rabbit anti aqp4  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti aqp4
    Interlaminar astrocytes in the mouse cortex express canonical astrocytic markers. Sections from 9‐month‐old mouse engrafted with RFP expressing hiPSC‐astrocytes and immunostained with the antibodies against hGFAP, hCD44, S100B, Kir4.1, and <t>AQP4.</t> Scale = 10 μm [Color figure can be viewed at wileyonlinelibrary.com ]
    Rabbit Anti Aqp4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti aqp4/product/Alomone Labs
    Average 93 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    rabbit anti aqp4 - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "Modeling human‐specific interlaminar astrocytes in the mouse cerebral cortex. Modeling human‐specific interlaminar astrocytes in the mouse cerebral cortex"

    Article Title: Modeling human‐specific interlaminar astrocytes in the mouse cerebral cortex. Modeling human‐specific interlaminar astrocytes in the mouse cerebral cortex

    Journal: The Journal of Comparative Neurology

    doi: 10.1002/cne.24979

    Interlaminar astrocytes in the mouse cortex express canonical astrocytic markers. Sections from 9‐month‐old mouse engrafted with RFP expressing hiPSC‐astrocytes and immunostained with the antibodies against hGFAP, hCD44, S100B, Kir4.1, and AQP4. Scale = 10 μm [Color figure can be viewed at wileyonlinelibrary.com ]
    Figure Legend Snippet: Interlaminar astrocytes in the mouse cortex express canonical astrocytic markers. Sections from 9‐month‐old mouse engrafted with RFP expressing hiPSC‐astrocytes and immunostained with the antibodies against hGFAP, hCD44, S100B, Kir4.1, and AQP4. Scale = 10 μm [Color figure can be viewed at wileyonlinelibrary.com ]

    Techniques Used: Expressing

    2) Product Images from "Enhanced Expression of Markers for Astrocytes in the Brain of a Line of GFAP-TK Transgenic Mice"

    Article Title: Enhanced Expression of Markers for Astrocytes in the Brain of a Line of GFAP-TK Transgenic Mice

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2017.00212

    The expression of AQP4 was increased in the cortex and hippocampus of 3-month-old TK-1 mice. (A) Representative photomicrographs of AQP4 expression in the cortex and hippocampus of 3-month-old TK-1 and TK-2 mice and their age-matched controls. Scale bar, 200 μm. (B,C) Fluorescence intensity was quantified by using image-analysis software in the cortex and hippocampus. ( n = 3 mice per group, 3 brain slices for each mouse). (D) Protein bands of AQP4 in the cortex and hippocampus, GAPDH severed as the loading control. (E) Quantification of the level of AQP4 in the cortex of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). (F) Quantification of the level of AQP4 in the hippocampus of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). Data represent mean ± SEM, * P
    Figure Legend Snippet: The expression of AQP4 was increased in the cortex and hippocampus of 3-month-old TK-1 mice. (A) Representative photomicrographs of AQP4 expression in the cortex and hippocampus of 3-month-old TK-1 and TK-2 mice and their age-matched controls. Scale bar, 200 μm. (B,C) Fluorescence intensity was quantified by using image-analysis software in the cortex and hippocampus. ( n = 3 mice per group, 3 brain slices for each mouse). (D) Protein bands of AQP4 in the cortex and hippocampus, GAPDH severed as the loading control. (E) Quantification of the level of AQP4 in the cortex of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). (F) Quantification of the level of AQP4 in the hippocampus of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). Data represent mean ± SEM, * P

    Techniques Used: Expressing, Mouse Assay, Fluorescence, Software

    3) Product Images from "Enhanced Expression of Markers for Astrocytes in the Brain of a Line of GFAP-TK Transgenic Mice"

    Article Title: Enhanced Expression of Markers for Astrocytes in the Brain of a Line of GFAP-TK Transgenic Mice

