rabbit anti claudin 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti claudin 1
    Rabbit Anti Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti claudin 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti claudin 1
    Representative western blotting illustrated the decrease in the pFAK/tFAK ratio and the inhibition of EMT markers expression in KKU-213 cells transfected with LAMA3 siRNA. Treated cells were reseeded for 24 h before protein extraction and GAPDH was used as the internal control. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. Full-length blots are presented in Supplementary Fig. . ( a ) Expression of FAK and its phosphorylation at Y397. ( b ) Expression of EMT-markers, including vimentin and Slug, and the epithelial markers, E-cadherin and <t>claudin-1.</t> Band intensity was showed as mean ± SEM in percent relative to those of the siNeg from at least four independent experiments. Graphs were created using Graphpad Prism. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Rabbit Anti Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Upregulated LAMA3 modulates proliferation, adhesion, migration and epithelial‑to‑mesenchymal transition of cholangiocarcinoma cells"

    Article Title: Upregulated LAMA3 modulates proliferation, adhesion, migration and epithelial‑to‑mesenchymal transition of cholangiocarcinoma cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-48798-8

    Representative western blotting illustrated the decrease in the pFAK/tFAK ratio and the inhibition of EMT markers expression in KKU-213 cells transfected with LAMA3 siRNA. Treated cells were reseeded for 24 h before protein extraction and GAPDH was used as the internal control. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. Full-length blots are presented in Supplementary Fig. . ( a ) Expression of FAK and its phosphorylation at Y397. ( b ) Expression of EMT-markers, including vimentin and Slug, and the epithelial markers, E-cadherin and claudin-1. Band intensity was showed as mean ± SEM in percent relative to those of the siNeg from at least four independent experiments. Graphs were created using Graphpad Prism. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: Representative western blotting illustrated the decrease in the pFAK/tFAK ratio and the inhibition of EMT markers expression in KKU-213 cells transfected with LAMA3 siRNA. Treated cells were reseeded for 24 h before protein extraction and GAPDH was used as the internal control. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. Full-length blots are presented in Supplementary Fig. . ( a ) Expression of FAK and its phosphorylation at Y397. ( b ) Expression of EMT-markers, including vimentin and Slug, and the epithelial markers, E-cadherin and claudin-1. Band intensity was showed as mean ± SEM in percent relative to those of the siNeg from at least four independent experiments. Graphs were created using Graphpad Prism. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Western Blot, Inhibition, Expressing, Transfection, Protein Extraction

    anti rabbit claudin 1 antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti rabbit claudin 1 antibody
    Anti Rabbit Claudin 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti rabbit claudin 1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti rabbit claudin 1 antibody
    Effects of CM powder on intestinal mucosal barrier in DSS-induced ulcerative colitis mice. ( A ) Representative images of H&E staining (magnification of 100× and 200×) and its histopathological evaluation ( n = 8). ( B – E ) Representative images (magnification of 200×) of immunofluorescence staining of MUC2, ZO-1, claudin 1, and occludin, and its mean fluorescent intensity ( n = 3). All images were taken at the same scale. ##, p < 0.01 versus control group; *, p < 0.05 versus model group; **, p < 0.01 versus model group; n.s., no significant difference ( p > 0.05).
    Anti Rabbit Claudin 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit claudin 1 antibody/product/Cell Signaling Technology Inc
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    Images

    1) Product Images from "Effect of Cordyceps militaris Powder Prophylactic Supplementation on Intestinal Mucosal Barrier Impairment and Microbiota-Metabolites Axis in DSS-Injured Mice"

    Article Title: Effect of Cordyceps militaris Powder Prophylactic Supplementation on Intestinal Mucosal Barrier Impairment and Microbiota-Metabolites Axis in DSS-Injured Mice

    Journal: Nutrients

    doi: 10.3390/nu15204378

    Effects of CM powder on intestinal mucosal barrier in DSS-induced ulcerative colitis mice. ( A ) Representative images of H&E staining (magnification of 100× and 200×) and its histopathological evaluation ( n = 8). ( B – E ) Representative images (magnification of 200×) of immunofluorescence staining of MUC2, ZO-1, claudin 1, and occludin, and its mean fluorescent intensity ( n = 3). All images were taken at the same scale. ##, p < 0.01 versus control group; *, p < 0.05 versus model group; **, p < 0.01 versus model group; n.s., no significant difference ( p > 0.05).
    Figure Legend Snippet: Effects of CM powder on intestinal mucosal barrier in DSS-induced ulcerative colitis mice. ( A ) Representative images of H&E staining (magnification of 100× and 200×) and its histopathological evaluation ( n = 8). ( B – E ) Representative images (magnification of 200×) of immunofluorescence staining of MUC2, ZO-1, claudin 1, and occludin, and its mean fluorescent intensity ( n = 3). All images were taken at the same scale. ##, p < 0.01 versus control group; *, p < 0.05 versus model group; **, p < 0.01 versus model group; n.s., no significant difference ( p > 0.05).

    Techniques Used: Staining, Immunofluorescence

    rabbit anti human claudin 1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti human claudin 1
    Rabbit Anti Human Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human claudin 1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti human claudin 1
    Rabbit Anti Human Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti nf κb  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti nf κb
    Rabbit Anti Nf κb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    claudin 1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc claudin 1
    Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    claudin 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc claudin 1
    PKCδ and NDRG1 were co-located in CRC tissues. (A) The expression of PKCδ and NDRG1 in CRC tumor tissues, detected by immunofluorescence histochemistry. (B) The expression of PKCδ and NDRG1 in colorectal normal tissues, detected by immunofluorescence histochemistry. (C) The typical immunofluorescence images of PKCδ and NDRG1 in CRC tumor tissues for co-localization analysis. (D) Enlarged view of the image in the red box, which indicated the co-localization of PKCδ and NDRG1 in CRC, with the pixel acquisition area by the yellow line, detected by immunofluorescence histochemistry. (E) Pixel loci analysis of the expression of PKCδ and NDRG1 in colorectal cancer. (F) Analysis of the co-localization relationship of PKCδ and NDRG1 in CRC by software Fiji Image J, with the Pearson ’ s R-value 0.80, which indicated that PKCδ and NDRG1 were co-located strongly in CRC. (G) Co-immunoprecipitation was used to detect the relationship of PKCδ and NDRG1. (H) NDRG1 inhibited the migration of CRC cells by cell migration assay. (I) Columns were used to quantify the migrated and invasive cells in (H) . (I) Knocking down NDRG1 downregulated the epithelial marker <t>Claudin-1</t> and upregulated the mesenchymal marker N-cadherin and Vimentin expression, while overexpression of NDRG1 showed the opposite trend in RKO cells, determined by Western blots. (K) Columns were used to quantify the protein expression in (J) relative to GAPDH. Scale bar. 20µm.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/claudin 1/product/Cell Signaling Technology Inc
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    1) Product Images from "PKCδ promotes the invasion and migration of colorectal cancer through c-myc/NDRG1 pathway"

    Article Title: PKCδ promotes the invasion and migration of colorectal cancer through c-myc/NDRG1 pathway

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2023.1026561

    PKCδ and NDRG1 were co-located in CRC tissues. (A) The expression of PKCδ and NDRG1 in CRC tumor tissues, detected by immunofluorescence histochemistry. (B) The expression of PKCδ and NDRG1 in colorectal normal tissues, detected by immunofluorescence histochemistry. (C) The typical immunofluorescence images of PKCδ and NDRG1 in CRC tumor tissues for co-localization analysis. (D) Enlarged view of the image in the red box, which indicated the co-localization of PKCδ and NDRG1 in CRC, with the pixel acquisition area by the yellow line, detected by immunofluorescence histochemistry. (E) Pixel loci analysis of the expression of PKCδ and NDRG1 in colorectal cancer. (F) Analysis of the co-localization relationship of PKCδ and NDRG1 in CRC by software Fiji Image J, with the Pearson ’ s R-value 0.80, which indicated that PKCδ and NDRG1 were co-located strongly in CRC. (G) Co-immunoprecipitation was used to detect the relationship of PKCδ and NDRG1. (H) NDRG1 inhibited the migration of CRC cells by cell migration assay. (I) Columns were used to quantify the migrated and invasive cells in (H) . (I) Knocking down NDRG1 downregulated the epithelial marker Claudin-1 and upregulated the mesenchymal marker N-cadherin and Vimentin expression, while overexpression of NDRG1 showed the opposite trend in RKO cells, determined by Western blots. (K) Columns were used to quantify the protein expression in (J) relative to GAPDH. Scale bar. 20µm.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: PKCδ and NDRG1 were co-located in CRC tissues. (A) The expression of PKCδ and NDRG1 in CRC tumor tissues, detected by immunofluorescence histochemistry. (B) The expression of PKCδ and NDRG1 in colorectal normal tissues, detected by immunofluorescence histochemistry. (C) The typical immunofluorescence images of PKCδ and NDRG1 in CRC tumor tissues for co-localization analysis. (D) Enlarged view of the image in the red box, which indicated the co-localization of PKCδ and NDRG1 in CRC, with the pixel acquisition area by the yellow line, detected by immunofluorescence histochemistry. (E) Pixel loci analysis of the expression of PKCδ and NDRG1 in colorectal cancer. (F) Analysis of the co-localization relationship of PKCδ and NDRG1 in CRC by software Fiji Image J, with the Pearson ’ s R-value 0.80, which indicated that PKCδ and NDRG1 were co-located strongly in CRC. (G) Co-immunoprecipitation was used to detect the relationship of PKCδ and NDRG1. (H) NDRG1 inhibited the migration of CRC cells by cell migration assay. (I) Columns were used to quantify the migrated and invasive cells in (H) . (I) Knocking down NDRG1 downregulated the epithelial marker Claudin-1 and upregulated the mesenchymal marker N-cadherin and Vimentin expression, while overexpression of NDRG1 showed the opposite trend in RKO cells, determined by Western blots. (K) Columns were used to quantify the protein expression in (J) relative to GAPDH. Scale bar. 20µm.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Expressing, Immunofluorescence, Software, Immunoprecipitation, Migration, Cell Migration Assay, Marker, Over Expression, Western Blot

