Dxm-treated TS cardiomyocytes ( n =3/group). All data are mean ± s.d. The treatment of Dxm or NE-100 for all experiments was 5μM, 2hrs. Unpaired two-tailed Student’s t -test was used for b, c, e, i, j, l, m between the groups and used for f at each voltage step. * P

    Journal: Nature cardiovascular research

    Article Title: Sigma non-opioid receptor 1 is a potential therapeutic target for long QT syndrome

    doi: 10.1038/s44161-021-00016-2

    Figure Lengend Snippet: The effect of dextromethorphan on potassium channels in Timothy syndrome iPSC-derived cardiomyocytes. ( a ) Representative confocal fluorescent images of Timothy syndrome cardiomyocytes without treatment (left, TS) and with dextromethorphan (+Dxm). Scale bar, 5μm. ( b ) Quantification of fraction of green (hERG) over red (WGA) fluorescence signals in TS cardiomyocytes without treatment ( n =38) and with Dxm ( n =31). ( c ) Quantification of KCNH2 transcript in TS cardiomyocytes with and without Dxm ( n =4/group). ( d ) Representative immunoblots of human hERG and GAPDH protein using lysates from non-treated and Dxm-treated TS cardiomyocytes. ( e ) Quantification of hERG protein expressions (normalized to GAPDH) in non-treated and Dxm-treated TS cardiomyocytes ( n =3/group). The trend towards a decreased hERG protein expression in the treated group might be driven by one sample ( # ) with a reduced loading amount compared with others. ( f ) I Ks current steady-state amplitudes were significantly reduced in TS cardiomyocytes ( n =10) compared to isogenic control ( n =10) at 20 and 50 mV steps from −40mV hold. ( g ) Representative traces of I Ks currents (Chromanol 293B-sensitive) in isogenic Ctrl cardiomyocytes (black) and TS cardiomyocytes treated with Dxm (purple), Dxm and NE-100 (green) or without treatment (red, TS). The I Ks traces are shown in Figure 2l . ( h ) Representative confocal fluorescent images of Timothy syndrome cardiomyocyte without treatment (left, TS) and treated with dextromethorphan (+Dxm). Scale bar, 5μm. ( i ) Quantification of fraction of green (K V 7.1) over red (WGA) fluorescence signals in TS cardiomyocytes without treatment ( n =21) and treated with Dxm ( n =31). ( j ) Quantification of KCNQ1 transcripts (isoform 1 and 2) in TS cardiomyocytes without treatment and treated with Dxm ( n =4/group). ( k ) Representative immunoblots of human K V 7.1 and GAPDH protein using lysates from non-treated and Dxm-treated TS cardiomyocytes. ( l-m ) Quantification of K V 7.1 isoform 1 ( l ) and isoform 2 protein expressions ( m , normalized to GAPDH) in non-treated and Dxm-treated TS cardiomyocytes ( n =3/group). All data are mean ± s.d. The treatment of Dxm or NE-100 for all experiments was 5μM, 2hrs. Unpaired two-tailed Student’s t -test was used for b, c, e, i, j, l, m between the groups and used for f at each voltage step. * P

    Article Snippet: The samples were next incubated in primary antibody overnight at 4°C (KCNQ1/KV 7.1, Alomone lab, # APC-022, 1:500; SIGMAR1, Santa Cruz, # sc-137075 (mouse), 1:500; SIGMAR1, Abcam, # ab53852 (rabbit), 1:500; KCNH2/hERG, Santa Cruz, # sc-377388, 1:500; CDK5, Abcam, # ab40773, 1:500; CaV1.2, Alomone labs, # ACC-003, 1:100).

    Techniques: Derivative Assay, Whole Genome Amplification, Fluorescence, Western Blot, Expressing, Two Tailed Test