qubit rna hs assay kit  (Thermo Fisher)


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    Name:
    Qubit RNA HS Assay Kit
    Description:
    The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer.Which product to choose for RNA HS (High Sensitivity) quantitation? • For 1–20 samples: use this Qubit RNA HS Assay Kit with the Qubit Fluorometer • For 20–2000 samples: use the Quant-iT RNA HS Assay Kit with microplate readerNotes:1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.2. 500 µL thin-walled PCR tubes are required but not included.
    Catalog Number:
    Q32852
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|RNA Quantitation|Nucleic Acid Quantitation
    Size:
    100 assays
    Category:
    Kits and Assays, DNA⁄RNA Quantitation Assay Kits
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher qubit rna hs assay kit
    Example of yields from the s4 <t>U-RNA</t> enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s4 U was enriched using the main text protocol and assayed by <t>Qubit.</t> Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.
    The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer.Which product to choose for RNA HS (High Sensitivity) quantitation? • For 1–20 samples: use this Qubit RNA HS Assay Kit with the Qubit Fluorometer • For 20–2000 samples: use the Quant-iT RNA HS Assay Kit with microplate readerNotes:1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.2. 500 µL thin-walled PCR tubes are required but not included.
    https://www.bioz.com/result/qubit rna hs assay kit/product/Thermo Fisher
    Average 99 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    qubit rna hs assay kit - by Bioz Stars, 2019-12
    99/100 stars

    Images

    1) Product Images from "Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry"

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry

    Journal:

    doi: 10.1002/cpch.12

    Example of yields from the s4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.
    Figure Legend Snippet: Example of yields from the s4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.

    Techniques Used:

    2) Product Images from "Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in"

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    Journal: BMC Molecular Biology

    doi: 10.1186/s12867-015-0039-3

    Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).
    Figure Legend Snippet: Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Techniques Used: RNA HS Assay, Concentration Assay

    Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.
    Figure Legend Snippet: Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Techniques Used: Standard Deviation

    3) Product Images from "Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in"

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    Journal: BMC Molecular Biology

    doi: 10.1186/s12867-015-0039-3

    Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).
    Figure Legend Snippet: Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Techniques Used: RNA HS Assay, Concentration Assay

    Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.
    Figure Legend Snippet: Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Techniques Used: Standard Deviation

    4) Product Images from "Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine"

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine

    Journal: BMC Cancer

    doi: 10.1186/s12885-016-2930-9

    a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5 × 10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls
    Figure Legend Snippet: a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5 × 10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Techniques Used: RNA Extraction, Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction

    5) Product Images from "Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?"

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

    Journal: BMC Clinical Pathology

    doi: 10.1186/s12907-016-0039-3

    Comparison of Qubit RNA yields obtained using four off the shelf extraction kits: Geometric mean RNA yield and associated geometric standard error of mean for the four extraction kits tested in this study, n = 30 for each method. P-values show the statistical significance of the difference in yield between each method
    Figure Legend Snippet: Comparison of Qubit RNA yields obtained using four off the shelf extraction kits: Geometric mean RNA yield and associated geometric standard error of mean for the four extraction kits tested in this study, n = 30 for each method. P-values show the statistical significance of the difference in yield between each method

    Techniques Used:

    6) Product Images from "Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes"

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

    Journal: Scientific Reports

    doi: 10.1038/srep41114

    Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.
    Figure Legend Snippet: Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

    Techniques Used: Isolation, Quantitation Assay, RNA HS Assay, Software, Fluorescence

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    Article Snippet: The lysate was clarified by centrifugation for 10 min at 15,000 x g at 4°C in a tabletop microcentrifuge. .. Total RNA in the lysate was quantified using the Qubit RNA HS Assay Kit (Thermo Fisher).

    Amplification:

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    Synthesized:

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    Construct:

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    Real-time Polymerase Chain Reaction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. All amplification reactions were prepared with Q5® High-Fidelity PCR Kit (New England BioLabs, Cat # E0555S).

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    Article Snippet: RNA was quantified using the Qubit® RNA HS Assay Kit (Life Technologies). .. 200 ng RNA was used to synthesize cDNA using the Invitrogen SuperScript® III First-Strand Synthesis System for RT-PCR (Life Technologies). qPCR was performed in 384-well plates using SYBR® Green dye.

