r centenum strain sw  (ATCC)


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    Structured Review

    ATCC r centenum strain sw
    R Centenum Strain Sw, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r centenum strain sw/product/ATCC
    Average 93 stars, based on 1 article reviews
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    r centenum strain sw - by Bioz Stars, 2024-06
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    r centenum strain sw  (ATCC)


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  • 93

    Structured Review

    ATCC r centenum strain sw
    R Centenum Strain Sw, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r centenum strain sw/product/ATCC
    Average 93 stars, based on 1 article reviews
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    strain r centenum sw  (ATCC)


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    Structured Review

    ATCC strain r centenum sw
    Representative R. <t> centenum </t> mutants used in this study
    Strain R Centenum Sw, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strain r centenum sw/product/ATCC
    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis"

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    Journal:

    doi:

    Representative R.  centenum  mutants used in this study
    Figure Legend Snippet: Representative R. centenum mutants used in this study

    Techniques Used: Mutagenesis, Chemotaxis Assay

    Flow chart depicting the categories of R. centenum mutants isolated in this study.
    Figure Legend Snippet: Flow chart depicting the categories of R. centenum mutants isolated in this study.

    Techniques Used: Isolation

    Western blot analyses of the flagellar types from nonswarming mutants. (A) Flagellar samples collected from cells grown in liquid medium. (B) Flagellar samples collected from cells grown on 0.8% agar plates. Lanes: 1, wild-type R. centenum; 2, ZJH3-9; 3, ZJH3-26; 4, ZJF6-27; 5, ZJH8-3; 6, YB280; 7, ZJP246; 8, ZJG8-11. Protein molecular size markers are labelled on the left.
    Figure Legend Snippet: Western blot analyses of the flagellar types from nonswarming mutants. (A) Flagellar samples collected from cells grown in liquid medium. (B) Flagellar samples collected from cells grown on 0.8% agar plates. Lanes: 1, wild-type R. centenum; 2, ZJH3-9; 3, ZJH3-26; 4, ZJF6-27; 5, ZJH8-3; 6, YB280; 7, ZJP246; 8, ZJG8-11. Protein molecular size markers are labelled on the left.

    Techniques Used: Western Blot

    Southern blot analysis of genomic DNAs from scotophobic and phototactic mutants by probing with an SmaI fragment of the R. centenum chemotaxis gene cluster. (A) Diagram of the che gene cluster and flanking sequences along with relevant restriction sites. (B) Genomic DNA samples digested with ApaI. (C) Genomic DNA samples digested with SmaI. Lengths of DNA markers and fragments are marked in kilobase pairs on the left. W.T., wild-type R. centenum sample. ZJP1177, ZJP1315, ZJA8-25, ZJC2-35, ZJC2-33, ZJC4-20, ZJE6-29, ZJE7-17, ZJF6-4, ZJF7-13, ZJH7-24, ZJI3-1, ZJJ5-26, ZJJ6-5, ZJO3-35, YB300-3, and ZJJ8-2 are samples from the corresponding mutant strains. The loading order in panel C is identical to that in panel B.
    Figure Legend Snippet: Southern blot analysis of genomic DNAs from scotophobic and phototactic mutants by probing with an SmaI fragment of the R. centenum chemotaxis gene cluster. (A) Diagram of the che gene cluster and flanking sequences along with relevant restriction sites. (B) Genomic DNA samples digested with ApaI. (C) Genomic DNA samples digested with SmaI. Lengths of DNA markers and fragments are marked in kilobase pairs on the left. W.T., wild-type R. centenum sample. ZJP1177, ZJP1315, ZJA8-25, ZJC2-35, ZJC2-33, ZJC4-20, ZJE6-29, ZJE7-17, ZJF6-4, ZJF7-13, ZJH7-24, ZJI3-1, ZJJ5-26, ZJJ6-5, ZJO3-35, YB300-3, and ZJJ8-2 are samples from the corresponding mutant strains. The loading order in panel C is identical to that in panel B.

    Techniques Used: Southern Blot, Chemotaxis Assay, Mutagenesis

    Chemotaxis capillary assays for photoperception mutants. The vertical axis represents the relative ratio (see Materials and Methods). Columns: a, wild-type R. centenum strain; b, ZJF6-4; c, ZJF7-13; d, ZJH7-24; e, ZJO3-35; f, R. centenum cheΔ mutant (26).
    Figure Legend Snippet: Chemotaxis capillary assays for photoperception mutants. The vertical axis represents the relative ratio (see Materials and Methods). Columns: a, wild-type R. centenum strain; b, ZJF6-4; c, ZJF7-13; d, ZJH7-24; e, ZJO3-35; f, R. centenum cheΔ mutant (26).

    Techniques Used: Chemotaxis Assay, Mutagenesis

    Phototactic colony migration assays of isolated mutants. (A) Positive swarm colony phototaxis toward an infrared-light source. (B) Negative phototaxis away from a visible-light source. Lanes: w.t., wild-type R. centenum; 1, ZJF6-4; 2, ZJF7-13; 3, ZJH7-24; 4, ZJO3-35; 5, YB300-3; 6, cheΔ mutant.
    Figure Legend Snippet: Phototactic colony migration assays of isolated mutants. (A) Positive swarm colony phototaxis toward an infrared-light source. (B) Negative phototaxis away from a visible-light source. Lanes: w.t., wild-type R. centenum; 1, ZJF6-4; 2, ZJF7-13; 3, ZJH7-24; 4, ZJO3-35; 5, YB300-3; 6, cheΔ mutant.

    Techniques Used: Migration, Isolation, Mutagenesis

    Swarming morphology of wild-type R. centenum versus that of hyperswarming mutant YB600-1. The plates were spotted with equal numbers of cells and incubated in the dark for 40 h at 42°C on PYVS–0.8% agar plates. (A) Wild-type R. centenum. (B) Transposon mutant YB600-1.
    Figure Legend Snippet: Swarming morphology of wild-type R. centenum versus that of hyperswarming mutant YB600-1. The plates were spotted with equal numbers of cells and incubated in the dark for 40 h at 42°C on PYVS–0.8% agar plates. (A) Wild-type R. centenum. (B) Transposon mutant YB600-1.

    Techniques Used: Mutagenesis, Incubation

    strain r centenum sw  (ATCC)


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  • 93

    Structured Review

    ATCC strain r centenum sw
    Representative R. <t> centenum </t> mutants used in this study
    Strain R Centenum Sw, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strain r centenum sw/product/ATCC
    Average 93 stars, based on 1 article reviews
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    strain r centenum sw - by Bioz Stars, 2024-06
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    Images

    1) Product Images from "Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis"

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    Journal:

    doi:

    Representative R.  centenum  mutants used in this study
    Figure Legend Snippet: Representative R. centenum mutants used in this study

    Techniques Used: Mutagenesis, Chemotaxis Assay

    Flow chart depicting the categories of R. centenum mutants isolated in this study.
    Figure Legend Snippet: Flow chart depicting the categories of R. centenum mutants isolated in this study.

    Techniques Used: Isolation

    Western blot analyses of the flagellar types from nonswarming mutants. (A) Flagellar samples collected from cells grown in liquid medium. (B) Flagellar samples collected from cells grown on 0.8% agar plates. Lanes: 1, wild-type R. centenum; 2, ZJH3-9; 3, ZJH3-26; 4, ZJF6-27; 5, ZJH8-3; 6, YB280; 7, ZJP246; 8, ZJG8-11. Protein molecular size markers are labelled on the left.
    Figure Legend Snippet: Western blot analyses of the flagellar types from nonswarming mutants. (A) Flagellar samples collected from cells grown in liquid medium. (B) Flagellar samples collected from cells grown on 0.8% agar plates. Lanes: 1, wild-type R. centenum; 2, ZJH3-9; 3, ZJH3-26; 4, ZJF6-27; 5, ZJH8-3; 6, YB280; 7, ZJP246; 8, ZJG8-11. Protein molecular size markers are labelled on the left.

    Techniques Used: Western Blot

    Southern blot analysis of genomic DNAs from scotophobic and phototactic mutants by probing with an SmaI fragment of the R. centenum chemotaxis gene cluster. (A) Diagram of the che gene cluster and flanking sequences along with relevant restriction sites. (B) Genomic DNA samples digested with ApaI. (C) Genomic DNA samples digested with SmaI. Lengths of DNA markers and fragments are marked in kilobase pairs on the left. W.T., wild-type R. centenum sample. ZJP1177, ZJP1315, ZJA8-25, ZJC2-35, ZJC2-33, ZJC4-20, ZJE6-29, ZJE7-17, ZJF6-4, ZJF7-13, ZJH7-24, ZJI3-1, ZJJ5-26, ZJJ6-5, ZJO3-35, YB300-3, and ZJJ8-2 are samples from the corresponding mutant strains. The loading order in panel C is identical to that in panel B.
    Figure Legend Snippet: Southern blot analysis of genomic DNAs from scotophobic and phototactic mutants by probing with an SmaI fragment of the R. centenum chemotaxis gene cluster. (A) Diagram of the che gene cluster and flanking sequences along with relevant restriction sites. (B) Genomic DNA samples digested with ApaI. (C) Genomic DNA samples digested with SmaI. Lengths of DNA markers and fragments are marked in kilobase pairs on the left. W.T., wild-type R. centenum sample. ZJP1177, ZJP1315, ZJA8-25, ZJC2-35, ZJC2-33, ZJC4-20, ZJE6-29, ZJE7-17, ZJF6-4, ZJF7-13, ZJH7-24, ZJI3-1, ZJJ5-26, ZJJ6-5, ZJO3-35, YB300-3, and ZJJ8-2 are samples from the corresponding mutant strains. The loading order in panel C is identical to that in panel B.

