kb quick load dna ladders  (New England Biolabs)


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    Name:
    Quick Load 1 kb DNA Ladder
    Description:
    Quick Load 1 kb DNA Ladder 375 gel lanes
    Catalog Number:
    N0468L
    Price:
    153
    Category:
    DNA Ladders
    Size:
    375 gel lanes
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    New England Biolabs kb quick load dna ladders
    Quick Load 1 kb DNA Ladder
    Quick Load 1 kb DNA Ladder 375 gel lanes
    https://www.bioz.com/result/kb quick load dna ladders/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kb quick load dna ladders - by Bioz Stars, 2021-05
    96/100 stars

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    Staining:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Polymerase Chain Reaction:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Purification:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Liquid Chromatography:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Fast Protein Liquid Chromatography:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Gas Chromatography:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Plasmid Preparation:

    Article Title: Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Article Snippet: Ice LB Miller broth powdered (Fisher Scientific, catalog number: BP1426-2) Ampicillin sodium salt powdered (Sigma-Aldrich, catalog number: A9518) Note: Prepare 100 mM stock (1,000×) in deionized water. .. Plasmid DNA Extraction Miniprep Kit (GeneJET) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: K0502) Bam HI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0054); store at −20 °C Restriction enzyme buffer FastDigest (10×; 5× 1 ml) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: B64); store at −20 °C Eco RI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0274); store at −20 °C Quick-Load 1 kb DNA ladder (New England Biolabs) 5× nucleic acid loading buffer (10 ml, premixed) (Bio-Rad Laboratories, catalog number: 1610767) Deionized water Acrylamide electrophoresis gels (Criterion TGX Precast Gels; 18 well comb, 30 µl loading volume per well, 1.0 mm thickness, 4–20% gel percentage) (Bio-Rad Laboratories, catalog number: 5671094) Agar powdered (Fisher Scientific, catalog number: BP24662) 1× TAE running buffer Ethidium bromide (10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) Glycerol 100% (EMD Millipore, catalog number: GX0185) Isopropyl β-D-1-thiogalactopyranoside (IPTG) powdered (Gold Bio, catalog number: I2481C100) Note: Prepare 10 ml of 1 M (10,000×) stock in deionized water and store at −20 °C . .. Zinc chloride (ZnCl2 ), 99.999% trace metals basis powdered (Sigma-Aldrich, catalog number: 229997) Note: Prepare as 100 mM stock in deionized water .

    DNA Extraction:

    Article Title: Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Article Snippet: Ice LB Miller broth powdered (Fisher Scientific, catalog number: BP1426-2) Ampicillin sodium salt powdered (Sigma-Aldrich, catalog number: A9518) Note: Prepare 100 mM stock (1,000×) in deionized water. .. Plasmid DNA Extraction Miniprep Kit (GeneJET) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: K0502) Bam HI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0054); store at −20 °C Restriction enzyme buffer FastDigest (10×; 5× 1 ml) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: B64); store at −20 °C Eco RI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0274); store at −20 °C Quick-Load 1 kb DNA ladder (New England Biolabs) 5× nucleic acid loading buffer (10 ml, premixed) (Bio-Rad Laboratories, catalog number: 1610767) Deionized water Acrylamide electrophoresis gels (Criterion TGX Precast Gels; 18 well comb, 30 µl loading volume per well, 1.0 mm thickness, 4–20% gel percentage) (Bio-Rad Laboratories, catalog number: 5671094) Agar powdered (Fisher Scientific, catalog number: BP24662) 1× TAE running buffer Ethidium bromide (10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) Glycerol 100% (EMD Millipore, catalog number: GX0185) Isopropyl β-D-1-thiogalactopyranoside (IPTG) powdered (Gold Bio, catalog number: I2481C100) Note: Prepare 10 ml of 1 M (10,000×) stock in deionized water and store at −20 °C . .. Zinc chloride (ZnCl2 ), 99.999% trace metals basis powdered (Sigma-Aldrich, catalog number: 229997) Note: Prepare as 100 mM stock in deionized water .

