rabbit anti extracellular p2x7r igg (Alomone Labs)


Structured Review

Rabbit Anti Extracellular P2x7r Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti extracellular p2x7r igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus"
Article Title: Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus
Journal: Journal of Neuroinflammation
doi: 10.1186/s12974-020-02058-y

Figure Legend Snippet: The effects of L-NAME and/or PDI knockdown on the amounts of SNO-thiol and total thiol-PDI or -P2X7R, and PDI-P2X7R bindings following SE. As compared to vehicle (V), L-NAME (L) reduces the amount of SNO-thiol-PDI and PDI-P2X7R bindings without affecting PDI expression and the amount of total thiol-PDI under physiological condition. Thus, S -nitrosylation ratio of PDI is decreased. L-NAME abrogates SE-induced alterations in PDI-P2X7R bindings and S -nitrosylation ratio of PDI, except PDI expression. As compared to control siRNA (C), PDI siRNA (P) abolishes the changes in PDI-P2X7R bindings and S -nitrosylation ratio of P2X7R following SE, except its expression. a Representative Western blot for S -nitrosylation and thiolization on PDI and its expression. b Quantification of analyses of S -nitrosylation and thiolization on PDI and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM ( * # p
Techniques Used: Expressing, Western Blot
![... of the role of PDI-mediated S -nitrosylation of P2X7R in its trafficking to the cell membrane. SE ... Scheme of the role of PDI-mediated S -nitrosylation of P2X7R in its trafficking to the cell membrane. SE elevates NO synthesis, which S -nitrosylates PDI. SNO-PDI transfers NO to the reduced (immature) P2X7R. In turn, SNO-P2X7R is oxidized by denitrosylases (presumably Trx-1 [ 8 ]), which facilitates its trafficking to the cell surface](https://storage.googleapis.com/bioz_article_images/PMC7788848/12974_2020_2058_Fig8_HTML.jpg)
Figure Legend Snippet: Scheme of the role of PDI-mediated S -nitrosylation of P2X7R in its trafficking to the cell membrane. SE elevates NO synthesis, which S -nitrosylates PDI. SNO-PDI transfers NO to the reduced (immature) P2X7R. In turn, SNO-P2X7R is oxidized by denitrosylases (presumably Trx-1 [ 8 ]), which facilitates its trafficking to the cell surface
Techniques Used:

Figure Legend Snippet: The effects of L-NAME and PDI knockdown on the surface expression of P2X7R. As compared to control animals, SE increases the membrane P2X7R expressions, which are abrogated by both L-NAME and PDI siRNA. a Representative Western blot for surface expression of P2X7R following SE. b Quantification of analyses of P2X7R expression in membrane fractions. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM ( * # p
Techniques Used: Expressing, Western Blot

Figure Legend Snippet: The effects of L-NAME on the amounts of SNO-thiol- and total thiol-P2X7R following SE. As compared to vehicle (V), L-NAME (L) reduces the amount of SNO-thiol-P2X7R and increased that of total thiol-P2X7R without altering P2X7R expression under physiological condition. Thus, S -nitrosylation ratio of P2X7R is decreased. SE upregulates P2X7R expression and S -nitrosylation ratio of P2X7R due to the reduced the amount of total thiol without the altered that of SNO-thiol-P2X7R. L-NAME inhibits these alterations induced by SE, except P2X7R expression. a Representative Western blot for S -nitrosylation and thiolization on P2X7R and its expression. b-e Quantification of analyses of S -nitrosylation and thiolization on P2X7R and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM ( * # p
Techniques Used: Expressing, Western Blot
2) Product Images from "Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus"
Article Title: Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus
Journal: Journal of Neuroinflammation
doi: 10.1186/s12974-020-02058-y

Figure Legend Snippet: The effects of L-NAME and/or PDI knockdown on the amounts of SNO-thiol and total thiol-PDI or -P2X7R, and PDI-P2X7R bindings following SE. As compared to vehicle (V), L-NAME (L) reduces the amount of SNO-thiol-PDI and PDI-P2X7R bindings without affecting PDI expression and the amount of total thiol-PDI under physiological condition. Thus, S -nitrosylation ratio of PDI is decreased. L-NAME abrogates SE-induced alterations in PDI-P2X7R bindings and S -nitrosylation ratio of PDI, except PDI expression. As compared to control siRNA (C), PDI siRNA (P) abolishes the changes in PDI-P2X7R bindings and S -nitrosylation ratio of P2X7R following SE, except its expression. a Representative Western blot for S -nitrosylation and thiolization on PDI and its expression. b Quantification of analyses of S -nitrosylation and thiolization on PDI and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM ( * # p
Techniques Used: Expressing, Western Blot
![... of the role of PDI-mediated S -nitrosylation of P2X7R in its trafficking to the cell membrane. SE ... Scheme of the role of PDI-mediated S -nitrosylation of P2X7R in its trafficking to the cell membrane. SE elevates NO synthesis, which S -nitrosylates PDI. SNO-PDI transfers NO to the reduced (immature) P2X7R. In turn, SNO-P2X7R is oxidized by denitrosylases (presumably Trx-1 [ 8 ]), which facilitates its trafficking to the cell surface](https://storage.googleapis.com/bioz_article_images/PMC7788848/12974_2020_2058_Fig8_HTML.jpg)
Figure Legend Snippet: Scheme of the role of PDI-mediated S -nitrosylation of P2X7R in its trafficking to the cell membrane. SE elevates NO synthesis, which S -nitrosylates PDI. SNO-PDI transfers NO to the reduced (immature) P2X7R. In turn, SNO-P2X7R is oxidized by denitrosylases (presumably Trx-1 [ 8 ]), which facilitates its trafficking to the cell surface
Techniques Used:

Figure Legend Snippet: The effects of L-NAME and PDI knockdown on the surface expression of P2X7R. As compared to control animals, SE increases the membrane P2X7R expressions, which are abrogated by both L-NAME and PDI siRNA. a Representative Western blot for surface expression of P2X7R following SE. b Quantification of analyses of P2X7R expression in membrane fractions. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM ( * # p
Techniques Used: Expressing, Western Blot

Figure Legend Snippet: The effects of L-NAME on the amounts of SNO-thiol- and total thiol-P2X7R following SE. As compared to vehicle (V), L-NAME (L) reduces the amount of SNO-thiol-P2X7R and increased that of total thiol-P2X7R without altering P2X7R expression under physiological condition. Thus, S -nitrosylation ratio of P2X7R is decreased. SE upregulates P2X7R expression and S -nitrosylation ratio of P2X7R due to the reduced the amount of total thiol without the altered that of SNO-thiol-P2X7R. L-NAME inhibits these alterations induced by SE, except P2X7R expression. a Representative Western blot for S -nitrosylation and thiolization on P2X7R and its expression. b-e Quantification of analyses of S -nitrosylation and thiolization on P2X7R and its expression. Open circles indicate each individual value. Horizontal bars indicate the mean value. Error bars indicate SEM ( * # p
Techniques Used: Expressing, Western Blot