    Techniques Used: Southern Blot, Chemotaxis Assay, Mutagenesis

    Chemotaxis capillary assays for photoperception mutants. The vertical axis represents the relative ratio (see Materials and Methods). Columns: a, wild-type R. centenum strain; b, ZJF6-4; c, ZJF7-13; d, ZJH7-24; e, ZJO3-35; f, R. centenum cheΔ mutant (26).
    Figure Legend Snippet: Chemotaxis capillary assays for photoperception mutants. The vertical axis represents the relative ratio (see Materials and Methods). Columns: a, wild-type R. centenum strain; b, ZJF6-4; c, ZJF7-13; d, ZJH7-24; e, ZJO3-35; f, R. centenum cheΔ mutant (26).

    Techniques Used: Chemotaxis Assay, Mutagenesis

    Phototactic colony migration assays of isolated mutants. (A) Positive swarm colony phototaxis toward an infrared-light source. (B) Negative phototaxis away from a visible-light source. Lanes: w.t., wild-type R. centenum; 1, ZJF6-4; 2, ZJF7-13; 3, ZJH7-24; 4, ZJO3-35; 5, YB300-3; 6, cheΔ mutant.
    Figure Legend Snippet: Phototactic colony migration assays of isolated mutants. (A) Positive swarm colony phototaxis toward an infrared-light source. (B) Negative phototaxis away from a visible-light source. Lanes: w.t., wild-type R. centenum; 1, ZJF6-4; 2, ZJF7-13; 3, ZJH7-24; 4, ZJO3-35; 5, YB300-3; 6, cheΔ mutant.

    Techniques Used: Migration, Isolation, Mutagenesis

    Swarming morphology of wild-type R. centenum versus that of hyperswarming mutant YB600-1. The plates were spotted with equal numbers of cells and incubated in the dark for 40 h at 42°C on PYVS–0.8% agar plates. (A) Wild-type R. centenum. (B) Transposon mutant YB600-1.
    Figure Legend Snippet: Swarming morphology of wild-type R. centenum versus that of hyperswarming mutant YB600-1. The plates were spotted with equal numbers of cells and incubated in the dark for 40 h at 42°C on PYVS–0.8% agar plates. (A) Wild-type R. centenum. (B) Transposon mutant YB600-1.

    Techniques Used: Mutagenesis, Incubation

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    ATCC r centenum strain sw
    R Centenum Strain Sw, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r centenum strain sw/product/ATCC
    Average 93 stars, based on 1 article reviews
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    r centenum strain sw - by Bioz Stars, 2024-06
    93/100 stars
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    93
    ATCC strain r centenum sw
    Representative R. <t> centenum </t> mutants used in this study
    Strain R Centenum Sw, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strain r centenum sw/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strain r centenum sw - by Bioz Stars, 2024-06
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    Representative R.  centenum  mutants used in this study

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Representative R. centenum mutants used in this study

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Mutagenesis, Chemotaxis Assay

    Flow chart depicting the categories of R. centenum mutants isolated in this study.

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Flow chart depicting the categories of R. centenum mutants isolated in this study.

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Isolation

    Western blot analyses of the flagellar types from nonswarming mutants. (A) Flagellar samples collected from cells grown in liquid medium. (B) Flagellar samples collected from cells grown on 0.8% agar plates. Lanes: 1, wild-type R. centenum; 2, ZJH3-9; 3, ZJH3-26; 4, ZJF6-27; 5, ZJH8-3; 6, YB280; 7, ZJP246; 8, ZJG8-11. Protein molecular size markers are labelled on the left.

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Western blot analyses of the flagellar types from nonswarming mutants. (A) Flagellar samples collected from cells grown in liquid medium. (B) Flagellar samples collected from cells grown on 0.8% agar plates. Lanes: 1, wild-type R. centenum; 2, ZJH3-9; 3, ZJH3-26; 4, ZJF6-27; 5, ZJH8-3; 6, YB280; 7, ZJP246; 8, ZJG8-11. Protein molecular size markers are labelled on the left.

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Western Blot

    Southern blot analysis of genomic DNAs from scotophobic and phototactic mutants by probing with an SmaI fragment of the R. centenum chemotaxis gene cluster. (A) Diagram of the che gene cluster and flanking sequences along with relevant restriction sites. (B) Genomic DNA samples digested with ApaI. (C) Genomic DNA samples digested with SmaI. Lengths of DNA markers and fragments are marked in kilobase pairs on the left. W.T., wild-type R. centenum sample. ZJP1177, ZJP1315, ZJA8-25, ZJC2-35, ZJC2-33, ZJC4-20, ZJE6-29, ZJE7-17, ZJF6-4, ZJF7-13, ZJH7-24, ZJI3-1, ZJJ5-26, ZJJ6-5, ZJO3-35, YB300-3, and ZJJ8-2 are samples from the corresponding mutant strains. The loading order in panel C is identical to that in panel B.

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Southern blot analysis of genomic DNAs from scotophobic and phototactic mutants by probing with an SmaI fragment of the R. centenum chemotaxis gene cluster. (A) Diagram of the che gene cluster and flanking sequences along with relevant restriction sites. (B) Genomic DNA samples digested with ApaI. (C) Genomic DNA samples digested with SmaI. Lengths of DNA markers and fragments are marked in kilobase pairs on the left. W.T., wild-type R. centenum sample. ZJP1177, ZJP1315, ZJA8-25, ZJC2-35, ZJC2-33, ZJC4-20, ZJE6-29, ZJE7-17, ZJF6-4, ZJF7-13, ZJH7-24, ZJI3-1, ZJJ5-26, ZJJ6-5, ZJO3-35, YB300-3, and ZJJ8-2 are samples from the corresponding mutant strains. The loading order in panel C is identical to that in panel B.

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Southern Blot, Chemotaxis Assay, Mutagenesis

    Chemotaxis capillary assays for photoperception mutants. The vertical axis represents the relative ratio (see Materials and Methods). Columns: a, wild-type R. centenum strain; b, ZJF6-4; c, ZJF7-13; d, ZJH7-24; e, ZJO3-35; f, R. centenum cheΔ mutant (26).

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Chemotaxis capillary assays for photoperception mutants. The vertical axis represents the relative ratio (see Materials and Methods). Columns: a, wild-type R. centenum strain; b, ZJF6-4; c, ZJF7-13; d, ZJH7-24; e, ZJO3-35; f, R. centenum cheΔ mutant (26).

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Chemotaxis Assay, Mutagenesis

    Phototactic colony migration assays of isolated mutants. (A) Positive swarm colony phototaxis toward an infrared-light source. (B) Negative phototaxis away from a visible-light source. Lanes: w.t., wild-type R. centenum; 1, ZJF6-4; 2, ZJF7-13; 3, ZJH7-24; 4, ZJO3-35; 5, YB300-3; 6, cheΔ mutant.

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Phototactic colony migration assays of isolated mutants. (A) Positive swarm colony phototaxis toward an infrared-light source. (B) Negative phototaxis away from a visible-light source. Lanes: w.t., wild-type R. centenum; 1, ZJF6-4; 2, ZJF7-13; 3, ZJH7-24; 4, ZJO3-35; 5, YB300-3; 6, cheΔ mutant.

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Migration, Isolation, Mutagenesis

    Swarming morphology of wild-type R. centenum versus that of hyperswarming mutant YB600-1. The plates were spotted with equal numbers of cells and incubated in the dark for 40 h at 42°C on PYVS–0.8% agar plates. (A) Wild-type R. centenum. (B) Transposon mutant YB600-1.

    Journal:

    Article Title: Isolation of Rhodospirillum centenum Mutants Defective in Phototactic Colony Motility by Transposon Mutagenesis

    doi:

    Figure Lengend Snippet: Swarming morphology of wild-type R. centenum versus that of hyperswarming mutant YB600-1. The plates were spotted with equal numbers of cells and incubated in the dark for 40 h at 42°C on PYVS–0.8% agar plates. (A) Wild-type R. centenum. (B) Transposon mutant YB600-1.

    Article Snippet: Wild-type swarming strain R. centenum SW (ATCC 51521) is the parental strain used in this study ( 12 , 37 ).

    Techniques: Mutagenesis, Incubation