rabbit anti human sma  (Abcam)

 
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    Name:
    Rabbit Anti Human IgM mu chain Alkaline Phosphatase
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    Catalog Number:
    AB97207
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    Structured Review

    Abcam rabbit anti human sma

    https://www.bioz.com/result/rabbit anti human sma/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human sma - by Bioz Stars, 2021-06
    99/100 stars

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    Labeling:

    Article Title: Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease
    Article Snippet: .. Antigen retrieval was performed in 10 mM citrate buffer, pH 6.0, and microwaved for 4–5 min. Non-specific labeling was blocked by 1% BSA in PBS and then sections were incubated with primary antibodies rabbit anti-human SOD2 (1:100; Millipore) and rabbit anti-human CAII (1:5,000, Abcam) followed by 12-nm gold conjugated goat anti-rabbit IgG (1:20; Jackson ImmunoResearch Laboratories). ..

    Incubation:

    Article Title: Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease
    Article Snippet: .. Antigen retrieval was performed in 10 mM citrate buffer, pH 6.0, and microwaved for 4–5 min. Non-specific labeling was blocked by 1% BSA in PBS and then sections were incubated with primary antibodies rabbit anti-human SOD2 (1:100; Millipore) and rabbit anti-human CAII (1:5,000, Abcam) followed by 12-nm gold conjugated goat anti-rabbit IgG (1:20; Jackson ImmunoResearch Laboratories). ..

    Article Title: Generation of Gellan Gum-Based Adipose-Like Microtissues
    Article Snippet: Immunocytochemistry Cell-laden hydrogel particles were fixed in 10% v /v of formalin (Bio-Optic, Milano, Italy) for 1 h, incubated with 1% Triton X-100 (Sigma-Aldrich, Sintra, Portugal) for 20 min at 4 °C, and with 2.5% w /v of horse serum (HS, Vector Laboratories, Burlingame, CA, USA) for 1 h, respectively for cell permeabilization and blocking of nonspecific antibody binding. .. Samples were then incubated with rabbit anti-human primary antibodies FABP4 (1:100), PPAR gamma (1:25) (Abcam, Cambridge, UK) diluted in 1% BSA, 0.2% Triton in PBS for 24 h. After washing with PBS, samples were incubated overnight at 4 °C with the secondary antibody Alexa Fluor 488 donkey anti-rabbit (Life Technologies, Carlsband, CA, USA) at a concentration of 1:500 in 1% HS in PBS. ..

    Article Title: Selective and marked decrease of complement receptor C5aR2 in human thoracic aortic aneurysms: a dysregulation with potential inflammatory effects
    Article Snippet: Immunofluorescence Formalin fixed, paraffin-embedded 5 µm thick sections were deparaffinised and rehydrated, blocked with BLOXALL (SP-6000, Vector labs, Burlingame, California, USA) and treated with citrate buffer (pH 6) to retrieve epitopes. .. Sections were rinsed in tris buffered saline with 0.1% Tween 20 (TBST) two times, blocked with 2.5% normal goat serum plus 2.5% normal horse serum (S-1012 and S-2012, Vector labs), and incubated with the following primary antibodies; rat anti-mouse/human Mac-2 (1:600, Cedarlane, Burlington, Canada), rabbit anti-human αSMA (1:200, Abcam) and rabbit anti-human CD3 (1:50, Abcam) at room temperature for 1 hour. .. Rat-on-mouse AP-Polymer (RT518H, Biocare Medical, Pacheco, California, USA) was used for sections stained with anti-Mac-2, according to manufacturer’s recommendations.

    Article Title: Genetic variants in de novo lipogenic pathway genes predict the prognosis of surgically-treated hepatocellular carcinoma
    Article Snippet: After three washes in phosphate-buffered saline (PBS), every five minutes, the slides were boiled in antigen retrieval buffer containing 0.01 mol/L citrate antigen retrieval (pH = 6.0) in the pressure cooker and then rinsed in peroxidase quenching solution (Invitrogen) to block endogenous peroxidase. .. The sections were then incubated with a rabbit anti-human ACACA antibody (1:200, Abcam) or rabbit anti-human FASN antibody (1:200, Abcam) at 4°C overnight and then with an Broad Spectrum Second Antibody (Invitrogen) at 37°C for 20 min. After three washes, the visualization signal was developed with Invitrogen Histostain Plus kit. ..

