rabbit anti glial fibrillary acidic protein gfap  (Boster Bio)


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    Name:
    Anti GFAP Rabbit Monoclonal Antibody
    Description:

    Catalog Number:
    M00213-1
    Price:
    299.0
    Category:
    Primary Antibodies
    Reactivity:
    Human Mouse Rat
    Applications:
    IP, IF, IHC, ICC, WB
    Immunogen:
    A synthesized peptide derived from human GFAP
    Host:
    Rabbit
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    Structured Review

    Boster Bio rabbit anti glial fibrillary acidic protein gfap
    Anti GFAP Rabbit Monoclonal Antibody

    https://www.bioz.com/result/rabbit anti glial fibrillary acidic protein gfap/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glial fibrillary acidic protein gfap - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression"

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.244794

    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.
    Figure Legend Snippet: Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Techniques Used: Light Microscopy

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    Incubation:

    Article Title: Clobetasol propionate enhances neural stem cell and oligodendrocyte differentiation
    Article Snippet: .. Cells were incubated with the following primary antibodies: Anti-growth associated protein 43 (GAP-43; rabbit polyclonal; 1:200; cat. no. PA-1037), anti-myelin basic protein (MBP; rabbit polyclonal; 1:200; cat. no. BA0094), anti-nestin (1:200; cat. no. PB0920), anti-glial fibrillary acidic protein (GFAP; mouse monoclonal; 1:200; cat. no. PB0046; all Boster Biological Technology), anti-SHH (1:200; rabbit monoclonal; cat. no. ab53281; Abcam), anti-phosphorylated adenosine 5-monophosphate (p-AMP)-activated protein kinase (p-AMPK; rabbit polyclonal; 1:200; cat. no. sc-25792) and anti-phosphorylated mitogen-activated protein kinase (p-ERK; rabbit polyclonal; 1:200; cat. no. sc-514302; both Santa Cruz Biotechnology, Inc.) for 12 h at 4°C. ..

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression
    Article Snippet: .. The neurons were incubated with rabbit anti-NSE (Boster) and mouse anti-β-tubulin (Proteintech) antibodies, while the astrocytes were incubated with rabbit anti-GFAP (Boster) antibody at 4°C overnight. ..

    Article Title: Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model
    Article Snippet: .. Next, the sections were incubated with rabbit anti-tyrosine hydroxylase (TH) antibody (1:100; Cat# ab75875; Abcam, Cambridge, UK) or rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:100; Cat# PB0046; Boster Biotechnology) at 4°C overnight. ..

    Article Title: A rat model for studying neural stem cell transplantation
    Article Snippet: .. The specimens were incubated overnight at 4 °C with the primary antibodies at the following dilutions: mouse anti-nestin 1:100 (BD Pharmingen, USA); mouse anti-NSE 1:10 (Chemicon, USA); rabbit anti-GFAP 1:100 (Boster, China). ..

    Labeling:

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression
    Article Snippet: .. Astrocytes and brain microvascular endothelial cells were labeled with rabbit anti-glial fibrillary acidic protein (GFAP) (1:100; Boster) and rabbit anti-PECAM-1/CD31 (1:100; Boster) antibodies. ..

    Staining:

    Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
    Article Snippet: .. The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or rabbit anti-rats GFAP antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) at 37°C. ..

    Marker:

    Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
    Article Snippet: .. The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or rabbit anti-rats GFAP antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) at 37°C. ..

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    Boster Bio rabbit anti glial fibrillary acidic protein gfap
    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and <t>microvascular</t> endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express <t>GFAP</t> (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.
    Rabbit Anti Glial Fibrillary Acidic Protein Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti glial fibrillary acidic protein gfap/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glial fibrillary acidic protein gfap - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    95
    Boster Bio rabbit anti gfap
    EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of <t>C3</t> in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), <t>GFAP</t> (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.
    Rabbit Anti Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gfap/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gfap - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Journal: Neural Regeneration Research

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression

    doi: 10.4103/1673-5374.244794

    Figure Lengend Snippet: Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Article Snippet: Astrocytes and brain microvascular endothelial cells were labeled with rabbit anti-glial fibrillary acidic protein (GFAP) (1:100; Boster) and rabbit anti-PECAM-1/CD31 (1:100; Boster) antibodies.

