cleaved caspase 3  (Boster Bio)


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    Structured Review

    Boster Bio cleaved caspase 3
    The effects of anemonin treatment on the homoeostasis of articular cartilage after DMM surgery. Sections of articular cartilage from the mice with or without anemonin treatment after 8 weeks of DMM surgery and sham operation were analysed by immunohistochemical staining ( n = 5 per group). IHC stainings of ( A – D ) MMP 13, ( E – H ) ADAMTS 5, ( I – L ) collagen X, ( M – P ) Aggrecan and ( Q – T ) cleaved <t>caspase‐3</t> were performed in mice after DMM surgery. ( U – X ) The ratios of immunoreactive positive cells, MMP 13 ( U ), ADAMTS 5 ( V ), collagen X ( W ) and cleaved caspase‐3 ( X ) were analysed. Scale bar: 50 μm ( A – T ). Data are expressed as the mean (symbols) ± 95% confidence intervals (error bar).
    Cleaved Caspase 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3/product/Boster Bio
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL‐1β/ NF‐κB pathway"

    Article Title: Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL‐1β/ NF‐κB pathway

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.13227

    The effects of anemonin treatment on the homoeostasis of articular cartilage after DMM surgery. Sections of articular cartilage from the mice with or without anemonin treatment after 8 weeks of DMM surgery and sham operation were analysed by immunohistochemical staining ( n = 5 per group). IHC stainings of ( A – D ) MMP 13, ( E – H ) ADAMTS 5, ( I – L ) collagen X, ( M – P ) Aggrecan and ( Q – T ) cleaved caspase‐3 were performed in mice after DMM surgery. ( U – X ) The ratios of immunoreactive positive cells, MMP 13 ( U ), ADAMTS 5 ( V ), collagen X ( W ) and cleaved caspase‐3 ( X ) were analysed. Scale bar: 50 μm ( A – T ). Data are expressed as the mean (symbols) ± 95% confidence intervals (error bar).
    Figure Legend Snippet: The effects of anemonin treatment on the homoeostasis of articular cartilage after DMM surgery. Sections of articular cartilage from the mice with or without anemonin treatment after 8 weeks of DMM surgery and sham operation were analysed by immunohistochemical staining ( n = 5 per group). IHC stainings of ( A – D ) MMP 13, ( E – H ) ADAMTS 5, ( I – L ) collagen X, ( M – P ) Aggrecan and ( Q – T ) cleaved caspase‐3 were performed in mice after DMM surgery. ( U – X ) The ratios of immunoreactive positive cells, MMP 13 ( U ), ADAMTS 5 ( V ), collagen X ( W ) and cleaved caspase‐3 ( X ) were analysed. Scale bar: 50 μm ( A – T ). Data are expressed as the mean (symbols) ± 95% confidence intervals (error bar).

    Techniques Used: Mouse Assay, Immunohistochemistry, Staining

    2) Product Images from "Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy"

    Article Title: Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.9584

    Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved caspase-3 and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.
    Figure Legend Snippet: Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved caspase-3 and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.

    Techniques Used: Concentration Assay, Expressing, Western Blot, Flow Cytometry, Cytometry

    3) Product Images from "Morphology and Molecular Mechanisms of Hepatic Injury in Rats under Simulated Weightlessness and the Protective Effects of Resistance Training"

    Article Title: Morphology and Molecular Mechanisms of Hepatic Injury in Rats under Simulated Weightlessness and the Protective Effects of Resistance Training

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0127047

    Immunohistochemical and real-time PCR analysis active of caspase-3. A1-C1: Histological sections of livers in control, TS and TS RT group were stained with active caspase-3 antibody. A2-C2: Histological sections of livers in control, TS and TS RT groups were stained with PBS to replace the caspase-3 antibody as blank control. D: A semi-quantitative analysis of the ratio of active caspase-3 positive staining to the total field. E: Relative mRNA levels of caspase-3 in control, TS and TS RT rat livers (Data are expressed as the mean ± SD. * P
    Figure Legend Snippet: Immunohistochemical and real-time PCR analysis active of caspase-3. A1-C1: Histological sections of livers in control, TS and TS RT group were stained with active caspase-3 antibody. A2-C2: Histological sections of livers in control, TS and TS RT groups were stained with PBS to replace the caspase-3 antibody as blank control. D: A semi-quantitative analysis of the ratio of active caspase-3 positive staining to the total field. E: Relative mRNA levels of caspase-3 in control, TS and TS RT rat livers (Data are expressed as the mean ± SD. * P

