qubit rna hs assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Qubit RNA HS Assay Kit
    Description:
    The Qubit RNA HS Assay Kit when used with the Qubit Fluorometer provides an accurate and selective method for the quantitation of low abundance RNA samples The assay is highly selective for RNA and will not quantitate DNA protein or free nucleotides Common contaminants such as salts free nucleotides solvents detergents or protein are well tolerated in the assay The assay kit is designed to be accurate for RNA sample concentrations between 250 pg µL and 100 ng µL The kit provides concentrated assay reagent dilution buffer and pre diluted RNA standards Simply dilute the reagent using the buffer provided add your sample any volume between 1 µL and 20 µL is acceptable and read the concentration using the Qubit Fluorometer Which product to choose for RNA HS High Sensitivity quantitation • For 1 20 samples use this Qubit RNA HS Assay Kit with the Qubit Fluorometer • For 20 2000 samples use the Quant iT RNA HS Assay Kit with microplate readerNotes 1 All Qubit assay kits can be used with the Qubit 1 0 Qubit 2 0 Qubit 3 and Qubit 4 fluorometers 2 500 µL thin walled PCR tubes are required but not included
    Catalog Number:
    q32852
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|RNA Quantitation|Nucleic Acid Quantitation
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher qubit rna hs assay kit
    Quantitative and qualitative comparison of <t>RNA</t> recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using <t>Qubit</t> fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.
    The Qubit RNA HS Assay Kit when used with the Qubit Fluorometer provides an accurate and selective method for the quantitation of low abundance RNA samples The assay is highly selective for RNA and will not quantitate DNA protein or free nucleotides Common contaminants such as salts free nucleotides solvents detergents or protein are well tolerated in the assay The assay kit is designed to be accurate for RNA sample concentrations between 250 pg µL and 100 ng µL The kit provides concentrated assay reagent dilution buffer and pre diluted RNA standards Simply dilute the reagent using the buffer provided add your sample any volume between 1 µL and 20 µL is acceptable and read the concentration using the Qubit Fluorometer Which product to choose for RNA HS High Sensitivity quantitation • For 1 20 samples use this Qubit RNA HS Assay Kit with the Qubit Fluorometer • For 20 2000 samples use the Quant iT RNA HS Assay Kit with microplate readerNotes 1 All Qubit assay kits can be used with the Qubit 1 0 Qubit 2 0 Qubit 3 and Qubit 4 fluorometers 2 500 µL thin walled PCR tubes are required but not included
    https://www.bioz.com/result/qubit rna hs assay kit/product/Thermo Fisher
    Average 99 stars, based on 438 article reviews
    Price from $9.99 to $1999.99
    qubit rna hs assay kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes"

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

    Journal: Scientific Reports

    doi: 10.1038/srep41114

    Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.
    Figure Legend Snippet: Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

    Techniques Used: Isolation, Quantitation Assay, RNA HS Assay, Software, Fluorescence

    2) Product Images from "Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine"

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine

    Journal: BMC Cancer

    doi: 10.1186/s12885-016-2930-9

    a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5 × 10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls
    Figure Legend Snippet: a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5 × 10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Techniques Used: RNA Extraction, Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction

    3) Product Images from "Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?"

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

    Journal: BMC Clinical Pathology

    doi: 10.1186/s12907-016-0039-3

    Comparison of Qubit RNA yields obtained using four off the shelf extraction kits: Geometric mean RNA yield and associated geometric standard error of mean for the four extraction kits tested in this study, n = 30 for each method. P-values show the statistical significance of the difference in yield between each method
    Figure Legend Snippet: Comparison of Qubit RNA yields obtained using four off the shelf extraction kits: Geometric mean RNA yield and associated geometric standard error of mean for the four extraction kits tested in this study, n = 30 for each method. P-values show the statistical significance of the difference in yield between each method

    Techniques Used:

    4) Product Images from "Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome"

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome

    Journal: Nature

    doi: 10.1038/nature25157

    Increased fitness of evolved synthetic nucleocapsids Evolution drastically increases the property under selection without compromising previously evolved properties. a-c . Time courses of full-length RNA genomes per 1000 capsids isolated after challenge: a . 10 μg/mL RNase A at 37 °C (RNase, n = 3 independent reactions), b . Heparinized whole murine blood at 37°C (Blood, n = 3 independent reactions), and c . in vivo circulation in mice (Live mouse, n = 5 biologically independent animals). Error bars represent standard error of the mean. d . Summary of improved nucleocapsid properties, including total packaged RNA (10 μg/mL RNase A for 10 min at 25 °C to degrade non-encapsulated RNA, n = 3 independent reactions). The colored arrows in a-c indicate the 6-hour time point represented in the summary plot. Five synthetic nucleocapsids were tested: I53-50-v0 (original assembly which did not package its full length mRNA), I53-50-v1 (design with positive interior surface for packaging RNA), I53-50-v2 (evolution-optimized interior surface), I53-50-v3 (evolution-optimized residues lining the capsid pore), and I53-50-v4 (evolution-optimized exterior surface for increased circulation in living mice). Evolution resulted in efficient genome encapsulation for I53-50-v2 and its derivatives (approximately 1 RNA genome per 14 icosahedral capsids for I53-50-v2), protection from blood for I53-50-v3 and I53-50-v4 (82% and 71% protection, respectively), and increased circulation half-life for I53-50-v4 (4.5 hours serum half-life). Full-length RNA genomes were quantitated by RT-qPCR, capsid proteins were quantitated by Qubit, and genomes per capsid were calculated based on these values by dividing the number of genomes by the number of capsids. e . Nucleocapsid genomes are enriched and ribosomal RNA is depleted in nucleocapsids. f . Top 13 RNA transcripts encapsulated in I53-50-v4. Nucleocapsid genomes account for more than 74% of the packaged transcripts. g,h . The relative biodistribution of intact I53-50-v3 ( g ) and I53-50-v4 ( h ) nucleocapsids was evaluated by RT-qPCR of their full-length genomes recovered from mouse organs harvested 5 minutes or 4 hours after retro-orbital injection (n = 3 biologically independent animals at each time point for each nucleocapsid, I53-50-v3 and I53-50-v4). Red (5 minutes) and blue (240 minutes) bars represent the mean of three biologically independent animals, error bars represent the standard error of the mean, and thick black bars represent the detection limit of the assay. No obvious tissue tropism was observed for either nucleocapsid. At four hours post injection, I53-50-v3 had largely disappeared, while I53-50-v4 remained predominantly in the blood with lower levels in the other tissues.
    Figure Legend Snippet: Increased fitness of evolved synthetic nucleocapsids Evolution drastically increases the property under selection without compromising previously evolved properties. a-c . Time courses of full-length RNA genomes per 1000 capsids isolated after challenge: a . 10 μg/mL RNase A at 37 °C (RNase, n = 3 independent reactions), b . Heparinized whole murine blood at 37°C (Blood, n = 3 independent reactions), and c . in vivo circulation in mice (Live mouse, n = 5 biologically independent animals). Error bars represent standard error of the mean. d . Summary of improved nucleocapsid properties, including total packaged RNA (10 μg/mL RNase A for 10 min at 25 °C to degrade non-encapsulated RNA, n = 3 independent reactions). The colored arrows in a-c indicate the 6-hour time point represented in the summary plot. Five synthetic nucleocapsids were tested: I53-50-v0 (original assembly which did not package its full length mRNA), I53-50-v1 (design with positive interior surface for packaging RNA), I53-50-v2 (evolution-optimized interior surface), I53-50-v3 (evolution-optimized residues lining the capsid pore), and I53-50-v4 (evolution-optimized exterior surface for increased circulation in living mice). Evolution resulted in efficient genome encapsulation for I53-50-v2 and its derivatives (approximately 1 RNA genome per 14 icosahedral capsids for I53-50-v2), protection from blood for I53-50-v3 and I53-50-v4 (82% and 71% protection, respectively), and increased circulation half-life for I53-50-v4 (4.5 hours serum half-life). Full-length RNA genomes were quantitated by RT-qPCR, capsid proteins were quantitated by Qubit, and genomes per capsid were calculated based on these values by dividing the number of genomes by the number of capsids. e . Nucleocapsid genomes are enriched and ribosomal RNA is depleted in nucleocapsids. f . Top 13 RNA transcripts encapsulated in I53-50-v4. Nucleocapsid genomes account for more than 74% of the packaged transcripts. g,h . The relative biodistribution of intact I53-50-v3 ( g ) and I53-50-v4 ( h ) nucleocapsids was evaluated by RT-qPCR of their full-length genomes recovered from mouse organs harvested 5 minutes or 4 hours after retro-orbital injection (n = 3 biologically independent animals at each time point for each nucleocapsid, I53-50-v3 and I53-50-v4). Red (5 minutes) and blue (240 minutes) bars represent the mean of three biologically independent animals, error bars represent the standard error of the mean, and thick black bars represent the detection limit of the assay. No obvious tissue tropism was observed for either nucleocapsid. At four hours post injection, I53-50-v3 had largely disappeared, while I53-50-v4 remained predominantly in the blood with lower levels in the other tissues.