    Electrophoresis:

    Article Title: Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Article Snippet: Ice LB Miller broth powdered (Fisher Scientific, catalog number: BP1426-2) Ampicillin sodium salt powdered (Sigma-Aldrich, catalog number: A9518) Note: Prepare 100 mM stock (1,000×) in deionized water. .. Plasmid DNA Extraction Miniprep Kit (GeneJET) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: K0502) Bam HI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0054); store at −20 °C Restriction enzyme buffer FastDigest (10×; 5× 1 ml) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: B64); store at −20 °C Eco RI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0274); store at −20 °C Quick-Load 1 kb DNA ladder (New England Biolabs) 5× nucleic acid loading buffer (10 ml, premixed) (Bio-Rad Laboratories, catalog number: 1610767) Deionized water Acrylamide electrophoresis gels (Criterion TGX Precast Gels; 18 well comb, 30 µl loading volume per well, 1.0 mm thickness, 4–20% gel percentage) (Bio-Rad Laboratories, catalog number: 5671094) Agar powdered (Fisher Scientific, catalog number: BP24662) 1× TAE running buffer Ethidium bromide (10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) Glycerol 100% (EMD Millipore, catalog number: GX0185) Isopropyl β-D-1-thiogalactopyranoside (IPTG) powdered (Gold Bio, catalog number: I2481C100) Note: Prepare 10 ml of 1 M (10,000×) stock in deionized water and store at −20 °C . .. Zinc chloride (ZnCl2 ), 99.999% trace metals basis powdered (Sigma-Aldrich, catalog number: 229997) Note: Prepare as 100 mM stock in deionized water .

    Article Title: Mapping of histone modifications in episomal HBV cccDNA uncovers an unusual chromatin organization amenable to epigenetic manipulation
    Article Snippet: .. For Southern blot analysis, 0.5 μL of Quick-Load 1-kb DNA ladder (New England Biolabs) and 15 μg ( ) or 25 μg ( ) of HIRT-extracted DNA were loaded per lane and separated by electrophoresis in a 1.2% (wt/vol) agarose gel in 1× Tris-Acetate-EDTA buffer. .. After electrophoresis the DNA was depurinated, denatured, and neutralized exactly as described by Cai et al. ( ) and transferred onto Hybond XL membrane (Amersham) by using the TurboBlotter system (GE Healthcare). (-) strand HBV DNA was detected with a DIG-labeled (+) strand HBV RNA probe transcribed from a ScaI-linearized pGEM3Z-HBV plasmid with DIG Northern Starter Kit (Roche) according to manufacturer’s instructions.

    Marker:

    Article Title: D-Serine Metabolism and Its Importance in Development of Dictyostelium discoideum
    Article Snippet: Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2018.00784/full#supplementary-material FIGURE S1 Construction of dsd -null mutant. (A) The blasticidin S resistance gene (bsr ) was introduced into the Wild-type (WT) strain of Dictyostelium discoideum by homologous recombination. (B) Agarose gel electrophoresis of PCR-amplified DNA with DsdKOckf (a) and DsdKOckr (b) primers from the parental strain (WT) and the dsd -null mutants. .. The sizes of marker DNA fragments (Quick-Load 1 kB DNA ladder; New England BioLabs) are shown on the left. ..

    Southern Blot:

    Article Title: Mapping of histone modifications in episomal HBV cccDNA uncovers an unusual chromatin organization amenable to epigenetic manipulation
    Article Snippet: .. For Southern blot analysis, 0.5 μL of Quick-Load 1-kb DNA ladder (New England Biolabs) and 15 μg ( ) or 25 μg ( ) of HIRT-extracted DNA were loaded per lane and separated by electrophoresis in a 1.2% (wt/vol) agarose gel in 1× Tris-Acetate-EDTA buffer. .. After electrophoresis the DNA was depurinated, denatured, and neutralized exactly as described by Cai et al. ( ) and transferred onto Hybond XL membrane (Amersham) by using the TurboBlotter system (GE Healthcare). (-) strand HBV DNA was detected with a DIG-labeled (+) strand HBV RNA probe transcribed from a ScaI-linearized pGEM3Z-HBV plasmid with DIG Northern Starter Kit (Roche) according to manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: Mapping of histone modifications in episomal HBV cccDNA uncovers an unusual chromatin organization amenable to epigenetic manipulation
    Article Snippet: .. For Southern blot analysis, 0.5 μL of Quick-Load 1-kb DNA ladder (New England Biolabs) and 15 μg ( ) or 25 μg ( ) of HIRT-extracted DNA were loaded per lane and separated by electrophoresis in a 1.2% (wt/vol) agarose gel in 1× Tris-Acetate-EDTA buffer. .. After electrophoresis the DNA was depurinated, denatured, and neutralized exactly as described by Cai et al. ( ) and transferred onto Hybond XL membrane (Amersham) by using the TurboBlotter system (GE Healthcare). (-) strand HBV DNA was detected with a DIG-labeled (+) strand HBV RNA probe transcribed from a ScaI-linearized pGEM3Z-HBV plasmid with DIG Northern Starter Kit (Roche) according to manufacturer’s instructions.

