rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh  (Millipore)


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    Structured Review

    Millipore rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh
    Rabbit Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier
    Article Snippet: Green, Northwestern University, Evanston, IL), rabbit anti-Dsg2 (clone EPR6767), anti-MMP2 (Novus Biologicals, Littleton, CO), anti-Dsg2 (clone EPR6768), mouse anti-His (clone HIS.H8; Abcam, Cambridge, MA), guinea pig anti-Dsc2 (American Research Products, Waltham, MA), mouse anti-Dsc2/3 (clone 7G6; Life Technologies, Carlsbad, CA), anti–E-cadherin (clone HECD-1, a generous gift from A. S. Yap, University of Queensland, Brisbane St Lucia, Australia; ), anti–E-cadherin (clone 36; BD Biosciences, San Jose, CA), rat anti–uvomorulin/E-cadherin (clone DECMA-1), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-actin (Sigma-Aldrich, St. Louis, MO), mouse anti-JAM-A (clone J10.4, generated in-house; ), anti-SIRPα (clone SAF17.2, generated in-house; ), anti–Myc-tag (clone 9B11), rabbit anti–phospho-EGFR (Tyr-1068), anti-EGFR, anti–phospho-HER2 (Tyr-1196), anti-HER2, anti–phospho-HER3 (Tyr-1289), anti-HER3, anti–phospho-Akt (Thr-308), anti–phospho-Akt (Ser-473), anti–pan Akt, anti–phospho-S6 ribosomal protein (Ser-235/Ser-236), anti–S6 ribosomal protein, anti–phospho-Erk1/2 (Thr-202/Tyr-204), anti-Erk1/2, and anti-MMP9 (Cell Signaling Technology, Danvers, MA). .. Recombinant human IFN-γ, TNF-α, mouse IL-1β, IFN-γ, and TNF-α were purchased from PeproTech (Rocky Hill, NJ).

    Article Title: Intracellular Desmoglein-2 cleavage sensitizes epithelial cells to apoptosis in response to pro-inflammatory cytokines
    Article Snippet: Green, Northwestern University, Evanston, IL), mouse anti-beta-actin (clone AC-74) [1:5000-1:10,000], rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [1:20,000], mouse anti-tubulin (clone DM1A [1:10,000]; Sigma-Aldrich; St. Louis, MO, USA), rabbit anti-cleaved PARP (clone D64E10 XP(r)) [1:1000], rabbit anti-PARP [1:1000], rabbit anti-Bcl-XL (clone 54H6) [1:2000], rabbit anti-cleaved Notch (clone D3B8) [1:1000], rabbit anti-cytochrome c (clone D18C7) [1:1000], mouse anti-caspase-8 (clone 1C12), mouse anti-Myc tag (clone 9B11; Cell Signaling Technology; Danvers, CT, USA) [1:5000], rabbit anti-Na/K ATPase [1:100,000] (clone EP1845Y; Abcam; Cambridge, MA, USA), rabbit anti-Mcl1 [1:3000] (Enzo Life Sciences; Farmingdale, NY, USA), Armenian hamster anti-Bcl-2 [1:125] (BD Biosceicnes; San Jose, CA, USA), rabbit anti-bovine serum albumin (BSA) (clone B-140) [1:10,000], rat anti-guanylate binding protein-1 (GBP-1 (clone 1B1) [1:750]; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Recombinant human TNF-α, IFN-γ, and TRAIL were purchased from PeproTech (Rocky Hill, NJ, USA).

    Activation Assay:

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis
    Article Snippet: .. Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-AktSer473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls. .. Following primary antibody incubation, the membranes were washed 3x with PBST and incubated with either Li-Cor IRdye 600 or 800 for imaging on a Li-Cor Odyssey Fc machine, or horseradish peroxidase (HRP)-conjugated secondary antibodies for use in enhanced chemiluminescence (ECL) imaging using a Li-Cor c-DiGit imaging system (Li-Cor).

    Generated:

    Article Title: Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier
    Article Snippet: .. Green, Northwestern University, Evanston, IL), rabbit anti-Dsg2 (clone EPR6767), anti-MMP2 (Novus Biologicals, Littleton, CO), anti-Dsg2 (clone EPR6768), mouse anti-His (clone HIS.H8; Abcam, Cambridge, MA), guinea pig anti-Dsc2 (American Research Products, Waltham, MA), mouse anti-Dsc2/3 (clone 7G6; Life Technologies, Carlsbad, CA), anti–E-cadherin (clone HECD-1, a generous gift from A. S. Yap, University of Queensland, Brisbane St Lucia, Australia; ), anti–E-cadherin (clone 36; BD Biosciences, San Jose, CA), rat anti–uvomorulin/E-cadherin (clone DECMA-1), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-actin (Sigma-Aldrich, St. Louis, MO), mouse anti-JAM-A (clone J10.4, generated in-house; ), anti-SIRPα (clone SAF17.2, generated in-house; ), anti–Myc-tag (clone 9B11), rabbit anti–phospho-EGFR (Tyr-1068), anti-EGFR, anti–phospho-HER2 (Tyr-1196), anti-HER2, anti–phospho-HER3 (Tyr-1289), anti-HER3, anti–phospho-Akt (Thr-308), anti–phospho-Akt (Ser-473), anti–pan Akt, anti–phospho-S6 ribosomal protein (Ser-235/Ser-236), anti–S6 ribosomal protein, anti–phospho-Erk1/2 (Thr-202/Tyr-204), anti-Erk1/2, and anti-MMP9 (Cell Signaling Technology, Danvers, MA). .. Fluorophore-conjugated secondary antibodies (Alexa dye series) were obtained from Life Technologies.

    Article Title: Intracellular Desmoglein-2 cleavage sensitizes epithelial cells to apoptosis in response to pro-inflammatory cytokines
    Article Snippet: Antibodies and reagents The following primary monoclonal and polyclonal antibodies were used to detect proteins by immunoblot analysis: mouse anti-Dsg2 {extracellular} (clone AH12.2, generated in-house , [1:1000], mouse anti-Dsg2 {intracellular} [1:750] (clone 4B2; a kind gift from Dr. K.J. .. Green, Northwestern University, Evanston, IL), mouse anti-beta-actin (clone AC-74) [1:5000-1:10,000], rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [1:20,000], mouse anti-tubulin (clone DM1A [1:10,000]; Sigma-Aldrich; St. Louis, MO, USA), rabbit anti-cleaved PARP (clone D64E10 XP(r)) [1:1000], rabbit anti-PARP [1:1000], rabbit anti-Bcl-XL (clone 54H6) [1:2000], rabbit anti-cleaved Notch (clone D3B8) [1:1000], rabbit anti-cytochrome c (clone D18C7) [1:1000], mouse anti-caspase-8 (clone 1C12), mouse anti-Myc tag (clone 9B11; Cell Signaling Technology; Danvers, CT, USA) [1:5000], rabbit anti-Na/K ATPase [1:100,000] (clone EP1845Y; Abcam; Cambridge, MA, USA), rabbit anti-Mcl1 [1:3000] (Enzo Life Sciences; Farmingdale, NY, USA), Armenian hamster anti-Bcl-2 [1:125] (BD Biosceicnes; San Jose, CA, USA), rabbit anti-bovine serum albumin (BSA) (clone B-140) [1:10,000], rat anti-guanylate binding protein-1 (GBP-1 (clone 1B1) [1:750]; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Microscopy:

    Article Title: Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier
    Article Snippet: Antibodies and reagents The following primary monoclonal and polyclonal antibodies were used to detect proteins by immunofluorescence microscopy and/or immunoblot analysis: mouse anti-Dsg2 (clone AH12.2, generated in-house; ; ), anti-Dsg2 (clone 4B2, a kind gift from K. J. .. Green, Northwestern University, Evanston, IL), rabbit anti-Dsg2 (clone EPR6767), anti-MMP2 (Novus Biologicals, Littleton, CO), anti-Dsg2 (clone EPR6768), mouse anti-His (clone HIS.H8; Abcam, Cambridge, MA), guinea pig anti-Dsc2 (American Research Products, Waltham, MA), mouse anti-Dsc2/3 (clone 7G6; Life Technologies, Carlsbad, CA), anti–E-cadherin (clone HECD-1, a generous gift from A. S. Yap, University of Queensland, Brisbane St Lucia, Australia; ), anti–E-cadherin (clone 36; BD Biosciences, San Jose, CA), rat anti–uvomorulin/E-cadherin (clone DECMA-1), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-actin (Sigma-Aldrich, St. Louis, MO), mouse anti-JAM-A (clone J10.4, generated in-house; ), anti-SIRPα (clone SAF17.2, generated in-house; ), anti–Myc-tag (clone 9B11), rabbit anti–phospho-EGFR (Tyr-1068), anti-EGFR, anti–phospho-HER2 (Tyr-1196), anti-HER2, anti–phospho-HER3 (Tyr-1289), anti-HER3, anti–phospho-Akt (Thr-308), anti–phospho-Akt (Ser-473), anti–pan Akt, anti–phospho-S6 ribosomal protein (Ser-235/Ser-236), anti–S6 ribosomal protein, anti–phospho-Erk1/2 (Thr-202/Tyr-204), anti-Erk1/2, and anti-MMP9 (Cell Signaling Technology, Danvers, MA).