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: The RNASeq libraries were then prepared using Clontech SMARTer® Stranded Total RNA-Seq Kit (Cat # 635006) and purified using AMPure beads (Beckman coulter). .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR. .. The quality of the library was determined using Bioanalyzer (Agilent).

    Article Title: Jagged1 protein processing in the developing mammalian lens
    Article Snippet: Paragraph title: RNA purification and quantitative PCR analysis ... RNA concentrations were measured with a Qubit 3.0 Fluorometer and Molecular Probes Qubit RNA HS Assay kit (Cat#:Q32852).

    Incubation:

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    Article Snippet: After washing, purified protein complexes were incubated with total RNA for 30 min at RT. .. RNA was treated with or without RNase A (Thermo Fisher #EN0531) and quantified using Qubit® High Sensitive Assay kit (Life Technologies #Q32852).

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    Article Snippet: To analyze recovery from emetine, cells treated with low dose emetine were washed twice with emetine-free media, harvested immediately (no recovery) or incubated with media lacking emetine for 1 hr and transferred to ice. .. Total RNA in the lysate was quantified using the Qubit RNA HS Assay Kit (Thermo Fisher).

    Formalin-fixed Paraffin-Embedded:

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    Article Snippet: Paragraph title: RNA recovery from FFPE specimens ... RNA was quantified using the Qubit® RNA HS Assay Kit (Life Technologies).

    RNA Binding Assay:

    Article Title: RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells
    Article Snippet: Paragraph title: In vitro RNA-binding assay (iv-RIP) ... RNA was treated with or without RNase A (Thermo Fisher #EN0531) and quantified using Qubit® High Sensitive Assay kit (Life Technologies #Q32852).

    Fluorescence:

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    Two Tailed Test:

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    Concentration Assay:

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    Protease Inhibitor:

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    Genomic Sequencing:

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    Dissection:

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    Generated:

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    DNA HS Assay:

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    DNA Sequencing:

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    Article Snippet: The Agilent High Sensitivity DNA Kit with the Agilent 2100 Bioanalyzer (Life Technologies, Waldbronn, Germany) was used to size, quantify and quality control DNA sequencing libraries. .. RNA concentration was measured using the Qubit RNA HS Assay kit (Life Technologies).

    Reverse Transcription Polymerase Chain Reaction:

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    DNA Extraction:

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    Article Snippet: The powder mycelium was re-suspended in 0.8 mL DNA extraction buffer (0.1 M Tris-HCl, pH 8.0, 1.2 M NaCl, and 5 mM EDTA) and mixed by vortexing. .. The concentration was determined using the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific, MA, United States).

    Article Title: Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory
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    Nucleic Acid Electrophoresis:

    Article Title: Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory
    Article Snippet: Reactions were cycled with the following conditions: 95 ° C for 5 min followed by 35 cycles of 95 ° C for 30 s, 51 ° C for 30 s and 72 ° C for 1 min, then a final 10 min extension at 72 ° C. Reactions were analyzed via gel electrophoresis to confirm that all exposed animals had come into contact with E. muscae 'Berkeley' and that control animals were uninfected. .. RNA was quantified using a Qubit Fluorometer (Qubit RNA HS assay kit, ThermoFisher Scientific) and quality was checked by running on a RNA 6000 Pico chip on a Bioanalyzer 2100 (Agilent Technologies).

    RNA Sequencing Assay:

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: The RNASeq libraries were then prepared using Clontech SMARTer® Stranded Total RNA-Seq Kit (Cat # 635006) and purified using AMPure beads (Beckman coulter). .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    Article Title: Comparison of Transcriptomic Platforms for Analysis of Whole Blood from Ebola-Infected Cynomolgus Macaques
    Article Snippet: For RNA-Seq analyses, RNA was isolated from whole blood in TRIzol LS using the PureLink RNA Mini Kit (ThermoFisher Scientific) and RNA was evaluated for quality on the Agilent 2200 TapeStation. .. For NanoString analyses, total RNA was isolated using the miRNeasy Mini Kit (Qiagen) and then quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific).

    Article Title: Transcriptome-wide analysis of a baculovirus using nanopore sequencing
    Article Snippet: For the cDNA and Direct RNA sequencing, the polyA(+) fraction of the RNA samples were purified using the Qiagen Oligotex mRNA Mini Kit, according to the “Spin Columns” protocol of the kit. .. The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined with Qubit RNA HS Assay Kit (Life Technologies).