    Techniques: Light Microscopy

    Distribution of transplanted cells in the ischemic core of the brain on day 32 after transplantation in rats subjected to MCAO . Green labeling indicates BrdU-positive cells. DAPI (blue) labels nuclei. Immunofluorescent staining showing BrdU-positive transplanted cells (green) and NSE-positive cells (or GFAP-positive cells) (red). BrdU/NSE-positive double-labeled cells (or BrdU/GFAP-positive double-labeled cells) (yellow) (A,B) BMSC-N group; (C,D) BMSC group. The number of BrdU/GFAP-positive double-labeled cells or BrdU/NSE-positive double-labeled cells in the BMSC-N group was higher than that in the BMSC group. The BMSC-N group showed more BrdU/NSE-positive double-labeled cells than BrdU/GFAP-positive double-labeled cells. Conversely, the BMSC group had more BrdU/GFAP-positive double-labeled cells than BrdU/NSE-positive double-labeled cells. Significance levels are as shown in Table 3 . Scale bar = 100 μm.

    Journal: Frontiers in Neurology

    Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

    doi: 10.3389/fneur.2016.00183

    Figure Lengend Snippet: Distribution of transplanted cells in the ischemic core of the brain on day 32 after transplantation in rats subjected to MCAO . Green labeling indicates BrdU-positive cells. DAPI (blue) labels nuclei. Immunofluorescent staining showing BrdU-positive transplanted cells (green) and NSE-positive cells (or GFAP-positive cells) (red). BrdU/NSE-positive double-labeled cells (or BrdU/GFAP-positive double-labeled cells) (yellow) (A,B) BMSC-N group; (C,D) BMSC group. The number of BrdU/GFAP-positive double-labeled cells or BrdU/NSE-positive double-labeled cells in the BMSC-N group was higher than that in the BMSC group. The BMSC-N group showed more BrdU/NSE-positive double-labeled cells than BrdU/GFAP-positive double-labeled cells. Conversely, the BMSC group had more BrdU/GFAP-positive double-labeled cells than BrdU/NSE-positive double-labeled cells. Significance levels are as shown in Table 3 . Scale bar = 100 μm.

    Article Snippet: The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or rabbit anti-rats GFAP antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) at 37°C.

    Techniques: Transplantation Assay, Labeling, Staining

    Characteristics of hCSF-induced rat BMSC-differentiated neuron-like cells . (A,B) show post-induction by hCSF at day 1 and 4 (40×). Scale bar = 100 μm. (C) The protein levels were measured by a GELDOC instrument and normalized with respect to β-actin, which was chosen as an internal control. Each experiment was repeated at least three times. Variations in protein expression are given as arbitrary units. The expression of NES in cells induced by hCSF (0.43 ± 0.07) was significantly higher than that of P3-generation BMSCs (0.09 ± 0.03); meanwhile, GFAP also had small amount of expression (0.09 ± 0.02). However, P3-generation of BMSCs almost did not express GFAP (0 ± 0). (D–F) show Cells were positive for both NSE and GFAP, but expression levels for NSE were higher than GFAP. Western blot analysis of NSE and GFAP protein expression (200×). Scale bar = 100 μm.

    Journal: Frontiers in Neurology

    Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

    doi: 10.3389/fneur.2016.00183

    Figure Lengend Snippet: Characteristics of hCSF-induced rat BMSC-differentiated neuron-like cells . (A,B) show post-induction by hCSF at day 1 and 4 (40×). Scale bar = 100 μm. (C) The protein levels were measured by a GELDOC instrument and normalized with respect to β-actin, which was chosen as an internal control. Each experiment was repeated at least three times. Variations in protein expression are given as arbitrary units. The expression of NES in cells induced by hCSF (0.43 ± 0.07) was significantly higher than that of P3-generation BMSCs (0.09 ± 0.03); meanwhile, GFAP also had small amount of expression (0.09 ± 0.02). However, P3-generation of BMSCs almost did not express GFAP (0 ± 0). (D–F) show Cells were positive for both NSE and GFAP, but expression levels for NSE were higher than GFAP. Western blot analysis of NSE and GFAP protein expression (200×). Scale bar = 100 μm.