    anti claudin 1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti claudin 1
    A Western blot analysis of EMT marker expression in DDX17-overexpressing and mock SW480 cells. B Western blot analysis of EMT marker expression in DDX17-deficient and control SW620 cells. C Representative immunohistochemical staining images of DDX17, E-cadherin, and <t>Claudin-1</t> levels in CRC tissues. D Correlation analysis between the protein expressions of DDX17 and E-cadherin in CRC tissues. E Correlation analysis between the protein expressions of DDX17 and claudin-1 in CRC tissues.
    Anti Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti claudin 1/product/Cell Signaling Technology Inc
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    anti claudin 1 - by Bioz Stars, 2024-04
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    Images

    1) Product Images from "DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer"

    Article Title: DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-022-05508-y

    A Western blot analysis of EMT marker expression in DDX17-overexpressing and mock SW480 cells. B Western blot analysis of EMT marker expression in DDX17-deficient and control SW620 cells. C Representative immunohistochemical staining images of DDX17, E-cadherin, and Claudin-1 levels in CRC tissues. D Correlation analysis between the protein expressions of DDX17 and E-cadherin in CRC tissues. E Correlation analysis between the protein expressions of DDX17 and claudin-1 in CRC tissues.
    Figure Legend Snippet: A Western blot analysis of EMT marker expression in DDX17-overexpressing and mock SW480 cells. B Western blot analysis of EMT marker expression in DDX17-deficient and control SW620 cells. C Representative immunohistochemical staining images of DDX17, E-cadherin, and Claudin-1 levels in CRC tissues. D Correlation analysis between the protein expressions of DDX17 and E-cadherin in CRC tissues. E Correlation analysis between the protein expressions of DDX17 and claudin-1 in CRC tissues.

    Techniques Used: Western Blot, Marker, Expressing, Immunohistochemical staining, Staining

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    Cell Signaling Technology Inc rabbit anti claudin 1
    Rabbit Anti Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti rabbit claudin 1 antibody
    Anti Rabbit Claudin 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit claudin 1 antibody/product/Cell Signaling Technology Inc
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    Rabbit Anti Human Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc claudin 1
    Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti claudin 1
    A Western blot analysis of EMT marker expression in DDX17-overexpressing and mock SW480 cells. B Western blot analysis of EMT marker expression in DDX17-deficient and control SW620 cells. C Representative immunohistochemical staining images of DDX17, E-cadherin, and <t>Claudin-1</t> levels in CRC tissues. D Correlation analysis between the protein expressions of DDX17 and E-cadherin in CRC tissues. E Correlation analysis between the protein expressions of DDX17 and claudin-1 in CRC tissues.
    Anti Claudin 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A Western blot analysis of EMT marker expression in DDX17-overexpressing and mock SW480 cells. B Western blot analysis of EMT marker expression in DDX17-deficient and control SW620 cells. C Representative immunohistochemical staining images of DDX17, E-cadherin, and Claudin-1 levels in CRC tissues. D Correlation analysis between the protein expressions of DDX17 and E-cadherin in CRC tissues. E Correlation analysis between the protein expressions of DDX17 and claudin-1 in CRC tissues.

    Journal: Cell Death & Disease

    Article Title: DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

    doi: 10.1038/s41419-022-05508-y

    Figure Lengend Snippet: A Western blot analysis of EMT marker expression in DDX17-overexpressing and mock SW480 cells. B Western blot analysis of EMT marker expression in DDX17-deficient and control SW620 cells. C Representative immunohistochemical staining images of DDX17, E-cadherin, and Claudin-1 levels in CRC tissues. D Correlation analysis between the protein expressions of DDX17 and E-cadherin in CRC tissues. E Correlation analysis between the protein expressions of DDX17 and claudin-1 in CRC tissues.

    Article Snippet: Lysates were loaded and separated by SDS–PAGE, transferred to a PVDF membrane (Millipore), and then incubated with the following antibodies: anti-DDX17 (Santa Cruz Biotechnology, sc-398168), anti-DDX5 (Cell Signaling Technology, CST, #9877), anti-CYBRD1 (Abcam, ab66048), anti-E-cadherin (CST, #3195), anti-Claudin-1 (CST, #13255), anti-Vimentin (CST, #5741), anti-N-cadherin (CST, #13116), and anti-β-actin (Sangon, D110001).

    Techniques: Western Blot, Marker, Expressing, Immunohistochemical staining, Staining