    Article Title: A method to identify respiratory virus infections in clinical samples using next-generation sequencing
    Article Snippet: The library was processed for NGS sample preparation using SMARTer Stranded RNA-Seq Kits (Clontech, a Takara Bio Company, CA, USA), Strand-Specific Library Construction for Transcriptome Analysis on Illumina Platforms. .. RNA concentration was measured using the Qubit RNA HS Assay kit (Life Technologies).

    RNA HS Assay:

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
    Article Snippet: We named the modified assay the Spike-in Qubit™ RNA HS Assay because this optimization takes advantage of an RNA spike-in. .. The Qubit™ RNA HS Assay Kit (Life Technologies, Thermo Fisher Scientific Inc.), Qubit™ 2.0 Fluorometer (Life Technologies, Thermo Fisher Scientific Inc.) and Axygen PCR-05-C tubes (Axygen) were used for all measurements. .. The Qubit™ working solution was made according to manufacturer’s instructions.

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
    Article Snippet: 10 μL of the Qubit™ RNA Standard solutions were used for standard tubes. .. “RNA spike-in” was made by diluting the Qubit™ RNA Standard #2 (10 ng/μL rRNA) included in the Qubit™ RNA HS Assay kit to 2.5 ng/μL and 2 μL was added into each tube. .. 18 μL of water was added into one tube for RNA spike-in alone reading.

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes
    Article Snippet: RNA quantity and quality were assessed as described below. .. Where indicated, RNA or DNA was quantified using Qubit RNA HS Assay Kit and Qubit dsDNA HS Assay Kit (Life Technologies), respectively, on the Qubit 2.0 Fluorometer (Life Technologies). .. In addition, where indicated, fragment size distribution of RNA or DNA samples was assessed on the Agilent 2100 BioAnalyzer using the Agilent RNA 6000 Pico Kit or Agilent High Sensitivity DNA Kit (Agilent Technologies), respectively.

    Article Title: Organocatalytic Removal of Formaldehyde Adducts from RNA and DNA Bases
    Article Snippet: Catalyst 3 was incorporated into the AllPrep® protocol by addition of an aqueous solution of 3 at pH 7 to the RNA-containing lysate. .. RNA was quantified using the Qubit® RNA HS Assay Kit (Life Technologies). .. 200 ng RNA was used to synthesize cDNA using the Invitrogen SuperScript® III First-Strand Synthesis System for RT-PCR (Life Technologies). qPCR was performed in 384-well plates using SYBR® Green dye.

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: The RNASeq libraries were then prepared using Clontech SMARTer® Stranded Total RNA-Seq Kit (Cat # 635006) and purified using AMPure beads (Beckman coulter). .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR. .. The quality of the library was determined using Bioanalyzer (Agilent).

    Article Title: Jagged1 protein processing in the developing mammalian lens
    Article Snippet: Total B3 cell RNA was isolated using the Zymo Research Quick RNA miniprep kit (Cat#:R1055). .. RNA concentrations were measured with a Qubit 3.0 Fluorometer and Molecular Probes Qubit RNA HS Assay kit (Cat#:Q32852). .. 100 ng of total RNA was reverse transcribed into cDNA via the Bio-Rad iScript cDNA Synthesis kit (Cat#:170-8891) and used for qPCR analysis using Applied BioSystems Fast Sybr Green Master Mix (Cat#:4385614) and primer sets listed in Table S1 on an Applied Biosystems StepOnePlus machine.

    Article Title: Comparison of Transcriptomic Platforms for Analysis of Whole Blood from Ebola-Infected Cynomolgus Macaques
    Article Snippet: For RNA-Seq analyses, RNA was isolated from whole blood in TRIzol LS using the PureLink RNA Mini Kit (ThermoFisher Scientific) and RNA was evaluated for quality on the Agilent 2200 TapeStation. .. For NanoString analyses, total RNA was isolated using the miRNeasy Mini Kit (Qiagen) and then quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific). .. Libraries were generated on the Sciclone G3 Liquid Handling Robot (PerkinElmer) using the TruSeq Stranded Total RNA Library Prep Kit (Illumina).

