Structured Review

Santa Cruz Biotechnology rabbit anti keap1
Liver expression of Nrf2, <t>Keap1</t> and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.
Rabbit Anti Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti keap1/product/Santa Cruz Biotechnology
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99
rabbit anti keap1 - by Bioz Stars, 2020-03
99/100 stars

Images

1) Product Images from "Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis"

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis

Journal: Scientific Reports

doi: 10.1038/srep44769

Liver expression of Nrf2, Keap1 and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.
Figure Legend Snippet: Liver expression of Nrf2, Keap1 and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.

Techniques Used: Expressing, Immunohistochemistry, Staining

The hepatic expression of Keap1 in liver tissues of patients with PBC and controls. ( A ) Keap1 protein levels were determined with densitometry analyses, after normalization to GAPDH as a loading control. ( B ) Keap1 mRNA levels were estimated in patients with cirrhotic PBC, patients with early stage PBC, and controls. Results were normalized to 18sRNA. Bars indicate the mean ± SEM. ( C ) Representative immunofluorescence micrographs show liver sections from patients with PBC. (a) Nuclei are stained with DAPI (blue). (b) Immunofluorescence staining of Keap1 (green) shows its abundance in hepatocytes. (c) Arrows indicate the perinuclear and nuclear localizations of Keap1whereas arrowheads indicate cytoplasmic localization of Keap1.
Figure Legend Snippet: The hepatic expression of Keap1 in liver tissues of patients with PBC and controls. ( A ) Keap1 protein levels were determined with densitometry analyses, after normalization to GAPDH as a loading control. ( B ) Keap1 mRNA levels were estimated in patients with cirrhotic PBC, patients with early stage PBC, and controls. Results were normalized to 18sRNA. Bars indicate the mean ± SEM. ( C ) Representative immunofluorescence micrographs show liver sections from patients with PBC. (a) Nuclei are stained with DAPI (blue). (b) Immunofluorescence staining of Keap1 (green) shows its abundance in hepatocytes. (c) Arrows indicate the perinuclear and nuclear localizations of Keap1whereas arrowheads indicate cytoplasmic localization of Keap1.

Techniques Used: Expressing, Immunofluorescence, Staining

2) Product Images from "Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis"

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis

Journal: Scientific Reports

doi: 10.1038/srep44769

Liver expression of Nrf2, Keap1 and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.
Figure Legend Snippet: Liver expression of Nrf2, Keap1 and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.

Techniques Used: Expressing, Immunohistochemistry, Staining

The hepatic expression of Keap1 in liver tissues of patients with PBC and controls. ( A ) Keap1 protein levels were determined with densitometry analyses, after normalization to GAPDH as a loading control. ( B ) Keap1 mRNA levels were estimated in patients with cirrhotic PBC, patients with early stage PBC, and controls. Results were normalized to 18sRNA. Bars indicate the mean ± SEM. ( C ) Representative immunofluorescence micrographs show liver sections from patients with PBC. (a) Nuclei are stained with DAPI (blue). (b) Immunofluorescence staining of Keap1 (green) shows its abundance in hepatocytes. (c) Arrows indicate the perinuclear and nuclear localizations of Keap1whereas arrowheads indicate cytoplasmic localization of Keap1.
Figure Legend Snippet: The hepatic expression of Keap1 in liver tissues of patients with PBC and controls. ( A ) Keap1 protein levels were determined with densitometry analyses, after normalization to GAPDH as a loading control. ( B ) Keap1 mRNA levels were estimated in patients with cirrhotic PBC, patients with early stage PBC, and controls. Results were normalized to 18sRNA. Bars indicate the mean ± SEM. ( C ) Representative immunofluorescence micrographs show liver sections from patients with PBC. (a) Nuclei are stained with DAPI (blue). (b) Immunofluorescence staining of Keap1 (green) shows its abundance in hepatocytes. (c) Arrows indicate the perinuclear and nuclear localizations of Keap1whereas arrowheads indicate cytoplasmic localization of Keap1.