    Techniques Used: Selection, Isolation, In Vivo, Mouse Assay, Quantitative RT-PCR, Injection

    5) Product Images from "Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in"

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    Journal: BMC Molecular Biology

    doi: 10.1186/s12867-015-0039-3

    Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).
    Figure Legend Snippet: Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Techniques Used: RNA HS Assay, Concentration Assay

    Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.
    Figure Legend Snippet: Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Techniques Used: Standard Deviation

    6) Product Images from "Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry"

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry

    Journal: Current protocols in chemical biology

    doi: 10.1002/cpch.12

    Example of yields from the s 4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s 4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.
    Figure Legend Snippet: Example of yields from the s 4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s 4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.

    Techniques Used:

    7) Product Images from "How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?"

    Article Title: How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2016.12.021

    RNA profile of Mk and MkMP with or without RNase treatment (A) MkMPs were first treated with or without RNase I for 2 hours at 37 °C. After incubated with RNase inhibitors and washed with IMDM thrice, total RNA were isolated from MkMPs and RNA profiles were analyzed by Bioanalyzer 2100. (B) RNA profile of d12 Mks. (A) presents the data from two biological replicates to demonstrate the exquisite reproducibility of the RNA profiles before and after RNase treatment. The symbol (#) in (A) and (B) marks the range of small RNAs. 18S and 28S represent rRNA, while (δ) in (A) and (B) marks the ladder of 25 nt. (C) RNA profile of ladders for size verification. (D) Total RNA concentration from MkMPs treated with or without were measured by Qubit RNA HS kit. The measurement shows 65.5% of RNA being digested after RNA treatment. The data represent two biological replicates ± standard deviation.
    Figure Legend Snippet: RNA profile of Mk and MkMP with or without RNase treatment (A) MkMPs were first treated with or without RNase I for 2 hours at 37 °C. After incubated with RNase inhibitors and washed with IMDM thrice, total RNA were isolated from MkMPs and RNA profiles were analyzed by Bioanalyzer 2100. (B) RNA profile of d12 Mks. (A) presents the data from two biological replicates to demonstrate the exquisite reproducibility of the RNA profiles before and after RNase treatment. The symbol (#) in (A) and (B) marks the range of small RNAs. 18S and 28S represent rRNA, while (δ) in (A) and (B) marks the ladder of 25 nt. (C) RNA profile of ladders for size verification. (D) Total RNA concentration from MkMPs treated with or without were measured by Qubit RNA HS kit. The measurement shows 65.5% of RNA being digested after RNA treatment. The data represent two biological replicates ± standard deviation.

    Techniques Used: Incubation, Isolation, Concentration Assay, Standard Deviation

    8) Product Images from "Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in"

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    Journal: BMC Molecular Biology

    doi: 10.1186/s12867-015-0039-3

    Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).
    Figure Legend Snippet: Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Techniques Used: RNA HS Assay, Concentration Assay

    Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.
    Figure Legend Snippet: Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Techniques Used: Standard Deviation

    9) Product Images from "Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction"

    Article Title: Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction

    Journal: MethodsX

    doi: 10.1016/j.mex.2019.10.015

    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P
    Figure Legend Snippet: RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Techniques Used: Lysis, Spectrophotometry, RNA Extraction

    Related Articles

    RNA Extraction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    RNA HS Assay:

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
    Article Snippet: .. Validation of the Spike-in Qubit™ RNA HS assay The Qubit™ RNA HS Assay Kit (Life Technologies, Thermo Fisher Scientific Inc.), Qubit™ 2.0 Fluorometer (Life Technologies, Thermo Fisher Scientific Inc.) and Axygen PCR-05-C tubes (Axygen) were used for all measurements. ..