    Article Title: Forward Genetics by Genome Sequencing Uncovers the Central Role of the Aspergillus niger goxB Locus in Hydrogen Peroxide Induced Glucose Oxidase Expression
    Article Snippet: Genomic DNA was re-suspended in 50 μL of milliQ water. .. The concentration was determined using the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific, MA, United States). .. Whole genome sequencing of strain N402 was performed by a commercial vendor (Novogene Bioinformatics Technology Co. Ltd, Beijing, China.) using Illumina HiSeq (300 bp inserts library with 150 bp paired-end sequencing; Illumina, San Diego, CA, United States) and PacBio RS II platform (10 kb inserts library; Pacific Biosciences, Menlo Park, CA, United States).

    Article Title: ZNF598 Is a Quality Control Sensor of Collided Ribosomes
    Article Snippet: The lysate was clarified by centrifugation for 10 min at 15,000 x g at 4°C in a tabletop microcentrifuge. .. Total RNA in the lysate was quantified using the Qubit RNA HS Assay Kit (Thermo Fisher). .. Lysate containing 90 μg of total RNA was adjusted to 1 mM CaCl2 and 0.5 U of S7 nuclease per μg of RNA in a total reaction volume of 300 μL.

    Article Title: YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
    Article Snippet: Total RNA was extracted using the Macaloid Clay method outlined by Raya, et al . .. The concentration of RNA was quantified by using the Qubit® RNA HS Assay Kit (Life Technologies, Buenos Aires, Argentina). .. The DNA was removed with 5 U of TURBO™ DNase (Life Technologies).

    Article Title: Transcriptome-wide analysis of a baculovirus using nanopore sequencing
    Article Snippet: For the cDNA and Direct RNA sequencing, the polyA(+) fraction of the RNA samples were purified using the Qiagen Oligotex mRNA Mini Kit, according to the “Spin Columns” protocol of the kit. .. The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined with Qubit RNA HS Assay Kit (Life Technologies). .. The Oxford Nanopore Technologies MinION real-time device was used for cDNA and direct RNA sequencing.

    Article Title: Transcriptomic study of Herpes simplex virus type-1 using full-length sequencing techniques
    Article Snippet: For the sequencing of the polyadenylated [polyA(+)] fraction of the samples, RNAs were purified by using the Qiagen Oligotex mRNA Mini Kit, following to the “Spin Columns” protocol of the kit. .. The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined through use of the Qubit RNA HS Assay Kit (Life Technologies). .. Full-length cDNAs were prepared according to the PacBio Isoform Sequencing (Iso-Seq) protocol by using the Clontech SMARTer PCR cDNA Synthesis Kit.

    Article Title: Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory
    Article Snippet: RNA was prepared from each thawed sample by homogenizing with an RNase-free pestle (Kimble Chase), washing the pestle with 750 µ L Trizol, then proceeding using the manufacturer’s protocol. .. RNA was quantified using a Qubit Fluorometer (Qubit RNA HS assay kit, ThermoFisher Scientific) and quality was checked by running on a RNA 6000 Pico chip on a Bioanalyzer 2100 (Agilent Technologies). .. One replicate control RNA sample for the 48 hr time point was lost prior to library preparation so was omitted.

    Article Title: A method to identify respiratory virus infections in clinical samples using next-generation sequencing
    Article Snippet: For the detection of RNA viruses, sample extracts were pretreated with RNase free DNase (promega), the Agilent RNA 6000 Nano Kit (Life Technologies, Waldbronn, Germany) was used to evaluate total RNA quality. .. RNA concentration was measured using the Qubit RNA HS Assay kit (Life Technologies). .. RNA libraries were constructed for NGS using SMARTer Stranded RNA-Seq Kit according to manufacturer instructions (Clontech, a Takara Bio Company, CA, USA).

    Article Title: Mass Azithromycin Distribution and Community Microbiome: A Cluster-Randomized Trial
    Article Snippet: RNA was extracted from 300 fecal samples using the Norgen Stool RNA Isolation Kit (Norgen, Ontario, Canada), per the manufacturer’s instructions. .. The RNA concentration of each sample was quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA) and normalized for pooling. .. The RNA from the 300 samples was pooled into 30 samples (10 samples per village).

    Sensitive Assay:

    Article Title: RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells
    Article Snippet: Beads containing protein-RNA complexes were then washed and eluted in TRIzol and immunoprecipitated RNA was purified. .. RNA was treated with or without RNase A (Thermo Fisher #EN0531) and quantified using Qubit® High Sensitive Assay kit (Life Technologies #Q32852). .. Immunoprecipitated RNA was visualized in an ethidium bromide agarose gel and retrotranscribed with qSCRIPT (Quanta #95048) to be analyzed by qPCR.

    Isolation:

    Article Title: Organocatalytic Removal of Formaldehyde Adducts from RNA and DNA Bases
    Article Snippet: RNA was quantified using the Qubit® RNA HS Assay Kit (Life Technologies). .. 200 ng RNA was used to synthesize cDNA using the Invitrogen SuperScript® III First-Strand Synthesis System for RT-PCR (Life Technologies). qPCR was performed in 384-well plates using SYBR® Green dye.

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: Collected tissues were lysed in extraction buffer and total RNA was isolated using PicoPure RNA isolation kit (ThermoFisher). .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    Article Title: Jagged1 protein processing in the developing mammalian lens
    Article Snippet: Total B3 cell RNA was isolated using the Zymo Research Quick RNA miniprep kit (Cat#:R1055). .. RNA concentrations were measured with a Qubit 3.0 Fluorometer and Molecular Probes Qubit RNA HS Assay kit (Cat#:Q32852).

    Article Title: Comparison of Transcriptomic Platforms for Analysis of Whole Blood from Ebola-Infected Cynomolgus Macaques
    Article Snippet: For RNA-Seq analyses, RNA was isolated from whole blood in TRIzol LS using the PureLink RNA Mini Kit (ThermoFisher Scientific) and RNA was evaluated for quality on the Agilent 2200 TapeStation. .. For NanoString analyses, total RNA was isolated using the miRNeasy Mini Kit (Qiagen) and then quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific). .. Libraries were generated on the Sciclone G3 Liquid Handling Robot (PerkinElmer) using the TruSeq Stranded Total RNA Library Prep Kit (Illumina).

    Article Title: Forward Genetics by Genome Sequencing Uncovers the Central Role of the Aspergillus niger goxB Locus in Hydrogen Peroxide Induced Glucose Oxidase Expression
    Article Snippet: Paragraph title: Isolation of Genomic DNA of A. niger for Whole Genome Sequencing ... The concentration was determined using the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific, MA, United States).

    Article Title: YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... The concentration of RNA was quantified by using the Qubit® RNA HS Assay Kit (Life Technologies, Buenos Aires, Argentina).

    Article Title: Transcriptome-wide analysis of a baculovirus using nanopore sequencing
    Article Snippet: Paragraph title: Isolation of polyadenylated RNA ... The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined with Qubit RNA HS Assay Kit (Life Technologies).

    Article Title: Transcriptomic study of Herpes simplex virus type-1 using full-length sequencing techniques
    Article Snippet: Paragraph title: Isolation of polyadenylated RNA ... The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined through use of the Qubit RNA HS Assay Kit (Life Technologies).

    Article Title: Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory
    Article Snippet: The body of each animal was saved and subjected to a DNA extraction using the manufacturer’s provided protocol for the isolation of genomic DNA from tissues (QIAamp DNA Micro kit, QIAGEN) eluting in 20 µL of buffer AE. .. RNA was quantified using a Qubit Fluorometer (Qubit RNA HS assay kit, ThermoFisher Scientific) and quality was checked by running on a RNA 6000 Pico chip on a Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Mass Azithromycin Distribution and Community Microbiome: A Cluster-Randomized Trial
    Article Snippet: RNA was extracted from 300 fecal samples using the Norgen Stool RNA Isolation Kit (Norgen, Ontario, Canada), per the manufacturer’s instructions. .. The RNA concentration of each sample was quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA) and normalized for pooling.

    Polymerase Chain Reaction:

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
    Article Snippet: We named the modified assay the Spike-in Qubit™ RNA HS Assay because this optimization takes advantage of an RNA spike-in. .. The Qubit™ RNA HS Assay Kit (Life Technologies, Thermo Fisher Scientific Inc.), Qubit™ 2.0 Fluorometer (Life Technologies, Thermo Fisher Scientific Inc.) and Axygen PCR-05-C tubes (Axygen) were used for all measurements. .. The Qubit™ working solution was made according to manufacturer’s instructions.

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852).