    Techniques Used: Immunohistochemistry, Real-time Polymerase Chain Reaction, Staining

    4) Product Images from "Cartilage-specific deletion of Alk5 gene results in a progressive osteoarthritis-like phenotype in mice"

    Article Title: Cartilage-specific deletion of Alk5 gene results in a progressive osteoarthritis-like phenotype in mice

    Journal: Osteoarthritis and cartilage

    doi: 10.1016/j.joca.2017.07.010

    Alk5 deficiency increases articular chondrocyte apoptosis. Knee joint samples were dissected from 2-month-old mice, TUNEL assay and cleaved caspase 3 IHC were performed. A, TUNEL staining showed a significant increase in the number of TUNEL positive cells (arrowheads) in articular cartilage of Alk5 cKO mice compared with that in Cre-negative control mice. Quantitative data were shown in the right panel (The percentage of positive cells in Cre-negative mice were defined as 1, Mann–Whitney U test, n = 4 mice per group). B, IHC analysis showed a significant increase in the number of cleaved caspase 3 positive cells in articular cartilage of Alk5 cKO mice compared with that in Cre-negative control mice. Quantitative data were shown in the right panel (The percentage of positive cells in Cre-negative mice was defined as 1, student’s unpaired t -test, n = 4 mice per group). Scale bar: 100 μm. Data were expressed as the mean ± 95% confidence intervals. In A and B, symbols represent individual mice.
    Figure Legend Snippet: Alk5 deficiency increases articular chondrocyte apoptosis. Knee joint samples were dissected from 2-month-old mice, TUNEL assay and cleaved caspase 3 IHC were performed. A, TUNEL staining showed a significant increase in the number of TUNEL positive cells (arrowheads) in articular cartilage of Alk5 cKO mice compared with that in Cre-negative control mice. Quantitative data were shown in the right panel (The percentage of positive cells in Cre-negative mice were defined as 1, Mann–Whitney U test, n = 4 mice per group). B, IHC analysis showed a significant increase in the number of cleaved caspase 3 positive cells in articular cartilage of Alk5 cKO mice compared with that in Cre-negative control mice. Quantitative data were shown in the right panel (The percentage of positive cells in Cre-negative mice was defined as 1, student’s unpaired t -test, n = 4 mice per group). Scale bar: 100 μm. Data were expressed as the mean ± 95% confidence intervals. In A and B, symbols represent individual mice.

    Techniques Used: Mouse Assay, TUNEL Assay, Immunohistochemistry, Staining, Negative Control, MANN-WHITNEY

    5) Product Images from "Novel lncRNA XLOC_032768 protects against renal tubular epithelial cells apoptosis in renal ischemia–reperfusion injury by regulating FNDC3B/TGF-β1"

    Article Title: Novel lncRNA XLOC_032768 protects against renal tubular epithelial cells apoptosis in renal ischemia–reperfusion injury by regulating FNDC3B/TGF-β1