    Article Title: HIF-1α induces the epithelial-mesenchymal transition in gastric cancer stem cells through the Snail pathway
    Article Snippet: Fifty micrograms of each protein sample was loaded onto 8% SDS–PAGE gels and subjected to electrophoresis under denaturing conditions followed by transfer to PVDF membranes (Millipore). .. The membranes were blocked with 5% BSA in TBST for 2 h at room temperature and then incubated with a rabbit anti-human E-cadherin (1:400, Abcam, USA), rabbit anti-human Vimentin (1:400, Abcam, USA), mouse anti-human N-cadherin (1:400, Abcam, USA), rabbit anti-human Nanog (1:300, Abcam, USA), rabbit anti-human Oct4 (1:300, Abcam, USA), rabbit anti-human HIF-1α (1:500, Abcam, USA), or rabbit anti-human Snail (1:500, Abcam, USA) antibody overnight at 4°C. .. Rabbit anti-human GAPDH (1:1000, CWBIO, China) was used as an endogenous control.

    Article Title: The promotion of angiogenesis induced by three-dimensional porous beta-tricalcium phosphate scaffold with different interconnection sizes via activation of PI3K/Akt pathways
    Article Snippet: .. The fixed cell/scaffold was placed in 3% bovine serum albumin (BSA) blocking buffer for 1 h. After incubating with rabbit anti-human CD31 primary antibody (1:50, Abcam) overnight at 4°C, the cell/scaffold was incubated in an anti-rabbit secondary antibody Alexa Fluor ®594 (1:1000; 2 μg ml−1 , Invitrogen) for 1 h at room temperature. ..

    Concentration Assay:

    Article Title: Generation of Gellan Gum-Based Adipose-Like Microtissues
    Article Snippet: Immunocytochemistry Cell-laden hydrogel particles were fixed in 10% v /v of formalin (Bio-Optic, Milano, Italy) for 1 h, incubated with 1% Triton X-100 (Sigma-Aldrich, Sintra, Portugal) for 20 min at 4 °C, and with 2.5% w /v of horse serum (HS, Vector Laboratories, Burlingame, CA, USA) for 1 h, respectively for cell permeabilization and blocking of nonspecific antibody binding. .. Samples were then incubated with rabbit anti-human primary antibodies FABP4 (1:100), PPAR gamma (1:25) (Abcam, Cambridge, UK) diluted in 1% BSA, 0.2% Triton in PBS for 24 h. After washing with PBS, samples were incubated overnight at 4 °C with the secondary antibody Alexa Fluor 488 donkey anti-rabbit (Life Technologies, Carlsband, CA, USA) at a concentration of 1:500 in 1% HS in PBS. ..

    Blocking Assay:

    Article Title: The promotion of angiogenesis induced by three-dimensional porous beta-tricalcium phosphate scaffold with different interconnection sizes via activation of PI3K/Akt pathways
    Article Snippet: .. The fixed cell/scaffold was placed in 3% bovine serum albumin (BSA) blocking buffer for 1 h. After incubating with rabbit anti-human CD31 primary antibody (1:50, Abcam) overnight at 4°C, the cell/scaffold was incubated in an anti-rabbit secondary antibody Alexa Fluor ®594 (1:1000; 2 μg ml−1 , Invitrogen) for 1 h at room temperature. ..

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  • 94
    Abcam rabbit anti human α sma
    IL-17A induces proliferation, collagen synthesis and secretion of SSc patient-derived DVSMCs via ERK1/2 signing pathway. (A) DVSMCs were pre-treated with PD 98059 (10 μM/ml) for 2 h before incubation with IL-17A at 100 ng/ml for 24 h, 48 h, 72 h, and cell proliferation was tested using cell counting kit-8. (B) The cells were pre-treated with PD98059 for 2 h before incubation with the serum of SSc patients or healthy individuals for 24 h, 48 h, 72 h, and cell proliferation was tested using CCK8. (C) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A at 100 ng/ml for 24 h. The activity of collagen1α1 or collagen3α1 proximal promoter was detected using a dual luciferase reporter gene assay. (D) After being pre-treated with PD98059 for 2h before incubation with the serum of SSc patients or healthy individuals for 24 h, the activity of collagen1α1 or collagen3α1 proximal promoter was also detected. Data were represented as mean ratios of Firefly to Renilla luciferase activity. (E, F) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A or SSc serum for 24 h, the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (G, H) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A or SSc serum for 24 h, the concentration of collagen 1, collagen 3 was detected using ELISA. (I, J) The cells were pre-treated with PD98059 for 2 h before incubation with IL-17A, serum from SSc patients and healthy individuals for 24 h, and the protein expressions of collagen 1, collagen 3, IL-17RA and <t>α-SMA</t> were measured using Western blot. GAPDH was used as a loading control. The experiment was repeated three times, and the data are presented as means ± standard deviation.
    Rabbit Anti Human α Sma, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human α sma/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human α sma - by Bioz Stars, 2021-06
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    93
    Abcam anti human alpha smooth muscle actin α sma rabbit polyclonal antibody
    EB formation and expression of three germ layer markers. (A) EBs of ∼200 μm in size were formed by using a floating culture plate with EB medium. (B) Paraffin-embedded sections of the EBs were prepared and stained with hematoxylin and eosin and the morphology was examined using light microscopy. The cells were fully contained, even in the core of the EBs, and the cell morphology within the core of EBs was maintained. Immunostaining of EBs for (C) tubulin-β3, an ectoderm marker, (D) <t>α-SMA,</t> a mesoderm marker, and (E) anti-human α-fetoprotein, an endoderm marker, revealed positive cells at different sites (scale bar = 50 μm). (F) The expression level of self-renewal genes in two commercially available undifferentiated iPS cell lines, and human monocyte-derived iPS cells, and the expression level of ectodermal genes, mesodermal genes, and endodermal genes after differentiation of the cells into EBs were similar. EB, embryoid body; α-SMA, <t>alpha-smooth</t> muscle actin.
    Anti Human Alpha Smooth Muscle Actin α Sma Rabbit Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human alpha smooth muscle actin α sma rabbit polyclonal antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human alpha smooth muscle actin α sma rabbit polyclonal antibody - by Bioz Stars, 2021-06
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    95
    Abcam monoclonal rabbit anti human α smooth muscle actin
    Expression of E-cadherin and α-SMA in RPE cells. Immunofluorescence staining for (A) E-cadherin (red) in RPE cells in the (a) normal control group, (b) TGF-β2-treated group, and (c) VAS2870 group exposed to TGF-β2. (B) Expression of α-SMA (green) in RPE cells in the (a) normal control group, (b) TGF-β2-treated group and (c) VAS2870 group exposed to TGF-β2. Representative images are shown (magnification, x200; Scale bar=100 µ m). TGF-β2, transforming growth factor-β2; α-SMA, <t>α-smooth</t> muscle actin.
    Monoclonal Rabbit Anti Human α Smooth Muscle Actin, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rabbit anti human α smooth muscle actin/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal rabbit anti human α smooth muscle actin - by Bioz Stars, 2021-06
    95/100 stars
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    IL-17A induces proliferation, collagen synthesis and secretion of SSc patient-derived DVSMCs via ERK1/2 signing pathway. (A) DVSMCs were pre-treated with PD 98059 (10 μM/ml) for 2 h before incubation with IL-17A at 100 ng/ml for 24 h, 48 h, 72 h, and cell proliferation was tested using cell counting kit-8. (B) The cells were pre-treated with PD98059 for 2 h before incubation with the serum of SSc patients or healthy individuals for 24 h, 48 h, 72 h, and cell proliferation was tested using CCK8. (C) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A at 100 ng/ml for 24 h. The activity of collagen1α1 or collagen3α1 proximal promoter was detected using a dual luciferase reporter gene assay. (D) After being pre-treated with PD98059 for 2h before incubation with the serum of SSc patients or healthy individuals for 24 h, the activity of collagen1α1 or collagen3α1 proximal promoter was also detected. Data were represented as mean ratios of Firefly to Renilla luciferase activity. (E, F) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A or SSc serum for 24 h, the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (G, H) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A or SSc serum for 24 h, the concentration of collagen 1, collagen 3 was detected using ELISA. (I, J) The cells were pre-treated with PD98059 for 2 h before incubation with IL-17A, serum from SSc patients and healthy individuals for 24 h, and the protein expressions of collagen 1, collagen 3, IL-17RA and α-SMA were measured using Western blot. GAPDH was used as a loading control. The experiment was repeated three times, and the data are presented as means ± standard deviation.