    Article Snippet: The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or rabbit anti-rats GFAP antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) at 37°C.

    Techniques: Expressing, Western Blot

    EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of C3 in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Electro-acupuncture Alleviates METH Withdrawal-induced Spatial Memory Deficits by Restoring Astrocyte-drived Glutamate Uptake in dCA1

    doi: 10.1101/2020.05.20.106153

    Figure Lengend Snippet: EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of C3 in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.

    Article Snippet: The following primary antibodies were used in these experiments: rat anti-C3 (1:200; abcam, UK), mouse anti-S100A10 (1:200; Thermo Fisher Scientific, USA), rabbit anti-GFAP (1:300; Boster, China), rat anti-GFAP(1:500, Thermo Fisher Scientific, USA), rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300; Thermo Fisher Scientific, USA), mouse anti-GS (1:500; abcam, UK), rabbit anti-c-Fos (1:2000; Cell Signaling Technology, USA), rabbit anti-SYP1 (Synaptotagmin-1, 1;500; Signalway Antibody, USA), mouse anti-VGLUT-1 (1;500; 1:200; abcam, UK), rabbit anti-NeuN (1:100; MERCK, Germany).

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Western Blot

    A1-like astrocytes showed decreased capacity of Glu uptake. A, Glu clearance in primary cultured astrocytes. (A1), Representative micrographs (left) and quantification (right) for GFAP staining in primary astrocytes. (A2), Top panels: immunohistochemistry micrographs for C3 (green), GFAP (red) and DAPI (blue) of control and A1 group. Bottom panels: left, ratio of C3 positive astrocytes; right, mRNA expression levels of C3. (A3), Ratio of Glu clearance after 1h application of Glu (100μM) in the control and A1 group. B, GLT-1, GLAST and GS expression in the primary cultured astrocytes. (B1) , Immunohistochemistry micrographs (left, GLT-1/GLAST/GS-red; C3-green and DAPI-blue) and quantification (right) for GLT-1, GS and GLAST of control and A1 group. (B2), Western blots (top) and quantification (bottom) of GLT-1, GS and GLAST protein levels of control and A1 group. Data are Mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Electro-acupuncture Alleviates METH Withdrawal-induced Spatial Memory Deficits by Restoring Astrocyte-drived Glutamate Uptake in dCA1

    doi: 10.1101/2020.05.20.106153

    Figure Lengend Snippet: A1-like astrocytes showed decreased capacity of Glu uptake. A, Glu clearance in primary cultured astrocytes. (A1), Representative micrographs (left) and quantification (right) for GFAP staining in primary astrocytes. (A2), Top panels: immunohistochemistry micrographs for C3 (green), GFAP (red) and DAPI (blue) of control and A1 group. Bottom panels: left, ratio of C3 positive astrocytes; right, mRNA expression levels of C3. (A3), Ratio of Glu clearance after 1h application of Glu (100μM) in the control and A1 group. B, GLT-1, GLAST and GS expression in the primary cultured astrocytes. (B1) , Immunohistochemistry micrographs (left, GLT-1/GLAST/GS-red; C3-green and DAPI-blue) and quantification (right) for GLT-1, GS and GLAST of control and A1 group. (B2), Western blots (top) and quantification (bottom) of GLT-1, GS and GLAST protein levels of control and A1 group. Data are Mean ± S.E.M.

    Article Snippet: The following primary antibodies were used in these experiments: rat anti-C3 (1:200; abcam, UK), mouse anti-S100A10 (1:200; Thermo Fisher Scientific, USA), rabbit anti-GFAP (1:300; Boster, China), rat anti-GFAP(1:500, Thermo Fisher Scientific, USA), rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300; Thermo Fisher Scientific, USA), mouse anti-GS (1:500; abcam, UK), rabbit anti-c-Fos (1:2000; Cell Signaling Technology, USA), rabbit anti-SYP1 (Synaptotagmin-1, 1;500; Signalway Antibody, USA), mouse anti-VGLUT-1 (1;500; 1:200; abcam, UK), rabbit anti-NeuN (1:100; MERCK, Germany).