    Journal: Renal Failure

    doi: 10.1080/0886022X.2020.1818579

    Knockdown of FNDC3B markedly reduced the apoptosis of renal tubular cells. We generated HK-2 cells with stably knocked down FNDC3B expression using effective short-hairpin RNAs. (A) Western blot was performed to assess the FNDC3B knockdown efficiency. (B) Western blotting was performed to assess the expression level of Caspase3 with stably knocked down FNDC3B in HK-2 cells. (C) Semi-quantify FNDC3B. (D) Semi-quantify Caspase3. (E) Flow cytometry analysis of the effect of inhibition of FNDC3B on HK-2 cell apoptosis. (F) Quantitative analysis of the percentage of apoptotic cells by flow cytometry. Data were expressed as mean ± SD ( n = 3). * p
    Figure Legend Snippet: Knockdown of FNDC3B markedly reduced the apoptosis of renal tubular cells. We generated HK-2 cells with stably knocked down FNDC3B expression using effective short-hairpin RNAs. (A) Western blot was performed to assess the FNDC3B knockdown efficiency. (B) Western blotting was performed to assess the expression level of Caspase3 with stably knocked down FNDC3B in HK-2 cells. (C) Semi-quantify FNDC3B. (D) Semi-quantify Caspase3. (E) Flow cytometry analysis of the effect of inhibition of FNDC3B on HK-2 cell apoptosis. (F) Quantitative analysis of the percentage of apoptotic cells by flow cytometry. Data were expressed as mean ± SD ( n = 3). * p

    Techniques Used: Generated, Stable Transfection, Expressing, Western Blot, Flow Cytometry, Inhibition

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    Boster Bio mouse anti caspase 3 p12 subunit monoclonal antibody
    Effects of HFD catch-up growth and liraglutide treatment on β-cell function and apoptosis. Pdx-1 protein (a1) and mRNA (a2) levels of islets at the end of week 8. Pdx-1 protein levels of islets were assessed by Western blot and the results were expressed as the ratios of Pdx-1 and GAPDH. The Pdx-1 mRNA levels were detected by qPCR and expressed as the ratios of Pdx-1 and β-actin. Protein levels of Bcl-2 (b1) and <t>Caspase-3</t> p 12 subunit (c1) were detected by Western blot and the results were expressed as the ratios of Caspase-3 <t>p12</t> subunit or Bcl-2 with GAPDH. The mRNA levels of Bcl-2 (b2) and Procaspase-3 (c2) were expressed as the ratios of Proaspase-3 or Bcl-2 with β-actin. All the results are expressed as mean ± SEM. * p
    Mouse Anti Caspase 3 P12 Subunit Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti caspase 3 p12 subunit monoclonal antibody/product/Boster Bio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti caspase 3 p12 subunit monoclonal antibody - by Bioz Stars, 2022-08
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    94
    Boster Bio cleaved caspase 3
    The effects of anemonin treatment on the homoeostasis of articular cartilage after DMM surgery. Sections of articular cartilage from the mice with or without anemonin treatment after 8 weeks of DMM surgery and sham operation were analysed by immunohistochemical staining ( n = 5 per group). IHC stainings of ( A – D ) MMP 13, ( E – H ) ADAMTS 5, ( I – L ) collagen X, ( M – P ) Aggrecan and ( Q – T ) cleaved <t>caspase‐3</t> were performed in mice after DMM surgery. ( U – X ) The ratios of immunoreactive positive cells, MMP 13 ( U ), ADAMTS 5 ( V ), collagen X ( W ) and cleaved caspase‐3 ( X ) were analysed. Scale bar: 50 μm ( A – T ). Data are expressed as the mean (symbols) ± 95% confidence intervals (error bar).
    Cleaved Caspase 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    91
    Boster Bio rabbit anti rat caspase 3 monoclonal antibody
    <t>Caspase-3</t> expression in retinal ganglial cells (RGCs) in rats (immunofluorescence staining, × 200). Following acute ocular hypertension, Caspase-3 positive expression is detected in the rat retina, mainly in the cytoplasm, and gradually decreased by 2 days. Green fluorescence spots represents caspase-3-positive cells. Fluorescein served as the fluorescent dye.
    Rabbit Anti Rat Caspase 3 Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat caspase 3 monoclonal antibody/product/Boster Bio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat caspase 3 monoclonal antibody - by Bioz Stars, 2022-08
    91/100 stars
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    Effects of HFD catch-up growth and liraglutide treatment on β-cell function and apoptosis. Pdx-1 protein (a1) and mRNA (a2) levels of islets at the end of week 8. Pdx-1 protein levels of islets were assessed by Western blot and the results were expressed as the ratios of Pdx-1 and GAPDH. The Pdx-1 mRNA levels were detected by qPCR and expressed as the ratios of Pdx-1 and β-actin. Protein levels of Bcl-2 (b1) and Caspase-3 p 12 subunit (c1) were detected by Western blot and the results were expressed as the ratios of Caspase-3 p12 subunit or Bcl-2 with GAPDH. The mRNA levels of Bcl-2 (b2) and Procaspase-3 (c2) were expressed as the ratios of Proaspase-3 or Bcl-2 with β-actin. All the results are expressed as mean ± SEM. * p

    Journal: Experimental Biology and Medicine

    Article Title: Liraglutide prevents fast weight gain and β-cell dysfunction in male catch-up growth rats

    doi: 10.1177/1535370214567614

    Figure Lengend Snippet: Effects of HFD catch-up growth and liraglutide treatment on β-cell function and apoptosis. Pdx-1 protein (a1) and mRNA (a2) levels of islets at the end of week 8. Pdx-1 protein levels of islets were assessed by Western blot and the results were expressed as the ratios of Pdx-1 and GAPDH. The Pdx-1 mRNA levels were detected by qPCR and expressed as the ratios of Pdx-1 and β-actin. Protein levels of Bcl-2 (b1) and Caspase-3 p 12 subunit (c1) were detected by Western blot and the results were expressed as the ratios of Caspase-3 p12 subunit or Bcl-2 with GAPDH. The mRNA levels of Bcl-2 (b2) and Procaspase-3 (c2) were expressed as the ratios of Proaspase-3 or Bcl-2 with β-actin. All the results are expressed as mean ± SEM. * p

    Article Snippet: Protein extracts from islets (20 µg from each rat) were subjected to Western blot analysis using mouse antipancreatic duodenal homeobox-1 (Pdx-1) monoclonal antibody (1:800; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Caspase-3 p12 subunit monoclonal antibody (1:500, Boster Bio-Engineering, China), mouse anti-B-cell lymphoma (Bcl-2) polyclonal antibody (1:800, Bioworld, USA), and mouse anti-GAPDH antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Cell Function Assay, Western Blot, Real-time Polymerase Chain Reaction

    The effects of anemonin treatment on the homoeostasis of articular cartilage after DMM surgery. Sections of articular cartilage from the mice with or without anemonin treatment after 8 weeks of DMM surgery and sham operation were analysed by immunohistochemical staining ( n = 5 per group). IHC stainings of ( A – D ) MMP 13, ( E – H ) ADAMTS 5, ( I – L ) collagen X, ( M – P ) Aggrecan and ( Q – T ) cleaved caspase‐3 were performed in mice after DMM surgery. ( U – X ) The ratios of immunoreactive positive cells, MMP 13 ( U ), ADAMTS 5 ( V ), collagen X ( W ) and cleaved caspase‐3 ( X ) were analysed. Scale bar: 50 μm ( A – T ). Data are expressed as the mean (symbols) ± 95% confidence intervals (error bar).

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL‐1β/ NF‐κB pathway

    doi: 10.1111/jcmm.13227

    Figure Lengend Snippet: The effects of anemonin treatment on the homoeostasis of articular cartilage after DMM surgery. Sections of articular cartilage from the mice with or without anemonin treatment after 8 weeks of DMM surgery and sham operation were analysed by immunohistochemical staining ( n = 5 per group). IHC stainings of ( A – D ) MMP 13, ( E – H ) ADAMTS 5, ( I – L ) collagen X, ( M – P ) Aggrecan and ( Q – T ) cleaved caspase‐3 were performed in mice after DMM surgery. ( U – X ) The ratios of immunoreactive positive cells, MMP 13 ( U ), ADAMTS 5 ( V ), collagen X ( W ) and cleaved caspase‐3 ( X ) were analysed. Scale bar: 50 μm ( A – T ). Data are expressed as the mean (symbols) ± 95% confidence intervals (error bar).

    Article Snippet: Primary antibodies against the following proteins were used: collagen II (1:400; Chondrex, Redmond, WA, USA), collagen X (1:200; Abcam, Cambridge, MA, USA), Aggrecan (1:200 Abcam), MMP13 (1:200; PeproTech, Chicago, IL, USA), ADAMTS5 (1:200; Abcam), cleaved caspase‐3 (1:100; Boster, China) and phosphor‐P65 (1:200; CST, Danvers, MA, USA).

    Techniques: Mouse Assay, Immunohistochemistry, Staining

    Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved caspase-3 and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.

    Journal: Oncology Letters

    Article Title: Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy

    doi: 10.3892/ol.2018.9584

    Figure Lengend Snippet: Cell apoptosis was decreased by HBV-associated exosomes in a concentration-dependent manner. (A) Cells were treated with 1×10 10 , 2×10 10 or 4×10 10 HBV-associated exosomes, and the expression of cleaved caspase-3 and Bcl-2 was assessed by western blotting. (B) Flow cytometry results indicated that cell apoptosis was negatively associated with the concentration of HBV-associated exosomes. HBV, hepatitis B virus; Bcl-2, B-cell lymphoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Exo, exosome; Oxa, oxaliplatin; UR, upper right; LR, lower right; Ctr, control; PBS, phosphate-buffered saline.

    Article Snippet: The primary antibody against cleaved caspase-3 (dilution, 1:500; catalog no. PB0183) was obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China), the anti-B-cell lymphoma-2 (Bcl-2) antibody (dilution, 1:500; catalog no. WL01556) was obtained from Wanleibio Co., Ltd. (Shanghai, China) and the antibody against GAPDH (dilution, 1:5,000; catalog no. 5174S) was obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Concentration Assay, Expressing, Western Blot, Flow Cytometry, Cytometry

    Caspase-3 expression in retinal ganglial cells (RGCs) in rats (immunofluorescence staining, × 200). Following acute ocular hypertension, Caspase-3 positive expression is detected in the rat retina, mainly in the cytoplasm, and gradually decreased by 2 days. Green fluorescence spots represents caspase-3-positive cells. Fluorescein served as the fluorescent dye.

    Journal: Neural Regeneration Research

    Article Title: Alpha B-crystallin improved survival of retinal ganglion cells in a rat model of acute ocular hypertension ☆

    doi: 10.3969/j.issn.1673-5374.2012.19.008

    Figure Lengend Snippet: Caspase-3 expression in retinal ganglial cells (RGCs) in rats (immunofluorescence staining, × 200). Following acute ocular hypertension, Caspase-3 positive expression is detected in the rat retina, mainly in the cytoplasm, and gradually decreased by 2 days. Green fluorescence spots represents caspase-3-positive cells. Fluorescein served as the fluorescent dye.

    Article Snippet: Briefly, the retina was blocked in 3% bovine serum albumin at 4°C for 1 hour, incubated with rabbit anti-rat caspase-3 monoclonal antibody (1:500; Wuhan Boster Biotechnology, Wuhan, China) at 4°C for 12 hours, followed by fluorescein-labeled goat anti-rabbit IgG (1:200; Wuhan Boster Biotechnology) at 4°C for 2 hours.

    Techniques: Expressing, Immunofluorescence, Staining, Fluorescence

    αB-crystallin decreased caspase-3 expression in a rat model of acute ocular hypertension

    Journal: Neural Regeneration Research

    Article Title: Alpha B-crystallin improved survival of retinal ganglion cells in a rat model of acute ocular hypertension ☆

    doi: 10.3969/j.issn.1673-5374.2012.19.008

    Figure Lengend Snippet: αB-crystallin decreased caspase-3 expression in a rat model of acute ocular hypertension

    Article Snippet: Briefly, the retina was blocked in 3% bovine serum albumin at 4°C for 1 hour, incubated with rabbit anti-rat caspase-3 monoclonal antibody (1:500; Wuhan Boster Biotechnology, Wuhan, China) at 4°C for 12 hours, followed by fluorescein-labeled goat anti-rabbit IgG (1:200; Wuhan Boster Biotechnology) at 4°C for 2 hours.

    Techniques: Expressing