    Article Title: YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
    Article Snippet: The concentration of RNA was quantified by using the Qubit® RNA HS Assay Kit (Life Technologies, Buenos Aires, Argentina). .. The concentration of RNA was quantified by using the Qubit® RNA HS Assay Kit (Life Technologies, Buenos Aires, Argentina).

    Article Title: Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory
    Article Snippet: For each fly body, 1 µL was used to template a PCR reaction consisting of 12.5 µL GoTaq, 2 µL of each primer emITS1 and emITS4 (10 µM stocks), and 7.5 µL water for a final volume of 25 µL. .. RNA was quantified using a Qubit Fluorometer (Qubit RNA HS assay kit, ThermoFisher Scientific) and quality was checked by running on a RNA 6000 Pico chip on a Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Mass Azithromycin Distribution and Community Microbiome: A Cluster-Randomized Trial
    Article Snippet: The RNA concentration of each sample was quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA) and normalized for pooling. .. The RNA concentration of each sample was quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA) and normalized for pooling.

    Microscopy:

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: VMHdm, VMHavl, VMHpvlm and VMHpvll was laser-dissected using LMD6000 (Leica; a fluorescent microscope combined with a movable laser for microdissection). .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    Purification:

    Article Title: RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells
    Article Snippet: Beads containing protein-RNA complexes were then washed and eluted in TRIzol and immunoprecipitated RNA was purified. .. RNA was treated with or without RNase A (Thermo Fisher #EN0531) and quantified using Qubit® High Sensitive Assay kit (Life Technologies #Q32852).

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: The RNASeq libraries were then prepared using Clontech SMARTer® Stranded Total RNA-Seq Kit (Cat # 635006) and purified using AMPure beads (Beckman coulter). .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    Article Title: Jagged1 protein processing in the developing mammalian lens
    Article Snippet: Paragraph title: RNA purification and quantitative PCR analysis ... RNA concentrations were measured with a Qubit 3.0 Fluorometer and Molecular Probes Qubit RNA HS Assay kit (Cat#:Q32852).

    Article Title: Transcriptome-wide analysis of a baculovirus using nanopore sequencing
    Article Snippet: For the cDNA and Direct RNA sequencing, the polyA(+) fraction of the RNA samples were purified using the Qiagen Oligotex mRNA Mini Kit, according to the “Spin Columns” protocol of the kit. .. The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined with Qubit RNA HS Assay Kit (Life Technologies).

    Article Title: Transcriptomic study of Herpes simplex virus type-1 using full-length sequencing techniques
    Article Snippet: For the sequencing of the polyadenylated [polyA(+)] fraction of the samples, RNAs were purified by using the Qiagen Oligotex mRNA Mini Kit, following to the “Spin Columns” protocol of the kit. .. The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined through use of the Qubit RNA HS Assay Kit (Life Technologies).

    Sequencing:

    Article Title: Forward Genetics by Genome Sequencing Uncovers the Central Role of the Aspergillus niger goxB Locus in Hydrogen Peroxide Induced Glucose Oxidase Expression
    Article Snippet: Paragraph title: Isolation of Genomic DNA of A. niger for Whole Genome Sequencing ... The concentration was determined using the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific, MA, United States).

    Article Title: Transcriptomic study of Herpes simplex virus type-1 using full-length sequencing techniques
    Article Snippet: For the sequencing of the polyadenylated [polyA(+)] fraction of the samples, RNAs were purified by using the Qiagen Oligotex mRNA Mini Kit, following to the “Spin Columns” protocol of the kit. .. The final concentrations of the rRNA depleted and the PolyA(+) RNA samples were determined through use of the Qubit RNA HS Assay Kit (Life Technologies).

    Article Title: A method to identify respiratory virus infections in clinical samples using next-generation sequencing
    Article Snippet: Paragraph title: Library preparation and sequencing ... RNA concentration was measured using the Qubit RNA HS Assay kit (Life Technologies).

    Article Title: Mass Azithromycin Distribution and Community Microbiome: A Cluster-Randomized Trial
    Article Snippet: Paragraph title: Sample Processing and Sequencing ... The RNA concentration of each sample was quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA) and normalized for pooling.