    Techniques: Cell Culture, Staining, Immunohistochemistry, Expressing, Western Blot

    Graphene oxide inhibits the cell viability and promotes the differentiation of U251 GSCs. a U251 cells were cultured in a serum-free environment. Sphere morphology was photographed using light microscopy. Scale bar = 100 μm. b The expression of SOX2, CD133 and OCT4 in glioblastoma stem-like cells was increased during different periods. c Morphological appearance of U251 GSCs with or without treatment with GO for 2 days. The GSC spheres treated with GO showed adherent growth. Scale bar = 100 μm. d An MTT assay showed the cell viability of U251 GSCs with or without treatment with different dosages of GO for 2, 4, and 6 days. e Quantification of the mRNA levels of the stem cell markers SOX2 and differentiation markers (GFAP and TUJ1) in U251 GSCs with or without treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Journal: Journal of Translational Medicine

    Article Title: Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms

    doi: 10.1186/s12967-020-02359-z

    Figure Lengend Snippet: Graphene oxide inhibits the cell viability and promotes the differentiation of U251 GSCs. a U251 cells were cultured in a serum-free environment. Sphere morphology was photographed using light microscopy. Scale bar = 100 μm. b The expression of SOX2, CD133 and OCT4 in glioblastoma stem-like cells was increased during different periods. c Morphological appearance of U251 GSCs with or without treatment with GO for 2 days. The GSC spheres treated with GO showed adherent growth. Scale bar = 100 μm. d An MTT assay showed the cell viability of U251 GSCs with or without treatment with different dosages of GO for 2, 4, and 6 days. e Quantification of the mRNA levels of the stem cell markers SOX2 and differentiation markers (GFAP and TUJ1) in U251 GSCs with or without treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Article Snippet: We performed incubation with anti-CD133 (1:200; PB0168, Boster) and anti-GFAP (1:200; BA0056, Boster) antibodies overnight, which was followed by incubation with secondary antibody for 1 h. The nuclear DNA was labeled with DAPI.

    Techniques: Cell Culture, Light Microscopy, Expressing, MTT Assay

    Graphene oxide reduces the expression of stem cell markers and promotes the differentiation of GSCs. a Quantification of the mRNA levels of stem cell markers SOX2 and CD133 in GSCs with or without treatment with GO. b The intracellular expression of the differentiation marker GFAP after treatment with 50 μg/ml GO was examined using immunofluorescence staining. Scale bar = 100 μm. c The expression level of the stem cell marker CD133 in cells treated with different concentrations of GO was detected by immunofluorescence staining. Scale bar = 50 μm. d , e Representative immunoblots and relative quantification of OCT4, SOX2, TUJ1 and GFAP in GSCs after treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Journal: Journal of Translational Medicine

    Article Title: Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms

    doi: 10.1186/s12967-020-02359-z

    Figure Lengend Snippet: Graphene oxide reduces the expression of stem cell markers and promotes the differentiation of GSCs. a Quantification of the mRNA levels of stem cell markers SOX2 and CD133 in GSCs with or without treatment with GO. b The intracellular expression of the differentiation marker GFAP after treatment with 50 μg/ml GO was examined using immunofluorescence staining. Scale bar = 100 μm. c The expression level of the stem cell marker CD133 in cells treated with different concentrations of GO was detected by immunofluorescence staining. Scale bar = 50 μm. d , e Representative immunoblots and relative quantification of OCT4, SOX2, TUJ1 and GFAP in GSCs after treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Article Snippet: We performed incubation with anti-CD133 (1:200; PB0168, Boster) and anti-GFAP (1:200; BA0056, Boster) antibodies overnight, which was followed by incubation with secondary antibody for 1 h. The nuclear DNA was labeled with DAPI.

    Techniques: Expressing, Marker, Immunofluorescence, Staining, Western Blot