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Vector Biolabs raav gfp control vector
VGF mRNA and eGFP expression patterns are similar following <t>rAAV-VGF</t> infusio n into the hypothalamus of both Siberian hamsters and mice . Representative pictures of eGFP expression (left) and VGF mRNA expression via in situ hybridisation (right) in the hypothalamus of Siberian hamsters (A and B) and mice (C and D) receiving bilateral injections of either <t>rAAV-GFP</t> (A and C) or rAAV-VGF (B and D) directed to the PVN. Scale bar = 2.5 mm.
Raav Gfp Control Vector, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/raav gfp control vector/product/Vector Biolabs
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
raav gfp control vector - by Bioz Stars, 2020-08
88/100 stars

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1) Product Images from "The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo"

Article Title: The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo

Journal: Journal of Neuroscience Methods

doi: 10.1016/j.jneumeth.2015.08.013

VGF mRNA and eGFP expression patterns are similar following rAAV-VGF infusio n into the hypothalamus of both Siberian hamsters and mice . Representative pictures of eGFP expression (left) and VGF mRNA expression via in situ hybridisation (right) in the hypothalamus of Siberian hamsters (A and B) and mice (C and D) receiving bilateral injections of either rAAV-GFP (A and C) or rAAV-VGF (B and D) directed to the PVN. Scale bar = 2.5 mm.
Figure Legend Snippet: VGF mRNA and eGFP expression patterns are similar following rAAV-VGF infusio n into the hypothalamus of both Siberian hamsters and mice . Representative pictures of eGFP expression (left) and VGF mRNA expression via in situ hybridisation (right) in the hypothalamus of Siberian hamsters (A and B) and mice (C and D) receiving bilateral injections of either rAAV-GFP (A and C) or rAAV-VGF (B and D) directed to the PVN. Scale bar = 2.5 mm.

Techniques Used: Expressing, Mouse Assay, In Situ, Hybridization

A schematic to show steps leading to the production of rAAV-VGF . Full length mouse cDNA was isolated and inserted into the blunt cloning vector pSC-B-AMP/KAN for amplification using modified primers to produce the VGF-2A fragment. This product was cloned into the pAAV-CBA-AgRP-IRES-GFP backbone (previously used in Jethwa et al., 2010 ), following excision of the AgRP-IRES complex to produce pAAV-CBA-VGF-2a-GFP (pAAV-VGF-GFP). This was used for the in vitro checking (Study 1), before then being used to produce recombinant AAV-2 (rAAV) by Vector BioLabs (USA) yielding rAAV-VGF for in vivo infusion (study 2). ITR—internal tandem repeat, CMV/IE—cytomegalovirus intermediate early promoter, CBA—chicken β-actin promoter, eGFP—enhanced green fluorescent protein, WPRE—Woodchuck Hepatitis virus post-transcriptional regulatory element.
Figure Legend Snippet: A schematic to show steps leading to the production of rAAV-VGF . Full length mouse cDNA was isolated and inserted into the blunt cloning vector pSC-B-AMP/KAN for amplification using modified primers to produce the VGF-2A fragment. This product was cloned into the pAAV-CBA-AgRP-IRES-GFP backbone (previously used in Jethwa et al., 2010 ), following excision of the AgRP-IRES complex to produce pAAV-CBA-VGF-2a-GFP (pAAV-VGF-GFP). This was used for the in vitro checking (Study 1), before then being used to produce recombinant AAV-2 (rAAV) by Vector BioLabs (USA) yielding rAAV-VGF for in vivo infusion (study 2). ITR—internal tandem repeat, CMV/IE—cytomegalovirus intermediate early promoter, CBA—chicken β-actin promoter, eGFP—enhanced green fluorescent protein, WPRE—Woodchuck Hepatitis virus post-transcriptional regulatory element.

Techniques Used: Isolation, Clone Assay, Plasmid Preparation, Amplification, Modification, Crocin Bleaching Assay, In Vitro, Recombinant, In Vivo

Use of the viral 2A sequence in the rAAV-VGF construct results in widespread eGFP expression in the hypothalamus . Representative pictures of eGFP expression in the hypothalamus of Siberian hamsters and mice which received bilateral (200 nl) injections of either rAAV-GFP or rAAV-VGF directed to the PVN. (A–D) eGFP expression following infusion of rAAV-GFP control vector in the whole hypothalamus of (A) Siberian hamsters and (B) mice and in the cell soma and processes of the PVN of (C) Siberian hamsters and (D) mice. (E–G) eGFP expression following infusion of the rAAV-VGF vector in the hypothalamus of (E) Siberian hamsters and (F) mice and in the cell soma and processes of the PVN of (G) Siberian hamsters and (H) mice. Scale bar = 2.5 mm.
Figure Legend Snippet: Use of the viral 2A sequence in the rAAV-VGF construct results in widespread eGFP expression in the hypothalamus . Representative pictures of eGFP expression in the hypothalamus of Siberian hamsters and mice which received bilateral (200 nl) injections of either rAAV-GFP or rAAV-VGF directed to the PVN. (A–D) eGFP expression following infusion of rAAV-GFP control vector in the whole hypothalamus of (A) Siberian hamsters and (B) mice and in the cell soma and processes of the PVN of (C) Siberian hamsters and (D) mice. (E–G) eGFP expression following infusion of the rAAV-VGF vector in the hypothalamus of (E) Siberian hamsters and (F) mice and in the cell soma and processes of the PVN of (G) Siberian hamsters and (H) mice. Scale bar = 2.5 mm.

Techniques Used: Sequencing, Construct, Expressing, Mouse Assay, Plasmid Preparation

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Plasmid Preparation:

Article Title: The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo
Article Snippet: .. The rAAV-GFP control vector was purchased from Vector Biolabs (PA, USA); this had a viral titre of 1 × 1013 gc/ml and expressed eGFP under the control of a cytomegalovirus (CMV) promoter. .. 2.2 The SH-SY5Y neuroblastoma cell line The human neuroblastoma SH-SY5Y cells were grown in 25 cm2 tissue culture flasks with complete Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham, containing 10% foetal bovine serum, 100 units/l penicillin and 100 mg/l streptomycin (all Sigma-Aldrich, Dorset, UK), which will be referred to as DMEM/F12 complete.

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    Vector Biolabs raav gfp control vector
    VGF mRNA and eGFP expression patterns are similar following <t>rAAV-VGF</t> infusio n into the hypothalamus of both Siberian hamsters and mice . Representative pictures of eGFP expression (left) and VGF mRNA expression via in situ hybridisation (right) in the hypothalamus of Siberian hamsters (A and B) and mice (C and D) receiving bilateral injections of either <t>rAAV-GFP</t> (A and C) or rAAV-VGF (B and D) directed to the PVN. Scale bar = 2.5 mm.
    Raav Gfp Control Vector, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raav gfp control vector/product/Vector Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    raav gfp control vector - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

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    VGF mRNA and eGFP expression patterns are similar following rAAV-VGF infusio n into the hypothalamus of both Siberian hamsters and mice . Representative pictures of eGFP expression (left) and VGF mRNA expression via in situ hybridisation (right) in the hypothalamus of Siberian hamsters (A and B) and mice (C and D) receiving bilateral injections of either rAAV-GFP (A and C) or rAAV-VGF (B and D) directed to the PVN. Scale bar = 2.5 mm.

    Journal: Journal of Neuroscience Methods

    Article Title: The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo

    doi: 10.1016/j.jneumeth.2015.08.013

    Figure Lengend Snippet: VGF mRNA and eGFP expression patterns are similar following rAAV-VGF infusio n into the hypothalamus of both Siberian hamsters and mice . Representative pictures of eGFP expression (left) and VGF mRNA expression via in situ hybridisation (right) in the hypothalamus of Siberian hamsters (A and B) and mice (C and D) receiving bilateral injections of either rAAV-GFP (A and C) or rAAV-VGF (B and D) directed to the PVN. Scale bar = 2.5 mm.

    Article Snippet: The rAAV-GFP control vector was purchased from Vector Biolabs (PA, USA); this had a viral titre of 1 × 1013 gc/ml and expressed eGFP under the control of a cytomegalovirus (CMV) promoter.