    Labeling:

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: Given that VMH is composed of largely glutamatergic cells that were surrounded by GABAergic cells and each subdivision of VMH has cell-poor boundary, the ZsGreen labeling in glutamatergic cells enabled recognition of the subdivisions. .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    Agarose Gel Electrophoresis:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Jagged1 protein processing in the developing mammalian lens
    Article Snippet: Total B3 cell RNA was isolated using the Zymo Research Quick RNA miniprep kit (Cat#:R1055). .. RNA concentrations were measured with a Qubit 3.0 Fluorometer and Molecular Probes Qubit RNA HS Assay kit (Cat:Q32852). .. 100 ng of total RNA was reverse transcribed into cDNA via the Bio-Rad iScript cDNA Synthesis kit (Cat#:170-8891) and used for qPCR analysis using Applied BioSystems Fast Sybr Green Master Mix (Cat#:4385614) and primer sets listed in Table S1 on an Applied Biosystems StepOnePlus machine.

    Chromatin Immunoprecipitation:

    Article Title: Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory
    Article Snippet: RNA was prepared from each thawed sample by homogenizing with an RNase-free pestle (Kimble Chase), washing the pestle with 750 µ L Trizol, then proceeding using the manufacturer’s protocol. .. RNA was quantified using a Qubit Fluorometer (Qubit RNA HS assay kit, ThermoFisher Scientific) and quality was checked by running on a RNA 6000 Pico chip on a Bioanalyzer 2100 (Agilent Technologies). .. One replicate control RNA sample for the 48 hr time point was lost prior to library preparation so was omitted.

    Software:

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR. .. The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    RNA Extraction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: Surviving cells were evidenced following fixation in 90% ethanol and cell staining with 0.5% crystal violet (Cat # C-3886, Sigma–Aldrich, St. Louis, MO. .. Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Sample Prep:

    Article Title: A method to identify respiratory virus infections in clinical samples using next-generation sequencing
    Article Snippet: The library was processed for NGS sample preparation using SMARTer Stranded RNA-Seq Kits (Clontech, a Takara Bio Company, CA, USA), Strand-Specific Library Construction for Transcriptome Analysis on Illumina Platforms. .. RNA concentration was measured using the Qubit RNA HS Assay kit (Life Technologies).

    In Vitro:

    Article Title: RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells
    Article Snippet: Paragraph title: In vitro RNA-binding assay (iv-RIP) ... RNA was treated with or without RNase A (Thermo Fisher #EN0531) and quantified using Qubit® High Sensitive Assay kit (Life Technologies #Q32852).

    Next-Generation Sequencing:

    Article Title: A method to identify respiratory virus infections in clinical samples using next-generation sequencing
    Article Snippet: NGS was performed using a MiSeq v2 kit (500 cycles) (Illumina, San Diego, CA). .. RNA concentration was measured using the Qubit RNA HS Assay kit (Life Technologies).

    Laser Capture Microdissection:

    Article Title: Esr1+ cells in the ventromedial hypothalamus control female aggression
    Article Snippet: Paragraph title: LCM-RNAseq ... The quantity of the library was examined using Qubit RNA HS assay Kit (Thermo Fisher Scientific) and qPCR.

    Random Hexamer Labeling:

    Article Title: YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
    Article Snippet: The concentration of RNA was quantified by using the Qubit® RNA HS Assay Kit (Life Technologies, Buenos Aires, Argentina). .. The concentration of RNA was quantified by using the Qubit® RNA HS Assay Kit (Life Technologies, Buenos Aires, Argentina).

    Immunoprecipitation:

    Article Title: RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells
    Article Snippet: Beads containing protein-RNA complexes were then washed and eluted in TRIzol and immunoprecipitated RNA was purified. .. RNA was treated with or without RNase A (Thermo Fisher #EN0531) and quantified using Qubit® High Sensitive Assay kit (Life Technologies #Q32852).

    Lysis:

    Article Title: ZNF598 Is a Quality Control Sensor of Collided Ribosomes
    Article Snippet: Lysis was in 300 μL of buffer containing 20 mM HEPES, pH 7.4, 100 mM KOAc, 5 mM Mg(OAc)2 , 0.5% Triton X-100, 1 mM DTT, and 1X EDTA-free protease inhibitor cocktail (Roche) for 15 min on ice. .. Total RNA in the lysate was quantified using the Qubit RNA HS Assay Kit (Thermo Fisher).