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes
    Article Snippet: .. RNA and DNA quantity and quality assessment Where indicated, RNA or DNA was quantified using Qubit RNA HS Assay Kit and Qubit dsDNA HS Assay Kit (Life Technologies), respectively, on the Qubit 2.0 Fluorometer (Life Technologies). .. In addition, where indicated, fragment size distribution of RNA or DNA samples was assessed on the Agilent 2100 BioAnalyzer using the Agilent RNA 6000 Pico Kit or Agilent High Sensitivity DNA Kit (Agilent Technologies), respectively.

    Article Title: Conserved roles for murine DUX and human DUX4 in activating cleavage stage genes and MERVL/HERVL retrotransposons
    Article Snippet: .. Diced siRNAs were purified using the PureLink™ Micro-to-Midi Total RNA purification Kit (Invitrogen, 12183-018) with modifications. siRNA concentration was measured with the Qubit® RNA HS Assay Kit (ThermoFisher, Q32852). mESCs containing the MERVL:GFP reporter were transfected with 20pmol (10pmol of each) of total siRNA using RNAiMax (Life Technologies). .. All siRNA transfections were performed twice (on back to back days) to ensure knockdown.

    Article Title: De novo dNTP production is essential for normal postnatal murine heart development
    Article Snippet: .. DNA and RNA concentrations were measured using the Qubit dsDNA HS and RNA HS assay kits, respectively, and a Qubit 2.0 fluorometer (Molecular Probes). .. DNA and RNA concentrations were measured using the Qubit dsDNA HS and RNA HS assay kits, respectively, and a Qubit 2.0 fluorometer (Molecular Probes).

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The concentration of this standard was measured using a Qubit RNA HS Assay Kit (Life Technologies, ), and a 10-fold dilution series was prepared in nuclease-free dH2 O supplemented with 100 ng/μL yeast tRNA. .. The dilution series samples were then processed in parallel with the synthetic nucleocapsid samples using the RNA purification and reverse transcription protocol above, and run on the same qPCR plate as the samples quantified.

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry
    Article Snippet: .. Baker, cat. no. 9344-13) MTSEA-biotin-XX (Biotium, cat. no. 89139-636) Chloroform: Isoamyl alcohol (Fisher Scientific, cat. no. 3160-450ML) Phase-lock gel tube, heavy 1.5 mL (5-Prime, cat. no. FP2302820) Glycogen (AmericanBio, cat. no AB00670-00020) Dynabeads MyOne Streptavidin C1 magnetic beads (Life Technologies, cat. no. 65001) Tris•HCl (Fisher Scientific, cat. no. T5941-500G) Sodium chloride (Sigma Aldrich, cat. no. S9888-2.5KG) Tween-20 (Mp Biomedicals Inc., cat. no. ICN19484180) β-mercaptoethanol (Sigma Aldrich, cat. no. M3148-25ML) Qubit RNA HS assay kit (Thermo Fisher Scientific, cat. no. ) Ambion century plus RNA marker (Thermo Fisher Scientific, cat. no. AM7145) MAXIscript T7 transcription kit (Thermo Fisher Scientific, cat. no. AM1312) Cy5-CTP (GE Healthcare, cat. no. 25-8010-87) 4-thiouridine-5’-triphosphate (s4 UTP) (Trilink Bio, cat. no. N-1025) SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics, cat. no. EC-829) Gel cassettes 1.0 mm (Life Sciences, cat. no. NC2010) Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000c) MagRack 6 magnetic stand (GE Healthcare, cat. no. 28948964) DynaMag 96 side (Life Technologies, cat. no. 12331D) Qubit 3.0 Fluorometer (Thermo Fisher Scientific, cat. no. ) Rotator (Glas-Col, cat. no. 099A MR1512) Typhoon FLA 9500 (GE Healthcare) .. In this section we describe the metabolic labeling of HEK293T RNA with s4 U.

