Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK

doi: 10.12659/MSM.912862

Figure Lengend Snippet: Primary antibodies used in the present study.

Article Snippet: Rabbit anti-Cacna1b , 1: 1000 , Alomone Labs, Jerusalem, Israel.

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK

doi: 10.12659/MSM.912862

Figure Lengend Snippet: Effects of knockdown ACAT1 on CACNAs, PKC, and ERK phosphorylation and in Aβ-induced SH-SY5Y cells. ( A ) Western blot results showed that the relative expression of CACNA1b and CACNA2d1 was increased significantly, while the total PKC and the ratio of phospho-ERK (p-ERK)/ERK were decreased significantly after Aβ 25-35 treatment. ( B ) Knockdown of ACAT1 rescued the reduction of PKC and phosphorylation level of ERK, and also retarded the upregulation of CACNA1b and CACNA2d1 induced by Aβ. (** P <0.01 and * P <0.05).

Article Snippet: Rabbit anti-Cacna1b , 1: 1000 , Alomone Labs, Jerusalem, Israel.

Techniques: Western Blot, Expressing