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    Thermo Fisher qubit rna hs assay kit
    Schematic diagram of the Spike-in <t>Qubit™</t> <t>RNA</t> HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).
    Qubit Rna Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit rna hs assay kit/product/Thermo Fisher
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    Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Journal: BMC Molecular Biology

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    doi: 10.1186/s12867-015-0039-3

    Figure Lengend Snippet: Schematic diagram of the Spike-in Qubit™ RNA HS Assay. RNA spike-in (green) was added to reach the lower quantification limit of Qubit™. Then nuclease-free water or RNA sample (orange) was added for Qubit™ measurement. R1 is the reading for RNA spike-in alone and R2 for RNA spike-in plus RNA sample. The reading for RNA sample is (R2 - R1) and RNA sample concentration is calculated as [sample] = (R2 – R1) (pg/μL) × assay volume (μL) ÷ sample volume for the assay (μL).

    Article Snippet: “RNA spike-in” was made by diluting the Qubit™ RNA Standard #2 (10 ng/μL rRNA) included in the Qubit™ RNA HS Assay kit to 2.5 ng/μL and 2 μL was added into each tube.

    Techniques: RNA HS Assay, Concentration Assay

    Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Journal: BMC Molecular Biology

    Article Title: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

    doi: 10.1186/s12867-015-0039-3

    Figure Lengend Snippet: Reading increases between 1 and 20 pg/μL show a strong linear correlation in the Spike-in Qubit™ RNA Assay. RNA spike-in alone or with increasing amounts of a 250 pg/μL Qubit™ RNA Standard #2 sample (A) or a 250 pg/μL trophoblast total RNA sample (B) was measured by the Qubit™ Assay. Reading increases over RNA spike-in were plotted against expected reading increases. Regression line equation, coefficient of determination (R 2 ) and error bars indicating standard deviation are shown. N = 4 independent repeats.

    Article Snippet: “RNA spike-in” was made by diluting the Qubit™ RNA Standard #2 (10 ng/μL rRNA) included in the Qubit™ RNA HS Assay kit to 2.5 ng/μL and 2 μL was added into each tube.

    Techniques: Standard Deviation

    Example of yields from the s4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.

    Journal:

    Article Title: Enriching s4U-RNA using methane thiosulfonate (MTS) chemistry

    doi: 10.1002/cpch.12

    Figure Lengend Snippet: Example of yields from the s4 U-RNA enrichment described in this protocol. RNA from cells treated for 2h or 30 min with s4 U was enriched using the main text protocol and assayed by Qubit. Percent input was calculated for enriched samples relative to 10% input standards for each sample. Bars indicate the average of two biological replicates, with filled circles indicating the yield from individual experiments.

    Article Snippet: Baker, cat. no. 9344-13) MTSEA-biotin-XX (Biotium, cat. no. 89139-636) Chloroform: Isoamyl alcohol (Fisher Scientific, cat. no. 3160-450ML) Phase-lock gel tube, heavy 1.5 mL (5-Prime, cat. no. FP2302820) Glycogen (AmericanBio, cat. no AB00670-00020) Dynabeads MyOne Streptavidin C1 magnetic beads (Life Technologies, cat. no. 65001) Tris•HCl (Fisher Scientific, cat. no. T5941-500G) Sodium chloride (Sigma Aldrich, cat. no. S9888-2.5KG) Tween-20 (Mp Biomedicals Inc., cat. no. ICN19484180) β-mercaptoethanol (Sigma Aldrich, cat. no. M3148-25ML) Qubit RNA HS assay kit (Thermo Fisher Scientific, cat. no. ) Ambion century plus RNA marker (Thermo Fisher Scientific, cat. no. AM7145) MAXIscript T7 transcription kit (Thermo Fisher Scientific, cat. no. AM1312) Cy5-CTP (GE Healthcare, cat. no. 25-8010-87) 4-thiouridine-5’-triphosphate (s4 UTP) (Trilink Bio, cat. no. N-1025) SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics, cat. no. EC-829) Gel cassettes 1.0 mm (Life Sciences, cat. no. NC2010) Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, cat. no. ND-2000c) MagRack 6 magnetic stand (GE Healthcare, cat. no. 28948964) DynaMag 96 side (Life Technologies, cat. no. 12331D) Qubit 3.0 Fluorometer (Thermo Fisher Scientific, cat. no. ) Rotator (Glas-Col, cat. no. 099A MR1512) Typhoon FLA 9500 (GE Healthcare)

    Techniques: