rabbit anti goat igg hrp conjugated antibody  (Thermo Fisher)


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    Name:
    Rabbit anti Goat IgG H L Secondary Antibody
    Description:
    Rabbit anti Goat IgG H L Secondary Antibody for Western Blot IF ICC IHC Flow IP
    Catalog Number:
    31105
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher rabbit anti goat igg hrp conjugated antibody
    The immunized mouse serum <t>IgG</t> (H+L) titer and IgG subtypes were detected using the indirect ELISA assay. (A) IgG titers of BALB/c mice immunized with rEF-Tu, rHSP70, Mo extracts and PBS sera of each groups were collected on days 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 DAI. (B) Determination of IgG subtypes in sera of the immunized mice. The sera of each group were collected at 35 DAI. ELISA plates were coated with purified rEF-Tu proteins or rHSP70 proteins or Mo extracts of M . ovipneumoniae wild strain Mo-1 at a concentration of 100 ng per well. Anti-mouse IgG (H+L) or IgG1 or IgG2a <t>HRP-conjugated</t> antibodies were used as secondary antibodies. Asterisks indicates the results of the One-Way ANOVA using the Tukey test, compared with the PBS negative control group, with P
    Rabbit anti Goat IgG H L Secondary Antibody for Western Blot IF ICC IHC Flow IP
    https://www.bioz.com/result/rabbit anti goat igg hrp conjugated antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti goat igg hrp conjugated antibody - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice"

    Article Title: Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0161170

    The immunized mouse serum IgG (H+L) titer and IgG subtypes were detected using the indirect ELISA assay. (A) IgG titers of BALB/c mice immunized with rEF-Tu, rHSP70, Mo extracts and PBS sera of each groups were collected on days 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 DAI. (B) Determination of IgG subtypes in sera of the immunized mice. The sera of each group were collected at 35 DAI. ELISA plates were coated with purified rEF-Tu proteins or rHSP70 proteins or Mo extracts of M . ovipneumoniae wild strain Mo-1 at a concentration of 100 ng per well. Anti-mouse IgG (H+L) or IgG1 or IgG2a HRP-conjugated antibodies were used as secondary antibodies. Asterisks indicates the results of the One-Way ANOVA using the Tukey test, compared with the PBS negative control group, with P
    Figure Legend Snippet: The immunized mouse serum IgG (H+L) titer and IgG subtypes were detected using the indirect ELISA assay. (A) IgG titers of BALB/c mice immunized with rEF-Tu, rHSP70, Mo extracts and PBS sera of each groups were collected on days 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 DAI. (B) Determination of IgG subtypes in sera of the immunized mice. The sera of each group were collected at 35 DAI. ELISA plates were coated with purified rEF-Tu proteins or rHSP70 proteins or Mo extracts of M . ovipneumoniae wild strain Mo-1 at a concentration of 100 ng per well. Anti-mouse IgG (H+L) or IgG1 or IgG2a HRP-conjugated antibodies were used as secondary antibodies. Asterisks indicates the results of the One-Way ANOVA using the Tukey test, compared with the PBS negative control group, with P

    Techniques Used: Indirect ELISA, Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification, Concentration Assay, Negative Control

    Related Articles

    Incubation:

    Article Title: A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis
    Article Snippet: The plate was then washed with PBS-T, and 50 μL of anti-RANKL antibody solution (0.5 μg/mL; AF626, R & D systems) was added to each well to detect RANKL bound to GM-0111. .. After 1 hr of incubation at room temperature, the plate was washed with PBS-T and 100 μL of horseradish peroxidase (HRP)-conjugated anti-goat IgG (ThermoScientific, cat# 31402) was added. .. After 1 hr of incubation at room temperature, the plate was washed with PBS-T, and the bound HRP was determined by incubating with 100 μL of 3,3′,5,5′-tetramethylbenzidine solution (50-77-18, KPL Inc., MD) as a substrate.