    Magnetic Beads:

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry
    Article Snippet: .. Baker, cat. no. 9344-13) MTSEA-biotin-XX (Biotium, cat. no. 89139-636) Chloroform: Isoamyl alcohol (Fisher Scientific, cat. no. 3160-450ML) Phase-lock gel tube, heavy 1.5 mL (5-Prime, cat. no. FP2302820) Glycogen (AmericanBio, cat. no AB00670-00020) Dynabeads MyOne Streptavidin C1 magnetic beads (Life Technologies, cat. no. 65001) Tris•HCl (Fisher Scientific, cat. no. T5941-500G) Sodium chloride (Sigma Aldrich, cat. no. S9888-2.5KG) Tween-20 (Mp Biomedicals Inc., cat. no. ICN19484180) β-mercaptoethanol (Sigma Aldrich, cat. no. M3148-25ML) Qubit RNA HS assay kit (Thermo Fisher Scientific, cat. no. ) Ambion century plus RNA marker (Thermo Fisher Scientific, cat. no. AM7145) MAXIscript T7 transcription kit (Thermo Fisher Scientific, cat. no. AM1312) Cy5-CTP (GE Healthcare, cat. no. 25-8010-87) 4-thiouridine-5’-triphosphate (s4 UTP) (Trilink Bio, cat. no. N-1025) SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics, cat. no. EC-829) Gel cassettes 1.0 mm (Life Sciences, cat. no. NC2010) Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000c) MagRack 6 magnetic stand (GE Healthcare, cat. no. 28948964) DynaMag 96 side (Life Technologies, cat. no. 12331D) Qubit 3.0 Fluorometer (Thermo Fisher Scientific, cat. no. ) Rotator (Glas-Col, cat. no. 099A MR1512) Typhoon FLA 9500 (GE Healthcare) .. In this section we describe the metabolic labeling of HEK293T RNA with s4 U.

    Transfection:

    Article Title: Conserved roles for murine DUX and human DUX4 in activating cleavage stage genes and MERVL/HERVL retrotransposons
    Article Snippet: .. Diced siRNAs were purified using the PureLink™ Micro-to-Midi Total RNA purification Kit (Invitrogen, 12183-018) with modifications. siRNA concentration was measured with the Qubit® RNA HS Assay Kit (ThermoFisher, Q32852). mESCs containing the MERVL:GFP reporter were transfected with 20pmol (10pmol of each) of total siRNA using RNAiMax (Life Technologies). .. All siRNA transfections were performed twice (on back to back days) to ensure knockdown.

    Spectrophotometry:

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry
    Article Snippet: .. Baker, cat. no. 9344-13) MTSEA-biotin-XX (Biotium, cat. no. 89139-636) Chloroform: Isoamyl alcohol (Fisher Scientific, cat. no. 3160-450ML) Phase-lock gel tube, heavy 1.5 mL (5-Prime, cat. no. FP2302820) Glycogen (AmericanBio, cat. no AB00670-00020) Dynabeads MyOne Streptavidin C1 magnetic beads (Life Technologies, cat. no. 65001) Tris•HCl (Fisher Scientific, cat. no. T5941-500G) Sodium chloride (Sigma Aldrich, cat. no. S9888-2.5KG) Tween-20 (Mp Biomedicals Inc., cat. no. ICN19484180) β-mercaptoethanol (Sigma Aldrich, cat. no. M3148-25ML) Qubit RNA HS assay kit (Thermo Fisher Scientific, cat. no. ) Ambion century plus RNA marker (Thermo Fisher Scientific, cat. no. AM7145) MAXIscript T7 transcription kit (Thermo Fisher Scientific, cat. no. AM1312) Cy5-CTP (GE Healthcare, cat. no. 25-8010-87) 4-thiouridine-5’-triphosphate (s4 UTP) (Trilink Bio, cat. no. N-1025) SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics, cat. no. EC-829) Gel cassettes 1.0 mm (Life Sciences, cat. no. NC2010) Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000c) MagRack 6 magnetic stand (GE Healthcare, cat. no. 28948964) DynaMag 96 side (Life Technologies, cat. no. 12331D) Qubit 3.0 Fluorometer (Thermo Fisher Scientific, cat. no. ) Rotator (Glas-Col, cat. no. 099A MR1512) Typhoon FLA 9500 (GE Healthcare) .. In this section we describe the metabolic labeling of HEK293T RNA with s4 U.

