Journal: The Journal of Neuroscience

Article Title: Extracellular Calcium Controls the Expression of Two Different Forms of Ripple-Like Hippocampal Oscillations

doi: 10.1523/JNEUROSCI.2826-13.2014

Figure Lengend Snippet: Spontaneous EPSC and IPSC dynamics in 3 mm and 1 mm Ca2+. A1, Spontaneous synaptic potentials were recorded from identified CA3 pyramidal cells at different membrane holding levels to detect excitatory (EPSCs) and inhibitory (IPSCs) components in 3 mm (n = 6 cells) and 1 mm (n = 6 cells) Ca2+. B1, Cumulative distribution of the interevent interval of spontaneous EPSCs (left) and spontaneous IPSCs (right) recorded at −60 mV in the two different Ca2+ concentrations. C1, Group data of mean frequency for spontaneous EPSCs (left) and IPSCs (right) as detected at −60 mV with standard pipette solutions (see Materials and Methods) and at different potentials with QX314. D1, Group data of mean amplitude for spontaneous EPSCs (left) and IPSCs (right). E1, Group data of total charge for spontaneous EPSCs (left) and IPSCs (right). A2, Spontaneous synaptic potentials recorded in identified interneurons in 3 mm (n = 6) and 1 mm (n = 4) Ca2+. B2, Cumulative distribution of the interevent interval of spontaneous EPSCs (left) and IPSCs (right) recorded from interneurons at the two different Ca2+ concentrations. C2, Group data of mean frequency for spontaneous EPSCs (left) and IPSCs (right). D2, Group data of mean amplitude for spontaneous EPSCs (left) and IPSCs (right). *p < 0.05; **p < 0.01. E2, Group data of total charge for spontaneous EPSCs (left) and IPSCs (right).

Article Snippet: In a set of experiments, we included QX314-chloride (5 m m ; Alomone Labs) in the pipette to improve voltage-clamp conditions and to minimize voltage-dependent conductances at more depolarized holding potentials.

Techniques: Transferring

Journal: PLoS ONE

Article Title: Dopamine Preferentially Inhibits NMDA Receptor-Mediated EPSCs by Acting on Presynaptic D 1 Receptors in Nucleus Accumbens during Postnatal Development

doi: 10.1371/journal.pone.0086970

Figure Lengend Snippet: A : Comparison of the average change in amplitude of the early component of the glutamatergic EPSCs (in pA) measured at −60 mV, on superfusion of DA in the presence and absence of QX314. The solid bar represents the average response in 80 neurons (n = 80), in the presence of QX314 and the dashed bar represents the average of 8 neurons (n = 8) in the absence of QX314. No statistically significant difference was found between the two groups (Paired Student’s t test p<0.050, p = 0.471). B : Comparison of the average change in amplitude of the late component of the glutamatergic EPSCs (in pA) measured at −20 mV, on superfusion of DA in the presence and absence of QX314. The solid bar represents the average of 70 neurons (n = 70), in the presence of QX314 and the dashed bar represents the average of 7 neurons (n = 7) in the absence of QX314. No statistically significant difference was found between the two groups (Paired Student’s t test p<0.048, p = 0.500).

Article Snippet: QX314 (5 mM; Alomone Laboratories, Jerusalem, Israel) was routinely added to the recording pipette solution to prevent voltage-sensitive Na + channels from generating action potentials.

Techniques: