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rabbit anti ca9  (Cloud-Clone corp)

 
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    Structured Review

    Cloud-Clone corp rabbit anti ca9
    (A) The selected lncRNAs possess multiple HIF1α binding motifs (R/CGTG) within their promoter regions, as illustrated in the schematic. (B) Western blot shows the addition of CoCl 2 led to elevated expression of HIFα which was reduced after PX-478 treatment. (C) The RT–qPCR analysis of cytoplasmic RNA from the above treatment groups showed that treating U2OS cells with the HIF1α-specific inhibitor PX-478 under hypoxic conditions led to a significant decrease in the expression of LUCAT1-211 and MSC-AS1-212. <t>CA9</t> serves as a known hypoxia-induced gene regulated by HIF1α. Values are expressed as mean ± standard deviation (SD) from three biological replicates. Statistical significance is denoted as follows: ns indicates non-significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001, with n ≥ 3.
    Rabbit Anti Ca9, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Identification of transcriptome-wide cobalt chloride-induced hypoxia-responsive long noncoding RNAs regulated by cytoplasmic mRNA capping enzyme"

    Article Title: Identification of transcriptome-wide cobalt chloride-induced hypoxia-responsive long noncoding RNAs regulated by cytoplasmic mRNA capping enzyme

    Journal: bioRxiv

    doi: 10.1101/2024.11.21.624636

    (A) The selected lncRNAs possess multiple HIF1α binding motifs (R/CGTG) within their promoter regions, as illustrated in the schematic. (B) Western blot shows the addition of CoCl 2 led to elevated expression of HIFα which was reduced after PX-478 treatment. (C) The RT–qPCR analysis of cytoplasmic RNA from the above treatment groups showed that treating U2OS cells with the HIF1α-specific inhibitor PX-478 under hypoxic conditions led to a significant decrease in the expression of LUCAT1-211 and MSC-AS1-212. CA9 serves as a known hypoxia-induced gene regulated by HIF1α. Values are expressed as mean ± standard deviation (SD) from three biological replicates. Statistical significance is denoted as follows: ns indicates non-significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001, with n ≥ 3.
    Figure Legend Snippet: (A) The selected lncRNAs possess multiple HIF1α binding motifs (R/CGTG) within their promoter regions, as illustrated in the schematic. (B) Western blot shows the addition of CoCl 2 led to elevated expression of HIFα which was reduced after PX-478 treatment. (C) The RT–qPCR analysis of cytoplasmic RNA from the above treatment groups showed that treating U2OS cells with the HIF1α-specific inhibitor PX-478 under hypoxic conditions led to a significant decrease in the expression of LUCAT1-211 and MSC-AS1-212. CA9 serves as a known hypoxia-induced gene regulated by HIF1α. Values are expressed as mean ± standard deviation (SD) from three biological replicates. Statistical significance is denoted as follows: ns indicates non-significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001, with n ≥ 3.

    Techniques Used: Binding Assay, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation



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    (A) The selected lncRNAs possess multiple HIF1α binding motifs (R/CGTG) within their promoter regions, as illustrated in the schematic. (B) Western blot shows the addition of CoCl 2 led to elevated expression of HIFα which was reduced after PX-478 treatment. (C) The RT–qPCR analysis of cytoplasmic RNA from the above treatment groups showed that treating U2OS cells with the HIF1α-specific inhibitor PX-478 under hypoxic conditions led to a significant decrease in the expression of LUCAT1-211 and MSC-AS1-212. <t>CA9</t> serves as a known hypoxia-induced gene regulated by HIF1α. Values are expressed as mean ± standard deviation (SD) from three biological replicates. Statistical significance is denoted as follows: ns indicates non-significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001, with n ≥ 3.
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    Image Search Results


    (A) The selected lncRNAs possess multiple HIF1α binding motifs (R/CGTG) within their promoter regions, as illustrated in the schematic. (B) Western blot shows the addition of CoCl 2 led to elevated expression of HIFα which was reduced after PX-478 treatment. (C) The RT–qPCR analysis of cytoplasmic RNA from the above treatment groups showed that treating U2OS cells with the HIF1α-specific inhibitor PX-478 under hypoxic conditions led to a significant decrease in the expression of LUCAT1-211 and MSC-AS1-212. CA9 serves as a known hypoxia-induced gene regulated by HIF1α. Values are expressed as mean ± standard deviation (SD) from three biological replicates. Statistical significance is denoted as follows: ns indicates non-significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001, with n ≥ 3.

    Journal: bioRxiv

    Article Title: Identification of transcriptome-wide cobalt chloride-induced hypoxia-responsive long noncoding RNAs regulated by cytoplasmic mRNA capping enzyme

    doi: 10.1101/2024.11.21.624636

    Figure Lengend Snippet: (A) The selected lncRNAs possess multiple HIF1α binding motifs (R/CGTG) within their promoter regions, as illustrated in the schematic. (B) Western blot shows the addition of CoCl 2 led to elevated expression of HIFα which was reduced after PX-478 treatment. (C) The RT–qPCR analysis of cytoplasmic RNA from the above treatment groups showed that treating U2OS cells with the HIF1α-specific inhibitor PX-478 under hypoxic conditions led to a significant decrease in the expression of LUCAT1-211 and MSC-AS1-212. CA9 serves as a known hypoxia-induced gene regulated by HIF1α. Values are expressed as mean ± standard deviation (SD) from three biological replicates. Statistical significance is denoted as follows: ns indicates non-significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001, with n ≥ 3.

    Article Snippet: The primary antibodies used included: 1:1000 rabbit anti-HIF1α (Cell Signaling Technology, cat no# 36169), 1:1000 mouse anti-β actin (Santa Cruz Biotechnology, cat no# SC-517582), 1:1000 rabbit anti-CE (Novus Biologicals, cat no# NPB1-49973), 1:2000 mouse anti-GAPDH (Novus Biologicals, cat no# 2D4A7), 1:2000 rabbit anti-CA9 (Cloud-clone Corp, cat no# PAD076Hu01), 1:2000 mouse anti-lamin (DHSB, cat no# MANLAC1(4A7)), and anti-Xrn1 (cat no# PA5-57110).

    Techniques: Binding Assay, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation