Structured Review

InvivoGen r837
TLR7 agonist <t>R837</t> rescued mice from paralysis and death efficiently after viral infection. Upper panel: A schematic diagram of R837 treatment via an oral or i.p. route after EV-A71 infection. Lower panel: Mice were treated with or without R837 (5.0 μg /g of mouse) at dpi = 1. Significant reduction in clinical score was observed only in the two groups treated with R837. *** p
R837, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 12 article reviews
Price from $9.99 to $1999.99
r837 - by Bioz Stars, 2020-01
99/100 stars

Images

1) Product Images from "Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model"

Article Title: Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model

Journal: Journal of Biomedical Science

doi: 10.1186/s12929-019-0585-y

TLR7 agonist R837 rescued mice from paralysis and death efficiently after viral infection. Upper panel: A schematic diagram of R837 treatment via an oral or i.p. route after EV-A71 infection. Lower panel: Mice were treated with or without R837 (5.0 μg /g of mouse) at dpi = 1. Significant reduction in clinical score was observed only in the two groups treated with R837. *** p
Figure Legend Snippet: TLR7 agonist R837 rescued mice from paralysis and death efficiently after viral infection. Upper panel: A schematic diagram of R837 treatment via an oral or i.p. route after EV-A71 infection. Lower panel: Mice were treated with or without R837 (5.0 μg /g of mouse) at dpi = 1. Significant reduction in clinical score was observed only in the two groups treated with R837. *** p

Techniques Used: Mouse Assay, Infection

2) Product Images from "HIV-1 Activates Macrophages Independent of Toll-Like Receptors"

Article Title: HIV-1 Activates Macrophages Independent of Toll-Like Receptors

Journal: PLoS ONE

doi: 10.1371/journal.pone.0003664

HIV-1 priming enhances macrophage cytokine production in response to TLR stimulation. Macrophages were treated with HIV-1 or media alone for 24 hours. Virus was allowed to spread in culture for an additional 9 days. Macrophages in the absence (gray bars) or presence (black bars) of spreading virus were treated with media, HIV-1, LPS, Poly(I:C), CL097, CL075, or R837 for 24 hours, and supernatant TNF (A), IL-6 (B), or IL-1β (C) levels were measured.
Figure Legend Snippet: HIV-1 priming enhances macrophage cytokine production in response to TLR stimulation. Macrophages were treated with HIV-1 or media alone for 24 hours. Virus was allowed to spread in culture for an additional 9 days. Macrophages in the absence (gray bars) or presence (black bars) of spreading virus were treated with media, HIV-1, LPS, Poly(I:C), CL097, CL075, or R837 for 24 hours, and supernatant TNF (A), IL-6 (B), or IL-1β (C) levels were measured.

Techniques Used:

Related Articles

Flow Cytometry:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: An average of 1 × 104 LCs per cm2 of tissue with a purity higher than 90% was obtained and characterized as CD1a+ langerin+ cells by flow cytometry as described below. .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation.

Generated:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: After incubation, a single-cell suspension was generated using 100 μm nylon cell strainers (BD Falcon, Corning, NY, USA), and cells were layered onto a Ficoll gradient. .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation.

Cytometry:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: An average of 1 × 104 LCs per cm2 of tissue with a purity higher than 90% was obtained and characterized as CD1a+ langerin+ cells by flow cytometry as described below. .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation.

Cell Culture:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation. .. In the indicated experiments, LCs were obtained by spontaneous migration.

Mouse Assay:

Article Title: Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model
Article Snippet: .. Recombinant mouse IFNαA (PBL Assay Science Co.), GS9620 (Cayman Chemical) and R837 (InvivoGen), were orally or i.p. injected into wt-129 mice infected with EV-A71, at the dose of 300 IU/g (mouse IFNαA), 3.0 μg/g (GS-9620), 4.5μg/g (GS-9620), and 5.0 μg/g (R837), respectively. .. Generation of IL28b KO mouse model Two IFN-lambda knockout (KO) mouse lineages were established in this study.

Enzyme-linked Immunosorbent Assay:

Article Title: HIV-1 Activates Macrophages Independent of Toll-Like Receptors
Article Snippet: Macrophages were washed with PBS and treated for 24 hours with 1 µg/ml E.coli 0111:B4 LPS (Sigma-Aldrich), 100 µg/ml Poly(I:C) (Invivogen), 1 µg/ml CL097 (Invivogen), 1 µg/ml CL075 (Invivogen), 10 µg/ml R837 (Invivogen), 5,000 TCID50 HIV-1AD , or media. .. Supernatants were collected and levels of TNF, IL-6, and IL-1β were quantified by ELISA (BD Biosciences).

Plasmid Purification:

Article Title: Vitamin B6 Prevents IL-1β Protein Production by Inhibiting NLRP3 Inflammasome Activation *
Article Snippet: .. PL hydrochloride, PN hydrochloride, PLP (Nacalai Tesque, Kyoto, Japan), PM dihydrochloride (Calbiochem), LPS from Escherichia coli K235 and from E. coli 0111:B4, ATP, nigericin, poly(I:C) (Sigma-Aldrich), Pam3CSK4, R837 (InvivoGen, San Diego, CA), CpG oligodeoxynucleotides (Genset Oligos, La Jolla, CA), and MSU (Wako, Osaka Japan) were purchased. .. C57BL/6J mice aged 8–12 weeks were injected i.p. with 3% thioglycollate solution, and peritoneal exudate cells were collected 4 days later.

Incubation:

Article Title: HIV-1 Activates Macrophages Independent of Toll-Like Receptors
Article Snippet: Cells were washed with PBS and incubated in media for 9 days with 50% media changes every 2 days. .. Macrophages were washed with PBS and treated for 24 hours with 1 µg/ml E.coli 0111:B4 LPS (Sigma-Aldrich), 100 µg/ml Poly(I:C) (Invivogen), 1 µg/ml CL097 (Invivogen), 1 µg/ml CL075 (Invivogen), 10 µg/ml R837 (Invivogen), 5,000 TCID50 HIV-1AD , or media.

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: After incubation, a single-cell suspension was generated using 100 μm nylon cell strainers (BD Falcon, Corning, NY, USA), and cells were layered onto a Ficoll gradient. .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation.

Infection:

Article Title: Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model
Article Snippet: .. Recombinant mouse IFNαA (PBL Assay Science Co.), GS9620 (Cayman Chemical) and R837 (InvivoGen), were orally or i.p. injected into wt-129 mice infected with EV-A71, at the dose of 300 IU/g (mouse IFNαA), 3.0 μg/g (GS-9620), 4.5μg/g (GS-9620), and 5.0 μg/g (R837), respectively. .. Generation of IL28b KO mouse model Two IFN-lambda knockout (KO) mouse lineages were established in this study.

Modification:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: The slices were incubated in dispase II (1 mg mL–1 , Roche Diagnostics, Basil, Switzerland) in Iscove's modified Dulbecco's medium (IMDM; Invitrogen, Waltham, MA, USA) supplemented with 10% fetal calf serum (FCS; BioWhittaker, Walkersville, MD, USA), 50 U mL–1 penicillin (Lonza, Basil, Switzerland), 50 μg mL–1 streptomycin (Lonza), and 10 μg mL–1 gentamicin (Lonza) overnight at 4 °C, followed by mechanical separation of the dermis and the epidermis using tweezers. .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation.

Crystallization Assay:

Article Title: Role of the Leucine-rich Repeat (LRR) Domain of Cryopyrin/NALP3 in Monosodium Urate Crystal-induced Inflammation
Article Snippet: Triclinic MSU crystals were prepared under pyrogen-free conditions, using uric acid pretreated for 2 hours at 200°C prior to crystallization ( ). .. Peptidoglycan and R837 were obtained from InVivogen (San Diego, CA), and bacterial RNA was from Ambion (Austin, TX).

Recombinant:

Article Title: Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model
Article Snippet: .. Recombinant mouse IFNαA (PBL Assay Science Co.), GS9620 (Cayman Chemical) and R837 (InvivoGen), were orally or i.p. injected into wt-129 mice infected with EV-A71, at the dose of 300 IU/g (mouse IFNαA), 3.0 μg/g (GS-9620), 4.5μg/g (GS-9620), and 5.0 μg/g (R837), respectively. .. Generation of IL28b KO mouse model Two IFN-lambda knockout (KO) mouse lineages were established in this study.

Injection:

Article Title: Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model
Article Snippet: .. Recombinant mouse IFNαA (PBL Assay Science Co.), GS9620 (Cayman Chemical) and R837 (InvivoGen), were orally or i.p. injected into wt-129 mice infected with EV-A71, at the dose of 300 IU/g (mouse IFNαA), 3.0 μg/g (GS-9620), 4.5μg/g (GS-9620), and 5.0 μg/g (R837), respectively. .. Generation of IL28b KO mouse model Two IFN-lambda knockout (KO) mouse lineages were established in this study.

Derivative Assay:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: .. Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation. .. In the indicated experiments, LCs were obtained by spontaneous migration.

Migration:

Article Title: Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells
Article Snippet: Where indicated, 5 × 104 LCs were cultured for 24 h in 200 µL–1 medium supplemented with 20 μg mL–1 polyinosinic:polycytidylic acid (pI:C; Invivogen, Carlsbad, CA, USA), 20 ng mL–1 lipopolysaccharide (LPS; derived from E. coli , Sigma, St Louis, MO, USA), 5 μg mL–1 R837 (Invivogen), or 5 μg mL–1 R848 (Invivogen) to induce maturation. .. In the indicated experiments, LCs were obtained by spontaneous migration.

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    InvivoGen imiquimod r837
    Imiquimod R837, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imiquimod r837/product/InvivoGen
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    imiquimod r837 - by Bioz Stars, 2020-01
    90/100 stars
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