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2017.00212

    The expression of AQP4 was increased in the cortex and hippocampus of 3-month-old TK-1 mice. (A) Representative photomicrographs of AQP4 expression in the cortex and hippocampus of 3-month-old TK-1 and TK-2 mice and their age-matched controls. Scale bar, 200 μm. (B,C) Fluorescence intensity was quantified by using image-analysis software in the cortex and hippocampus. ( n = 3 mice per group, 3 brain slices for each mouse). (D) Protein bands of AQP4 in the cortex and hippocampus, GAPDH severed as the loading control. (E) Quantification of the level of AQP4 in the cortex of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). (F) Quantification of the level of AQP4 in the hippocampus of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). Data represent mean ± SEM, * P
    Figure Legend Snippet: The expression of AQP4 was increased in the cortex and hippocampus of 3-month-old TK-1 mice. (A) Representative photomicrographs of AQP4 expression in the cortex and hippocampus of 3-month-old TK-1 and TK-2 mice and their age-matched controls. Scale bar, 200 μm. (B,C) Fluorescence intensity was quantified by using image-analysis software in the cortex and hippocampus. ( n = 3 mice per group, 3 brain slices for each mouse). (D) Protein bands of AQP4 in the cortex and hippocampus, GAPDH severed as the loading control. (E) Quantification of the level of AQP4 in the cortex of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). (F) Quantification of the level of AQP4 in the hippocampus of 3-month-old TK-1 ( n = 7 mice) and TK-2 mice ( n = 4 mice) and their age-matched controls ( n = 4 mice per group). Data represent mean ± SEM, * P

    Techniques Used: Expressing, Mouse Assay, Fluorescence, Software

    4) Product Images from "Characterization of AQPs in Mouse, Rat, and Human Colon and Their Selective Regulation by Bile Acids"

    Article Title: Characterization of AQPs in Mouse, Rat, and Human Colon and Their Selective Regulation by Bile Acids

    Journal: Frontiers in Nutrition

    doi: 10.3389/fnut.2016.00046

    Immunohistochemistry of AQP4 in rat, mouse, and human colon sections . (A,B) Proximal rat colon. (D,E) Distal rat colon. (C–F) Rat kidney. (G,H) Proximal mouse colon. (J,K) Distal mouse colon. (I–L) Mouse kidney. (M,N) Proximal human colon. (P,Q) Distal human colon. (O–R) Human kidney. Scalebar = 100 μm.
    Figure Legend Snippet: Immunohistochemistry of AQP4 in rat, mouse, and human colon sections . (A,B) Proximal rat colon. (D,E) Distal rat colon. (C–F) Rat kidney. (G,H) Proximal mouse colon. (J,K) Distal mouse colon. (I–L) Mouse kidney. (M,N) Proximal human colon. (P,Q) Distal human colon. (O–R) Human kidney. Scalebar = 100 μm.

    Techniques Used: Immunohistochemistry

    5) Product Images from "Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats"

    Article Title: Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats

    Journal: International Journal of Clinical and Experimental Medicine

    doi:

    Ulinastatin down-regulates the expression level of AQP4 in cerebral ischemia-reperfusion. Date are expressed as Mean ± S.E.M (n = 5). IR group vs. S group, P
    Figure Legend Snippet: Ulinastatin down-regulates the expression level of AQP4 in cerebral ischemia-reperfusion. Date are expressed as Mean ± S.E.M (n = 5). IR group vs. S group, P

    Techniques Used: Expressing

    Representative western blotting of AQP4 expression. A: Sham (S) group; B: Ischemia-reperfusion (IR) 6 h group; C: Ulinastatin (U) 6 h group; D: IR 24 h group; E: U 24 h group; F: IR 48 h group; G: U 48 h group.
    Figure Legend Snippet: Representative western blotting of AQP4 expression. A: Sham (S) group; B: Ischemia-reperfusion (IR) 6 h group; C: Ulinastatin (U) 6 h group; D: IR 24 h group; E: U 24 h group; F: IR 48 h group; G: U 48 h group.

    Techniques Used: Western Blot, Expressing

    Immunohistochemistry of Aquaporin-4 (AQP4) expressions in ependyma, choroid plexus and blood capillary. A: Sham (S) group. B: Ischemia-reperfusion (IR) 6 h group. C: Ulinastatin (U) 6 h group. D: IR 24 h group. E: U 24 h group. F: IR 48 h group. G: U
    Figure Legend Snippet: Immunohistochemistry of Aquaporin-4 (AQP4) expressions in ependyma, choroid plexus and blood capillary. A: Sham (S) group. B: Ischemia-reperfusion (IR) 6 h group. C: Ulinastatin (U) 6 h group. D: IR 24 h group. E: U 24 h group. F: IR 48 h group. G: U

    Techniques Used: Immunohistochemistry

    6) Product Images from "A High Throughput Screen Identifies Chemical Modulators of the Laminin-Induced Clustering of Dystroglycan and Aquaporin-4 in Primary Astrocytes"

    Article Title: A High Throughput Screen Identifies Chemical Modulators of the Laminin-Induced Clustering of Dystroglycan and Aquaporin-4 in Primary Astrocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0017559

    Effect of chloranil on the laminin-induced co-clustering of ß-DG and AQP4. Primary astrocytes were treated for 7 h with 20 nM laminin and15 µM chloranil during the last 4 h. The concentration of chloranil varied from 0 (A,B,C), 6(D,E,F), 12(G,H,I), 25 (J,K,L), 50 (M,N, O) to 100 µM (P,Q,R). The cells were fixed and labelled for μ-DG (A, D, G, J, M and P) and AQP4 (B, E, H, K, N and Q). Clustered staining was quantified using confocal microscopy. Scale bar, 30 µm.
    Figure Legend Snippet: Effect of chloranil on the laminin-induced co-clustering of ß-DG and AQP4. Primary astrocytes were treated for 7 h with 20 nM laminin and15 µM chloranil during the last 4 h. The concentration of chloranil varied from 0 (A,B,C), 6(D,E,F), 12(G,H,I), 25 (J,K,L), 50 (M,N, O) to 100 µM (P,Q,R). The cells were fixed and labelled for μ-DG (A, D, G, J, M and P) and AQP4 (B, E, H, K, N and Q). Clustered staining was quantified using confocal microscopy. Scale bar, 30 µm.

    Techniques Used: Concentration Assay, Staining, Confocal Microscopy

    Time-dependent shedding of ß-dystroglycan by chloranil is blocked by the metalloproteinase inhibitor, prinomastat. Primary astrocytes were incubated with 25 µM chloranil for 4 or 7 h with or without addition of 15 nM or 30 nM prinomastat. Extracted proteins were loaded (30 µg/lane) and analyzed for ß-DG and AQP4 expression levels by western blot analysis. Note the increase in signal intensity of the 31 kDaß-DG band after extended incubation with chloranil and its disappearance with prinomastat co-incubation.
    Figure Legend Snippet: Time-dependent shedding of ß-dystroglycan by chloranil is blocked by the metalloproteinase inhibitor, prinomastat. Primary astrocytes were incubated with 25 µM chloranil for 4 or 7 h with or without addition of 15 nM or 30 nM prinomastat. Extracted proteins were loaded (30 µg/lane) and analyzed for ß-DG and AQP4 expression levels by western blot analysis. Note the increase in signal intensity of the 31 kDaß-DG band after extended incubation with chloranil and its disappearance with prinomastat co-incubation.

    Techniques Used: Incubation, Expressing, Western Blot

    Effect of chloranil and flunarizine on astrocyte survival and ß-dystroglycan, and AQP4 expression. A. Primary astrocytes were incubated for 4 h with 15 µM of active chemicals. Extracted proteins were loaded (30 µg/lane) and analyzed for ß-DG, syntrophin and AQP4 expression levels by western blot analysis. Note the 31 kDa band under the 42 kDa band corresponding to the cleaved form of ß-dystroglycan upon chloranil treatment. B. Primary astrocytes were incubated for 4 h with different concentrations of the active chemicals. Chemicals and media were washed away and the cells were assayed for cell viability by MTT assay.
    Figure Legend Snippet: Effect of chloranil and flunarizine on astrocyte survival and ß-dystroglycan, and AQP4 expression. A. Primary astrocytes were incubated for 4 h with 15 µM of active chemicals. Extracted proteins were loaded (30 µg/lane) and analyzed for ß-DG, syntrophin and AQP4 expression levels by western blot analysis. Note the 31 kDa band under the 42 kDa band corresponding to the cleaved form of ß-dystroglycan upon chloranil treatment. B. Primary astrocytes were incubated for 4 h with different concentrations of the active chemicals. Chemicals and media were washed away and the cells were assayed for cell viability by MTT assay.