    Article Title: Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice
    Article Snippet: The membranes were blocked in PBST with 5% nonfat milk for 1h and then incubated overnight at 4°C with specific sheep M . ovipneumoniae hyper-immune sera. .. Following washing with PBST three times, membranes were incubated with rabbit anti-goat IgG HRP- conjugated antibody at 37°C for 1 h and exposed to SuperSignal® West Pico Chemiluminescent Substrate reagent for detection (Thermo Scientific, USA). ..

    Article Title: Identification and characterisation of the CD40-ligand of Sigmodon hispidus
    Article Snippet: Plates were washed with wash buffer and incubated at 37°C for 1 hour with 100μl/well goat anti-mouseCD40L (R & D Systems) 2ug/ml in blocking buffer. .. Plates were subsequently washed and incubated at 37°C for 1 hour with 100μl/well with rabbit anti-goat IgG HRP conjugate (Zymed). .. Plates were washed again and incubated for 10 min in the dark with 100μl/well 3,3'5,5'-tetramethylbenzidine substrate (New England Bio Labs).

    Protease Inhibitor:

    Article Title: Chromatoid Body Protein TDRD6 Supports Long 3’ UTR Triggered Nonsense Mediated mRNA Decay
    Article Snippet: .. 150 μg of protein extract diluted in IP buffer (50 mM Tris-HCl at pH 7.4 (Roth), 150 mM NaCl (Roth), 0.25% Triton-X100 (Sigma), 1 mM EDTA (Sigma), 5mM NaF (Sigma), 1mM Na2VO3 (Sigma), 1mM PMSF (Sigma), 1x protease inhibitor cocktail complete mini (Roche)) to a final volume of 250 μL and antibodies coupled to Dynabeads used for immunoprecipitation: goat polyclonal anti UPF1 (Bethyl), rabbit polyclonal anti MVH (Abcam), rabbit polyclonal anti TDRD6 (Antibody Verify), rabbit polyclonal anti UPF2 [ ], goat IgG (Invitrogen) and rabbit IgG (Invitrogen). ..

    Immunoprecipitation:

    Article Title: Chromatoid Body Protein TDRD6 Supports Long 3’ UTR Triggered Nonsense Mediated mRNA Decay
    Article Snippet: .. 150 μg of protein extract diluted in IP buffer (50 mM Tris-HCl at pH 7.4 (Roth), 150 mM NaCl (Roth), 0.25% Triton-X100 (Sigma), 1 mM EDTA (Sigma), 5mM NaF (Sigma), 1mM Na2VO3 (Sigma), 1mM PMSF (Sigma), 1x protease inhibitor cocktail complete mini (Roche)) to a final volume of 250 μL and antibodies coupled to Dynabeads used for immunoprecipitation: goat polyclonal anti UPF1 (Bethyl), rabbit polyclonal anti MVH (Abcam), rabbit polyclonal anti TDRD6 (Antibody Verify), rabbit polyclonal anti UPF2 [ ], goat IgG (Invitrogen) and rabbit IgG (Invitrogen). ..

    other:

    Article Title: MFR, a Putative Receptor Mediating the Fusion of Macrophages
    Article Snippet: Goat anti-glutathione S -transferase (GST) was obtained from Pharmacia (Uppsala, Sweden), and rabbit anti-goat IgG conjugated to HRP was obtained from Zymed (South San Francisco, Calif.).

    Imaging:

    Article Title: Quantifying Fluorescently Labeled Ceramide Levels in Human Sarcoma Cell Lines in Response to a Sphingomyelin Synthase Inhibitor
    Article Snippet: Materials C-6 NBD Ceramide (Toronto Research Chemicals, North York, ON, Canada; Cat. no.: N381205) Jaspine B (Pashikanti lab, Pocatello, ID, USA) SJSA-1 (ATCC, Manassas, VA, USA; Cat. no.: CRL-2098) U2-OS (ATCC, Manassas, VA, USA; Cat. no.: HTB-96) RPMI 1640 Medium (Caisson Labs, Smithfield, UT, USA; Cat. no.: RPL04-6) Fetal Bovine Serum (Atlanta Biologicals, Flowery Branch, GA, USA; Cat. no.: S11150) Penicillin/Streptomycin (Atlanta Biologicals, Flowery Branch, GA, USA; Cat. no.: B21110) Trypsin 0.25% EDTA (Atlanta Biologicals, Flowery Branch, GA, USA; Cat. no.: B81310) NaCl (Sigma Chemical Company, St. Louis, MO, USA; Cat. no.: S-9625) NP-40 (Fluka Analytical, Mexico City, Mexico; Cat. no.: 74385) Phosphate Buffered Saline (Genesee Scientific, El Cajon, CA, USA; Cat. no.: 25-508) Pyridine (Alfa Aesar, Haverhill, MA, USA; Cat. no.: A12005-AP) Silica gel plates TLC-G (Silicycle, Quebec, QC, Canada; Cat. no.: TLG-R10014BK-323) Toluene (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: S25611A) Tris HCl (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: EC 201-064-4) .. Equipment Azure c600 Imaging Station (Azure Biosystems, Dublin, CA, USA; Cat. no.: c600) Countess II (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: AMQAX1000) Countess Slides (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: C10283) MiniSpin (Eppendorf, Hamburg, Germany; Cat. no.: 22620100) Spectrafuge 6C (Labnet, Edison, NJ, USA; Cat. no.: LI-CF-SF6C) EVOS FL (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: AMF4300) FORMA SERIES II CO₂ Incubator (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: 31300) ..

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  • 97
    Thermo Fisher goat anti rabbit horseradish peroxidase conjugate
    Compound 7 inhibits the MIF–sCD74 binding as determined by an ELISA assay. Binding of MBP–sCD74 to MIF-coated ELISA plates was detected using a <t>rabbit</t> <t>anti-CD74</t> antibody as the primary and a <t>goat</t> anti-rabbit <t>horseradish</t> <t>peroxidase</t> <t>conjugate</t> as the secondary antibody. Data are displayed as mean ± SD ( n = 3).
    Goat Anti Rabbit Horseradish Peroxidase Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit horseradish peroxidase conjugate/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit horseradish peroxidase conjugate - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher rabbit anti goat igg hrp conjugated antibody
    The immunized mouse serum <t>IgG</t> (H+L) titer and IgG subtypes were detected using the indirect ELISA assay. (A) IgG titers of BALB/c mice immunized with rEF-Tu, rHSP70, Mo extracts and PBS sera of each groups were collected on days 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 DAI. (B) Determination of IgG subtypes in sera of the immunized mice. The sera of each group were collected at 35 DAI. ELISA plates were coated with purified rEF-Tu proteins or rHSP70 proteins or Mo extracts of M . ovipneumoniae wild strain Mo-1 at a concentration of 100 ng per well. Anti-mouse IgG (H+L) or IgG1 or IgG2a <t>HRP-conjugated</t> antibodies were used as secondary antibodies. Asterisks indicates the results of the One-Way ANOVA using the Tukey test, compared with the PBS negative control group, with P
    Rabbit Anti Goat Igg Hrp Conjugated Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti goat igg hrp conjugated antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti goat igg hrp conjugated antibody - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher horseradish peroxidase hrp conjugated rabbit antimouse igg
    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide <t>(HRP)</t> <t>antimouse</t> <t>IgG</t> as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.
    Horseradish Peroxidase Hrp Conjugated Rabbit Antimouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated rabbit antimouse igg/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated rabbit antimouse igg - by Bioz Stars, 2021-03
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    Image Search Results


    Compound 7 inhibits the MIF–sCD74 binding as determined by an ELISA assay. Binding of MBP–sCD74 to MIF-coated ELISA plates was detected using a rabbit anti-CD74 antibody as the primary and a goat anti-rabbit horseradish peroxidase conjugate as the secondary antibody. Data are displayed as mean ± SD ( n = 3).

    Journal: Journal of Medicinal Chemistry

    Article Title: 7-Hydroxycoumarins Are Affinity-Based Fluorescent Probes for Competitive Binding Studies of Macrophage Migration Inhibitory Factor

    doi: 10.1021/acs.jmedchem.0c01160

    Figure Lengend Snippet: Compound 7 inhibits the MIF–sCD74 binding as determined by an ELISA assay. Binding of MBP–sCD74 to MIF-coated ELISA plates was detected using a rabbit anti-CD74 antibody as the primary and a goat anti-rabbit horseradish peroxidase conjugate as the secondary antibody. Data are displayed as mean ± SD ( n = 3).