    Concentration Assay:

    Article Title: Intracellular Desmoglein-2 cleavage sensitizes epithelial cells to apoptosis in response to pro-inflammatory cytokines
    Article Snippet: Green, Northwestern University, Evanston, IL), mouse anti-beta-actin (clone AC-74) [1:5000-1:10,000], rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [1:20,000], mouse anti-tubulin (clone DM1A [1:10,000]; Sigma-Aldrich; St. Louis, MO, USA), rabbit anti-cleaved PARP (clone D64E10 XP(r)) [1:1000], rabbit anti-PARP [1:1000], rabbit anti-Bcl-XL (clone 54H6) [1:2000], rabbit anti-cleaved Notch (clone D3B8) [1:1000], rabbit anti-cytochrome c (clone D18C7) [1:1000], mouse anti-caspase-8 (clone 1C12), mouse anti-Myc tag (clone 9B11; Cell Signaling Technology; Danvers, CT, USA) [1:5000], rabbit anti-Na/K ATPase [1:100,000] (clone EP1845Y; Abcam; Cambridge, MA, USA), rabbit anti-Mcl1 [1:3000] (Enzo Life Sciences; Farmingdale, NY, USA), Armenian hamster anti-Bcl-2 [1:125] (BD Biosceicnes; San Jose, CA, USA), rabbit anti-bovine serum albumin (BSA) (clone B-140) [1:10,000], rat anti-guanylate binding protein-1 (GBP-1 (clone 1B1) [1:750]; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Goat anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA) and used at a concentration of 1:10,000.

    Incubation:

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis
    Article Snippet: .. Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-AktSer473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls. .. Following primary antibody incubation, the membranes were washed 3x with PBST and incubated with either Li-Cor IRdye 600 or 800 for imaging on a Li-Cor Odyssey Fc machine, or horseradish peroxidase (HRP)-conjugated secondary antibodies for use in enhanced chemiluminescence (ECL) imaging using a Li-Cor c-DiGit imaging system (Li-Cor).

    Blocking Assay:

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis
    Article Snippet: .. Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-AktSer473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls. .. Following primary antibody incubation, the membranes were washed 3x with PBST and incubated with either Li-Cor IRdye 600 or 800 for imaging on a Li-Cor Odyssey Fc machine, or horseradish peroxidase (HRP)-conjugated secondary antibodies for use in enhanced chemiluminescence (ECL) imaging using a Li-Cor c-DiGit imaging system (Li-Cor).

    Marker:

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis
    Article Snippet: .. Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-AktSer473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls. .. Following primary antibody incubation, the membranes were washed 3x with PBST and incubated with either Li-Cor IRdye 600 or 800 for imaging on a Li-Cor Odyssey Fc machine, or horseradish peroxidase (HRP)-conjugated secondary antibodies for use in enhanced chemiluminescence (ECL) imaging using a Li-Cor c-DiGit imaging system (Li-Cor).

    Western Blot:

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis
    Article Snippet: Paragraph title: Western blot ... Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-AktSer473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls.

    Binding Assay:

