rabbit monoclonal anti cyclin d1  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti cyclin d1
    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and <t>cyclin</t> <t>D1,</t> with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Rabbit Monoclonal Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Proteomic insights into the regulatory function of ARID1A in colon cancer cells"

    Article Title: Proteomic insights into the regulatory function of ARID1A in colon cancer cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2024.14525

    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Figure Legend Snippet: Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.

    Techniques Used: Generated, Western Blot, Control, Standard Deviation, Over Expression, Molecular Weight

    rabbit anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti cyclin d1
    Rabbit Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    cyclin d1 (e3p5s) xp® rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc cyclin d1 (e3p5s) xp® rabbit mab
    Cyclin D1 (E3p5s) Xp® Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1 (e3p5s) xp® rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 (e3p5s) xp® rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    rabbit anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti cyclin d1
    Rabbit Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    rabbit anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti cyclin d1
    (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and <t>Cyclin</t> <t>D1</t> normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.
    Rabbit Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration"

    Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

    Journal: Toxicology

    doi: 10.1016/j.tox.2024.153804

    (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and Cyclin D1 normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.
    Figure Legend Snippet: (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and Cyclin D1 normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry

    rabbit anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti cyclin d1
    (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and <t>Cyclin</t> <t>D1</t> normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.
    Rabbit Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration"

    Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

    Journal: Toxicology

    doi: 10.1016/j.tox.2024.153804

    (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and Cyclin D1 normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.
    Figure Legend Snippet: (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and Cyclin D1 normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry

    rabbit monoclonal 92g2 anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal 92g2 anti cyclin d1
    Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, <t>cyclin</t> <t>D1,</t> and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.
    Rabbit Monoclonal 92g2 Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal 92g2 anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal 92g2 anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "TERC promotes non-small cell lung cancer progression by facilitating the nuclear localization of TERT"

    Article Title: TERC promotes non-small cell lung cancer progression by facilitating the nuclear localization of TERT

    Journal: iScience

    doi: 10.1016/j.isci.2024.109869

    Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, cyclin D1, and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.
    Figure Legend Snippet: Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, cyclin D1, and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.

    Techniques Used: Binding Assay, Expressing, Quantitative RT-PCR, Stable Transfection, Western Blot


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Modification, Immunoprecipitation, Transfection, Membrane, DNA Purification, SYBR Green Assay, Isolation, Staining, Methylation, Software

    anti cyclin d1 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti cyclin d1 rabbit mab
    Anti Cyclin D1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cyclin d1 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

    Images

    rabbit monoclonal anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti cyclin d1
    (A–C) PH was performed on littermates, and the liver was harvested 2–14 days later. Liver cyclins A2 and <t>D1</t> mRNA levels were measured by qPCR (normalized to 36B4 expression). Liver extracts were prepared 2 days after PH and immunoblotted with antibodies to <t>cyclin</t> A2, cyclin D1, cyclin E1, and GAPDH. Cyclin levels were normalized to GAPDH levels. (A) Day 0: Snai1/2 f/f : n = 6, Snai1/2 Δhep : n = 7; day 2: Snai1/2 f/f : n = 8, Snai1/2 Δhep : n = 6; days 7 and 14: n = 8 per group. (B) n = 4 mice per group. (C) mRNA levels: n = 5 mice per group; protein levels: n = 4 mice per group. (D) Snai1/2 f/f and Snai1/2 Δhep males (8 weeks) were treated with CCl4 for 48 h (1 μL/g body weight). Liver extracts were immunoblotted with antibodies to cyclin A2, cyclin D1, cyclin E1, and GAPDH. Cyclin levels were normalized to GAPDH levels (n = 4 mice per group). (E) Snai1/2 f/f and Snai1/2 Δhep males (8 weeks) were treated with CCl4 for 24 days (0.6 μL/g body weight, twice a week). Liver cyclin A2, cyclin D1, and cyclin E1 mRNA (normalized to 36B4, Snai1/2 f/f n = 9, Snai1/2 Δhep : n = 7) and protein (normalized to GAPDH, Snai1/2 f/f : n = 7, Snai1/2 Δhep : n = 5) levels were measured by qPCR and immunoblotting, respectively. (F and G) Primary hepatocytes were transduced with the indicated adenoviral vectors for 48 h. (F) Cyclin A2, cyclin D1, and cyclin E1 mRNA levels (normalized to 36B4 levels, n = 3 repeats per group). (G) Cell extracts were immunoblotted with the indicated antibodies. (H) C57BL/6J males (8 weeks) were transduced with AAV-CAG-Snai2 or AAV-CAG-GFP vectors for 2 weeks and then treated with CCl4 for 72 h (1 μL/g body weight). Liver cyclin A2, cyclin D1, and cyclin E1 mRNA (normalized to 36B4, n = 6 mice per group) and protein (normalized to GAPDH, n = 3 mice per group) levels were measured by qPCR and immunoblotting, respectively. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, two-tailed unpaired Student’s t test.
    Rabbit Monoclonal Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice"

    Article Title: Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113875

    (A–C) PH was performed on littermates, and the liver was harvested 2–14 days later. Liver cyclins A2 and D1 mRNA levels were measured by qPCR (normalized to 36B4 expression). Liver extracts were prepared 2 days after PH and immunoblotted with antibodies to cyclin A2, cyclin D1, cyclin E1, and GAPDH. Cyclin levels were normalized to GAPDH levels. (A) Day 0: Snai1/2 f/f : n = 6, Snai1/2 Δhep : n = 7; day 2: Snai1/2 f/f : n = 8, Snai1/2 Δhep : n = 6; days 7 and 14: n = 8 per group. (B) n = 4 mice per group. (C) mRNA levels: n = 5 mice per group; protein levels: n = 4 mice per group. (D) Snai1/2 f/f and Snai1/2 Δhep males (8 weeks) were treated with CCl4 for 48 h (1 μL/g body weight). Liver extracts were immunoblotted with antibodies to cyclin A2, cyclin D1, cyclin E1, and GAPDH. Cyclin levels were normalized to GAPDH levels (n = 4 mice per group). (E) Snai1/2 f/f and Snai1/2 Δhep males (8 weeks) were treated with CCl4 for 24 days (0.6 μL/g body weight, twice a week). Liver cyclin A2, cyclin D1, and cyclin E1 mRNA (normalized to 36B4, Snai1/2 f/f n = 9, Snai1/2 Δhep : n = 7) and protein (normalized to GAPDH, Snai1/2 f/f : n = 7, Snai1/2 Δhep : n = 5) levels were measured by qPCR and immunoblotting, respectively. (F and G) Primary hepatocytes were transduced with the indicated adenoviral vectors for 48 h. (F) Cyclin A2, cyclin D1, and cyclin E1 mRNA levels (normalized to 36B4 levels, n = 3 repeats per group). (G) Cell extracts were immunoblotted with the indicated antibodies. (H) C57BL/6J males (8 weeks) were transduced with AAV-CAG-Snai2 or AAV-CAG-GFP vectors for 2 weeks and then treated with CCl4 for 72 h (1 μL/g body weight). Liver cyclin A2, cyclin D1, and cyclin E1 mRNA (normalized to 36B4, n = 6 mice per group) and protein (normalized to GAPDH, n = 3 mice per group) levels were measured by qPCR and immunoblotting, respectively. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, two-tailed unpaired Student’s t test.
    Figure Legend Snippet: (A–C) PH was performed on littermates, and the liver was harvested 2–14 days later. Liver cyclins A2 and D1 mRNA levels were measured by qPCR (normalized to 36B4 expression). Liver extracts were prepared 2 days after PH and immunoblotted with antibodies to cyclin A2, cyclin D1, cyclin E1, and GAPDH. Cyclin levels were normalized to GAPDH levels. (A) Day 0: Snai1/2 f/f : n = 6, Snai1/2 Δhep : n = 7; day 2: Snai1/2 f/f : n = 8, Snai1/2 Δhep : n = 6; days 7 and 14: n = 8 per group. (B) n = 4 mice per group. (C) mRNA levels: n = 5 mice per group; protein levels: n = 4 mice per group. (D) Snai1/2 f/f and Snai1/2 Δhep males (8 weeks) were treated with CCl4 for 48 h (1 μL/g body weight). Liver extracts were immunoblotted with antibodies to cyclin A2, cyclin D1, cyclin E1, and GAPDH. Cyclin levels were normalized to GAPDH levels (n = 4 mice per group). (E) Snai1/2 f/f and Snai1/2 Δhep males (8 weeks) were treated with CCl4 for 24 days (0.6 μL/g body weight, twice a week). Liver cyclin A2, cyclin D1, and cyclin E1 mRNA (normalized to 36B4, Snai1/2 f/f n = 9, Snai1/2 Δhep : n = 7) and protein (normalized to GAPDH, Snai1/2 f/f : n = 7, Snai1/2 Δhep : n = 5) levels were measured by qPCR and immunoblotting, respectively. (F and G) Primary hepatocytes were transduced with the indicated adenoviral vectors for 48 h. (F) Cyclin A2, cyclin D1, and cyclin E1 mRNA levels (normalized to 36B4 levels, n = 3 repeats per group). (G) Cell extracts were immunoblotted with the indicated antibodies. (H) C57BL/6J males (8 weeks) were transduced with AAV-CAG-Snai2 or AAV-CAG-GFP vectors for 2 weeks and then treated with CCl4 for 72 h (1 μL/g body weight). Liver cyclin A2, cyclin D1, and cyclin E1 mRNA (normalized to 36B4, n = 6 mice per group) and protein (normalized to GAPDH, n = 3 mice per group) levels were measured by qPCR and immunoblotting, respectively. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, two-tailed unpaired Student’s t test.