    Article Snippet: After washing, the wells were incubated with 100 μL of a rabbit anti-CD74 pAb solution (1:1000 dilution in PBS, 0.2% BSA) (Sinobiological, The Netherlands) at room temperature for 30 min. After removing the anti-CD74 solution and washing, a solution of 100 μL of goat anti-rabbit horseradish peroxidase conjugate (1:1000 dilution in PBS, 0.2% BSA) (Life Technologies, The Netherlands) was added and incubated at room temperature for 30 min. After washing, binding was visualized by conversion of 100 μL of aqueous tetramethylbenzydine (TMB) solution (Sigma Aldrich, The Netherlands), which was quenched with an aqueous 1 N H2 SO4 solution (100 μL).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

    The immunized mouse serum IgG (H+L) titer and IgG subtypes were detected using the indirect ELISA assay. (A) IgG titers of BALB/c mice immunized with rEF-Tu, rHSP70, Mo extracts and PBS sera of each groups were collected on days 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 DAI. (B) Determination of IgG subtypes in sera of the immunized mice. The sera of each group were collected at 35 DAI. ELISA plates were coated with purified rEF-Tu proteins or rHSP70 proteins or Mo extracts of M . ovipneumoniae wild strain Mo-1 at a concentration of 100 ng per well. Anti-mouse IgG (H+L) or IgG1 or IgG2a HRP-conjugated antibodies were used as secondary antibodies. Asterisks indicates the results of the One-Way ANOVA using the Tukey test, compared with the PBS negative control group, with P

    Journal: PLoS ONE

    Article Title: Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice

    doi: 10.1371/journal.pone.0161170

    Figure Lengend Snippet: The immunized mouse serum IgG (H+L) titer and IgG subtypes were detected using the indirect ELISA assay. (A) IgG titers of BALB/c mice immunized with rEF-Tu, rHSP70, Mo extracts and PBS sera of each groups were collected on days 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 DAI. (B) Determination of IgG subtypes in sera of the immunized mice. The sera of each group were collected at 35 DAI. ELISA plates were coated with purified rEF-Tu proteins or rHSP70 proteins or Mo extracts of M . ovipneumoniae wild strain Mo-1 at a concentration of 100 ng per well. Anti-mouse IgG (H+L) or IgG1 or IgG2a HRP-conjugated antibodies were used as secondary antibodies. Asterisks indicates the results of the One-Way ANOVA using the Tukey test, compared with the PBS negative control group, with P

    Article Snippet: Following washing with PBST three times, membranes were incubated with rabbit anti-goat IgG HRP- conjugated antibody at 37°C for 1 h and exposed to SuperSignal® West Pico Chemiluminescent Substrate reagent for detection (Thermo Scientific, USA).

    Techniques: Indirect ELISA, Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification, Concentration Assay, Negative Control

    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.

    Journal: Molecular Vision

    Article Title: Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens

    doi:

    Figure Lengend Snippet: The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit antimouse IgG and goat antihuman IgG were obtained from Zymed Laboratories (San Francisco, CA).

    Techniques: Mouse Assay, Western Blot, Enzyme-linked Immunosorbent Assay, SDS Page, Incubation, Binding Assay, Staining, Molecular Weight, Marker

    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Signal-dependent Slow Leukocyte Rolling Does Not Require Cytoskeletal Anchorage of P-selectin Glycoprotein Ligand-1 (PSGL-1) or Integrin ?L?2 *

    doi: 10.1074/jbc.M112.361519

    Figure Lengend Snippet: Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Article Snippet: Murine anti-moesin mAb (clone 38/87) and horseradish peroxidase-conjugated goat anti-murine IgG were from Thermo Scientific (Fremont, CA).

    Techniques: Incubation, Derivative Assay, Flow Cytometry, Cytometry