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Santa Cruz Biotechnology rabbit anti actin primary ab
Rabbit Anti Actin Primary Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti actin primary ab/product/Santa Cruz Biotechnology
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti actin primary ab - by Bioz Stars, 2020-09
85/100 stars

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Article Title: Potential Role of Activating Transcription Factor 5 during Osteogenesis
Article Snippet: Then, the protocol was repeated for quantification of actin, using a rabbit anti-actin primary antibody (Santa Cruz, Catalog number SC130657; dilution 1 : 500) followed by a goat anti-rabbit secondary antibody (Pierce Biotechnology Inc., Thermo Fisher Scientific, Waltham, MA, USA; Catalog number 1858413; dilution 1 : 5000).

Article Title: TGF-? Promotes Proliferation of Thyroid Epithelial Cells in IFN-?−/− Mice by Down-Regulation of p21 and p27 via AKT Pathway
Article Snippet: For normalization of signals, the membranes were stripped and reprobed with 1/2000 rabbit anti-actin primary antibody (Santa Cruz Biotechnology) and 1/3000 HRP-conjugated anti-rabbit IgG as secondary antibody, as described previously.

Article Title: Agonistic anti-CD40 induces thyrocyte proliferation and promotes thyroid autoimmunity by increasing CD40 expression on thyroid epithelial cells 1
Article Snippet: For normalization, membranes were stripped and reprobed with rabbit anti-actin primary antibody (sc 47778; Santa Cruz) and 1/3000 Horse Radish Peroxidase (HRP)-conjugated anti-rabbit IgG (Jackson ImmunoResearch) as described previously ( ).

Stripping Membranes:

Article Title: Murine FLIP Transgene Expressed on Thyroid Epithelial Cells Promotes Resolution of Granulomatous Experimental Autoimmune Thyroiditis in DBA/1 Mice
Article Snippet: .. For normalization of signals, the membranes were stripped by incubating at 60°C for 30 minutes in stripping buffer (62.5 mmol/L Tris-HCl, 100 mmol/L 2-ME, and 2% sodium dodecyl sulfate), washed in Tris-buffered saline/Tween 20, and reprobed with 1/2000 rabbit anti-actin primary Ab (Santa Cruz Biotechnology) and 1/2000 horseradish peroxidase-conjugated anti-rabbit IgG as secondary Ab. .. Bands were scanned and quantitated using Quality One-4.41 software.