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Article Title: Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
    Article Snippet: .. Expression of Als1 and Als2 mutations Results of the cDNA cloning of the Als1 and Als2 mutations are shown in the 1% Tris-borate-EDTA (TBE) agarose gel in Fig. [30 min at 100 V, 1 kb ladder (NEB Cat. No. N0468S)]. .. The amplified cDNA fragments appear to match the expected fragment sizes of 2066 bp and 1942 bp for Als1 (see Fig. , lanes marked ‘a’ and ‘b’ respectively) and 2083 bp and 1957 bp for Als2 (see Fig. , lanes marked ‘c’ and ‘d’ respectively).

    Nucleic Acid Electrophoresis:

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Amplification:

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Negative Control:

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Expressing:

    Article Title: Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
    Article Snippet: .. Expression of Als1 and Als2 mutations Results of the cDNA cloning of the Als1 and Als2 mutations are shown in the 1% Tris-borate-EDTA (TBE) agarose gel in Fig. [30 min at 100 V, 1 kb ladder (NEB Cat. No. N0468S)]. .. The amplified cDNA fragments appear to match the expected fragment sizes of 2066 bp and 1942 bp for Als1 (see Fig. , lanes marked ‘a’ and ‘b’ respectively) and 2083 bp and 1957 bp for Als2 (see Fig. , lanes marked ‘c’ and ‘d’ respectively).

    Clone Assay:

    Article Title: Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
    Article Snippet: .. Expression of Als1 and Als2 mutations Results of the cDNA cloning of the Als1 and Als2 mutations are shown in the 1% Tris-borate-EDTA (TBE) agarose gel in Fig. [30 min at 100 V, 1 kb ladder (NEB Cat. No. N0468S)]. .. The amplified cDNA fragments appear to match the expected fragment sizes of 2066 bp and 1942 bp for Als1 (see Fig. , lanes marked ‘a’ and ‘b’ respectively) and 2083 bp and 1957 bp for Als2 (see Fig. , lanes marked ‘c’ and ‘d’ respectively).

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    New England Biolabs neb quick load purple
    Tt Ago can cut linearized pUC19 in the presence of ET SSB. Tt Ago is able to cleave linear dsDNA in the presence of ET SSB. pUC19 (2686 bp) was linearized with SspI and used as a substrate for the reaction. Guides pUC19-1 and pUC19-2 (Supporting information S1 Table ) were designed to cut near the BamHI restriction site on pUC19 to generate a 608 bp fragment. Lane 1) <t>NEB</t> Quick-Load Purple 2-Log <t>DNA</t> Ladder (0.1–10.0 kb); 2) Tt Ago + ET SSB; 3) Tt Ago only; 4) SspI linearized pUC19 control.
    Neb Quick Load Purple, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb quick load purple/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb quick load purple - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs quick load 1 kb extend dna ladder
    Quality assessment of genomic DNA of shrimp muscle extracted with different DNA extraction methods. After purified by AMPure PB bead, gDNA (n=4) extracted from (A) CTAB method (100 ng  of gDNA), (B) QIAGEN Genomic-tip 100/G kit (100 ng of  gDNA), (C) E.Z.N.A. ® Mollusc DNA Kit (200 ng of  gDNA), (D) TIANamp Marine Animals DNA kit (100 ng of gDNA) and (E) Sbeadex livestock kit (200 ng of  gDNA) were loaded on 0.75% pulsed-field gel electrophoresis and run at 80 Volts for 9 h. The DNA size marker is Quick-Load 1 kb Extend DNA Ladder (New England BioLabs).
    Quick Load 1 Kb Extend Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick load 1 kb extend dna ladder/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick load 1 kb extend dna ladder - by Bioz Stars, 2021-05
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    Tt Ago can cut linearized pUC19 in the presence of ET SSB. Tt Ago is able to cleave linear dsDNA in the presence of ET SSB. pUC19 (2686 bp) was linearized with SspI and used as a substrate for the reaction. Guides pUC19-1 and pUC19-2 (Supporting information S1 Table ) were designed to cut near the BamHI restriction site on pUC19 to generate a 608 bp fragment. Lane 1) NEB Quick-Load Purple 2-Log DNA Ladder (0.1–10.0 kb); 2) Tt Ago + ET SSB; 3) Tt Ago only; 4) SspI linearized pUC19 control.

    Journal: PLoS ONE

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro

    doi: 10.1371/journal.pone.0203073

    Figure Lengend Snippet: Tt Ago can cut linearized pUC19 in the presence of ET SSB. Tt Ago is able to cleave linear dsDNA in the presence of ET SSB. pUC19 (2686 bp) was linearized with SspI and used as a substrate for the reaction. Guides pUC19-1 and pUC19-2 (Supporting information S1 Table ) were designed to cut near the BamHI restriction site on pUC19 to generate a 608 bp fragment. Lane 1) NEB Quick-Load Purple 2-Log DNA Ladder (0.1–10.0 kb); 2) Tt Ago + ET SSB; 3) Tt Ago only; 4) SspI linearized pUC19 control.

    Article Snippet: The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file.

    Techniques:

    Quality assessment of genomic DNA of shrimp muscle extracted with different DNA extraction methods. After purified by AMPure PB bead, gDNA (n=4) extracted from (A) CTAB method (100 ng  of gDNA), (B) QIAGEN Genomic-tip 100/G kit (100 ng of  gDNA), (C) E.Z.N.A. ® Mollusc DNA Kit (200 ng of  gDNA), (D) TIANamp Marine Animals DNA kit (100 ng of gDNA) and (E) Sbeadex livestock kit (200 ng of  gDNA) were loaded on 0.75% pulsed-field gel electrophoresis and run at 80 Volts for 9 h. The DNA size marker is Quick-Load 1 kb Extend DNA Ladder (New England BioLabs).

    Journal: PeerJ

    Article Title: Optimization of high molecular weight DNA extraction methods in shrimp for a long-read sequencing platform

    doi: 10.7717/peerj.10340

    Figure Lengend Snippet: Quality assessment of genomic DNA of shrimp muscle extracted with different DNA extraction methods. After purified by AMPure PB bead, gDNA (n=4) extracted from (A) CTAB method (100 ng of gDNA), (B) QIAGEN Genomic-tip 100/G kit (100 ng of gDNA), (C) E.Z.N.A. ® Mollusc DNA Kit (200 ng of gDNA), (D) TIANamp Marine Animals DNA kit (100 ng of gDNA) and (E) Sbeadex livestock kit (200 ng of gDNA) were loaded on 0.75% pulsed-field gel electrophoresis and run at 80 Volts for 9 h. The DNA size marker is Quick-Load 1 kb Extend DNA Ladder (New England BioLabs).

    Article Snippet: Supplemental Information 10.7717/peerj.10340/supp-1 Quality assessment of genomic DNA of shrimp muscle extracted with different DNA extraction methods After purified by AMPure PB bead, 100 ng of gDNA extracted from (A) CTAB method, (B) QIAGEN Genomic-tip 100/G kit, (C) E.Z.N.A.® Mollusc DNA Kit, (D) TIANamp Marine Animals DNA kit and (E) Sbeadex livestock kit were loaded on 0.75% Pulsed-field gel electrophoresis and run at 80 Volts for 9 h. The DNA size marker is Quick-Load 1 kb Extend DNA Ladder (NEW ENGLAND BioLabs).

    Techniques: DNA Extraction, Purification, Pulsed-Field Gel, Electrophoresis, Marker