qubit fluorometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Thermo Fisher
    Average 99 stars, based on 916 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples"

    Article Title: Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples

    Journal: 3 Biotech

    doi: 10.1007/s13205-017-1009-x

    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with Qubit. b DNA quantified with NanoDrop. c DNA quality determined by A260/A280 ratio. d DNA quality
    Figure Legend Snippet: Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with Qubit. b DNA quantified with NanoDrop. c DNA quality determined by A260/A280 ratio. d DNA quality

    Techniques Used: DNA Extraction

    2) Product Images from "Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing"

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0135058

    DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER
    Figure Legend Snippet: DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Techniques Used: Modification

    3) Product Images from "Barcoding blood meals: New vertebrate-specific primer sets for assigning taxonomic identities to host DNA from mosquito blood meals"

    Article Title: Barcoding blood meals: New vertebrate-specific primer sets for assigning taxonomic identities to host DNA from mosquito blood meals

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0006767

    Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P
    Figure Legend Snippet: Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P

    Techniques Used: Concentration Assay, Polymerase Chain Reaction, Negative Control, IF-P

    4) Product Images from "Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing"

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0135058

    DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER
    Figure Legend Snippet: DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Techniques Used: Modification

    5) Product Images from "Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds"

    Article Title: Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds

    Journal: Journal of biomaterials applications

    doi: 10.1177/0885328216645952

    DNA content was determined using the Qubit quantitation system. The amount of DNA is represented in micrograms of DNA per gram of dry alginate ± SEM. dsDNA assay sensitivity is 0.2–100 ng per sample. * p
    Figure Legend Snippet: DNA content was determined using the Qubit quantitation system. The amount of DNA is represented in micrograms of DNA per gram of dry alginate ± SEM. dsDNA assay sensitivity is 0.2–100 ng per sample. * p

    Techniques Used: Quantitation Assay, dsDNA Assay

    6) Product Images from "Comparison of expression vectors in Lactobacillus reuteri strains"

    Article Title: Comparison of expression vectors in Lactobacillus reuteri strains

    Journal: FEMS Microbiology Letters

    doi: 10.1111/j.1574-6968.2010.01978.x

    Activity of the three promoters in different species. w.t., Wild type; Erm, pTRKH3- erm GFP transformants; Ldh, pTRKH3- ldh GFP transformants; Slp, pTRKH3- slp GFP transformants. Fluorescent GFP content in lag-phase cell lysates was assessed for the three vectors in Lactococcus lactis MG1363 cultured in GM17 at 30°C, Lactobacillus reuteri DSM 20016 T and L. reuteri N09 both cultured in buffered MRS at 30°C. The fluorescence of each sample was compared with a purified 6xHis-EGFP standard by means of a Qubit ™ fluorometer. The fluorescent GFP content is reported to a similar amount of cells (measured as OD 600 nm ) for each species.
    Figure Legend Snippet: Activity of the three promoters in different species. w.t., Wild type; Erm, pTRKH3- erm GFP transformants; Ldh, pTRKH3- ldh GFP transformants; Slp, pTRKH3- slp GFP transformants. Fluorescent GFP content in lag-phase cell lysates was assessed for the three vectors in Lactococcus lactis MG1363 cultured in GM17 at 30°C, Lactobacillus reuteri DSM 20016 T and L. reuteri N09 both cultured in buffered MRS at 30°C. The fluorescence of each sample was compared with a purified 6xHis-EGFP standard by means of a Qubit ™ fluorometer. The fluorescent GFP content is reported to a similar amount of cells (measured as OD 600 nm ) for each species.

    Techniques Used: Activity Assay, Cell Culture, Fluorescence, Purification

    7) Product Images from "Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing"

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0135058

    DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER
    Figure Legend Snippet: DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Techniques Used: Modification

    8) Product Images from "Demodifying RNA for Transcriptomic Analyses of Archival Formalin-Fixed Paraffin-Embedded Samples"

    Article Title: Demodifying RNA for Transcriptomic Analyses of Archival Formalin-Fixed Paraffin-Embedded Samples