    Techniques: Expressing, Mouse Assay, In Situ, Hybridization

    A schematic to show steps leading to the production of rAAV-VGF . Full length mouse cDNA was isolated and inserted into the blunt cloning vector pSC-B-AMP/KAN for amplification using modified primers to produce the VGF-2A fragment. This product was cloned into the pAAV-CBA-AgRP-IRES-GFP backbone (previously used in Jethwa et al., 2010 ), following excision of the AgRP-IRES complex to produce pAAV-CBA-VGF-2a-GFP (pAAV-VGF-GFP). This was used for the in vitro checking (Study 1), before then being used to produce recombinant AAV-2 (rAAV) by Vector BioLabs (USA) yielding rAAV-VGF for in vivo infusion (study 2). ITR—internal tandem repeat, CMV/IE—cytomegalovirus intermediate early promoter, CBA—chicken β-actin promoter, eGFP—enhanced green fluorescent protein, WPRE—Woodchuck Hepatitis virus post-transcriptional regulatory element.

    Journal: Journal of Neuroscience Methods

    Article Title: The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo

    doi: 10.1016/j.jneumeth.2015.08.013

    Figure Lengend Snippet: A schematic to show steps leading to the production of rAAV-VGF . Full length mouse cDNA was isolated and inserted into the blunt cloning vector pSC-B-AMP/KAN for amplification using modified primers to produce the VGF-2A fragment. This product was cloned into the pAAV-CBA-AgRP-IRES-GFP backbone (previously used in Jethwa et al., 2010 ), following excision of the AgRP-IRES complex to produce pAAV-CBA-VGF-2a-GFP (pAAV-VGF-GFP). This was used for the in vitro checking (Study 1), before then being used to produce recombinant AAV-2 (rAAV) by Vector BioLabs (USA) yielding rAAV-VGF for in vivo infusion (study 2). ITR—internal tandem repeat, CMV/IE—cytomegalovirus intermediate early promoter, CBA—chicken β-actin promoter, eGFP—enhanced green fluorescent protein, WPRE—Woodchuck Hepatitis virus post-transcriptional regulatory element.

    Article Snippet: The rAAV-GFP control vector was purchased from Vector Biolabs (PA, USA); this had a viral titre of 1 × 1013 gc/ml and expressed eGFP under the control of a cytomegalovirus (CMV) promoter.

    Techniques: Isolation, Clone Assay, Plasmid Preparation, Amplification, Modification, Crocin Bleaching Assay, In Vitro, Recombinant, In Vivo

    Use of the viral 2A sequence in the rAAV-VGF construct results in widespread eGFP expression in the hypothalamus . Representative pictures of eGFP expression in the hypothalamus of Siberian hamsters and mice which received bilateral (200 nl) injections of either rAAV-GFP or rAAV-VGF directed to the PVN. (A–D) eGFP expression following infusion of rAAV-GFP control vector in the whole hypothalamus of (A) Siberian hamsters and (B) mice and in the cell soma and processes of the PVN of (C) Siberian hamsters and (D) mice. (E–G) eGFP expression following infusion of the rAAV-VGF vector in the hypothalamus of (E) Siberian hamsters and (F) mice and in the cell soma and processes of the PVN of (G) Siberian hamsters and (H) mice. Scale bar = 2.5 mm.

    Journal: Journal of Neuroscience Methods

    Article Title: The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo

    doi: 10.1016/j.jneumeth.2015.08.013

    Figure Lengend Snippet: Use of the viral 2A sequence in the rAAV-VGF construct results in widespread eGFP expression in the hypothalamus . Representative pictures of eGFP expression in the hypothalamus of Siberian hamsters and mice which received bilateral (200 nl) injections of either rAAV-GFP or rAAV-VGF directed to the PVN. (A–D) eGFP expression following infusion of rAAV-GFP control vector in the whole hypothalamus of (A) Siberian hamsters and (B) mice and in the cell soma and processes of the PVN of (C) Siberian hamsters and (D) mice. (E–G) eGFP expression following infusion of the rAAV-VGF vector in the hypothalamus of (E) Siberian hamsters and (F) mice and in the cell soma and processes of the PVN of (G) Siberian hamsters and (H) mice. Scale bar = 2.5 mm.

    Article Snippet: The rAAV-GFP control vector was purchased from Vector Biolabs (PA, USA); this had a viral titre of 1 × 1013 gc/ml and expressed eGFP under the control of a cytomegalovirus (CMV) promoter.

    Techniques: Sequencing, Construct, Expressing, Mouse Assay, Plasmid Preparation