    Journal: Arthritis Research & Therapy

    Article Title: Interleukin-17A promotes functional activation of systemic sclerosis patient-derived dermal vascular smooth muscle cells by extracellular-regulated protein kinases signalling pathway

    doi: 10.1186/s13075-014-0512-2

    Figure Lengend Snippet: IL-17A induces proliferation, collagen synthesis and secretion of SSc patient-derived DVSMCs via ERK1/2 signing pathway. (A) DVSMCs were pre-treated with PD 98059 (10 μM/ml) for 2 h before incubation with IL-17A at 100 ng/ml for 24 h, 48 h, 72 h, and cell proliferation was tested using cell counting kit-8. (B) The cells were pre-treated with PD98059 for 2 h before incubation with the serum of SSc patients or healthy individuals for 24 h, 48 h, 72 h, and cell proliferation was tested using CCK8. (C) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A at 100 ng/ml for 24 h. The activity of collagen1α1 or collagen3α1 proximal promoter was detected using a dual luciferase reporter gene assay. (D) After being pre-treated with PD98059 for 2h before incubation with the serum of SSc patients or healthy individuals for 24 h, the activity of collagen1α1 or collagen3α1 proximal promoter was also detected. Data were represented as mean ratios of Firefly to Renilla luciferase activity. (E, F) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A or SSc serum for 24 h, the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (G, H) The cells were pre-treated with PD 98059 for 2 h before incubation with IL-17A or SSc serum for 24 h, the concentration of collagen 1, collagen 3 was detected using ELISA. (I, J) The cells were pre-treated with PD98059 for 2 h before incubation with IL-17A, serum from SSc patients and healthy individuals for 24 h, and the protein expressions of collagen 1, collagen 3, IL-17RA and α-SMA were measured using Western blot. GAPDH was used as a loading control. The experiment was repeated three times, and the data are presented as means ± standard deviation.

    Article Snippet: After being blocked with 5% milk for 2 h at room temperature, the membranes were incubated using primary antibodies, including rabbit anti-human collagen 1 (1:1000, Abcam), rabbit anti-human collagen 3 (1:3000, Abcam), rabbit anti-human α-SMA (1:600, Abcam), goat anti-human IL-17RA (1:1000, Abcam), rabbit anti-human p38 (1:1000, Cell Signaling Technology, Boston, MA, USA), rabbit anti-human phospho-p38 MAPK (1:1000, Cell Signaling Technology), rabbit anti-human JNK (1:1000, Cell Signaling Technology), rabbit anti-human phospho-JNK (1:1000, Cell Signaling Technology), rabbit anti-human ERK1/2 (1:1000, Cell Signaling Technology), rabbit anti-human phospho-ERK1/2 (1:1000, Cell Signaling Technology), and rabbit anti-human GAPDH (1:1000, Cell Signaling Technology) overnight at 4°C.

    Techniques: Derivative Assay, Incubation, Cell Counting, Activity Assay, Luciferase, Reporter Gene Assay, Expressing, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Standard Deviation

    IL-17A derived from SSc patient serum promotes the proliferation, collagen synthesis and secretion of SSc patient-derived DVSMCs. (A) DVSMCs were treated with different doses of IL-17A for 24 h, 48 h, 72 h, and cell proliferation was tested using cell counting kit-8. (B) The cells were treated with the serum of SSc patients for 24 h, 48 h, 72 h, and cell proliferation was tested using CCK8. (C) The activity of collagen1α1 or collagen3α1 proximal promoter was test using a dual luciferase reporter gene assay after being treated with different doses of IL-17A for 24 h. (D) The activity of collagen1α1 or collagen3α1 proximal promoter was also detected after being treated with the serum of SSc patients and healthy subject for 24 h. Data were represented as mean ratios of Firefly to Renilla luciferase activity. (E) The cells were cultured in the indicated doses of IL-17A for 24 h, the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (F) The cells were stimulated with the serum of SSc patients and healthy subjects for 24 h, and the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (G) The cells were treated with different doses of IL-17A for 24 h, the concentration of collagen 1, collagen 3 was detected using ELISA. (H) The cells were treated with the serum of SSc patients and healthy subjects for 24 h, and the concentration of collagen 1, collagen 3 was also detected by ELISA. (I, J) The cells were treated with different doses of IL-17A, serum of SSc patients or healthy individuals for 24 h, and the expressions of collagen 1, collagen 3, IL-17RA and α-SMA were measured using Western blot. GAPDH was used as a loading control. The experiment was repeated three times, and the data are presented as means ± standard deviation.

    Journal: Arthritis Research & Therapy

    Article Title: Interleukin-17A promotes functional activation of systemic sclerosis patient-derived dermal vascular smooth muscle cells by extracellular-regulated protein kinases signalling pathway

    doi: 10.1186/s13075-014-0512-2

    Figure Lengend Snippet: IL-17A derived from SSc patient serum promotes the proliferation, collagen synthesis and secretion of SSc patient-derived DVSMCs. (A) DVSMCs were treated with different doses of IL-17A for 24 h, 48 h, 72 h, and cell proliferation was tested using cell counting kit-8. (B) The cells were treated with the serum of SSc patients for 24 h, 48 h, 72 h, and cell proliferation was tested using CCK8. (C) The activity of collagen1α1 or collagen3α1 proximal promoter was test using a dual luciferase reporter gene assay after being treated with different doses of IL-17A for 24 h. (D) The activity of collagen1α1 or collagen3α1 proximal promoter was also detected after being treated with the serum of SSc patients and healthy subject for 24 h. Data were represented as mean ratios of Firefly to Renilla luciferase activity. (E) The cells were cultured in the indicated doses of IL-17A for 24 h, the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (F) The cells were stimulated with the serum of SSc patients and healthy subjects for 24 h, and the gene expression of collagen 1 and collagen 3 was measured using real-time RT-PCR analysis. (G) The cells were treated with different doses of IL-17A for 24 h, the concentration of collagen 1, collagen 3 was detected using ELISA. (H) The cells were treated with the serum of SSc patients and healthy subjects for 24 h, and the concentration of collagen 1, collagen 3 was also detected by ELISA. (I, J) The cells were treated with different doses of IL-17A, serum of SSc patients or healthy individuals for 24 h, and the expressions of collagen 1, collagen 3, IL-17RA and α-SMA were measured using Western blot. GAPDH was used as a loading control. The experiment was repeated three times, and the data are presented as means ± standard deviation.

    Article Snippet: After being blocked with 5% milk for 2 h at room temperature, the membranes were incubated using primary antibodies, including rabbit anti-human collagen 1 (1:1000, Abcam), rabbit anti-human collagen 3 (1:3000, Abcam), rabbit anti-human α-SMA (1:600, Abcam), goat anti-human IL-17RA (1:1000, Abcam), rabbit anti-human p38 (1:1000, Cell Signaling Technology, Boston, MA, USA), rabbit anti-human phospho-p38 MAPK (1:1000, Cell Signaling Technology), rabbit anti-human JNK (1:1000, Cell Signaling Technology), rabbit anti-human phospho-JNK (1:1000, Cell Signaling Technology), rabbit anti-human ERK1/2 (1:1000, Cell Signaling Technology), rabbit anti-human phospho-ERK1/2 (1:1000, Cell Signaling Technology), and rabbit anti-human GAPDH (1:1000, Cell Signaling Technology) overnight at 4°C.

    Techniques: Derivative Assay, Cell Counting, Activity Assay, Luciferase, Reporter Gene Assay, Cell Culture, Expressing, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Standard Deviation

    Characterization of human PSCs (hPSCs). Lipid droplets were visualized with oil red O staining (original magnifications, ×400). Immunofluorescence was performed using antibodies against α-SMA, glial fibrillary acidic protein (GFAP), and vimentin (original magnification, ×200), and the cell nucleus was stained with DAPI (blue). Negative control, without primary antibodies.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: BMP2 inhibits TGF-?-induced pancreatic stellate cell activation and extracellular matrix formation

    doi: 10.1152/ajpgi.00306.2012

    Figure Lengend Snippet: Characterization of human PSCs (hPSCs). Lipid droplets were visualized with oil red O staining (original magnifications, ×400). Immunofluorescence was performed using antibodies against α-SMA, glial fibrillary acidic protein (GFAP), and vimentin (original magnification, ×200), and the cell nucleus was stained with DAPI (blue). Negative control, without primary antibodies.

    Article Snippet: The sections were then incubated with a rabbit anti-human α-SMA monoclonal antibody (1:200 dilution in 3% BSA; Abcam, Cambridge, MA) overnight at 4°C followed by incubation with a biotinylated anti-rabbit antibody for 1 h at room temperature, and then with ABC regents for 30 min at room temperature.

    Techniques: Staining, Immunofluorescence, Negative Control

    BMP2 inhibits transforming growth factor (TGF)-β-induced mouse PSC (mPSC) activation and FN formation. mPSCs were pretreated with 250 ng/ml of BMP2 for 30 min followed by 1 ng/ml TGF-β for 48 h. A : immunofluorescence was performed using antibodies against α-SMA (red) and FN (green), and the cell nucleus was stained with DAPI (blue). B : quantification of fluorescence intensity from 5–10 different fields/sample. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: BMP2 inhibits TGF-?-induced pancreatic stellate cell activation and extracellular matrix formation

    doi: 10.1152/ajpgi.00306.2012

    Figure Lengend Snippet: BMP2 inhibits transforming growth factor (TGF)-β-induced mouse PSC (mPSC) activation and FN formation. mPSCs were pretreated with 250 ng/ml of BMP2 for 30 min followed by 1 ng/ml TGF-β for 48 h. A : immunofluorescence was performed using antibodies against α-SMA (red) and FN (green), and the cell nucleus was stained with DAPI (blue). B : quantification of fluorescence intensity from 5–10 different fields/sample. * P

    Article Snippet: The sections were then incubated with a rabbit anti-human α-SMA monoclonal antibody (1:200 dilution in 3% BSA; Abcam, Cambridge, MA) overnight at 4°C followed by incubation with a biotinylated anti-rabbit antibody for 1 h at room temperature, and then with ABC regents for 30 min at room temperature.

    Techniques: Activation Assay, Immunofluorescence, Staining, Fluorescence

    Smad1 signaling pathway mediates the inhibitory effect of BMP2 on TGF-β-induced hPSC activation and FN production. A : hPSCs were treated with BMP2 for 30 min. Whole cell lysates were subject to Western blotting using pSmad1 and total Smad1 antibody on the same blot. B : Smad1 small-interfering RNA (siRNA) transfection efficiency. hPSCs were transfected with human Smad1 siRNA for 48 h, starved for 4 h, and then pretreated with BMP2 (250 ng/ml) for 30 min followed by TGF-β (1 ng/ml) for 30 min. Whole cell lysates were subject to Western blotting using Smad1 and pSmad1 antibodies on the same blot. C and D : hPSCs were transfected with human Smad1 siRNA for 48 h, starved for 4 h, and then pretreated with BMP2 (250 ng/ml) for 30 min followed by TGF-β (1 ng/ml) for 48 h. Immunofluorescence was performed using α-SMA (red) antibody and the respective quantification ( C ) or using FN (green) antibody and the respective quantification ( D ). Cell nucleus was stained with DAPI (blue). NS siRNA, nonspecific siRNA. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: BMP2 inhibits TGF-?-induced pancreatic stellate cell activation and extracellular matrix formation

    doi: 10.1152/ajpgi.00306.2012

    Figure Lengend Snippet: Smad1 signaling pathway mediates the inhibitory effect of BMP2 on TGF-β-induced hPSC activation and FN production. A : hPSCs were treated with BMP2 for 30 min. Whole cell lysates were subject to Western blotting using pSmad1 and total Smad1 antibody on the same blot. B : Smad1 small-interfering RNA (siRNA) transfection efficiency. hPSCs were transfected with human Smad1 siRNA for 48 h, starved for 4 h, and then pretreated with BMP2 (250 ng/ml) for 30 min followed by TGF-β (1 ng/ml) for 30 min. Whole cell lysates were subject to Western blotting using Smad1 and pSmad1 antibodies on the same blot. C and D : hPSCs were transfected with human Smad1 siRNA for 48 h, starved for 4 h, and then pretreated with BMP2 (250 ng/ml) for 30 min followed by TGF-β (1 ng/ml) for 48 h. Immunofluorescence was performed using α-SMA (red) antibody and the respective quantification ( C ) or using FN (green) antibody and the respective quantification ( D ). Cell nucleus was stained with DAPI (blue). NS siRNA, nonspecific siRNA. * P

    Article Snippet: The sections were then incubated with a rabbit anti-human α-SMA monoclonal antibody (1:200 dilution in 3% BSA; Abcam, Cambridge, MA) overnight at 4°C followed by incubation with a biotinylated anti-rabbit antibody for 1 h at room temperature, and then with ABC regents for 30 min at room temperature.