    Journal: Toxicological sciences : an official journal of the Society of Toxicology

    doi: 10.1093/toxsci/kfx278

    Effects of formalin, fixation, and demodification on total RNA, amplifiable RNA, and RNA integrity. A) Total RNA yield as assessed by Nanodrop spectrophotometer and Qubit fluorometer. B) RNA integrity number as measured by Bioanalyzer. C) Amplifiable Actb RNA as measured by reverse transcriptase quantitative PCR of three amplicons across the gene body. *The results of a statistical comparison between OH, 18F, and 3F vs. FR with a p-value
    Figure Legend Snippet: Effects of formalin, fixation, and demodification on total RNA, amplifiable RNA, and RNA integrity. A) Total RNA yield as assessed by Nanodrop spectrophotometer and Qubit fluorometer. B) RNA integrity number as measured by Bioanalyzer. C) Amplifiable Actb RNA as measured by reverse transcriptase quantitative PCR of three amplicons across the gene body. *The results of a statistical comparison between OH, 18F, and 3F vs. FR with a p-value

    Techniques Used: Spectrophotometry, Real-time Polymerase Chain Reaction

    9) Product Images from "Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial"

    Article Title: Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial

    Journal: Journal of Clinical Pathology

    doi: 10.1136/jclinpath-2014-202644

    Variance in DNA recovered by the different extraction methods was calculated using Qubit measurements for engineered samples 1–4. It should be noted that one of the laboratories used a modified version of RecoverAll; however, for the purpose of the analysis, these were treated the same. N refers to the number of laboratories using each method.
    Figure Legend Snippet: Variance in DNA recovered by the different extraction methods was calculated using Qubit measurements for engineered samples 1–4. It should be noted that one of the laboratories used a modified version of RecoverAll; however, for the purpose of the analysis, these were treated the same. N refers to the number of laboratories using each method.

    Techniques Used: Modification

    10) Product Images from "FabV/Triclosan Is an Antibiotic-Free and Cost-Effective Selection System for Efficient Maintenance of High and Medium -Copy Number Plasmids in Escherichia coli"

    Article Title: FabV/Triclosan Is an Antibiotic-Free and Cost-Effective Selection System for Efficient Maintenance of High and Medium -Copy Number Plasmids in Escherichia coli

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0129547

    Plasmid DNA yield. E . coli DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV, pUC19-FabI, and medium-copy number pSA-Hp24-Bla, pSA-Hp24-FabV, pBR322-FabI plasmids and selected on LB agar plates containing 1μM Triclosan (for FabV/FabI plasmids) and or 100μg mL -1 ampicillin (for Bla plasmids). Seed cultures were used to inoculated 5mL LB broth in 50mL flasks and cultured for another 18 hours at 37 or 30°C while shaking at 250rpm. Cell density was then measured by absorbing the diluted samples at 600nm and normalized to 2 OD600. One mL of the normalized culture was then used to extract the plasmid following supplied protocol. The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology). Fig 4A shows plasmid DNA yield when bacteria were grown at 37°C, whereas Fig 4B shows the plasmid DNA yield from cultures incubated at 30°C. Error bars show standard deviations calculated from at-least six (6) independent experiments performed in triplicate.
    Figure Legend Snippet: Plasmid DNA yield. E . coli DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV, pUC19-FabI, and medium-copy number pSA-Hp24-Bla, pSA-Hp24-FabV, pBR322-FabI plasmids and selected on LB agar plates containing 1μM Triclosan (for FabV/FabI plasmids) and or 100μg mL -1 ampicillin (for Bla plasmids). Seed cultures were used to inoculated 5mL LB broth in 50mL flasks and cultured for another 18 hours at 37 or 30°C while shaking at 250rpm. Cell density was then measured by absorbing the diluted samples at 600nm and normalized to 2 OD600. One mL of the normalized culture was then used to extract the plasmid following supplied protocol. The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology). Fig 4A shows plasmid DNA yield when bacteria were grown at 37°C, whereas Fig 4B shows the plasmid DNA yield from cultures incubated at 30°C. Error bars show standard deviations calculated from at-least six (6) independent experiments performed in triplicate.

    Techniques Used: Plasmid Preparation, Transformation Assay, Cell Culture, Incubation

    Related Articles

    RNA Extraction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

    Amplification:

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: .. Amplicons Quantitation, Pooling, and Sequencing Amplicon DNA concentrations were quantified using the Qubit4.0 Fluorometer (Life Technologies, Stockholm, Sweden). .. Amplicons were combined in equimolar ratios into a single tube with a final concentration of the DNA library of 4 pM.

    Isolation:

    Article Title: Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia
    Article Snippet: .. RNA was isolated using Zymo Research Direct-zol MiniPrep kit (Cat #R2050) and quantified using Invitrogen Qubit RNA Broad Range Assay kit (Cat #Q10210) and Invitrogen Qubit 2.0 Fluorometer (Cat # Q32866). .. Clontech RNA to cDNA EcoDry Premix (Double Primed) (Cat #639548) was used to reverse transcribe the extracted RNA.

    Purification:

    Article Title: Characterization and Genome Analysis of a Phthalate Esters-Degrading Strain Sphingobium yanoikuyae SHJ
    Article Snippet: .. The purified library concentrations were determined by Qubit 2.0 Fluorometer (Cat # , Life Technologies). .. Sequencing primers were annealed to the SMRTbell templates followed by binding with the complex using the DNA/Polymerase Binding kit P6 with the magBead loading kit (Pacific Biosciences).

    Sequencing:

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: .. Amplicons Quantitation, Pooling, and Sequencing Amplicon DNA concentrations were quantified using the Qubit4.0 Fluorometer (Life Technologies, Stockholm, Sweden). .. Amplicons were combined in equimolar ratios into a single tube with a final concentration of the DNA library of 4 pM.

    Concentration Assay:

    Article Title: Impact of Rearing Conditions on the Ambrosia Beetle’s Microbiome
    Article Snippet: .. Library concentration was quantified using a DNA HS kit (Invitrogen, Carlsbad, CA, USA, cat. No. Q32854) in a Qubit 2.0 fluorometer (Invitrogen, cat. No. Q32866). .. Adapter ligation and library size were evaluated using a Bioanalyzer (Agilent, Santa Clara, CA, USA, cat. No. G2953CA) DNA HS chip (Agilent, cat. No. 5067-4626).

    Article Title: Genetic screen in myeloid cells identifies TNF-α autocrine secretion as a factor increasing MDSC suppressive activity via Nos2 up-regulation
    Article Snippet: .. The RNA concentration was determined using a Qubit 4 Fluorometer (Thermo Fisher Scientific, Waltham) and 1 µg total RNA was used for cDNA synthesis. .. The mRNA was transcribed in cDNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, #4368814) according the manufacturer’s instructions.

    Quantitation Assay:

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: .. Amplicons Quantitation, Pooling, and Sequencing Amplicon DNA concentrations were quantified using the Qubit4.0 Fluorometer (Life Technologies, Stockholm, Sweden). .. Amplicons were combined in equimolar ratios into a single tube with a final concentration of the DNA library of 4 pM.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine
    Article Snippet: .. Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852). .. The cDNA was prepared with the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, Cat # E6300S) using oligo dT as a primer.

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    Thermo Fisher qubit fluorometer
    Mean (±SEM) <t>DNA</t> concentration (nM) of PCR products, measured by <t>Qubit</t> fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P
    Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Thermo Fisher
    Average 99 stars, based on 916 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher qubit fluorometric quantitation
    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA <t>DNA</t> yields from the individual U-2 OS cells and the respective controls, as measured by <t>Qubit</t> ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.
    Qubit Fluorometric Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometric quantitation/product/Thermo Fisher
    Average 95 stars, based on 223 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometric quantitation - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    99
    Thermo Fisher qubit 3 0 fluorometer
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit 3 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 3 0 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    qubit 3 0 fluorometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Barcoding blood meals: New vertebrate-specific primer sets for assigning taxonomic identities to host DNA from mosquito blood meals

    doi: 10.1371/journal.pntd.0006767

    Figure Lengend Snippet: Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P

    Article Snippet: For each PCR product, the remaining volume was sent to the University of Florida, Interdisciplinary Center for Biotechnology Research (ICBR) for DNA quantification by Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Concentration Assay, Polymerase Chain Reaction, Negative Control, IF-P

    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Journal: PLoS ONE

    Article Title: Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing

    doi: 10.1371/journal.pone.0163455

    Figure Lengend Snippet: Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Article Snippet: The DNA yield was assessed by Qubit fluorometric quantitation (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Whole Genome Amplification, Sequencing, Agarose Gel Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Mutagenesis, Positive Control

    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) . Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry

    Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry