qubit fluorometric quantitation  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher qubit fluorometric quantitation
    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA <t>DNA</t> yields from the individual U-2 OS cells and the respective controls, as measured by <t>Qubit</t> ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.
    Qubit Fluorometric Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometric quantitation/product/Thermo Fisher
    Average 99 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometric quantitation - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing"

    Article Title: Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0163455

    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.
    Figure Legend Snippet: Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Techniques Used: Whole Genome Amplification, Sequencing, Agarose Gel Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Mutagenesis, Positive Control

    2) Product Images from "TLR and NKG2D Signaling Pathways Mediate CS-Induced Pulmonary Pathologies"

    Article Title: TLR and NKG2D Signaling Pathways Mediate CS-Induced Pulmonary Pathologies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0078735

    CS exposure induces nucleic acid release into the lung. (A) C57BL/6 mice were exposed to FA or CS and total nucleic acid in the BAL fluid was quantified using highly sensitive and highly specific Qubit fluorescent dyes. RNA and DNA levels were significantly greater in CS-exposed mice compared to FA-exposed mice at all times. n = 6 mice. (B) MLE-15 cells were exposed to 20% cigarette smoke extract (CSE) for up to 24 hours. RNA and DNA levels were quantified using highly sensitive and highly specific Qubit fluorescent dyes. RNA and DNA levels were significantly greater in CSE-exposed cells compared to PBS-exposed cells at all times as determined by Student t test. Data are means ± SEM of 3 independent experiments. (C) MLE-15 cells were exposed to 20% cigarette smoke extract (CSE) for up to 24 hours and cell viability was assessed using the MTT assay. Data are means ± SEM of 3 independent experiments. (D) MLE-15 cells were exposed to increasing concentrations of CSE for 24 hrs. Cell-free supernatant, with and without endonuclease (Benzonase) treatment, from exposed cells was applied to reporter cell lines expressing individual TLRs. TLR activation, as measured byNF-κB-induced SEAP activity, was assessed using QUANTI-Blue and by reading the absorbance at 620 nm. Data are shown as the mean ± SEM of three independent experiments.
    Figure Legend Snippet: CS exposure induces nucleic acid release into the lung. (A) C57BL/6 mice were exposed to FA or CS and total nucleic acid in the BAL fluid was quantified using highly sensitive and highly specific Qubit fluorescent dyes. RNA and DNA levels were significantly greater in CS-exposed mice compared to FA-exposed mice at all times. n = 6 mice. (B) MLE-15 cells were exposed to 20% cigarette smoke extract (CSE) for up to 24 hours. RNA and DNA levels were quantified using highly sensitive and highly specific Qubit fluorescent dyes. RNA and DNA levels were significantly greater in CSE-exposed cells compared to PBS-exposed cells at all times as determined by Student t test. Data are means ± SEM of 3 independent experiments. (C) MLE-15 cells were exposed to 20% cigarette smoke extract (CSE) for up to 24 hours and cell viability was assessed using the MTT assay. Data are means ± SEM of 3 independent experiments. (D) MLE-15 cells were exposed to increasing concentrations of CSE for 24 hrs. Cell-free supernatant, with and without endonuclease (Benzonase) treatment, from exposed cells was applied to reporter cell lines expressing individual TLRs. TLR activation, as measured byNF-κB-induced SEAP activity, was assessed using QUANTI-Blue and by reading the absorbance at 620 nm. Data are shown as the mean ± SEM of three independent experiments.

    Techniques Used: Mouse Assay, MTT Assay, Expressing, Activation Assay, Activity Assay

    Related Articles

    RNA Extraction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Sequencing:

    Article Title: Parallelism in eco-morphology and gene expression despite variable evolutionary and genomic backgrounds in a Holarctic fish
    Article Snippet: .. DNA quality and quantity were assessed using agarose gel electrophoresis and the Qubit Fluorometer with the dsDNA BR Assay (Life Technologies). ddRADseq libraries were prepared using a modified version of the Recknagel et al . (2015) [ ] ddRADseq protocol for Illumina sequencing platforms. .. Paired-end 75-bp sequencing was performed on the Illumina NextSeq500 platform at Glasgow Polyomics (University of Glasgow) at 3-4M read coverage per individual.

    Agarose Gel Electrophoresis:

    Article Title: Parallelism in eco-morphology and gene expression despite variable evolutionary and genomic backgrounds in a Holarctic fish
    Article Snippet: .. DNA quality and quantity were assessed using agarose gel electrophoresis and the Qubit Fluorometer with the dsDNA BR Assay (Life Technologies). ddRADseq libraries were prepared using a modified version of the Recknagel et al . (2015) [ ] ddRADseq protocol for Illumina sequencing platforms. .. Paired-end 75-bp sequencing was performed on the Illumina NextSeq500 platform at Glasgow Polyomics (University of Glasgow) at 3-4M read coverage per individual.

    RNA HS Assay:

    Article Title: Fast SARS-CoV-2 detection protocol based on RNA precipitation and RT-qPCR in nasopharyngeal swab samples
    Article Snippet: .. However, a more specific detection of RNA using a Qubit 4 Fluorometer RNA integrity and quantity tests (RNA IQ assay, #Q33221; and RNA HS assay, Q32852, respectively; Invitrogen) did not show any detectable RNA in the range 250 pg/µl to 100 ng/µl (data not shown). ..

    Fluorescence:

    Article Title: Comparison of expression vectors in Lactobacillus reuteri strains
    Article Snippet: .. The activity of the vectors bearing these different promoters was tested in reference strains of Lactococcus lactis, L. reuteri and in five selected strains of L. reuteri , isolated from chicken crop, using a rapid method to detect the GFP fluorescence using the Qubit™ fluorometer (Invitrogen, Milan, Italy), besides the classical direct observation by epifluorescence microscopy and Western blot analysis. .. Bacterial strains, isolation and identification of indigenous lactobacilli from chicken crop Lactococcus lactis spp. cremoris MG1363 ( ) was cultured in GM17 medium (M17 medium supplemented with 0.5% glucose) (Merck KGaA, Darmstadt, Germany) at 30 °C in aerobiosis and L. reuteri DSM 20016T was cultured in MRS medium (Oxoid, Cambridge, UK) in anaerobiosis at 37 °C.

    Isolation:

    Article Title: Comparison of expression vectors in Lactobacillus reuteri strains
    Article Snippet: .. The activity of the vectors bearing these different promoters was tested in reference strains of Lactococcus lactis, L. reuteri and in five selected strains of L. reuteri , isolated from chicken crop, using a rapid method to detect the GFP fluorescence using the Qubit™ fluorometer (Invitrogen, Milan, Italy), besides the classical direct observation by epifluorescence microscopy and Western blot analysis. .. Bacterial strains, isolation and identification of indigenous lactobacilli from chicken crop Lactococcus lactis spp. cremoris MG1363 ( ) was cultured in GM17 medium (M17 medium supplemented with 0.5% glucose) (Merck KGaA, Darmstadt, Germany) at 30 °C in aerobiosis and L. reuteri DSM 20016T was cultured in MRS medium (Oxoid, Cambridge, UK) in anaerobiosis at 37 °C.

    Spectrophotometry:

    Article Title: Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa
    Article Snippet: .. RNA quantity was determined using the ND-1000 Spectrophotometer (NanoDrop ND1000) and its integrity was evaluated using the Qubit™ RNA IQ Assay Kit in the Qubit™ 4 Fluorometer (Thermo Scientific, MA, USA). .. RNA concentrations of 1 µg/µL were used for cDNA synthesis with iScript™ cDNA Synthesis Kit (Bio-Rad, CA, USA) in a T100 Thermal Cycler (Bio-Rad, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Strategies for reducing per‐sample costs in target capture sequencing for phylogenomics and population genomics in plants
    Article Snippet: .. The equipment required for this workflow is a centrifuge capable of 13,000 g , micropipettes (preferably multichannel), −80°C and −20°C freezers, a water bath, a thermocycler, a magnetic bead separator, a Qubit fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA ), and access to a method of hybridization pool quantification (e.g., TapeStation [Agilent Technologies, Santa Clara, California, USA ], BioAnalyzer [Agilent Technologies], qPCR ). ..

    Concentration Assay:

    Article Title: Multiplex Isothermal Amplification Coupled with Nanopore Sequencing for Rapid Detection and Mutation Surveillance of SARS-CoV-2
    Article Snippet: .. We determined the concentration of each RPA 24 samples using a Qubit™ 4 fluorometer with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific 25 Q32851). .. The end-prep reaction was done separately in 15 µl volume using 40 ng of each multiplex RPA 26 samples.

    Quantitation Assay:

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: .. Amplicons Quantitation, Pooling, and SequencingAmplicon DNA concentrations were quantified using the Qubit4.0 Fluorometer (Life Technologies, Stockholm, Sweden). .. Amplicons were combined in equimolar ratios into a single tube with a final concentration of the DNA library of 4 pM.

    Activity Assay:

    Article Title: Comparison of expression vectors in Lactobacillus reuteri strains
    Article Snippet: .. The activity of the vectors bearing these different promoters was tested in reference strains of Lactococcus lactis, L. reuteri and in five selected strains of L. reuteri , isolated from chicken crop, using a rapid method to detect the GFP fluorescence using the Qubit™ fluorometer (Invitrogen, Milan, Italy), besides the classical direct observation by epifluorescence microscopy and Western blot analysis. .. Bacterial strains, isolation and identification of indigenous lactobacilli from chicken crop Lactococcus lactis spp. cremoris MG1363 ( ) was cultured in GM17 medium (M17 medium supplemented with 0.5% glucose) (Merck KGaA, Darmstadt, Germany) at 30 °C in aerobiosis and L. reuteri DSM 20016T was cultured in MRS medium (Oxoid, Cambridge, UK) in anaerobiosis at 37 °C.

    Modification:

    Article Title: Parallelism in eco-morphology and gene expression despite variable evolutionary and genomic backgrounds in a Holarctic fish
    Article Snippet: .. DNA quality and quantity were assessed using agarose gel electrophoresis and the Qubit Fluorometer with the dsDNA BR Assay (Life Technologies). ddRADseq libraries were prepared using a modified version of the Recknagel et al . (2015) [ ] ddRADseq protocol for Illumina sequencing platforms. .. Paired-end 75-bp sequencing was performed on the Illumina NextSeq500 platform at Glasgow Polyomics (University of Glasgow) at 3-4M read coverage per individual.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Western Blot:

    Article Title: Comparison of expression vectors in Lactobacillus reuteri strains
    Article Snippet: .. The activity of the vectors bearing these different promoters was tested in reference strains of Lactococcus lactis, L. reuteri and in five selected strains of L. reuteri , isolated from chicken crop, using a rapid method to detect the GFP fluorescence using the Qubit™ fluorometer (Invitrogen, Milan, Italy), besides the classical direct observation by epifluorescence microscopy and Western blot analysis. .. Bacterial strains, isolation and identification of indigenous lactobacilli from chicken crop Lactococcus lactis spp. cremoris MG1363 ( ) was cultured in GM17 medium (M17 medium supplemented with 0.5% glucose) (Merck KGaA, Darmstadt, Germany) at 30 °C in aerobiosis and L. reuteri DSM 20016T was cultured in MRS medium (Oxoid, Cambridge, UK) in anaerobiosis at 37 °C.

    Recombinase Polymerase Amplification:

    Article Title: Multiplex Isothermal Amplification Coupled with Nanopore Sequencing for Rapid Detection and Mutation Surveillance of SARS-CoV-2
    Article Snippet: .. We determined the concentration of each RPA 24 samples using a Qubit™ 4 fluorometer with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific 25 Q32851). .. The end-prep reaction was done separately in 15 µl volume using 40 ng of each multiplex RPA 26 samples.

    Epifluorescence Microscopy:

    Article Title: Comparison of expression vectors in Lactobacillus reuteri strains
    Article Snippet: .. The activity of the vectors bearing these different promoters was tested in reference strains of Lactococcus lactis, L. reuteri and in five selected strains of L. reuteri , isolated from chicken crop, using a rapid method to detect the GFP fluorescence using the Qubit™ fluorometer (Invitrogen, Milan, Italy), besides the classical direct observation by epifluorescence microscopy and Western blot analysis. .. Bacterial strains, isolation and identification of indigenous lactobacilli from chicken crop Lactococcus lactis spp. cremoris MG1363 ( ) was cultured in GM17 medium (M17 medium supplemented with 0.5% glucose) (Merck KGaA, Darmstadt, Germany) at 30 °C in aerobiosis and L. reuteri DSM 20016T was cultured in MRS medium (Oxoid, Cambridge, UK) in anaerobiosis at 37 °C.

    Hybridization:

    Article Title: Strategies for reducing per‐sample costs in target capture sequencing for phylogenomics and population genomics in plants
    Article Snippet: .. The equipment required for this workflow is a centrifuge capable of 13,000 g , micropipettes (preferably multichannel), −80°C and −20°C freezers, a water bath, a thermocycler, a magnetic bead separator, a Qubit fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA ), and access to a method of hybridization pool quantification (e.g., TapeStation [Agilent Technologies, Santa Clara, California, USA ], BioAnalyzer [Agilent Technologies], qPCR ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Thermo Fisher qubit fluorometric quantitation
    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA <t>DNA</t> yields from the individual U-2 OS cells and the respective controls, as measured by <t>Qubit</t> ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.
    Qubit Fluorometric Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometric quantitation/product/Thermo Fisher
    Average 92 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometric quantitation - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    90
    agilent technologies qubit fluorometric quantitation
    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA <t>DNA</t> yields from the individual U-2 OS cells and the respective controls, as measured by <t>Qubit</t> ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.
    Qubit Fluorometric Quantitation, supplied by agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometric quantitation/product/agilent technologies
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometric quantitation - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Journal: PLoS ONE

    Article Title: Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing

    doi: 10.1371/journal.pone.0163455

    Figure Lengend Snippet: Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Article Snippet: The DNA yield was assessed by Qubit fluorometric quantitation (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Whole Genome Amplification, Sequencing, Agarose Gel Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Mutagenesis, Positive Control