    Purification:

    Article Title: Conserved roles for murine DUX and human DUX4 in activating cleavage stage genes and MERVL/HERVL retrotransposons
    Article Snippet: .. Diced siRNAs were purified using the PureLink™ Micro-to-Midi Total RNA purification Kit (Invitrogen, 12183-018) with modifications. siRNA concentration was measured with the Qubit® RNA HS Assay Kit (ThermoFisher, Q32852). mESCs containing the MERVL:GFP reporter were transfected with 20pmol (10pmol of each) of total siRNA using RNAiMax (Life Technologies). .. All siRNA transfections were performed twice (on back to back days) to ensure knockdown.

    Concentration Assay:

    Article Title: Conserved roles for murine DUX and human DUX4 in activating cleavage stage genes and MERVL/HERVL retrotransposons
    Article Snippet: .. Diced siRNAs were purified using the PureLink™ Micro-to-Midi Total RNA purification Kit (Invitrogen, 12183-018) with modifications. siRNA concentration was measured with the Qubit® RNA HS Assay Kit (ThermoFisher, Q32852). mESCs containing the MERVL:GFP reporter were transfected with 20pmol (10pmol of each) of total siRNA using RNAiMax (Life Technologies). .. All siRNA transfections were performed twice (on back to back days) to ensure knockdown.

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The concentration of this standard was measured using a Qubit RNA HS Assay Kit (Life Technologies, ), and a 10-fold dilution series was prepared in nuclease-free dH2 O supplemented with 100 ng/μL yeast tRNA. .. The dilution series samples were then processed in parallel with the synthetic nucleocapsid samples using the RNA purification and reverse transcription protocol above, and run on the same qPCR plate as the samples quantified.

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: .. Yield RNA concentration was determined using the Qubit HS RNA assay (Life Technologies, catalogue number Q32852) on the Qubit 2.0 fluorometer. ..

    Polymerase Chain Reaction:

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
    Article Snippet: .. Validation of the Spike-in Qubit™ RNA HS assay The Qubit™ RNA HS Assay Kit (Life Technologies, Thermo Fisher Scientific Inc.), Qubit™ 2.0 Fluorometer (Life Technologies, Thermo Fisher Scientific Inc.) and Axygen PCR-05-C tubes (Axygen) were used for all measurements. ..

    Marker:

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry
    Article Snippet: .. Baker, cat. no. 9344-13) MTSEA-biotin-XX (Biotium, cat. no. 89139-636) Chloroform: Isoamyl alcohol (Fisher Scientific, cat. no. 3160-450ML) Phase-lock gel tube, heavy 1.5 mL (5-Prime, cat. no. FP2302820) Glycogen (AmericanBio, cat. no AB00670-00020) Dynabeads MyOne Streptavidin C1 magnetic beads (Life Technologies, cat. no. 65001) Tris•HCl (Fisher Scientific, cat. no. T5941-500G) Sodium chloride (Sigma Aldrich, cat. no. S9888-2.5KG) Tween-20 (Mp Biomedicals Inc., cat. no. ICN19484180) β-mercaptoethanol (Sigma Aldrich, cat. no. M3148-25ML) Qubit RNA HS assay kit (Thermo Fisher Scientific, cat. no. ) Ambion century plus RNA marker (Thermo Fisher Scientific, cat. no. AM7145) MAXIscript T7 transcription kit (Thermo Fisher Scientific, cat. no. AM1312) Cy5-CTP (GE Healthcare, cat. no. 25-8010-87) 4-thiouridine-5’-triphosphate (s4 UTP) (Trilink Bio, cat. no. N-1025) SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics, cat. no. EC-829) Gel cassettes 1.0 mm (Life Sciences, cat. no. NC2010) Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000c) MagRack 6 magnetic stand (GE Healthcare, cat. no. 28948964) DynaMag 96 side (Life Technologies, cat. no. 12331D) Qubit 3.0 Fluorometer (Thermo Fisher Scientific, cat. no. ) Rotator (Glas-Col, cat. no. 099A MR1512) Typhoon FLA 9500 (GE Healthcare) .. In this section we describe the metabolic labeling of HEK293T RNA with s4 U.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher qubit rna hs assay kit
    Example of yields from the s 4 <t>U-RNA</t> enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s 4 U was enriched using the main text protocol and assayed by <t>Qubit.</t> Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.
    Qubit Rna Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit rna hs assay kit/product/Thermo Fisher
    Average 99 stars, based on 446 article reviews
    Price from $9.99 to $1999.99
    qubit rna hs assay kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Example of yields from the s 4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s 4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.

    Journal: Current protocols in chemical biology

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry

    doi: 10.1002/cpch.12

    Figure Lengend Snippet: Example of yields from the s 4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s 4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.

    Article Snippet: Baker, cat. no. 9344-13) MTSEA-biotin-XX (Biotium, cat. no. 89139-636) Chloroform: Isoamyl alcohol (Fisher Scientific, cat. no. 3160-450ML) Phase-lock gel tube, heavy 1.5 mL (5-Prime, cat. no. FP2302820) Glycogen (AmericanBio, cat. no AB00670-00020) Dynabeads MyOne Streptavidin C1 magnetic beads (Life Technologies, cat. no. 65001) Tris•HCl (Fisher Scientific, cat. no. T5941-500G) Sodium chloride (Sigma Aldrich, cat. no. S9888-2.5KG) Tween-20 (Mp Biomedicals Inc., cat. no. ICN19484180) β-mercaptoethanol (Sigma Aldrich, cat. no. M3148-25ML) Qubit RNA HS assay kit (Thermo Fisher Scientific, cat. no. ) Ambion century plus RNA marker (Thermo Fisher Scientific, cat. no. AM7145) MAXIscript T7 transcription kit (Thermo Fisher Scientific, cat. no. AM1312) Cy5-CTP (GE Healthcare, cat. no. 25-8010-87) 4-thiouridine-5’-triphosphate (s4 UTP) (Trilink Bio, cat. no. N-1025) SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics, cat. no. EC-829) Gel cassettes 1.0 mm (Life Sciences, cat. no. NC2010) Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000c) MagRack 6 magnetic stand (GE Healthcare, cat. no. 28948964) DynaMag 96 side (Life Technologies, cat. no. 12331D) Qubit 3.0 Fluorometer (Thermo Fisher Scientific, cat. no. ) Rotator (Glas-Col, cat. no. 099A MR1512) Typhoon FLA 9500 (GE Healthcare)

    Techniques:

    Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Journal: BMC Molecular Biology

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    doi: 10.1186/s12867-015-0039-3

    Figure Lengend Snippet: Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Article Snippet: “RNA spike-in” was made by diluting the Qubit™ RNA Standard #2 (10 ng/μL rRNA) included in the Qubit™ RNA HS Assay kit to 2.5 ng/μL and 2 μL was added into each tube.

    Techniques: RNA HS Assay, Concentration Assay

    Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Journal: BMC Molecular Biology

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    doi: 10.1186/s12867-015-0039-3

    Figure Lengend Snippet: Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Article Snippet: “RNA spike-in” was made by diluting the Qubit™ RNA Standard #2 (10 ng/μL rRNA) included in the Qubit™ RNA HS Assay kit to 2.5 ng/μL and 2 μL was added into each tube.

    Techniques: Standard Deviation

    RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Journal: MethodsX

    Article Title: Inappropriateness of RNAlater to preserve Caenorhabditis elegans for RNA extraction

    doi: 10.1016/j.mex.2019.10.015

    Figure Lengend Snippet: RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R 2 = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P

    Article Snippet: Qubit RNA HS Assay kit (Thermo Fisher Scientific, Cat.#Q32852) and Qubit® 2.0 Fluorometer (Invitrogen) were used to determine the level of RNA content in the worm lysates according to instructions from the manufacturer.

    Techniques: Lysis, Spectrophotometry, RNA Extraction