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    Santa Cruz Biotechnology rabbit anti human β actin
    Effect of cytokines on the expression of NOD2 in first trimester DSCs. The primary-cultured DSCs from normal pregnant women were starved with 1% FBS for 12 h and then treated with vehicle, IL-1β, TNF-α, IFN-γ or IL-17A of different concentration (1 ng/ml, 10 ng/ml, 100 ng/ml) for 24 hrs respectively. The mRNA and protein level of NOD2 was determined by real-time PCR and In-cell Western. The pictures were from one representative of three individual experiments. (A) IL-1β increased the NOD2 mRNA expression in DSCs. (B) IFN-γ or IL-17A, but did not affect NOD2 expression after 24 hrs stimulation. (C) NOD2 was elevated in DSCs after the stimulation with TNF-α and IL1β. The bars indicate the ratio of the intensity of NOD2 protein to that of <t>β-actin.</t> Error bars depict the standard error of the mean. Data represent mean of fold change ±SEM. * P
    Rabbit Anti Human β Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human β actin/product/Santa Cruz Biotechnology
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human β actin - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology primary antibodies rabbit anti β actin
    Time course regulation of cell death–related proteins in naïve and optic nerve injured retinas. Western blot time course analyses showing the regulation of tumor necrosis factor receptor superfamily member 1a (TNFR1a), tumor necrosis factor receptor type 1, associated death domain (TRADD), total Caspase 3, active Caspase 3, and c-fos in naïve, intraorbital nerve transection (IONT)-, and intraorbital nerve crush (IONC)-injured retinas. Graphs show quantification of protein signals (n=4 animals per lesion and time point, western blots were replicated three times). The signal from injured retinas is referred to the naïve signal, which was arbitrarily considered 100%. To verify the amount of loaded protein, western blots were incubated with <t>β-actin</t> (an example is shown). Error bars show the standard error of the mean (SEM) for each experiment.
    Primary Antibodies Rabbit Anti β Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies rabbit anti β actin/product/Santa Cruz Biotechnology
    Average 85 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    primary antibodies rabbit anti β actin - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology rabbit anti mouse β actin primary antibody
    Effect of antioxidant intervention NAC on IH-induced Bcl-2 and Bax expression (western blotting). ( a ) A representation of western blotting analysis suggested antioxidant (NAC) intervention reversed IH-induced Bcl-2 and Bax expression. <t>β-Actin</t> was used as for normalization. ( b ) The statistical analyses of the Bcl-2 and Bax expression in the pancreatic β-cells of the four groups. These results are expressed as x̄ ± s ( n =6 per group, ‘▴,★' indicate significance compared with the intermittent normoxia group, P
    Rabbit Anti Mouse β Actin Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse β actin primary antibody/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse β actin primary antibody - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effect of cytokines on the expression of NOD2 in first trimester DSCs. The primary-cultured DSCs from normal pregnant women were starved with 1% FBS for 12 h and then treated with vehicle, IL-1β, TNF-α, IFN-γ or IL-17A of different concentration (1 ng/ml, 10 ng/ml, 100 ng/ml) for 24 hrs respectively. The mRNA and protein level of NOD2 was determined by real-time PCR and In-cell Western. The pictures were from one representative of three individual experiments. (A) IL-1β increased the NOD2 mRNA expression in DSCs. (B) IFN-γ or IL-17A, but did not affect NOD2 expression after 24 hrs stimulation. (C) NOD2 was elevated in DSCs after the stimulation with TNF-α and IL1β. The bars indicate the ratio of the intensity of NOD2 protein to that of β-actin. Error bars depict the standard error of the mean. Data represent mean of fold change ±SEM. * P

    Journal: PLoS ONE

    Article Title: Expression and Functional Characterization of NOD2 in Decidual Stromal Cells Isolated during the First Trimester of Pregnancy

    doi: 10.1371/journal.pone.0099612

    Figure Lengend Snippet: Effect of cytokines on the expression of NOD2 in first trimester DSCs. The primary-cultured DSCs from normal pregnant women were starved with 1% FBS for 12 h and then treated with vehicle, IL-1β, TNF-α, IFN-γ or IL-17A of different concentration (1 ng/ml, 10 ng/ml, 100 ng/ml) for 24 hrs respectively. The mRNA and protein level of NOD2 was determined by real-time PCR and In-cell Western. The pictures were from one representative of three individual experiments. (A) IL-1β increased the NOD2 mRNA expression in DSCs. (B) IFN-γ or IL-17A, but did not affect NOD2 expression after 24 hrs stimulation. (C) NOD2 was elevated in DSCs after the stimulation with TNF-α and IL1β. The bars indicate the ratio of the intensity of NOD2 protein to that of β-actin. Error bars depict the standard error of the mean. Data represent mean of fold change ±SEM. * P

    Article Snippet: Cells were incubated overnight at 4°C with mouse anti-human mAb NOD2 (25 µg/ml, Abcam, Cambridge, UK) primary antibody and the rabbit anti-human β-actin (1∶80, Santa Cruz, CA, USA) which detected the control reference protein.

    Techniques: Expressing, Cell Culture, Concentration Assay, Real-time Polymerase Chain Reaction, In-Cell ELISA

    Time course regulation of cell death–related proteins in naïve and optic nerve injured retinas. Western blot time course analyses showing the regulation of tumor necrosis factor receptor superfamily member 1a (TNFR1a), tumor necrosis factor receptor type 1, associated death domain (TRADD), total Caspase 3, active Caspase 3, and c-fos in naïve, intraorbital nerve transection (IONT)-, and intraorbital nerve crush (IONC)-injured retinas. Graphs show quantification of protein signals (n=4 animals per lesion and time point, western blots were replicated three times). The signal from injured retinas is referred to the naïve signal, which was arbitrarily considered 100%. To verify the amount of loaded protein, western blots were incubated with β-actin (an example is shown). Error bars show the standard error of the mean (SEM) for each experiment.

    Journal: Molecular Vision

    Article Title: Time course profiling of the retinal transcriptome after optic nerve transection and optic nerve crush

    doi:

    Figure Lengend Snippet: Time course regulation of cell death–related proteins in naïve and optic nerve injured retinas. Western blot time course analyses showing the regulation of tumor necrosis factor receptor superfamily member 1a (TNFR1a), tumor necrosis factor receptor type 1, associated death domain (TRADD), total Caspase 3, active Caspase 3, and c-fos in naïve, intraorbital nerve transection (IONT)-, and intraorbital nerve crush (IONC)-injured retinas. Graphs show quantification of protein signals (n=4 animals per lesion and time point, western blots were replicated three times). The signal from injured retinas is referred to the naïve signal, which was arbitrarily considered 100%. To verify the amount of loaded protein, western blots were incubated with β-actin (an example is shown). Error bars show the standard error of the mean (SEM) for each experiment.

    Article Snippet: Primary antibodies Rabbit anti-β-actin (Santa Cruz Biotechnologies, Heidelberg, Germany) was diluted at 1:200 for western blotting analysis.

    Techniques: Western Blot, Incubation

    A) The ATP content of the mouse blastocysts was detected via firefly luciferase analysis. B) The relative expression levels of mtDNA and cDNA. Values are presented as multiples of COXII DNA relative to the β-actin DNA. C) Mitochondrial membrane potential was assessed by JC-1. D) Statistical analyses of the red and green mean fluorescence intensity ratios revealed that the mitochondrial membrane potential of the T group was significantly reduced compared with the C group, * P

    Journal: PLoS ONE

    Article Title: Mfn2 Affects Embryo Development via Mitochondrial Dysfunction and Apoptosis

    doi: 10.1371/journal.pone.0125680

    Figure Lengend Snippet: A) The ATP content of the mouse blastocysts was detected via firefly luciferase analysis. B) The relative expression levels of mtDNA and cDNA. Values are presented as multiples of COXII DNA relative to the β-actin DNA. C) Mitochondrial membrane potential was assessed by JC-1. D) Statistical analyses of the red and green mean fluorescence intensity ratios revealed that the mitochondrial membrane potential of the T group was significantly reduced compared with the C group, * P

    Article Snippet: The membranes were hybridized with rabbit anti-Mfn2 (Abcam, Cambridge, MA), rabbit anti-Bax, rabbit anti-Bcl-2, and rabbit anti-β-actin primary antibodies (Santa Cruz Biotech, CA) in Tris-buffered saline with Tween (TBS-T) containing 1% nonfat milk at 4°C overnight.

    Techniques: Luciferase, Expressing, Fluorescence

    Effect of antioxidant intervention NAC on IH-induced Bcl-2 and Bax expression (western blotting). ( a ) A representation of western blotting analysis suggested antioxidant (NAC) intervention reversed IH-induced Bcl-2 and Bax expression. β-Actin was used as for normalization. ( b ) The statistical analyses of the Bcl-2 and Bax expression in the pancreatic β-cells of the four groups. These results are expressed as x̄ ± s ( n =6 per group, ‘▴,★' indicate significance compared with the intermittent normoxia group, P

    Journal: Nutrition & Diabetes

    Article Title: Intermittent hypoxia-induced rat pancreatic β-cell apoptosis and protective effects of antioxidant intervention

    doi: 10.1038/nutd.2014.28

    Figure Lengend Snippet: Effect of antioxidant intervention NAC on IH-induced Bcl-2 and Bax expression (western blotting). ( a ) A representation of western blotting analysis suggested antioxidant (NAC) intervention reversed IH-induced Bcl-2 and Bax expression. β-Actin was used as for normalization. ( b ) The statistical analyses of the Bcl-2 and Bax expression in the pancreatic β-cells of the four groups. These results are expressed as x̄ ± s ( n =6 per group, ‘▴,★' indicate significance compared with the intermittent normoxia group, P

    Article Snippet: Materials and methods Terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling (TUNEL) apoptosis detection kit (Roche, Indianapolis, IN, USA), rabbit anti-Bcl-2 antibody, rabbit anti-Bax antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit anti-mouse β-actin primary antibody (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (Boaosen, Beijing, China).

    Techniques: Expressing, Western Blot