    Techniques: Activation Assay, Western Blot, Small Interfering RNA, Transfection, Immunofluorescence, Staining

    Time-dependent increase of α-smooth muscle actin (α-SMA) in CP mice. A : immunohistochemical staining of pancreas paraffin section from CON and CP mice using α-SMA antibody. α-SMA-positive cells [activated pancreatic stellate cells (PSCs)] are present in the periacinar fibrotic areas with the stellate morphology and vascular walls in CP specimens, but are present mostly in the vascular walls in CON specimens. B : α-SMA-positive cells in the periacinar areas were quantified based on 6–10 different fields/slide. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: BMP2 inhibits TGF-?-induced pancreatic stellate cell activation and extracellular matrix formation

    doi: 10.1152/ajpgi.00306.2012

    Figure Lengend Snippet: Time-dependent increase of α-smooth muscle actin (α-SMA) in CP mice. A : immunohistochemical staining of pancreas paraffin section from CON and CP mice using α-SMA antibody. α-SMA-positive cells [activated pancreatic stellate cells (PSCs)] are present in the periacinar fibrotic areas with the stellate morphology and vascular walls in CP specimens, but are present mostly in the vascular walls in CON specimens. B : α-SMA-positive cells in the periacinar areas were quantified based on 6–10 different fields/slide. * P

    Article Snippet: The sections were then incubated with a rabbit anti-human α-SMA monoclonal antibody (1:200 dilution in 3% BSA; Abcam, Cambridge, MA) overnight at 4°C followed by incubation with a biotinylated anti-rabbit antibody for 1 h at room temperature, and then with ABC regents for 30 min at room temperature.

    Techniques: Mouse Assay, Immunohistochemistry, Staining, Paraffin Section

    BMP2 inhibits TGF-β-induced α-SMA and FN expression in hPSCs. hPSCs were pretreated with BMP2 (250 ng/ml) for 30 min followed by TGF-β (1 ng/ml) for 48 h. A : immunofluorescence was performed using antibodies against α-SMA (red) and FN (green), and the cell nucleus was stained with DAPI (blue). B : quantification of fluorescent intensity from 5–10 different fields/sample. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: BMP2 inhibits TGF-?-induced pancreatic stellate cell activation and extracellular matrix formation

    doi: 10.1152/ajpgi.00306.2012

    Figure Lengend Snippet: BMP2 inhibits TGF-β-induced α-SMA and FN expression in hPSCs. hPSCs were pretreated with BMP2 (250 ng/ml) for 30 min followed by TGF-β (1 ng/ml) for 48 h. A : immunofluorescence was performed using antibodies against α-SMA (red) and FN (green), and the cell nucleus was stained with DAPI (blue). B : quantification of fluorescent intensity from 5–10 different fields/sample. * P

    Article Snippet: The sections were then incubated with a rabbit anti-human α-SMA monoclonal antibody (1:200 dilution in 3% BSA; Abcam, Cambridge, MA) overnight at 4°C followed by incubation with a biotinylated anti-rabbit antibody for 1 h at room temperature, and then with ABC regents for 30 min at room temperature.

    Techniques: Expressing, Immunofluorescence, Staining

    EB formation and expression of three germ layer markers. (A) EBs of ∼200 μm in size were formed by using a floating culture plate with EB medium. (B) Paraffin-embedded sections of the EBs were prepared and stained with hematoxylin and eosin and the morphology was examined using light microscopy. The cells were fully contained, even in the core of the EBs, and the cell morphology within the core of EBs was maintained. Immunostaining of EBs for (C) tubulin-β3, an ectoderm marker, (D) α-SMA, a mesoderm marker, and (E) anti-human α-fetoprotein, an endoderm marker, revealed positive cells at different sites (scale bar = 50 μm). (F) The expression level of self-renewal genes in two commercially available undifferentiated iPS cell lines, and human monocyte-derived iPS cells, and the expression level of ectodermal genes, mesodermal genes, and endodermal genes after differentiation of the cells into EBs were similar. EB, embryoid body; α-SMA, alpha-smooth muscle actin.

    Journal: Cellular Reprogramming

    Article Title: Preparation of Induced Pluripotent Stem Cells Using Human Peripheral Blood Monocytes

    doi: 10.1089/cell.2018.0024

    Figure Lengend Snippet: EB formation and expression of three germ layer markers. (A) EBs of ∼200 μm in size were formed by using a floating culture plate with EB medium. (B) Paraffin-embedded sections of the EBs were prepared and stained with hematoxylin and eosin and the morphology was examined using light microscopy. The cells were fully contained, even in the core of the EBs, and the cell morphology within the core of EBs was maintained. Immunostaining of EBs for (C) tubulin-β3, an ectoderm marker, (D) α-SMA, a mesoderm marker, and (E) anti-human α-fetoprotein, an endoderm marker, revealed positive cells at different sites (scale bar = 50 μm). (F) The expression level of self-renewal genes in two commercially available undifferentiated iPS cell lines, and human monocyte-derived iPS cells, and the expression level of ectodermal genes, mesodermal genes, and endodermal genes after differentiation of the cells into EBs were similar. EB, embryoid body; α-SMA, alpha-smooth muscle actin.

    Article Snippet: As primary antibodies, anti-human tubulin-β3 mouse monoclonal antibody (1:100; Biolegend, Inc., San Diego, CA) as an ectoderm marker protein, anti-human alpha-smooth muscle actin (α-SMA) rabbit polyclonal antibody (1:100; Abcam) as a mesoderm marker protein, and anti-human α-fetoprotein (AFP) rabbit polyclonal antibody (Proteintech Group, Inc., Rosemont, IL) as an endoderm marker protein were added and incubated at 37°C for 1 hour.

    Techniques: Expressing, Staining, Light Microscopy, Immunostaining, Marker, Derivative Assay

    Expression of E-cadherin and α-SMA in RPE cells. Immunofluorescence staining for (A) E-cadherin (red) in RPE cells in the (a) normal control group, (b) TGF-β2-treated group, and (c) VAS2870 group exposed to TGF-β2. (B) Expression of α-SMA (green) in RPE cells in the (a) normal control group, (b) TGF-β2-treated group and (c) VAS2870 group exposed to TGF-β2. Representative images are shown (magnification, x200; Scale bar=100 µ m). TGF-β2, transforming growth factor-β2; α-SMA, α-smooth muscle actin.

    Journal: International Journal of Molecular Medicine

    Article Title: Novel NADPH oxidase inhibitor VAS2870 suppresses TGF-β-dependent epithelial-to-mesenchymal transition in retinal pigment epithelial cells

    doi: 10.3892/ijmm.2018.3612

    Figure Lengend Snippet: Expression of E-cadherin and α-SMA in RPE cells. Immunofluorescence staining for (A) E-cadherin (red) in RPE cells in the (a) normal control group, (b) TGF-β2-treated group, and (c) VAS2870 group exposed to TGF-β2. (B) Expression of α-SMA (green) in RPE cells in the (a) normal control group, (b) TGF-β2-treated group and (c) VAS2870 group exposed to TGF-β2. Representative images are shown (magnification, x200; Scale bar=100 µ m). TGF-β2, transforming growth factor-β2; α-SMA, α-smooth muscle actin.

    Article Snippet: The cells were then permeabilized with 0.3% Triton X-100 for 20 min at room temperature, and slides were blocked in goat serum (Boster Biological Technology, Pleasanton, CA, USA) for 1 h, followed by overnight incubation at 4°C with the following primary antibodies: Monoclonal rabbit anti-human α-smooth muscle actin (SMA; 1:200; cat. no. ab124964; Abcam, Cambridge, MA, USA) and monoclonal rabbit anti-human E-cadherin (1:200; cat. no. ab53226; Abcam).

    Techniques: Expressing, Immunofluorescence, Staining