    Techniques Used: Expressing, Incubation, Western Blot, MTT Assay

    7) Product Images from "Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain"

    Article Title: Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain

    Journal: Cerebrospinal Fluid Research

    doi: 10.1186/1743-8454-7-20

    Correlation between quantitative MRI and relative AQP4 expression . Plots of lateral ventricular volume vs. relative aquaporin-4 expression (A) and periventricular apparent diffusion coefficient value vs. relative aquaporin-4 expression (B) in both controls, hydrocephalic (two days, 2 D, one week,1W and two weeks, 2W) and non-hydrocephalic kaolin-injected animals. In hydrocephalic animals we found a significant linear positive correlation between lateral ventricular volume and periventricular aquaporin-4 expression (r 2 = 0.33, p = 0.002). No correlation between periventricular apparent diffusion coefficient value and relative aquaporin-4 expression was found.
    Figure Legend Snippet: Correlation between quantitative MRI and relative AQP4 expression . Plots of lateral ventricular volume vs. relative aquaporin-4 expression (A) and periventricular apparent diffusion coefficient value vs. relative aquaporin-4 expression (B) in both controls, hydrocephalic (two days, 2 D, one week,1W and two weeks, 2W) and non-hydrocephalic kaolin-injected animals. In hydrocephalic animals we found a significant linear positive correlation between lateral ventricular volume and periventricular aquaporin-4 expression (r 2 = 0.33, p = 0.002). No correlation between periventricular apparent diffusion coefficient value and relative aquaporin-4 expression was found.

    Techniques Used: Magnetic Resonance Imaging, Expressing, Diffusion-based Assay, Injection

    8) Product Images from "Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain"

    Article Title: Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain

    Journal: Cerebrospinal Fluid Research

    doi: 10.1186/1743-8454-7-20

    Correlation between quantitative MRI and relative AQP4 expression . Plots of lateral ventricular volume vs. relative aquaporin-4 expression (A) and periventricular apparent diffusion coefficient value vs. relative aquaporin-4 expression (B) in both controls, hydrocephalic (two days, 2 D, one week,1W and two weeks, 2W) and non-hydrocephalic kaolin-injected animals. In hydrocephalic animals we found a significant linear positive correlation between lateral ventricular volume and periventricular aquaporin-4 expression (r 2 = 0.33, p = 0.002). No correlation between periventricular apparent diffusion coefficient value and relative aquaporin-4 expression was found.
    Figure Legend Snippet: Correlation between quantitative MRI and relative AQP4 expression . Plots of lateral ventricular volume vs. relative aquaporin-4 expression (A) and periventricular apparent diffusion coefficient value vs. relative aquaporin-4 expression (B) in both controls, hydrocephalic (two days, 2 D, one week,1W and two weeks, 2W) and non-hydrocephalic kaolin-injected animals. In hydrocephalic animals we found a significant linear positive correlation between lateral ventricular volume and periventricular aquaporin-4 expression (r 2 = 0.33, p = 0.002). No correlation between periventricular apparent diffusion coefficient value and relative aquaporin-4 expression was found.

    Techniques Used: Magnetic Resonance Imaging, Expressing, Diffusion-based Assay, Injection

    9) Product Images from "Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain"

    Article Title: Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain

    Journal: Cerebrospinal Fluid Research

    doi: 10.1186/1743-8454-7-20

    Correlation between quantitative MRI and relative AQP4 expression . Plots of lateral ventricular volume vs. relative aquaporin-4 expression (A) and periventricular apparent diffusion coefficient value vs. relative aquaporin-4 expression (B) in both controls, hydrocephalic (two days, 2 D, one week,1W and two weeks, 2W) and non-hydrocephalic kaolin-injected animals. In hydrocephalic animals we found a significant linear positive correlation between lateral ventricular volume and periventricular aquaporin-4 expression (r 2 = 0.33, p = 0.002). No correlation between periventricular apparent diffusion coefficient value and relative aquaporin-4 expression was found.
    Figure Legend Snippet: Correlation between quantitative MRI and relative AQP4 expression . Plots of lateral ventricular volume vs. relative aquaporin-4 expression (A) and periventricular apparent diffusion coefficient value vs. relative aquaporin-4 expression (B) in both controls, hydrocephalic (two days, 2 D, one week,1W and two weeks, 2W) and non-hydrocephalic kaolin-injected animals. In hydrocephalic animals we found a significant linear positive correlation between lateral ventricular volume and periventricular aquaporin-4 expression (r 2 = 0.33, p = 0.002). No correlation between periventricular apparent diffusion coefficient value and relative aquaporin-4 expression was found.

    Techniques Used: Magnetic Resonance Imaging, Expressing, Diffusion-based Assay, Injection

    10) Product Images from "AQUAPORIN-4 INDEPENDENT Kir4.1 K+ CHANNEL FUNCTION IN BRAIN GLIAL CELLS"

    Article Title: AQUAPORIN-4 INDEPENDENT Kir4.1 K+ CHANNEL FUNCTION IN BRAIN GLIAL CELLS

    Journal: Molecular and cellular neurosciences

    doi: 10.1016/j.mcn.2007.08.007

    Comparable whole-cell K + currents in astroglial cells from wild-type and AQP4 null mice
    Figure Legend Snippet: Comparable whole-cell K + currents in astroglial cells from wild-type and AQP4 null mice

    Techniques Used: Mouse Assay

    Kir4.1 inhibition or knock-down does not affect AQP4 water permeability
    Figure Legend Snippet: Kir4.1 inhibition or knock-down does not affect AQP4 water permeability

    Techniques Used: Inhibition, Permeability

    Kir4.1 and AQP4 expression in mouse brain and isolated astroglial cells
    Figure Legend Snippet: Kir4.1 and AQP4 expression in mouse brain and isolated astroglial cells

    Techniques Used: Expressing, Isolation

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  • 94
    Alomone Labs antibodies for aqp4
    CTBS improved <t>AQP4</t> polarization in APP/PS1 mice. (a) Immunofluorescent staining of AQP4 in the cortex and hippocampus (200×, scale bar: 100 μ m). (b) Statistical analysis of relative AQP4 expression levels in the cortex. (c) Statistical analysis of relative AQP4 expression levels in the hippocampus. (d) Statistical analysis of relative perivascular polarization of AQP4 in the cortex. (e) Statistical analysis of relative perivascular polarization of AQP4 in the hippocampus. Data are presented as the mean ± SD ( n = 6 mice per group, unpaired t -test for comparing two individual groups; ∗ P
    Antibodies For Aqp4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies for aqp4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies for aqp4 - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    95
    Alomone Labs rabbit anti aqp 4 igg
    Gliosis in the hippocampal region of CKD model rats. Diagram of the rat hippocampus ( A ). GFAP immunoreactivity in the hippocampus of CKD 10-wk rats was enhanced in the CA1 and DG regions (arrows) compared with naïve rats ( B1 – B4 ): scale bar = 25 μm. The average number of GFAP-positive astroglia and the densitometric analysis results of GFAP immunoreactivity were consistent with the immunohistochemical data ( C1 – C4 ). Double labeling of GFAP and <t>AQP-4</t> in the hippocampal CA1 and DG regions of naïve rats ( D , F ) and CKD 10-wk rats ( E , G ): GFAP (green); AQP-4 (red); merged images (yellow); scale bar = 18.8 μm; A.U., arbitrary unit. Data are presented as the mean and SEM. Significant differences from naïve rats ( H1 , H2 ), ** p
    Rabbit Anti Aqp 4 Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti aqp 4 igg/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti aqp 4 igg - by Bioz Stars, 2022-11
    95/100 stars
      Buy from Supplier

    94
    Alomone Labs anti aquaporin 1 antibody
    Gliosis in the hippocampal region of CKD model rats. Diagram of the rat hippocampus ( A ). GFAP immunoreactivity in the hippocampus of CKD 10-wk rats was enhanced in the CA1 and DG regions (arrows) compared with naïve rats ( B1 – B4 ): scale bar = 25 μm. The average number of GFAP-positive astroglia and the densitometric analysis results of GFAP immunoreactivity were consistent with the immunohistochemical data ( C1 – C4 ). Double labeling of GFAP and <t>AQP-4</t> in the hippocampal CA1 and DG regions of naïve rats ( D , F ) and CKD 10-wk rats ( E , G ): GFAP (green); AQP-4 (red); merged images (yellow); scale bar = 18.8 μm; A.U., arbitrary unit. Data are presented as the mean and SEM. Significant differences from naïve rats ( H1 , H2 ), ** p
    Anti Aquaporin 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti aquaporin 1 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti aquaporin 1 antibody - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    CTBS improved AQP4 polarization in APP/PS1 mice. (a) Immunofluorescent staining of AQP4 in the cortex and hippocampus (200×, scale bar: 100 μ m). (b) Statistical analysis of relative AQP4 expression levels in the cortex. (c) Statistical analysis of relative AQP4 expression levels in the hippocampus. (d) Statistical analysis of relative perivascular polarization of AQP4 in the cortex. (e) Statistical analysis of relative perivascular polarization of AQP4 in the hippocampus. Data are presented as the mean ± SD ( n = 6 mice per group, unpaired t -test for comparing two individual groups; ∗ P

    Journal: Mediators of Inflammation

    Article Title: Continuous Theta-Burst Stimulation Promotes Paravascular CSF-Interstitial Fluid Exchange through Regulation of Aquaporin-4 Polarization in APP/PS1 Mice

    doi: 10.1155/2022/2140524

    Figure Lengend Snippet: CTBS improved AQP4 polarization in APP/PS1 mice. (a) Immunofluorescent staining of AQP4 in the cortex and hippocampus (200×, scale bar: 100 μ m). (b) Statistical analysis of relative AQP4 expression levels in the cortex. (c) Statistical analysis of relative AQP4 expression levels in the hippocampus. (d) Statistical analysis of relative perivascular polarization of AQP4 in the cortex. (e) Statistical analysis of relative perivascular polarization of AQP4 in the hippocampus. Data are presented as the mean ± SD ( n = 6 mice per group, unpaired t -test for comparing two individual groups; ∗ P

    Article Snippet: Antibodies for AQP4 (Alomone Labs, 300-314; Jerusalem, Israel) and glial fibrillary acidic protein (GFAP, Sigma, G3893, USA) were used to detect aquaporins and astrocytes.

    Techniques: Mouse Assay, Staining, Expressing

    Gliosis in the hippocampal region of CKD model rats. Diagram of the rat hippocampus ( A ). GFAP immunoreactivity in the hippocampus of CKD 10-wk rats was enhanced in the CA1 and DG regions (arrows) compared with naïve rats ( B1 – B4 ): scale bar = 25 μm. The average number of GFAP-positive astroglia and the densitometric analysis results of GFAP immunoreactivity were consistent with the immunohistochemical data ( C1 – C4 ). Double labeling of GFAP and AQP-4 in the hippocampal CA1 and DG regions of naïve rats ( D , F ) and CKD 10-wk rats ( E , G ): GFAP (green); AQP-4 (red); merged images (yellow); scale bar = 18.8 μm; A.U., arbitrary unit. Data are presented as the mean and SEM. Significant differences from naïve rats ( H1 , H2 ), ** p

    Journal: Brain Sciences

    Article Title: Cognitive Sequelae and Hippocampal Dysfunction in Chronic Kidney Disease following 5/6 Nephrectomy

    doi: 10.3390/brainsci12070905

    Figure Lengend Snippet: Gliosis in the hippocampal region of CKD model rats. Diagram of the rat hippocampus ( A ). GFAP immunoreactivity in the hippocampus of CKD 10-wk rats was enhanced in the CA1 and DG regions (arrows) compared with naïve rats ( B1 – B4 ): scale bar = 25 μm. The average number of GFAP-positive astroglia and the densitometric analysis results of GFAP immunoreactivity were consistent with the immunohistochemical data ( C1 – C4 ). Double labeling of GFAP and AQP-4 in the hippocampal CA1 and DG regions of naïve rats ( D , F ) and CKD 10-wk rats ( E , G ): GFAP (green); AQP-4 (red); merged images (yellow); scale bar = 18.8 μm; A.U., arbitrary unit. Data are presented as the mean and SEM. Significant differences from naïve rats ( H1 , H2 ), ** p

    Article Snippet: Brain tissues were incubated overnight at 4 °C in a mixture of mouse anti-GFAP IgG (Millipore; diluted 1:500) and rabbit anti-AQP-4 IgG (Alomone Labs, Jerusalem, Israel; diluted 1:200).

    Techniques: Immunohistochemistry, Labeling

    Semi-quantification of aquaporin-4 labeling in 6 regions of porcine ONH region. Values in choroid, sclera, and lamina are minimal, not significantly different from each other, while each was significantly lower than retina, myelin transition zone (MTZ), and myelinated optic nerve (MON). Mean ± standard errors. Dotted line identifies the AQP4 background level in choroid. †p

    Journal: PLoS ONE

    Article Title: Aquaporin 4 is not present in normal porcine and human lamina cribrosa

    doi: 10.1371/journal.pone.0268541

    Figure Lengend Snippet: Semi-quantification of aquaporin-4 labeling in 6 regions of porcine ONH region. Values in choroid, sclera, and lamina are minimal, not significantly different from each other, while each was significantly lower than retina, myelin transition zone (MTZ), and myelinated optic nerve (MON). Mean ± standard errors. Dotted line identifies the AQP4 background level in choroid. †p

    Article Snippet: The transferred membrane was blocked for 1 h at room temperature with TBS buffer containing 5% non-fat dry milk followed by incubation with rabbit anti-AQP4 primary antibody (1:1000, Alamone labs) overnight on a shaker at 4°C.

    Techniques: Labeling

    Longitudinal cryosections of immunolabeled aquaporin-4 and phalloidin on human (A-E), and porcine (B) optic nerve head tissue. Minimal label for AQP4 (green) is visible in the lamina cribrosa (between dotted white lines in A, B; seen at higher power in C and E. AQP4 label is prominent in the retinal nerve fiber layer, prelaminar area and myelinated optic nerve in each species. Phalloidin labeling of F-actin (red) is prominent in the prelamina, lamina and myelinated ON (D, E). AQP4 labeling is visible within axon bundles and pronounced along the edge of axons bundles and at the pia surface. DAPI (blue) identifies cell nuclei. Scale Bar: 200 μm (A, D), 50 μm (B, C, E).

    Journal: PLoS ONE

    Article Title: Aquaporin 4 is not present in normal porcine and human lamina cribrosa

    doi: 10.1371/journal.pone.0268541

    Figure Lengend Snippet: Longitudinal cryosections of immunolabeled aquaporin-4 and phalloidin on human (A-E), and porcine (B) optic nerve head tissue. Minimal label for AQP4 (green) is visible in the lamina cribrosa (between dotted white lines in A, B; seen at higher power in C and E. AQP4 label is prominent in the retinal nerve fiber layer, prelaminar area and myelinated optic nerve in each species. Phalloidin labeling of F-actin (red) is prominent in the prelamina, lamina and myelinated ON (D, E). AQP4 labeling is visible within axon bundles and pronounced along the edge of axons bundles and at the pia surface. DAPI (blue) identifies cell nuclei. Scale Bar: 200 μm (A, D), 50 μm (B, C, E).

    Article Snippet: The transferred membrane was blocked for 1 h at room temperature with TBS buffer containing 5% non-fat dry milk followed by incubation with rabbit anti-AQP4 primary antibody (1:1000, Alamone labs) overnight on a shaker at 4°C.

    Techniques: Immunolabeling, Labeling

    Longitudinal (A-C) and cross-sections (D-I) of porcine optic nerve head labeled for aquaporin 4 (AQP4, green), myelin basic protein (MBP, red) and DAPI (blue). In longitudinal section, A, the lamina cribrosa is indicated by the zone between dotted white lines. Label for AQP4 is present in the retina and prelaminar region, absent in the lamina, and begins again coincident with the initial zone of MBP labeling. The lamina cribrosa in cross-section (D,E) is devoid of both AQP4 and MBP. In F and G, the section has lamina in the inferior area and the initial myelinated optic nerve present in the upper portion, showing that MBP staining begins just anterior to that of AQP4. The myelinated optic nerve (H, I) labels for both AQP4 and MBP. Scale Bar: 200 μm (A, D, E, F, G, H, I), 50 μm (B), 25 μm (C).

    Journal: PLoS ONE

    Article Title: Aquaporin 4 is not present in normal porcine and human lamina cribrosa

    doi: 10.1371/journal.pone.0268541

    Figure Lengend Snippet: Longitudinal (A-C) and cross-sections (D-I) of porcine optic nerve head labeled for aquaporin 4 (AQP4, green), myelin basic protein (MBP, red) and DAPI (blue). In longitudinal section, A, the lamina cribrosa is indicated by the zone between dotted white lines. Label for AQP4 is present in the retina and prelaminar region, absent in the lamina, and begins again coincident with the initial zone of MBP labeling. The lamina cribrosa in cross-section (D,E) is devoid of both AQP4 and MBP. In F and G, the section has lamina in the inferior area and the initial myelinated optic nerve present in the upper portion, showing that MBP staining begins just anterior to that of AQP4. The myelinated optic nerve (H, I) labels for both AQP4 and MBP. Scale Bar: 200 μm (A, D, E, F, G, H, I), 50 μm (B), 25 μm (C).

    Article Snippet: The transferred membrane was blocked for 1 h at room temperature with TBS buffer containing 5% non-fat dry milk followed by incubation with rabbit anti-AQP4 primary antibody (1:1000, Alamone labs) overnight on a shaker at 4°C.

    Techniques: Labeling, Staining

    Longitudinal (A, B) and cross-sections (C-F) of porcine tissue labeled for aquaporin 4 (AQP4, green), glial fibrillary acidic protein (GFAP, red) and DAPI (blue). Labeling for GFAP is visible throughout (A-C), in retina through the lamina and into the myelinated nerve, while AQP4 is only visible in the retina and myelinated nerve. Cross-sections of the lamina region (D, E) show label for GFAP but not for AQP4. Higher power cross-section of the transition zone from lamina to myelin shows co-incident labeling for GFAP and AQP4 in central lamina (F). In myelinated nerve, both labels are present, but are somewhat more distinct in position from each other (G). High power image (40x) shows the GFAP and AQP4 co-localization (yellow/orange) in astrocytic cells within and along the periphery of nerve bundles. Scale Bar: 150 μm (A, B), 50 μm (C, D, E), 25 μm (F, G) 10 μm (H).

    Journal: PLoS ONE

    Article Title: Aquaporin 4 is not present in normal porcine and human lamina cribrosa

    doi: 10.1371/journal.pone.0268541

    Figure Lengend Snippet: Longitudinal (A, B) and cross-sections (C-F) of porcine tissue labeled for aquaporin 4 (AQP4, green), glial fibrillary acidic protein (GFAP, red) and DAPI (blue). Labeling for GFAP is visible throughout (A-C), in retina through the lamina and into the myelinated nerve, while AQP4 is only visible in the retina and myelinated nerve. Cross-sections of the lamina region (D, E) show label for GFAP but not for AQP4. Higher power cross-section of the transition zone from lamina to myelin shows co-incident labeling for GFAP and AQP4 in central lamina (F). In myelinated nerve, both labels are present, but are somewhat more distinct in position from each other (G). High power image (40x) shows the GFAP and AQP4 co-localization (yellow/orange) in astrocytic cells within and along the periphery of nerve bundles. Scale Bar: 150 μm (A, B), 50 μm (C, D, E), 25 μm (F, G) 10 μm (H).

    Article Snippet: The transferred membrane was blocked for 1 h at room temperature with TBS buffer containing 5% non-fat dry milk followed by incubation with rabbit anti-AQP4 primary antibody (1:1000, Alamone labs) overnight on a shaker at 4°C.

    Techniques: Labeling

    Relative mRNA expression of AQP4 (A), GFAP (B), and CD68 (C) in regions of the porcine retina and optic nerve head. All samples were normalized to the geometric mean of the corresponding housekeeping gene values. R, Retina; L, Lamina; MTZ, myelin transition zone; MON, myelinated optic nerve. N = 4 replicates per region. Standard deviation error bars. Data was considered statistically significant if p

    Journal: PLoS ONE

    Article Title: Aquaporin 4 is not present in normal porcine and human lamina cribrosa

    doi: 10.1371/journal.pone.0268541

    Figure Lengend Snippet: Relative mRNA expression of AQP4 (A), GFAP (B), and CD68 (C) in regions of the porcine retina and optic nerve head. All samples were normalized to the geometric mean of the corresponding housekeeping gene values. R, Retina; L, Lamina; MTZ, myelin transition zone; MON, myelinated optic nerve. N = 4 replicates per region. Standard deviation error bars. Data was considered statistically significant if p

    Article Snippet: The transferred membrane was blocked for 1 h at room temperature with TBS buffer containing 5% non-fat dry milk followed by incubation with rabbit anti-AQP4 primary antibody (1:1000, Alamone labs) overnight on a shaker at 4°C.

    Techniques: Expressing, Standard Deviation, IF-P

    Western blot of aquaporin-4 (AQP4) on lamina and myelinate nerve (MON) of porcine tissue. Three biological replicates with three samples of lamina (lane 1,2,3) and three biological replicates with three samples of MON (lane 4,5,6) were processed. AQP4 was only detected in the MON samples, while none detected in the lamina tissues. All 6 lanes were positive for β-actin.

    Journal: PLoS ONE

    Article Title: Aquaporin 4 is not present in normal porcine and human lamina cribrosa

    doi: 10.1371/journal.pone.0268541

    Figure Lengend Snippet: Western blot of aquaporin-4 (AQP4) on lamina and myelinate nerve (MON) of porcine tissue. Three biological replicates with three samples of lamina (lane 1,2,3) and three biological replicates with three samples of MON (lane 4,5,6) were processed. AQP4 was only detected in the MON samples, while none detected in the lamina tissues. All 6 lanes were positive for β-actin.

    Article Snippet: The transferred membrane was blocked for 1 h at room temperature with TBS buffer containing 5% non-fat dry milk followed by incubation with rabbit anti-AQP4 primary antibody (1:1000, Alamone labs) overnight on a shaker at 4°C.

    Techniques: Western Blot