Techniques Used: Expressing, Immunofluorescence, Staining

3) Product Images from "Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway"

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway

Journal: Journal of Cellular Physiology

doi: 10.1002/jcp.29094

Rapamycin suppresses PQ‐induced oxidant stress in pulmonary fibrosis. (a) The expression of ROS level was detected by DCFH‐DA assay. (b) RT‐qPCR was used to measure the expression of NQO1. (c–e) The levels of GSH and CAT, and SOD activity were evaluated by ELISA kit. (f) Western blot was applied to detect the expression of Nrf2, HO‐1, and Keap1 proteins. * p
Figure Legend Snippet: Rapamycin suppresses PQ‐induced oxidant stress in pulmonary fibrosis. (a) The expression of ROS level was detected by DCFH‐DA assay. (b) RT‐qPCR was used to measure the expression of NQO1. (c–e) The levels of GSH and CAT, and SOD activity were evaluated by ELISA kit. (f) Western blot was applied to detect the expression of Nrf2, HO‐1, and Keap1 proteins. * p

Techniques Used: Expressing, DCFH-DA Assay, Quantitative RT-PCR, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot

Related Articles

Immunohistochemistry:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Paragraph title: Immunohistochemistry and immunofluorescence ... For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution).

Avidin-Biotin Assay:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Briefly, sections were treated with Avidin/Biotin Blocking Kit (#SP-2001, Vector Laboratories) and next incubated with 3% H202 diluted in methanol. .. For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution).

Labeling:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz). .. After rinsing with TBST, the membrane was incubated with goat antirabbit IgG labeled with horseradish peroxidase (HRP) (1:5,000; Santa Cruz) or goat antimouse IgG‐HRP (1:5,000; Santa Cruz) at 37°C for 45 min, and explored with ECL reagent (Thermo Fisher Scientific).

Immunofluorescence:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: .. For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution). .. Then, sections were incubated with fluorescein-isothiocyanate (FITC)-conjugated anti-rabbit IgG (#711-095-152, Jackson ImmunoResearch; 1:500 dilution).

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Paragraph title: Immunohistochemistry and immunofluorescence ... Then sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:50 dilution), anti-Nrf2 (Cell Signaling, # 12721 S; 1:20 dilution), anti-CK19 (Santa Cruz, #33119; 1:50 dilution).

Plasmid Preparation:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution). .. Sections were stained with 4’,6diamidino-2-phenylindole (DAPI; H-1200, VECTOR) to visualize cell nuclei.

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Then sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:50 dilution), anti-Nrf2 (Cell Signaling, # 12721 S; 1:20 dilution), anti-CK19 (Santa Cruz, #33119; 1:50 dilution). .. Then, sections were incubated with either biotinylated anti-mouse/anti-rabbit IgG (#BA-1400, Vector Laboratories) or biotinylated anti-goat IgG (#BA-9500, Vector Laboratories).

Concentration Assay:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: 2.8 Western blot Protein was extracted using a whole‐cell lysis kit (CWBio, Beijing, China) from cells and tissues, and the concentration of protein was measured using a BCA protein quantitative kit (Beyotime Biotechnology). .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

Incubation:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Then, sections were incubated with either biotinylated anti-mouse/anti-rabbit IgG (#BA-1400, Vector Laboratories) or biotinylated anti-goat IgG (#BA-9500, Vector Laboratories). .. For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution).

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Briefly, sections were treated with Avidin/Biotin Blocking Kit (#SP-2001, Vector Laboratories) and next incubated with 3% H202 diluted in methanol. .. Then sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:50 dilution), anti-Nrf2 (Cell Signaling, # 12721 S; 1:20 dilution), anti-CK19 (Santa Cruz, #33119; 1:50 dilution).

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz). .. After rinsing with TBST, the membrane was incubated with goat antirabbit IgG labeled with horseradish peroxidase (HRP) (1:5,000; Santa Cruz) or goat antimouse IgG‐HRP (1:5,000; Santa Cruz) at 37°C for 45 min, and explored with ECL reagent (Thermo Fisher Scientific).

Stripping Membranes:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz). .. After removing antibodies by stripping buffer (Beyotime Biotechnology), the membrane was incubated with mouse anti‐β‐actin (1:1,000; Santa Cruz), and goat antimouse IgG‐HRP (1:5,000; Santa Cruz) to detect the internal control, β‐actin.

Blocking Assay:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Briefly, sections were treated with Avidin/Biotin Blocking Kit (#SP-2001, Vector Laboratories) and next incubated with 3% H202 diluted in methanol. .. For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution).