    Article Title: Intracellular Desmoglein-2 cleavage sensitizes epithelial cells to apoptosis in response to pro-inflammatory cytokines
    Article Snippet: .. Green, Northwestern University, Evanston, IL), mouse anti-beta-actin (clone AC-74) [1:5000-1:10,000], rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [1:20,000], mouse anti-tubulin (clone DM1A [1:10,000]; Sigma-Aldrich; St. Louis, MO, USA), rabbit anti-cleaved PARP (clone D64E10 XP(r)) [1:1000], rabbit anti-PARP [1:1000], rabbit anti-Bcl-XL (clone 54H6) [1:2000], rabbit anti-cleaved Notch (clone D3B8) [1:1000], rabbit anti-cytochrome c (clone D18C7) [1:1000], mouse anti-caspase-8 (clone 1C12), mouse anti-Myc tag (clone 9B11; Cell Signaling Technology; Danvers, CT, USA) [1:5000], rabbit anti-Na/K ATPase [1:100,000] (clone EP1845Y; Abcam; Cambridge, MA, USA), rabbit anti-Mcl1 [1:3000] (Enzo Life Sciences; Farmingdale, NY, USA), Armenian hamster anti-Bcl-2 [1:125] (BD Biosceicnes; San Jose, CA, USA), rabbit anti-bovine serum albumin (BSA) (clone B-140) [1:10,000], rat anti-guanylate binding protein-1 (GBP-1 (clone 1B1) [1:750]; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Goat anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA) and used at a concentration of 1:10,000.

    Immunofluorescence:

    Article Title: Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier
    Article Snippet: Antibodies and reagents The following primary monoclonal and polyclonal antibodies were used to detect proteins by immunofluorescence microscopy and/or immunoblot analysis: mouse anti-Dsg2 (clone AH12.2, generated in-house; ; ), anti-Dsg2 (clone 4B2, a kind gift from K. J. .. Green, Northwestern University, Evanston, IL), rabbit anti-Dsg2 (clone EPR6767), anti-MMP2 (Novus Biologicals, Littleton, CO), anti-Dsg2 (clone EPR6768), mouse anti-His (clone HIS.H8; Abcam, Cambridge, MA), guinea pig anti-Dsc2 (American Research Products, Waltham, MA), mouse anti-Dsc2/3 (clone 7G6; Life Technologies, Carlsbad, CA), anti–E-cadherin (clone HECD-1, a generous gift from A. S. Yap, University of Queensland, Brisbane St Lucia, Australia; ), anti–E-cadherin (clone 36; BD Biosciences, San Jose, CA), rat anti–uvomorulin/E-cadherin (clone DECMA-1), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-actin (Sigma-Aldrich, St. Louis, MO), mouse anti-JAM-A (clone J10.4, generated in-house; ), anti-SIRPα (clone SAF17.2, generated in-house; ), anti–Myc-tag (clone 9B11), rabbit anti–phospho-EGFR (Tyr-1068), anti-EGFR, anti–phospho-HER2 (Tyr-1196), anti-HER2, anti–phospho-HER3 (Tyr-1289), anti-HER3, anti–phospho-Akt (Thr-308), anti–phospho-Akt (Ser-473), anti–pan Akt, anti–phospho-S6 ribosomal protein (Ser-235/Ser-236), anti–S6 ribosomal protein, anti–phospho-Erk1/2 (Thr-202/Tyr-204), anti-Erk1/2, and anti-MMP9 (Cell Signaling Technology, Danvers, MA).

    Imaging:

    Article Title: Carboxyl-terminal modulator protein regulates Akt signaling during skeletal muscle atrophy in vitro and a mouse model of amyotrophic lateral sclerosis
    Article Snippet: Then, the membranes were incubated with the following primary antibodies in PBS + 0.1% Tween 20 (PBST) + milk blocking buffer (ThermoPierce) overnight at 4 °C or 1 hr at room temperature: mouse anti-pan Akt, rabbit anti-p-AktSer473 , rabbit anti-LAMP1 (1:1,000), a marker for lysosomal activation, TNFα (1:1,000), rabbit anti-CTMP (1:1,000) (Cell Signaling Inc.), rabbit anti-LC3 (1:500; Abcam), a marker of autophagosome flux, rabbit anti-MURF-1, a marker of atrophy (1:1000, EMC Biosciences), and mouse anti-β-tubulin (Cell Signaling Inc.) and rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich) were used as loading controls. .. Following primary antibody incubation, the membranes were washed 3x with PBST and incubated with either Li-Cor IRdye 600 or 800 for imaging on a Li-Cor Odyssey Fc machine, or horseradish peroxidase (HRP)-conjugated secondary antibodies for use in enhanced chemiluminescence (ECL) imaging using a Li-Cor c-DiGit imaging system (Li-Cor).

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  • 90
    Millipore rabbit anti bax
    Effects of coffee oil-algae oil nanoemulsions on protein expressions of <t>cyclin</t> B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and <t>Bax,</t> Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p
    Rabbit Anti Bax, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax/product/Millipore
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    91
    Millipore rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to <t>glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH),</t> which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.
    Rabbit Polyclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

    doi: 10.2147/IJN.S144705

    Figure Lengend Snippet: Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Article Snippet: The primary antibodies include mouse monoclonal anti-α-tubulin antibody (Sigma–Aldrich Co.), mouse anti-cyclin A and rabbit anti-Bax (EMD Millipore), mouse anti-CDK2, mouse anti-cytochrome C, mouse anti-p21, mouse anti-cyclin B, and mouse anti-Bcl-2 (BD Biosciences), as well as anti-cdc2 (CDK1) and mouse anti-p53 (Novus Biologicals Co., Littleton, CO, USA).

    Techniques: Incubation, Standard Deviation

    L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Journal: BMC Medicine

    Article Title: Sodium vanadate combined with l-ascorbic acid delays disease progression, enhances motor performance, and ameliorates muscle atrophy and weakness in mice with spinal muscular atrophy

    doi: 10.1186/1741-7015-11-38

    Figure Lengend Snippet: L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Article Snippet: Primary antibodies used for western blotting included mouse anti-SMN (1:5,000; BD Biosciences, San Diego, CA, USA), mouse anti-β-actin (1:10,000; Sigma), rabbit anti-Bax (1:1,000; Millipore, Temecula, CA, USA), and rabbit anti-caspase 3 (1:1,000; Cell Signaling, Temecula, CA, USA).

    Techniques: Western Blot, Expressing

    Involvement of FLIP and p53 signaling pathways in the regulation of apoptosis in avian H9N2–infected HBE1 and TBE cells. Cytosolic ( C ) and nuclear ( N ) fractions of cell lysates were prepared from uninfected and infected cells at 12 hours ( A and B ) after infection or as indicated, and fractionated on 12–15% SDS-PAGE. Proteins were transferred to PDVF membranes before they were blotted with specific antibodies. Each analysis was performed at least three times, and a representative result is presented. ( A ) FLIP was downregulated in infected cells. ( B ) Bcl-2 family members Bax and Bcl-x s were upregulated in infected cells. ( C ) p53 was phosphorylated at serine 392 in H9N2-infected cells. Arrows indicate phosphorylated p53 bands. *Nonspecific bands, shown in both infected and noninfected cells by the antibody.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Host Immune and Apoptotic Responses to Avian Influenza Virus H9N2 in Human Tracheobronchial Epithelial Cells

    doi: 10.1165/rcmb.2009-0120OC

    Figure Lengend Snippet: Involvement of FLIP and p53 signaling pathways in the regulation of apoptosis in avian H9N2–infected HBE1 and TBE cells. Cytosolic ( C ) and nuclear ( N ) fractions of cell lysates were prepared from uninfected and infected cells at 12 hours ( A and B ) after infection or as indicated, and fractionated on 12–15% SDS-PAGE. Proteins were transferred to PDVF membranes before they were blotted with specific antibodies. Each analysis was performed at least three times, and a representative result is presented. ( A ) FLIP was downregulated in infected cells. ( B ) Bcl-2 family members Bax and Bcl-x s were upregulated in infected cells. ( C ) p53 was phosphorylated at serine 392 in H9N2-infected cells. Arrows indicate phosphorylated p53 bands. *Nonspecific bands, shown in both infected and noninfected cells by the antibody.

    Article Snippet: Anti-Bax rabbit (Ab-1), anti-Bcl-xs rabbit (Ab-1), and anti-Bak monoclonal (Ab-1) antibodies were purchased from EMD Biosciences (Gibbstown, NJ).

    Techniques: Infection, SDS Page

    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Journal: Acta Physiologica (Oxford, England)

    Article Title: Impaired structural and functional regeneration of skeletal muscles from β2-adrenoceptor knockout mice

    doi: 10.1111/apha.12329

    Figure Lengend Snippet: Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Article Snippet: Antibodies for Western blotting The primary antibodies used for Western blotting were (1) rabbit polyclonal anti-Smad4 (1 : 1000; cat# 9515; Cell Signaling Technology), (2) rabbit polyclonal anti-akirin1 (1 : 1000; cat# ab77075; Abcam) and (3) rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1 : 2000; cat# ABS16; Millipore, Temecula, CA, USA).

    Techniques: Expressing, Western Blot, Mouse Assay, SDS Page, Molecular Weight