rabbit anti phospho 14 3 3 binding motif pab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti phospho 14 3 3 binding motif pab
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
    Rabbit Anti Phospho 14 3 3 Binding Motif Pab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The 14-3-3γ isoform binds to and regulates the localization of endoplasmic reticulum (ER) membrane protein TMCC3 for the reticular network of the ER"

    Article Title: The 14-3-3γ isoform binds to and regulates the localization of endoplasmic reticulum (ER) membrane protein TMCC3 for the reticular network of the ER

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2022.102813

    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the anti-phospho-14-3-3 binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
    Figure Legend Snippet: Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the anti-phospho-14-3-3 binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.

    Techniques Used: Binding Assay, Transfection, Immunoprecipitation

    rabbit anti 14 3 3 binding motif  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti 14 3 3 binding motif
    Rabbit Anti 14 3 3 Binding Motif, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti rat phospho ser473akt monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti rat phospho ser473akt monoclonal antibody
    Rabbit Anti Rat Phospho Ser473akt Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal rabbit phospho  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal rabbit phospho
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    rabbit polyclonal anti phospho ser780rb antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti phospho ser780rb antibody
    Rabbit Polyclonal Anti Phospho Ser780rb Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti phospho ser1177enos antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti phospho ser1177enos antibody
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    rabbit polyclonal  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal
    List of antibodies used
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    1) Product Images from "Interferon Tau Regulates PGF2α Release from the Ovine Endometrial Epithelial Cells via Activation of Novel JAK/EGFR/ERK/EGR-1 Pathways"

    Article Title: Interferon Tau Regulates PGF2α Release from the Ovine Endometrial Epithelial Cells via Activation of Novel JAK/EGFR/ERK/EGR-1 Pathways

    Journal: Molecular Endocrinology

    doi: 10.1210/me.2010-0205

    List of antibodies used
    Figure Legend Snippet: List of antibodies used

    Techniques Used: Concentration Assay

    rabbit anti phospho ser133creb  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti phospho ser133creb
    Rabbit Anti Phospho Ser133creb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti phospho ser171 torc2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti phospho ser171 torc2
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    rabbit polyclonal anti phospho ser171 torc2  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit polyclonal anti phospho ser171 torc2
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    Cell Signaling Technology Inc rabbit anti phospho 14 3 3 binding motif pab
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
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    Cell Signaling Technology Inc rabbit anti 14 3 3 binding motif
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
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    Cell Signaling Technology Inc rabbit anti rat phospho ser473akt monoclonal antibody
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
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    Cell Signaling Technology Inc polyclonal rabbit phospho
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
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    Cell Signaling Technology Inc rabbit polyclonal anti phospho ser780rb antibody
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
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    Cell Signaling Technology Inc rabbit anti phospho ser1177enos antibody
    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the <t>anti-phospho-14-3-3</t> binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.
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    Cell Signaling Technology Inc rabbit polyclonal
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    Cell Signaling Technology Inc rabbit anti phospho ser133creb
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    Cell Signaling Technology Inc rabbit polyclonal anti phospho ser171 torc2
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    Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the anti-phospho-14-3-3 binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.

    Journal: The Journal of Biological Chemistry

    Article Title: The 14-3-3γ isoform binds to and regulates the localization of endoplasmic reticulum (ER) membrane protein TMCC3 for the reticular network of the ER

    doi: 10.1016/j.jbc.2022.102813

    Figure Lengend Snippet: Phosphorylated serine 15 in the deduced 14-3-3 binding motif is required for potent binding to 14-3-3γ . A , the N-terminal region of TMCC3 highlighting the deduced 14-3-3 binding motifs. Serine residues being the possible phosphorylation sites in three deduced 14-3-3 binding motifs are indicated in red . Arginine, serine, and proline residues at −3, 0, and +2 positions, respectively, in the deduced 14-3-3 binding motif predicted with the highest score are underlined. B , serine 15 is required for potent binding to 14-3-3γ. Indicated combinations of HA-TMCC3-D1, HA-TMCC3-D1-S15A, HA-TMCC3-D1-S15/25/46A, and Flag-14-3-3γ were transfected into HEK293 cells, followed by immunoprecipitation with the anti-Flag mAb. The samples were immunoblotted with the anti-HA mAb and the anti-Flag pAb. The arrowheads indicate the bands of interest. The asterisk s indicate the nonspecific bands from the light chain of IgG. C , serine 15 is phosphorylated. HA-TMCC3-D1, HA-TMCC3-D1-S15A, and HA-TMCC3-D1-S15/25/46A were transfected into HEK293 cells, followed by immunoprecipitation with the anti-HA mAb. The samples were immunoblotted with the anti-phospho-14-3-3 binding motif pAb and the anti-HA pAb. The asterisk indicates the nonspecific bands from the light chain of IgG. TMCC3, transmembrane and coiled-coil domain family 3.

    Article Snippet: The antibodies were purchased from the following commercial sources: mouse anti-HA mAb (BioLegend; Cat. No. 901514), rabbit anti-HA pAb (Sigma-Aldrich; Cat. No. H6908), rat anti-HA mAb (Roche; Cat. No. 11867423001), mouse anti-FLAG mAb (Sigma-Aldrich; Cat. No. F1804), rabbit anti-FLAG pAb (Sigma-Aldrich; Cat. No. F7425), rabbit anti-TMCC3 pAb (Sigma-Aldrich; Cat. No. HPA014272), rabbit anti-CLIMP-63 pAb (Bethyl Laboratories; Cat. No. A302–257A), mouse anti-α-tubulin mAb (Sigma-Aldrich; Cat. No. T6199), mouse anti-14-3-3 mAb (Santa Cruz Biotechnology; Cat. No. sc-1657), rabbit anti-phospho 14-3-3 binding motif pAb (Cell Signaling; Cat. No. 9601), mouse anti-PDI mAb (Abcam; Cat. No. ab2792), rat anti-GFP mAb (Nacalai; Cat. No. 04404–84), rabbit anti-GFP pAb (MBL; Cat. No. 598), and mouse anti-GAPDH mAb-HRP-DirecT (MBL; Cat. No. M171–7).

    Techniques: Binding Assay, Transfection, Immunoprecipitation

    List of antibodies used

    Journal: Molecular Endocrinology

    Article Title: Interferon Tau Regulates PGF2α Release from the Ovine Endometrial Epithelial Cells via Activation of Novel JAK/EGFR/ERK/EGR-1 Pathways

    doi: 10.1210/me.2010-0205

    Figure Lengend Snippet: List of antibodies used

    Article Snippet: 26 , p-Serine , Rabbit polyclonal , Ser , 1:1000 , 9601 , Cell Signaling.

    Techniques: Concentration Assay