    Techniques Used: Expressing, Western Blot, Transduction, Two Tailed Test

    (A and B) Primary hepatocytes were transduced with Snai1 (n = 4), Snai2 (n = 4), or β-galactosidase (β-gal) (n = 3) adenoviral vectors for 2 days. (A) ChIP-qPCR images. (B) Occupancies of Snai1 and Snai2 on the Ccna2 and Ccnd1 promoters. (C and D) Wild-type male mice were treated with PH or CCl4 for 48 h. Occupancies of Snai1 and Snai2 on the Ccna2 and Ccnd1 promoters in the liver were measured using ChIP-qPCR. PH: n = 6 per group, control (Con): n = 6, CCl4: n = 5. (E) H3K27ac levels in Ccna2 and Ccnd1 promoters (n = 4 per group). (F) C57BL/6J males (8 weeks) were transduced with AAV8-CAG-Snai2 (n = 6) or AAV8-CAG-GFP (n = 6) vectors for 3 weeks. Snai2 occupancies and H3K27ac levels on Ccna2 and Ccnd1 promoters were measured in the liver by ChIP-qPCR. (G) Livers were harvested from Snai1/2 f/f (n = 4) and Snai1/2 Δhep (n = 4) males (8 weeks old) and subjected to ChIP to measure H3K27ac levels in Ccna2 and Ccnd1 promoters. (H) Snai2 was coexpressed with p300 in HEK293T cells, immunoprecipitated with anti-Snai2 (or anti-300) antibody, and immunoblotted with anti-p300 (or anti-Snai2) antibody. (I) CBP, p300, or β-gal plasmids were cotransfected with or without Snai2 plasmids in HEK293T cells (with pGL3-Ccna2 cotransfection). CCNA2 promoter H3K27ac levels were measured by ChIP-qPCR (n = 4 per group). (J) Ccna2 luciferase reporter plasmids (pGL3-Ccna2) or pGL3-basic plasmids (Con) were cotransfected with GFP, Snai1, Snai2, or ΔN30 into Huh 7 cells. Luciferase activity was measured 48 h later (n = 3 repeats per group). (K) Ccna2 luciferase reporter plasmids were cotransfected into Huh 7 cells with GFP, CBP, or p300 plasmids in the presence of GFP or Snai2 plasmids. Luciferase activity was measured 48 h later (n = 3 per group). (L) Snai1 and Snai2 recruit CBP and p300 that catalyze H3K27ac (active epigenetic mark) in cyclin A2 and D1 promoters, thereby enhancing hepatocyte proliferation and liver regeneration. Snai1/2-induced hepatocyte renewal suppresses liver fibrosis. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, two-tailed unpaired Student’s t test (B–G) and one-way ANOVA (I–K).
    Figure Legend Snippet: (A and B) Primary hepatocytes were transduced with Snai1 (n = 4), Snai2 (n = 4), or β-galactosidase (β-gal) (n = 3) adenoviral vectors for 2 days. (A) ChIP-qPCR images. (B) Occupancies of Snai1 and Snai2 on the Ccna2 and Ccnd1 promoters. (C and D) Wild-type male mice were treated with PH or CCl4 for 48 h. Occupancies of Snai1 and Snai2 on the Ccna2 and Ccnd1 promoters in the liver were measured using ChIP-qPCR. PH: n = 6 per group, control (Con): n = 6, CCl4: n = 5. (E) H3K27ac levels in Ccna2 and Ccnd1 promoters (n = 4 per group). (F) C57BL/6J males (8 weeks) were transduced with AAV8-CAG-Snai2 (n = 6) or AAV8-CAG-GFP (n = 6) vectors for 3 weeks. Snai2 occupancies and H3K27ac levels on Ccna2 and Ccnd1 promoters were measured in the liver by ChIP-qPCR. (G) Livers were harvested from Snai1/2 f/f (n = 4) and Snai1/2 Δhep (n = 4) males (8 weeks old) and subjected to ChIP to measure H3K27ac levels in Ccna2 and Ccnd1 promoters. (H) Snai2 was coexpressed with p300 in HEK293T cells, immunoprecipitated with anti-Snai2 (or anti-300) antibody, and immunoblotted with anti-p300 (or anti-Snai2) antibody. (I) CBP, p300, or β-gal plasmids were cotransfected with or without Snai2 plasmids in HEK293T cells (with pGL3-Ccna2 cotransfection). CCNA2 promoter H3K27ac levels were measured by ChIP-qPCR (n = 4 per group). (J) Ccna2 luciferase reporter plasmids (pGL3-Ccna2) or pGL3-basic plasmids (Con) were cotransfected with GFP, Snai1, Snai2, or ΔN30 into Huh 7 cells. Luciferase activity was measured 48 h later (n = 3 repeats per group). (K) Ccna2 luciferase reporter plasmids were cotransfected into Huh 7 cells with GFP, CBP, or p300 plasmids in the presence of GFP or Snai2 plasmids. Luciferase activity was measured 48 h later (n = 3 per group). (L) Snai1 and Snai2 recruit CBP and p300 that catalyze H3K27ac (active epigenetic mark) in cyclin A2 and D1 promoters, thereby enhancing hepatocyte proliferation and liver regeneration. Snai1/2-induced hepatocyte renewal suppresses liver fibrosis. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, two-tailed unpaired Student’s t test (B–G) and one-way ANOVA (I–K).

    Techniques Used: Transduction, Immunoprecipitation, Cotransfection, Luciferase, Activity Assay, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Virus, In Situ, Real-time Polymerase Chain Reaction, Recombinant, Luciferase, Software

    rabbit anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti cyclin d1
    Rabbit Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Cell Signaling Technology Inc rabbit monoclonal anti cyclin d1
    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and <t>cyclin</t> <t>D1,</t> with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Rabbit Monoclonal Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit anti cyclin d1
    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and <t>cyclin</t> <t>D1,</t> with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Rabbit Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc cyclin d1 (e3p5s) xp® rabbit mab
    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and <t>cyclin</t> <t>D1,</t> with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Cyclin D1 (E3p5s) Xp® Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1 (e3p5s) xp® rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 (e3p5s) xp® rabbit mab - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit monoclonal 92g2 anti cyclin d1
    Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, <t>cyclin</t> <t>D1,</t> and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.
    Rabbit Monoclonal 92g2 Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal 92g2 anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal 92g2 anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti cyclin d1 rabbit mab
    Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, <t>cyclin</t> <t>D1,</t> and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.
    Anti Cyclin D1 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cyclin d1 rabbit mab - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.

    Journal: Oncology Letters

    Article Title: Proteomic insights into the regulatory function of ARID1A in colon cancer cells

    doi: 10.3892/ol.2024.14525

    Figure Lengend Snippet: Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.

    Article Snippet: The membrane was incubated with 5% skimmed milk in PBS at 25°C for 1 h. Subsequently, it was incubated with rabbit polyclonal anti-ARID1A (1:1,000; cat. no. HPA005456; Sigma-Aldrich; Merck KGaA), rabbit monoclonal anti-c-Myc (1:1,000; cat. no. 5605; Cell Signaling Technology, Inc.), rabbit monoclonal anti-T cell factor (TCF)1/7 (1:1,000; cat. no. 2203; Cell Signaling Technology, Inc.), rabbit monoclonal anti-cyclin D1 (1:1,000; cat. no. 2978; Cell Signaling Technology, Inc.), rabbit polyclonal anti-zinc and ring finger 3 (ZNRF3; 1:1,000; cat. no. DF14289; Affinity Biosciences), or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam) antibodies at 4°C overnight.

    Techniques: Generated, Western Blot, Control, Standard Deviation, Over Expression, Molecular Weight

    Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, cyclin D1, and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.

    Journal: iScience

    Article Title: TERC promotes non-small cell lung cancer progression by facilitating the nuclear localization of TERT

    doi: 10.1016/j.isci.2024.109869

    Figure Lengend Snippet: Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, cyclin D1, and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.

    Article Snippet: Rabbit monoclonal (92G2) anti-Cyclin D1 , Cell Signaling Technology , Cat#2978; RRID: AB_2259616.

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Stable Transfection, Western Blot

    Journal: iScience

    Article Title: TERC promotes non-small cell lung cancer progression by facilitating the nuclear localization of TERT

    doi: 10.1016/j.isci.2024.109869

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal (92G2) anti-Cyclin D1 , Cell Signaling Technology , Cat#2978; RRID: AB_2259616.

    Techniques: Virus, Recombinant, Modification, Immunoprecipitation, Transfection, Membrane, DNA Purification, SYBR Green Assay, Isolation, Staining, Methylation, Software