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz). .. After rinsing with TBST, the membrane was incubated with goat antirabbit IgG labeled with horseradish peroxidase (HRP) (1:5,000; Santa Cruz) or goat antimouse IgG‐HRP (1:5,000; Santa Cruz) at 37°C for 45 min, and explored with ECL reagent (Thermo Fisher Scientific).

Polyacrylamide Gel Electrophoresis:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: After being denatured by boiling, the protein sample (40 μg for each lane) was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA). .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

BIA-KA:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: 2.8 Western blot Protein was extracted using a whole‐cell lysis kit (CWBio, Beijing, China) from cells and tissues, and the concentration of protein was measured using a BCA protein quantitative kit (Beyotime Biotechnology). .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

Western Blot:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: Paragraph title: Western blot ... After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

Lysis:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: 2.8 Western blot Protein was extracted using a whole‐cell lysis kit (CWBio, Beijing, China) from cells and tissues, and the concentration of protein was measured using a BCA protein quantitative kit (Beyotime Biotechnology). .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

SDS Page:

Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway
Article Snippet: After being denatured by boiling, the protein sample (40 μg for each lane) was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA). .. After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

Derivative Assay:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: Immunohistochemistry and immunofluorescence Frozen liver sections (6 μm) derived from patients with PBC and controls were fixed in a methanol and acetone mixture (1:1) at −20 °C for 5 min. .. Then sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:50 dilution), anti-Nrf2 (Cell Signaling, # 12721 S; 1:20 dilution), anti-CK19 (Santa Cruz, #33119; 1:50 dilution).

Staining:

Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis
Article Snippet: For immunofluorescence sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:500 dilution). .. Sections were stained with 4’,6diamidino-2-phenylindole (DAPI; H-1200, VECTOR) to visualize cell nuclei.

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    Santa Cruz Biotechnology rabbit anti keap1
    Liver expression of Nrf2, <t>Keap1</t> and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.
    Rabbit Anti Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti keap1/product/Santa Cruz Biotechnology
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit anti keap1 - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Liver expression of Nrf2, Keap1 and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.

    Journal: Scientific Reports

    Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis

    doi: 10.1038/srep44769

    Figure Lengend Snippet: Liver expression of Nrf2, Keap1 and CK19 proteins in patients with cirrhotic PBC and controls. Representative immunohistochemical staining of Nrf2 ( A,B,C,J,K,L ), Keap1 ( D,E,F,M,N,O ) and CK19 ( G,H,I,P,Q,R ) proteins in serial sections of liver tissue from healthy controls (A–I) and cirrhotic PBC (J–R) . In healthy tissue, CK19-positive cells are marked by arrow (large bile duct) or arrowhead (small bile duct). In sections of cirrhotic livers, the corresponding areas are labelled by asterisks. Nrf2 was present only in fibrotic areas (J,K,L), in contrast to Keap1 which was expressed in fibrotic areas as well as in nodules (M,N,O). Original magnification 200x or 400x.

    Article Snippet: Then sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:50 dilution), anti-Nrf2 (Cell Signaling, # 12721 S; 1:20 dilution), anti-CK19 (Santa Cruz, #33119; 1:50 dilution).

    Techniques: Expressing, Immunohistochemistry, Staining

    The hepatic expression of Keap1 in liver tissues of patients with PBC and controls. ( A ) Keap1 protein levels were determined with densitometry analyses, after normalization to GAPDH as a loading control. ( B ) Keap1 mRNA levels were estimated in patients with cirrhotic PBC, patients with early stage PBC, and controls. Results were normalized to 18sRNA. Bars indicate the mean ± SEM. ( C ) Representative immunofluorescence micrographs show liver sections from patients with PBC. (a) Nuclei are stained with DAPI (blue). (b) Immunofluorescence staining of Keap1 (green) shows its abundance in hepatocytes. (c) Arrows indicate the perinuclear and nuclear localizations of Keap1whereas arrowheads indicate cytoplasmic localization of Keap1.

    Journal: Scientific Reports

    Article Title: Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis

    doi: 10.1038/srep44769

    Figure Lengend Snippet: The hepatic expression of Keap1 in liver tissues of patients with PBC and controls. ( A ) Keap1 protein levels were determined with densitometry analyses, after normalization to GAPDH as a loading control. ( B ) Keap1 mRNA levels were estimated in patients with cirrhotic PBC, patients with early stage PBC, and controls. Results were normalized to 18sRNA. Bars indicate the mean ± SEM. ( C ) Representative immunofluorescence micrographs show liver sections from patients with PBC. (a) Nuclei are stained with DAPI (blue). (b) Immunofluorescence staining of Keap1 (green) shows its abundance in hepatocytes. (c) Arrows indicate the perinuclear and nuclear localizations of Keap1whereas arrowheads indicate cytoplasmic localization of Keap1.

    Article Snippet: Then sections were probed with rabbit anti-Keap1 (Santa Cruz, #33569; 1:50 dilution), anti-Nrf2 (Cell Signaling, # 12721 S; 1:20 dilution), anti-CK19 (Santa Cruz, #33119; 1:50 dilution).

    Techniques: Expressing, Immunofluorescence, Staining

    Rapamycin suppresses PQ‐induced oxidant stress in pulmonary fibrosis. (a) The expression of ROS level was detected by DCFH‐DA assay. (b) RT‐qPCR was used to measure the expression of NQO1. (c–e) The levels of GSH and CAT, and SOD activity were evaluated by ELISA kit. (f) Western blot was applied to detect the expression of Nrf2, HO‐1, and Keap1 proteins. * p

    Journal: Journal of Cellular Physiology

    Article Title: Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway, et al. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway

    doi: 10.1002/jcp.29094

    Figure Lengend Snippet: Rapamycin suppresses PQ‐induced oxidant stress in pulmonary fibrosis. (a) The expression of ROS level was detected by DCFH‐DA assay. (b) RT‐qPCR was used to measure the expression of NQO1. (c–e) The levels of GSH and CAT, and SOD activity were evaluated by ELISA kit. (f) Western blot was applied to detect the expression of Nrf2, HO‐1, and Keap1 proteins. * p

    Article Snippet: After blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 hr, the membrane was incubated with the following antibodies at 4°C overnight: rabbit anti‐HO‐1 (1:500; Santa Cruz, CA), rabbit anti‐Keap1 (1:200; Santa Cruz), mouse anti‐α‐SMA (1:400; Boster, Wuhan, Hubei, China), rabbit anti‐Col I (1:400; Boster), mouse anti‐Col III (1:400; Boster), rabbit anti‐MMP‐2 (1:400; Santa Cruz), rabbit anti‐MMP‐9 (1:400; Santa Cruz), rabbit anti‐TIMP (1:400; Santa Cruz), and rabbit anti‐Nrf2 (1:200; (Santa Cruz).

    Techniques: Expressing, DCFH-DA Assay, Quantitative RT-PCR, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot

    Model for Keap1 regulation of Nrf2. Under nonoxidative conditions, Keap1 is evident in focal adhesions (FA). In addition, Keap1 and Nrf2 form a complex in the cytoplasm. This cytoplasmic location is actively maintained by Crm1/exportin, which recognizes an NES present in Keap1. In the cytoplasm, Keap1/Nrf2 is associated with the proteosome, where it is targeted for ubiquitin-mediated degradation. A small amount of the complex shuttled to the nucleus via Nrf2's NLS ensures basal levels of ARE-regulated gene transcription. Inhibition of Crm1/exportin by treatment with LMB results in nuclear translocation of both Keap1 and Nrf2. This represents influx of the cytoplasmic pool of Keap1, as the cytoskeletal pool remains in focal adhesions. Nuclear accumulation of Keap1 and Nrf2 results in the partial activation of the ARE genes. Oxidative stress modifies cysteine residues found within Keap1. This results in the release of Keap1 from the cytoskeleton and from the degradation machinery. All pools of Keap1 and Nrf2 concentrate in the nucleus. A second oxidation sensitive signal, possibly activation of PKC, which phosphorylates Nrf2 (red P), leads to full activation of ARE-regulated genes.

    Journal: Molecular and Cellular Biology

    Article Title: Keap1 Regulates the Oxidation-Sensitive Shuttling of Nrf2 into and out of the Nucleus via a Crm1-Dependent Nuclear Export Mechanism †

    doi: 10.1128/MCB.25.11.4501-4513.2005

    Figure Lengend Snippet: Model for Keap1 regulation of Nrf2. Under nonoxidative conditions, Keap1 is evident in focal adhesions (FA). In addition, Keap1 and Nrf2 form a complex in the cytoplasm. This cytoplasmic location is actively maintained by Crm1/exportin, which recognizes an NES present in Keap1. In the cytoplasm, Keap1/Nrf2 is associated with the proteosome, where it is targeted for ubiquitin-mediated degradation. A small amount of the complex shuttled to the nucleus via Nrf2's NLS ensures basal levels of ARE-regulated gene transcription. Inhibition of Crm1/exportin by treatment with LMB results in nuclear translocation of both Keap1 and Nrf2. This represents influx of the cytoplasmic pool of Keap1, as the cytoskeletal pool remains in focal adhesions. Nuclear accumulation of Keap1 and Nrf2 results in the partial activation of the ARE genes. Oxidative stress modifies cysteine residues found within Keap1. This results in the release of Keap1 from the cytoskeleton and from the degradation machinery. All pools of Keap1 and Nrf2 concentrate in the nucleus. A second oxidation sensitive signal, possibly activation of PKC, which phosphorylates Nrf2 (red P), leads to full activation of ARE-regulated genes.

    Article Snippet: For immunostaining, rabbit anti-Keap1 antibody ( ) was used at 10 μg/ml, rabbit anti-Nrf2 antibody (Santa Cruz, Paso Robles, CA) was used at 1:100, mouse anti-vinculin antibody (Sigma-Aldrich, St. Louis, MO) was used at 1:400, mouse anti-β-catenin antibody (BD Biosciences, San Diego, CA) was used at 1:50, rabbit anti-glutathione S -transferase (GST) alpha (Alpha Diagnostic, Inc., San Antonio, TX) was used at 1:50.

    Techniques: Inhibition, Translocation Assay, Activation Assay

    Keap1 and Nrf2 both redistribute to the nucleus after oxidative stress. (A) Indirect immunofluorescence localization of Keap1 and Nrf2 in NIH 3T3 cells serum starved for 2 h followed by treatment for 24 h with vehicle (dimethyl sulfoxide [DMSO]) or activators of the oxidative stress pathway, DEM, tBHQ, and sulforaphane. Cells were fixed with methanol before incubation with antibodies specific to Keap1 or Nrf2 followed by incubation with an FITC-conjugated secondary antibody. For each panel, an image of the cell nuclei stained with the DNA-specific dye Hoechst (DNA) and a phase-contrast image of the cells (Phase) are also presented. (B) Localization of Keap1 in untreated HepG2 cells and cells treated for 24 h with DEM. Cells were fixed and stained as described for panel A. The corresponding immunofluorescence and phase-contrast images are shown. Bars, 10 μm. (C) Localization of Keap1 in untreated COS7 cells and cells treated with DEM for 2 h. (D) Fractionation of NIH 3T3 cells into nuclear and cytoplasmic fractions. Serum-starved NIH 3T3 cells were left untreated (−) or treated for 2 h with 100 μM DEM (+). Cells were isolated and fractionated into nuclear and cytoplasmic extracts (see Materials and Methods). Equal amounts of protein extracts were loaded in each lane, resolved by SDS-polyacrylamide gel electrophoresis, and probed for Keap1 and Nrf2 by immunoblot analysis. As loading controls, cytoplasmic extracts were probed for GAPDH and nuclear fractions were probed for Lamin A.

    Journal: Molecular and Cellular Biology

    Article Title: Keap1 Regulates the Oxidation-Sensitive Shuttling of Nrf2 into and out of the Nucleus via a Crm1-Dependent Nuclear Export Mechanism †

    doi: 10.1128/MCB.25.11.4501-4513.2005

    Figure Lengend Snippet: Keap1 and Nrf2 both redistribute to the nucleus after oxidative stress. (A) Indirect immunofluorescence localization of Keap1 and Nrf2 in NIH 3T3 cells serum starved for 2 h followed by treatment for 24 h with vehicle (dimethyl sulfoxide [DMSO]) or activators of the oxidative stress pathway, DEM, tBHQ, and sulforaphane. Cells were fixed with methanol before incubation with antibodies specific to Keap1 or Nrf2 followed by incubation with an FITC-conjugated secondary antibody. For each panel, an image of the cell nuclei stained with the DNA-specific dye Hoechst (DNA) and a phase-contrast image of the cells (Phase) are also presented. (B) Localization of Keap1 in untreated HepG2 cells and cells treated for 24 h with DEM. Cells were fixed and stained as described for panel A. The corresponding immunofluorescence and phase-contrast images are shown. Bars, 10 μm. (C) Localization of Keap1 in untreated COS7 cells and cells treated with DEM for 2 h. (D) Fractionation of NIH 3T3 cells into nuclear and cytoplasmic fractions. Serum-starved NIH 3T3 cells were left untreated (−) or treated for 2 h with 100 μM DEM (+). Cells were isolated and fractionated into nuclear and cytoplasmic extracts (see Materials and Methods). Equal amounts of protein extracts were loaded in each lane, resolved by SDS-polyacrylamide gel electrophoresis, and probed for Keap1 and Nrf2 by immunoblot analysis. As loading controls, cytoplasmic extracts were probed for GAPDH and nuclear fractions were probed for Lamin A.

    Article Snippet: For immunostaining, rabbit anti-Keap1 antibody ( ) was used at 10 μg/ml, rabbit anti-Nrf2 antibody (Santa Cruz, Paso Robles, CA) was used at 1:100, mouse anti-vinculin antibody (Sigma-Aldrich, St. Louis, MO) was used at 1:400, mouse anti-β-catenin antibody (BD Biosciences, San Diego, CA) was used at 1:50, rabbit anti-glutathione S -transferase (GST) alpha (Alpha Diagnostic, Inc., San Antonio, TX) was used at 1:50.

    Techniques: Immunofluorescence, Incubation, Staining, Fractionation, Isolation, Polyacrylamide Gel Electrophoresis

    ). aa, amino acid. (B) GFP fusion constructs used to analyze the NES of Keap1. The amino acid boundaries of each construct above each diagram and the positions of the BTB, IVR, and Kelch repeat domains are shown. The position of the NES is shown with an asterisk. (C) Localization of Keap1-GFP fusion constructs in untreated NIH 3T3 cells and cells treated with 3 nM LMB for 3 h. Images were taken of live cells. Fluorescence images are arranged with the GFP fluorescence in the left panel (GFP) and the Hoechst stain of DNA in the right panel (DNA). Keap1-GFP and NES-Kelch-GFP are predominantly cytoplasmic but redistribute to the nucleus after LMB treatment. Other constructs are unaffected by LMB treatment and exhibit both nuclear and cytoplasmic locations. Bar, 10 μm. (D) A histogram quantifying the localization of the GFP constructs to the nucleus. Two hundred cells were counted for each construct. N

    Journal: Molecular and Cellular Biology

    Article Title: Keap1 Regulates the Oxidation-Sensitive Shuttling of Nrf2 into and out of the Nucleus via a Crm1-Dependent Nuclear Export Mechanism †

    doi: 10.1128/MCB.25.11.4501-4513.2005

    Figure Lengend Snippet: ). aa, amino acid. (B) GFP fusion constructs used to analyze the NES of Keap1. The amino acid boundaries of each construct above each diagram and the positions of the BTB, IVR, and Kelch repeat domains are shown. The position of the NES is shown with an asterisk. (C) Localization of Keap1-GFP fusion constructs in untreated NIH 3T3 cells and cells treated with 3 nM LMB for 3 h. Images were taken of live cells. Fluorescence images are arranged with the GFP fluorescence in the left panel (GFP) and the Hoechst stain of DNA in the right panel (DNA). Keap1-GFP and NES-Kelch-GFP are predominantly cytoplasmic but redistribute to the nucleus after LMB treatment. Other constructs are unaffected by LMB treatment and exhibit both nuclear and cytoplasmic locations. Bar, 10 μm. (D) A histogram quantifying the localization of the GFP constructs to the nucleus. Two hundred cells were counted for each construct. N

    Article Snippet: For immunostaining, rabbit anti-Keap1 antibody ( ) was used at 10 μg/ml, rabbit anti-Nrf2 antibody (Santa Cruz, Paso Robles, CA) was used at 1:100, mouse anti-vinculin antibody (Sigma-Aldrich, St. Louis, MO) was used at 1:400, mouse anti-β-catenin antibody (BD Biosciences, San Diego, CA) was used at 1:50, rabbit anti-glutathione S -transferase (GST) alpha (Alpha Diagnostic, Inc., San Antonio, TX) was used at 1:50.

    Techniques: Construct, Fluorescence, Staining